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Citations for Roche :
751 - 800 of 2419 citations for SARS CoV 2 Spike E M Mosaic Protein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: Fresh pre-cut pancreatic tissue (2 × 2 mm) were digested with a solution of collagenase P in HBSS-1% HEPES (0.75 mg/mL, Roche) at 37°C for 7 min with shaking ...
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bioRxiv - Developmental Biology 2021Quote: ... the embryos are rinsed in TBS/0.1% Tween-20 (TBST) then blocked for 2 h in 2% blocking solution (Roche) and incubated O/N in the same solution containing 1:2,500 anti-digoxigenin antibody (Roche) ...
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bioRxiv - Plant Biology 2023Quote: ... in 100μl extraction buffer (Tris-HCl 50 mM pH 6.8, SDS 2%, DTT 2 mM and 1× protease inhibitors (Roche) and centrifuged for 5 min at 13,000g at 4OC ...
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bioRxiv - Immunology 2023Quote: ... cells were lysed in 100μL or 200μl of 2% SDS lysis buffer (2% SDS, 50mM Tris-HCl pH 7.5, 5mM EDTA, 15U/mL DnaseI (Roche), cOmplete mini EDTA-free protease inhibitor tablet (Roche ...
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bioRxiv - Cell Biology 2019Quote: ... proteins were detected using ECL lumi-light plus (Roche) and images acquired using camera system (Chemitouch Biorad).
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bioRxiv - Cell Biology 2022Quote: ... Proteins were transferred to nitrocellulose or PVDF membranes (Roche) in tris-glycine buffer at 110 V for 1 h ...
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bioRxiv - Molecular Biology 2021Quote: ... and protein extracted with ELB buffer containing PhosSTOP (Roche) and 50 μM sodium fluoride ...
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bioRxiv - Cell Biology 2019Quote: ... protein inhibitor and phosphatase inhibitor (Complete-EDTA free, Roche/Sigma prod ...
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bioRxiv - Cancer Biology 2019Quote: BSA Protein Assay Kit (A8020-5, Roche, Basel, Switzerland) was applied to measure the protein concentration after lysing BC cells with RIPA buffer ...
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bioRxiv - Developmental Biology 2019Quote: ... 1/10 volume of Protein-G agarose beads (Roche) was then added and the sample rotated for 30 min at RT °C ...
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bioRxiv - Immunology 2019Quote: Proteins were digested on beads using trypsin (Roche, Germany) in 6 M Urea ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were transferred onto polyvinylidene fluoride (PVDF) membrane (Roche) for 80V for 1.5 hours ...
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bioRxiv - Biochemistry 2019Quote: ... and then incubated with protein A–agarose beads (Roche) for 4 h ...
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bioRxiv - Genomics 2019Quote: ... protease inhibitors) and precleared with protein A beads (Roche) at 4 °C for 1 hr ...
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bioRxiv - Microbiology 2021Quote: ... Immunoprecipitations were performed using Protein A agarose beads (Roche). DivJ was immunoprecipitated using an anti-DivJ antiserum (15) ...
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bioRxiv - Cancer Biology 2021Quote: ... at 4 °C overnight and protein G-Agarose (Roche) at 4 °C for 3 h ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were semi-dry blotted onto PVDF membranes (Roche), which were subsequently blocked in 5 % milk in PBST ...
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bioRxiv - Cell Biology 2021Quote: ... followed by incubation with Protein G beads (Roche, 1124323301) for 2 hours at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were transferred onto polyvinylidene fluoride (PVDF) membrane (Roche) for 30V for 16 hours ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were reduced using 10 mM dithiothreitol (Roche) and alkylated with 50 mM iodoacetamide ...
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bioRxiv - Neuroscience 2021Quote: ... Then homogeneous protein G-agarose suspension was added (Roche) to the suspension ...
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bioRxiv - Pathology 2019Quote: ... Proteins were detected using polyclonal antibodies against GFP (Roche), Histone H3 (GeneTex ...
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bioRxiv - Plant Biology 2021Quote: ... coupled to 50µl bed volume Protein A agarose (Roche) per sample ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 mM DTT and 1x protein inhibitor cocktail (Roche), and incubated at 25ºC for 1 h on a rotating wheel ...
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bioRxiv - Neuroscience 2021Quote: ... followed by incubation with protein G-agarose beads (Roche) for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins were transferred onto PVDF membranes (Roche, Basel, Switzerland), and Western blot detection was carried out as previously described [32,33] ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were reduced using 10 mM dithiothreitol (Roche) and alkylated with 50 mM iodoacetamide ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were reduced using 10 mM dithiothreitol (Roche) and alkylated with 50 mM iodoacetamide ...
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bioRxiv - Cancer Biology 2023Quote: ... Following electrophoretic transfer of proteins onto PVDF membranes (Roche), nonspecific binding was blocked by incubation with 5% BSA ...
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bioRxiv - Cancer Biology 2023Quote: ... 400 µg of protein were reduced using DTT (Roche, Cat#10708984001 ...
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bioRxiv - Developmental Biology 2024Quote: ... and incubated with protein A agarose beads (Roche, #11134515001) at 4 °C overnight with shaking ...
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bioRxiv - Cancer Biology 2024Quote: ... Total protein were extracted from cells and tissues using protein lysis buffer supplemented with protease and phosphatase inhibitors (Roche Applied Science, Penzberg, Germany). Protein concentrations were assayed by the Pierce™ BCA Protein Assay Kit (Thermo Scientific ...
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bioRxiv - Plant Biology 2019Quote: ... The fine powder was resuspended with the extraction buffer (50 mm Tris, pH 8, 8 M urea, and 0.5% SDS) supplemented with protease inhibitor (Roche Diagnostics GmbH, Mannheim, Germany), and homogenized with a Dounce homogenizer ...
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bioRxiv - Cell Biology 2020Quote: Cell proteins were extracted by Pierce™ Classic Magnetic IP/Co-IP Kit (Thermo Scientifi, 88804) or Complete™ Lysis-M EDTA-free kit (Roche, 04719964001) according to the manuals ...
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bioRxiv - Biochemistry 2020Quote: ... The spheroplasts were resuspended in ice-cold D-buffer (0.6 M sorbitol, 10 mM Tris-HCl pH 7.4) containing protease inhibitors (complete protease inhibitor cocktail, Roche; 1 tablet per 50 mL), and lysed by 10 strokes in a Dounce homogenizer ...
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bioRxiv - Neuroscience 2022Quote: ... They were scooped with a brush and dropped in a glass tube containing the homogenization buffer (HB; 0.32 M sucrose and one tablet Complete mini EDTA-free protease inhibitor cocktail (Roche; 10 ml, pH 7.4)) at 4°C (50 μl of buffer for each slice) ...
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bioRxiv - Neuroscience 2020Quote: Genome-wide methylation analysis was performed using the Roche SeqCap Epi Developer M Enrichment system on bisulfite-treated DNA (Roche Nimblegen; WI, USA), with custom designed primers targeting promoter and enhancer regions of the genome ...
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bioRxiv - Neuroscience 2022Quote: ... the dishes were placed on ice and 500 μl of homogenization buffer (HB; 0.32 M sucrose and one tablet Complete mini EDTA-free protease inhibitor cocktail (Roche; 10 ml, pH 7.4)) (4°C ...
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bioRxiv - Microbiology 2021Quote: ... monocytes were harvested using ice-cold lysis buffer (1% Triton X-100, 2% SDS, 150 mM NaCl, 10 mM HEPES, 2 mM EDTA containing protease inhibitor cocktail - Roche). Cell lysates were heated at 100 °C for 5 min in the presence of Laemmli buffer (20% β-mercaptoethanol ...
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bioRxiv - Genetics 2021Quote: ... each sample was amplified in 6/2 PCR reactions (2 µg DNA/reaction) in the primary/secondary screens using the ReadyMix Kapa polymerase (Roche) with a total of 20 cycles and an annealing temperature of 55°C (Primer sequences in Table S4 ...
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bioRxiv - Biochemistry 2021Quote: ... 2×106 cells HEK293T cells were transfected with 2 μg plasmid DNA using X-tremeGENE HP DNA Transfection Reagent (Roche) in 10-cm dishes ...
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bioRxiv - Plant Biology 2022Quote: ... 150 mM NaCl, 20 mM KCl, 2 mM MgCl2, 1% TX-100, 40U Ribolock ml-2 and protease inhibitor cocktail, Roche) followed by clearing at 17 000 × g for 10 min at +4C° ...
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bioRxiv - Immunology 2023Quote: ... dissociated from the coverslips in 8 M urea lysis buffer (100 mM NaCl, 25 mM Tris, 2% SDS, 0.1% tween 20, 2 mM EDTA, 0.2 mM PMSF, and 1x Roche cOmpleteProtease inhibitor). Lysates were treated with benzonase (Sigma ...
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DTX3L and USP28 fine-tune DNA double strand repair through mutual regulation of their protein levelsbioRxiv - Biochemistry 2023Quote: ... 1% Triton X-100, 2 mM EDTA, 2 mM EGTA, 1 mM PMSF, and complete protease inhibitor cocktail tablet; Roche), incubated with continuous shaking at 4°C for 20 min and then centrifuged at 12 000xg at 4°C for 15 min ...
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bioRxiv - Cell Biology 2024Quote: ... The parasites were pelleted by centrifugation at 25,000 x g and resuspended in 5 × the pellet volume with hypotonic lysis buffer (1 mM HEPES-NaOH pH 7.4, 2 mM EGTA, 2 mM DTT with protease inhibitor cocktail; cOmplete™, EDTA-free, Roche). The parasite suspension was incubated for 10 min on ice and then lysed by approximately 30 passages through a 1mL syringe fitted with a 27G needle.
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bioRxiv - Cancer Biology 2021Quote: ... 2 mM EDTA with 1x protease inhibitors (Roche). Protein concentrations were quantified with a BCA Kit (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: ... For cDNA synthesis 2 μg of DNaseI (Roche) treated total RNA was reverse transcribed using M-MuLV reverse transcriptase (Minotech) ...
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bioRxiv - Genetics 2019Quote: ... 2 mM benzamidine and protease inhibitor cocktail (Roche)] ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 µl 5x KAPA HiFi buffer (Kapa Biosystems), 0.3 µl 10 mM dNTPs (Kapa Biosystems) ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 mM 2-mercaptoethanol) with protease inhibitors (Roche) and 300 U/L benzonase (Sigma) ...