Labshake search
Citations for Roche :
751 - 800 of 1350 citations for Recombinant Human CD19 protein His tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2019Quote: CISH riboprobes were generated from the gDNA or cDNA with PCR and labeled with digoxigenin (DIG) using DIG RNA labeling mix (Roche, 11277073910) by in vitro transcription ...
-
bioRxiv - Plant Biology 2019Quote: ... were used as templates to synthesis DIG-labeled probes according to the manufacturer’s protocol (DIG High Prime DNA Labeling and Detection Starter Kit II, Roche, Basel, Switzerland). ULTRAhyb®-Oligo Hybridization Buffer (Ambion ...
-
bioRxiv - Microbiology 2020Quote: ... were amplified using primers Sb.1 - Sb.2 and Sb.3 to Sb.8 (Table 2) and labeled with digoxigenin (DIG) using the PCR DIG probe synthesis kit (Roche Diagnostics). Membranes were hybridized with these probes at 65°C and then washed at 68°C with decreasing concentrations (from 2X to 0.2X ...
-
bioRxiv - Neuroscience 2021Quote: ... Sections were then hybridized overnight at 70 °C with a digoxigenin (DIG)-labeled RNA probes (DIG RNA labeling kit, Roche 11277073910) for Gadd45b mRNA ...
-
bioRxiv - Molecular Biology 2020Quote: ... A particular tRNA was hybridized with a corresponding antisense oligo DNA probe shown in Table S1 labeled with digoxigenin (DIG) using DIG Oligonucleotide Tailing Kit (Roche Diagnostics) in Hybridization Solution [0.50 M Na2HPO4 ...
-
bioRxiv - Molecular Biology 2022Quote: CISH riboprobes for Spp1 and Myh9 were generated from gDNA or cDNA with PCR and labeled with digoxigenin (DIG) using DIG RNA labeling mix (Roche, 11277073910) by in vitro transcription ...
-
bioRxiv - Genomics 2023Quote: ... was indirectly labeled by PCR in the presence of digoxigenin-11-dUTP detected by antidigoxigenin-fluorescein isothiocyanate (FITC; both from Roche Diagnostics). The remaining satDNAs (‘pTS5’ ...
-
bioRxiv - Immunology 2023Quote: ... rs9500880 and rs1921622 was conducted by PCR utilizing fluorescent-labeled hybridization FRET probes followed by melting curve analysis in a LightCycler 480 (Roche Diagnostics). The following probes and primers were used ...
-
bioRxiv - Zoology 2023Quote: The PCR products amplified from both IHHNV-309 and IHHNV-389 methods were used as templates to prepare digoxigenin (DIG)-labeled IHHNV probes using a PCR DIG-labeling kit (Roche, Germany). After preparation ...
-
bioRxiv - Physiology 2024Quote: ... Fluorescein-labeled cRNA probes for esr1 and esr2a were generated by in vitro transcription using Fluorescein RNA Labeling Mix (Roche Diagnostics), SP6 or T7 RNA polymerase (Roche Diagnostics) ...
-
bioRxiv - Molecular Biology 2023Quote: ... and fluorescein (FITC)-labeled riboprobes were synthesized using T7 or SP6 polymerase along with a DIG or FITC labeling mix (Roche Diagnostics). For the detection of hybridization signals ...
-
bioRxiv - Neuroscience 2023Quote: ... and incubated at room temperature for 30 minutes with HRP-labeled sheep anti-FITC antibody (1/500 in TNB, Roche/Sigma). Following washes in TNT ...
-
bioRxiv - Genomics 2024Quote: ... and 22-bp Sat III were synthesized as probes and labeled with Dig-dUTP or Bio-dUTP (Roche Diagnostics, Basel, Switzerland) using the Biotin and Dig Nick Translation Mix (Roche ...
-
bioRxiv - Cancer Biology 2021Quote: ... Pooled sample libraries were hybridized with SeqCap EZ Human Exome v3.0 probes (Roche) for WES ...
-
bioRxiv - Molecular Biology 2023Quote: ... 3×10−4 M MTG and 300 μg/ml human transferrin (Roche, 10652202001)) in a triple vent petri 10 cm dishes (Thermo fisher ...
-
bioRxiv - Immunology 2024Quote: Human monocytes were lysed using RIPA buffer supplemented with protease inhibitor cocktail (Roche) and phosphatase inhibitors (Sigma-Aldrich) ...
-
bioRxiv - Cancer Biology 2024Quote: ... 1% Penicillin/Streptomycin and 20 IU human IL-2 (Roche Diagnostics, Mannheim, Germany) for 5d ...
-
bioRxiv - Neuroscience 2022Quote: ... 2X SSC was replaced with 1X DNase buffer for 5 min and then a 1:50 dilution of DNase I in DNase buffer (DNase I recombinant, RNase-free, Roche, Cat. No. 04716728001), and incubated for 1 hour at 37°C ...
-
bioRxiv - Neuroscience 2019Quote: ... 2X SSC was replaced with 1X DNase buffer for 5 min and then a 1:50 dilution of DNase I in DNase buffer (DNase I recombinant, RNase-free, Roche, Cat. No. 04716728001), and incubated for 1 hour at 37°C ...
-
bioRxiv - Molecular Biology 2021Quote: ... sections were fixed with 4% PFA (PierceTM, 28908) in PBS for 15 minutes at room temperature and permeabilized with PCR grade recombinant Proteinase K (Roche Applied Science, 3115887001) for 30 minutes at 37 °C ...
-
bioRxiv - Cancer Biology 2021Quote: ... 25 μL of Protein G agarose beads (Roche) were placed into each IP sample and incubated o/n at 4 °C on a rotator ...
-
bioRxiv - Cell Biology 2020Quote: ... The protein was digested with AspN protease (Roche) at 30°C for 16 hr ...
-
bioRxiv - Plant Biology 2019Quote: ... and a protein phosphatase inhibitor tablet (PhosSTOP, Roche). Protein samples were analysed by Western blot with a mouse anti-GFP HRP-conjugated antibody.
-
bioRxiv - Plant Biology 2019Quote: ... and a protein phosphatase inhibitor tablet (PhosSTOP, Roche). After centrifuge ...
-
bioRxiv - Plant Biology 2019Quote: ... and a protein phosphatase inhibitor tablet (PhosSTOP, Roche). After addition of half a volume of SDS loading dye ...
-
bioRxiv - Cell Biology 2021Quote: ... Protein bands were detected using Lumi-Light (Roche) chemiluminescence kit as per the manufacturer’s instructions.
-
bioRxiv - Evolutionary Biology 2021Quote: ... 1x protein inhibitor cocktail (cOmplete, Roche; added fresh), 300μg/ml lysozyme ...
-
bioRxiv - Plant Biology 2021Quote: ... and a protein phosphatase inhibitor tablet (PhosSTOP, Roche). After addition of SDS loading buffer ...
-
bioRxiv - Cancer Biology 2020Quote: ... Proteins were transferred onto Polyvinylidenfluorid (PVDF) membranes (Roche), blocked with 5% dry milk for 1 hour and incubated with primary antibodies overnight at 4°C ...
-
bioRxiv - Plant Biology 2019Quote: ... GUN1 protein was expressed in RTS ProteoMaster (Roche). Full-length GUN1 cDNA and N-terminal truncated versions as EcoRI-SalI PCR fragments were ligated into pET48b (Novagen ...
-
bioRxiv - Molecular Biology 2019Quote: ... protein was degraded by Proteinase K (3115879001, Roche), and a phenol/chloroform/isoamyl alcohol DNA extraction ...
-
bioRxiv - Cell Biology 2019Quote: ... A Protein A agarose bead slurry (Sigma-Roche) (60ul ...
-
bioRxiv - Molecular Biology 2020Quote: ... Protein-G agarose beads were manufactured by Roche and purchased from Sigma Aldrich ...
-
bioRxiv - Genomics 2021Quote: ... 750 μL of Protein G Agarose beads (Roche) were used to capture 400 μL of SUMO-2/3 antibody (8A2 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Protein-G agarose beads were manufactured by Roche. All other reagents were from Sigma-Aldrich.
-
bioRxiv - Cancer Biology 2022Quote: ... Pre-equilibrated Protein G conjugated agarose beads (Roche) were added for 1 hour. ...
-
bioRxiv - Biochemistry 2022Quote: ... and T4 Gene 32 Protein (Gp32; Roche 10972983001) were obtained from commercial ven-dors ...
-
bioRxiv - Cancer Biology 2022Quote: ... Proteins were transferred onto Polyvinylidenfluorid (PVDF) membranes (Roche), blocked with 5% dry milk or 5% bovine serum albumin for 1 hour and incubated with primary antibodies overnight at 4°C ...
-
bioRxiv - Molecular Biology 2022Quote: ... Protein-G agarose beads were manufactured by Roche. All other reagents were from Sigma-Aldrich.
-
bioRxiv - Cell Biology 2023Quote: ... The protein was digested with AspN protease (Roche) at 30°C for 16 hr ...
-
bioRxiv - Plant Biology 2023Quote: ... 50 ul of Protein A-agarose beads (Roche) was then added to the extract before overnight incubation ...
-
bioRxiv - Developmental Biology 2021Quote: For colorimetric in situ hybridization (ISH) Digoxigenin (DIG)-labeled antisense RNA probes were synthesized using SP6 or T7 RNA polymerase (Roche, Basel, Switzerland). Digoxigenin RNA probes were generated from plasmid template (sema3fa - pCR4 ...
-
bioRxiv - Developmental Biology 2021Quote: ... The digoxigenin (DIG)-labeled sense or antisense RNA probe for full-length Emi2 was synthesized with a DIG-RNA-labeling kit (Roche Molecular Biochemicals). No signal was detected with sense probes ...
-
bioRxiv - Developmental Biology 2021Quote: ... The DIG-labeled 3’UTR of Emi2 and cyclin B1 RNA probes were synthesized with a DIG-RNA-labeling kit (Roche Molecular Biochemicals). Two μg of probes was incubated with purified Flag-tagged proteins for 15 min ...
-
bioRxiv - Developmental Biology 2021Quote: ... Revelation of the dig-labeled ISH probe was then performed using an anti-Digoxigenin antibody coupled to POD (11207733910; Roche, 1/400) and revealed using a FITC-tyramide (1/400) ...
-
bioRxiv - Molecular Biology 2022Quote: ... According to the manufacturer’s protocol for Southern blot hybridization and detection digoxigenin-labeled hybridization probes (DIG DNA Labeling and Detection Kit, Roche Applied Science, 11175033910) were used ...
-
bioRxiv - Developmental Biology 2022Quote: ... sections were blocked with 10% HI-serum for 2 h and incubated overnight at 4°C with alkaline phosphatase-labeled sheep anti-dig antibody (11093274910, Roche; 1:2500). After washing ...
-
bioRxiv - Genetics 2019Quote: ... Partial f5 cDNA fragments were amplified to synthesize sense and antisense digoxin-labeled RNA riboprobes (DIG RNA labeling kit, Roche, Basel, Switzerland) (Table 1) ...
-
bioRxiv - Developmental Biology 2019Quote: ... The extracted PCR product was then used as a template for digoxigenin labeled cRNA probe transcription with T3 (anti-sense) and T7 (sense) RNA polymerases (Roche, Mannheim, Germany). The cRNA probes were purified using LiCI/ethanol precipitation and re-suspended in nuclease-free water before storage at −80 °C.
-
bioRxiv - Neuroscience 2020Quote: ... Anti-sense riboprobes against target genes were synthesized from 5 µg linearized plasmids using digoxigenin-(DIG) or fluorescein-labeled uridylyltransferase (UTP) (#11685619910, #11277073910, Roche, Mannheim, Germany) and the MAXIscript in vitro Transcription Kit (#AM1312 ...