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Citations for Roche :
8351 - 8400 of 9048 citations for Cow WAP kazal immunoglobulin kunitz and NTR domain containing protein 2 WFIKKN2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... and the whole converted DNA was used as input for library preparation using the Accel-NGS Methyl-Seq DNA Library Kit (Swift Biosciences) according to the recommended protocol and indexing PCR using 2X KAPA HiFi HotStart Uracil+ ReadyMix (KAPA Biosystems). Indexed ...
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bioRxiv - Synthetic Biology 2022Quote: ... The PCR products from each level were pooled in equimolar concentration and the pool was converted into a sequence-ready library with the Kapa Hyper Library Construction kit (Roche, CA) with no PCR amplification ...
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bioRxiv - Molecular Biology 2020Quote: ... The entire ChIP DNA or 10 ng DNA in the case of ChIP input was used to make DNA library with the KAPA Hyper Prep Kit (KAPA Biosystems) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2022Quote: Genomic DNA from OS/PDB tissues and matched healthy bone marrow samples was isolated using the High Pure PCR Template Preparation Kit following the manufacturer’s instructions (Roche Life Science). Genomic DNA from cell lines (MC3T3 and RPE1 ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA was synthesized from 1 μg total RNA for each sample using the Transcriptor High Fidelity cDNA Synthesis Kit (Roche, 05081963001), with a mixture of oligo dT and random primers ...
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bioRxiv - Genomics 2020Quote: ... The libraries were visualized with the Fragment Analyzer (Advanced Analytical Technologies) and quantified using the Kapa library quantification kit for Illumina platforms (Kapa Biosystems). [3] Based on the concentration ...
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bioRxiv - Genomics 2019Quote: ... was the substrate for a 10 μL qPCR reaction using either Taq DNA polymerase or KAPA HiFi (KAPA HiFi HotStart PCR kit, Kapa Biosystems) according to the manufacturer’s protocol with barcoded P5 and P7 sequencing primers at 0.5 mM and with 1x EvaGreen dye (Biotium ...
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bioRxiv - Immunology 2021Quote: ... and used as a template for the synthesis of cDNA utilizing the Transcriptor First Strand cDNA Synthesis Kit (Roche, Basel, Switzerland). Total genomic DNA was extracted by boiling BMDCs in a 50 mM NaOH solution as previously described (47) ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized with 12 µL of RNA and anchored oligo(dT)18 primer in a 20 µL reaction using the Transcriptor First Strand cDNA synthesis kit at 55°C for 30 min for reverse transcriptase reaction (Roche Diagnostics).
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bioRxiv - Immunology 2020Quote: ... Tagmented DNA was ligated to unique i5/i7 barcoded primer combinations using the Illumina Nextera XT Index Kit v2 and KAPA HiFi HotStart ReadyMix (2X; KAPA Biosystems) and then purified using AmPure Beads XP (Agencourt) ...
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bioRxiv - Pathology 2019Quote: ... cDNA synthesis was performed with a 1 μg RNA sample using the First Strand cDNA Synthesis kit RT-PCR (Roche, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... 300 ng of total RNA from three biological replicates per B cell subset was used as input for the KAPA stranded mRNA-seq Kit with mRNA capture beads (KAPA Biosystems). Libraries were assessed for quality on a bioanalyzer ...
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bioRxiv - Developmental Biology 2019Quote: ... Sample preparation protocol for the RNASeq libraries was following the manufacturer’s recommendations of KAPA Stranded mRNA-Seq Illumina® Platforms Kit (Roche-Kapa Biosystems). The libraries were sequenced on HiSeq2500 (Illumina ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 0.05% (v/v) Tween-20) and processed through the spin columns from High Pure Viral Nucleic Acid Large Volume kits (Roche, Switzerland). Purified DNA was eluted in either 45 µl of EB buffer (10 mM tris-hydrochloride (pH 8.0 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: Total RNA was isolated at day 7 or 14 of MSCs differentiation using the High Pure RNA Isolation kit (Roche Diagnostics). For tissues ...
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bioRxiv - Plant Biology 2019Quote: ... An Illumina DNAseq library was constructed for polishing the PacBio based assembly using 1ug of DNA the same high molecular weight DNA prep with the KAPA HyperPrep Kit (Kapa Biosystems). The Illumina DNAseq library was sequenced on an Illumina HiSeq4000 under paired end mode (150 bp ...
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bioRxiv - Genomics 2019Quote: ... Libraries were quantified for normalization by qPCR using the KAPA Library Quantification Kit for Illumina sequencing platforms (KAPA Biosystems, Boston, MA). The final libraries were also assessed using the DNA1000 kit for the Bioanalyzer 2100 (Agilent Technologies ...
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bioRxiv - Developmental Biology 2019Quote: ... 1 µg of linearized pCR©II-Tc-mef2 or pCR©II-Tc-duf/kirre was in vitro transcribed utilizing the DIG RNA labelling kit (Roche) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... All reverse transcriptase qPCR assays for a given target were performed simultaneously for all samples using the LightCycler Multiplex RNA Virus Master Kit (Roche diagnostics). Each sample was normalized with the geometric mean of the quantification cycle (Cq ...
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bioRxiv - Immunology 2019Quote: ... cDNA libraries were quantitated using qPCR in a Roche LightCycler 480 with the Kapa Biosystems kit for library quantitation (Kapa Biosystems) prior to sequencing.
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bioRxiv - Immunology 2019Quote: ... and the quantity and quality of each pool was assessed by the Fragment Analyzer and subsequently by qPCR using the Illumina Library Quantification Kit (Kapa Biosystems) on a Light Cycler LC480II (Roche ...
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bioRxiv - Genomics 2019Quote: ... Quality control for primer target amplification and performance in multiplex PCR was assessed by screening each primer pair in singleplex reactions using gDNA from PC3 or DU145 cells (10 ng) as template (see below) using the HiFi HotStart Uracil+ ReadyMix PCR Kit (Kapa Biosystems). Reactions were supplemented with uracil N-glycosylase (0.2 U ...
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bioRxiv - Cell Biology 2019Quote: ... Libraries were prepared by the Genome Technologies core facility at The Jackson Laboratory using the Ovation RNA-seq System V2 (Nugen) and Hyper Prep Kit (Kapa Biosystems). Briefly ...
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bioRxiv - Genomics 2019Quote: TUNEL staining on rat β-cells was performed 48h after transfection using the TMR red In Situ Cell Death Detection Kit (Roche) combined to polyclonal guinea pig anti-insulin (dilution 1:40 ...
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bioRxiv - Cell Biology 2020Quote: ... Libraries were prepared by the Genome Technologies core facility at The Jackson Laboratory using the Ovation RNA-seq System V2 (NuGEN Technologies) and Hyper Prep Kit (Kapa Biosystems). Libraries were checked for quality and concentration using the D5000 ScreenTape assay (Agilent Technologies ...
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bioRxiv - Microbiology 2019Quote: ... 50 ng of DNA from each sample was pooled with multiplex identifiers for 2-region 454 sequencing on GS FLX Titanium PicoTiterPlates (70675) with the use of a GS FLX Titanium Sequencing Kit XLR70 (Roche Diagnostics). Library construction and 454 pyrosequencing were performed at the National High-Throughput DNA Sequencing Centre ...
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bioRxiv - Microbiology 2019Quote: ... Quantitative reverse transcription PCR was performed using Eppendorf Realplex2 Mastercycler with reaction mixtures prepared with KAPA SYBR Fast Universal qPCR Kit (KAPA Biosystems). The PCR primers for KIF1A ...
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bioRxiv - Biochemistry 2019Quote: ... The cDNA was then used to perform qRT PCR using the KAPA SYBR FAST qRT PCR master mix kit (KK4602, KAPA Biosystems) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: Poly-A mRNA was purified from total RNA using oligodT magnetic beads and strand-specific indexed libraries were prepared using the KAPA Stranded RNA-Seq kit followed by ten cycles of amplification using KAPA HiFi DNA polymerase (KAPA Biosystems). Libraries were quantified and pooled based on a post-PCR Agilent Bioanalyzer and 75 bp paired-end reads were generated on the Illumina HiSeq v4 following the manufacturer’s standard sequencing protocols (as above).
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bioRxiv - Bioengineering 2021Quote: ... 1μg of total RNAs were used to prepared ribo-minus RNA-seq libraries using the KAPA Stranded RNA-Seq Kit with RiboErase (Roche, KK8484), and the depletion of rRNAs confirmed by fluorometric quantification using the Qubit RNA HS Assay kit (Invitrogen) ...
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bioRxiv - Genomics 2021Quote: ... target sites were amplified directly from genomic DNA in the lysate using a KAPA HiFi HotStart PCR kit (KAPA Biosystems, KK2501) as previously described18 ...
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bioRxiv - Genomics 2021Quote: ... 1-8 ng of ChIPed DNA was treated with end-repair, A-tailing, and ligation of Illumina compatible adapters (IDT, Inc) using the KAPA-Illumina library creation kit (KAPA biosystems). The ligated products were enriched with 8-10 cycles of PCR (HiFi premix ...
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bioRxiv - Genomics 2021Quote: Genomic DNA libraries were constructed for high throughput sequencing using the KAPA hyper Prep Library Preparation Kit (Kapa Biosystems, Woburn, MA). Mechanical DNA shearing was performed with the Covaris S220 through microTube-50 AFA Fiber Screw-Cap (Covaris® ...
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bioRxiv - Developmental Biology 2020Quote: ... Library preparation for ChIP-sequencing (ChIP-seq) was performed as described 66 using the KAPA LTP Library Preparation Kit (KAPA Biosystems) and 2 ng of input DNA ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were washed twice in cold PBS and total RNA was extracted using a High Pure RNA isolation kit (Roche 11828665001). Reverse transcribed RNA was used for sequencing by the University of Lausanne ...
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bioRxiv - Cancer Biology 2021Quote: ... 5% CO2 by quantification of LDH released into cell supernatants by apoptotic/necrotic cells (LDH detection kit, Roche Applied Science, #11644793001). Maximal lysis of the target cells (= 100% ...
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bioRxiv - Immunology 2021Quote: ... Tagmented DNA was ligated to a unique i5/i7 barcoded primer combinations using the Illumina Nextera XT Index Kit v2 and KAPA HiFi HotStart ReadyMix (Roche, #07958927001), and then purified using AmPure Beads XP (Beckman Coulter ...
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bioRxiv - Microbiology 2021Quote: ... the DNA was ligated to i5/i7 barcoded primers using the Illumina Nextera XT Index Kit v2 and KAPA HiFi HotStart ReadyMix (2X; KAPA Biosystems). Next the DNA was purified using AmPure Beads XP (Agencourt) ...
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bioRxiv - Neuroscience 2020Quote: ... Five hundred ng of RNA for each sample were reverse transcribed using the Transcriptor first strand cDNA synthesis kit (Roche, Germany). For real-time quantitative PCR reactions ...
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bioRxiv - Neuroscience 2020Quote: TUNEL staining was performed according to the manufacturer’s instructions (In Situ Cell Death Detection Kit, Fluorescein, Roche, #11 684 795 910). In brief ...
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Cell Ecosystem and Signaling Pathways of Primary and Metastatic Pediatric Posterior Fossa EpendymomabioRxiv - Cancer Biology 2020Quote: ... cDNA quality of the libraries was evaluated with a bioanalyzer and quantified using a KAPA Library Preparation Kit (Roche sequencing, KK4824). Libraries were sequenced using an Illumina NextSeq 500 on high output mode with 20 bp (read 1 ...
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bioRxiv - Neuroscience 2021Quote: Cytotoxicity in the cell cultures (primary cortical neurons and cell lines) was assessed using the cytotoxicity lactate dehydrogenase (LDH) detection kit according to the manufacturer’s instructions (Roche, Basel, Switzerland). Furthermore ...
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bioRxiv - Immunology 2020Quote: ... For immunohistochemistry paraffin-embedded normal human skin and ES was stained for human Cytokeratin 5/6 according to the manufacturer’s protocol using a Ventana BenchMark Series automated slide stainer with ultraView Universal DAB Detection kit (Roche, 760-500).
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bioRxiv - Neuroscience 2020Quote: ... Libraries were prepared by the Genome Technologies core service at The Jackson Laboratory using the KAPA RNA Hyper Prep Kit with RiboErase (HMR) (KAPA Biosystems), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... The selected fragments were then end repaired, A-tailed and ligated to Illumina compatible adapters (IDT, Inc) using KAPA Illumina library creation kit (KAPA biosystems). Libraries were quantified using KAPA Biosystem’s next-generation sequencing library qPCR kit and run on a Roche LightCycler 480 real-time PCR instrument ...
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bioRxiv - Bioengineering 2020Quote: ... The culture supernatant for each condition was harvested for RNA extraction using the HP Viral RNA Kit (Roche, Cat no. 11858882001) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... pGEM clones were linearized with NotI and transcribed with SP6 or T7 RNA polymerase using the DIG RNA labelling Kit (Roche 1277073) to generate antisense or sense probes ...
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bioRxiv - Neuroscience 2020Quote: ... The resulting PCR products were purified and used as templates for in vitro transcription by employing DIG RNA Labeling Kit (SP6/T7) (Roche #11175025910). The resulting RNAs generated from SP6-mediated in vitro transcription were used as positive probes (cRNA ...
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bioRxiv - Molecular Biology 2021Quote: 200 μL of eluate was extracted on the Magna Pure 96 using a DNA and Viral NA Small Volume Kit (Roche, 06543588001) with the universal small volume protocol and eluted into 50 μL proprietary elution buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... and samples (100 ng) were input in the RNA library construction using the KAPA RNA Hyper library prep kit (Roche, Switzerland). Customized adapters with unique molecular indexes (UMI ...