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Citations for Roche :
751 - 775 of 775 citations for Palmitic acid U 13C16 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: ... 3 μL of each primer at 10 μM and 0.5 μL of 1 U/μL Kapa HiFi HotStart DNA polymerase (Kapa Biosystems, Inc, A Roche Company) for a total volume of 25 μL ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclear pellet was rinsed with 500 µL of resuspension buffer (20 mM Tris pH 8, 75 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 0.125 mM PMSF, 50 % glycerol, 1x Roche complete, 20 U/mL RNase Out) and centrifuged at 4000 xg at 4°C for 3 min ...
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bioRxiv - Molecular Biology 2024Quote: ... 300 mM NaCl, 7.5 mM MgCl2, 0.25 mM EDTA, 1 mM DTT, 1 M Urea, 1 % NP-40, 1x Roche complete, 20 U/mL RNase Out), pipetting up and down 30 times ...
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bioRxiv - Molecular Biology 2024Quote: ... 8 μCi of α-32P-[ATP] and α-32P-[UTP] and 20 U of T7 RNA Polymerase or SP6 RNA Polymerase (Roche Applied Sicences). The reaction was incubated for 1 h at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... were cultured in RPMI 1640 supplemented with 20% FBS and 10 U/mL recombinant human interleukin-2 (hIL-2; Roche Diagnostics GmbH, Mannheim, Germany).
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bioRxiv - Molecular Biology 2024Quote: Cells were resuspended in 5 mL of lysis buffer (30 mM Tris pH 7.5, 300 mM NaCl, +400 U DNAse I, and a tablet of Complete EDTA-free protease inhibitor cocktail, Roche, per 100 mL of buffer) per gram of cells ...
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bioRxiv - Developmental Biology 2022Quote: ... The samples were then washed with 0.1% PBST and incubated in 1% blocking buffer (1% blocking reagent [Roche, 11175041910] in Maleic acid) for 1 h ...
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bioRxiv - Immunology 2021Quote: ... was incubated with 50μl MTT (sodium 3′-[1-(phenylaminocarbonyl)-3,4-tetrazolium]-bis (4-methoxy-6-nitro) benzene sulfonic acid hydrate) labeling reagent (Roche Life Science, USA) to each well ...
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bioRxiv - Microbiology 2020Quote: ... Total nucleic acid was extracted from the lysate using the Roche MagNA Pure Compact Nucleic Acid Isolation Kit I and the MagNA Pure Compact instrument (Roche, Indianapolis, Indiana). Dual-indexed sequencing libraries were prepared from the lysates using Nextera XT DNA Library Preparation Kit (Illumina FC-131-1096) ...
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bioRxiv - Microbiology 2024Quote: ... Viral RNA isolation was performed using the MagNA Pure LC system (Total nucleic acid isolation kit, Roche Molecular System, Laval, QC, Canada) or the EZ2 Connect system (EZ1&2 Virus Mini Kit v2.0 ...
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bioRxiv - Cell Biology 2024Quote: ... mRNA levels were determined using gene-specific primers by SYBR green nucleic acid labeling (Selleckchem PCR Mastermix, #B21202) in a Lightcycler 480 (Roche, Indianapolis, IN). Fold change was calculated using the delta delta C(t ...
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bioRxiv - Microbiology 2022Quote: ... Samples were transferred to a high pure extender assembly from the High Pure Viral Nucleic Acid Large Volume kit (Roche, Mannheim, Germany) and centrifuged for 8 min with 1,500 rpm at room temperature ...
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bioRxiv - Synthetic Biology 2022Quote: ... 140 mM NaCl, 1mM ethylenediaminetetraacetic acid, 1% Triton X-100, 0.1% Na-Deoxycholate, 1 mM phenylmethylsulfonyl fluoride, 200 µL Roche cOmplete protease inhibitors). 0.5 mm diameter glass beads were added to 1 mm below the meniscus ...
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bioRxiv - Immunology 2024Quote: ... dissolved in Milli-Q water at the manufacturer specified concentration and supplemented with 0.1% fatty acid free bovine serum albumin (Roche Diagnostics, Mannheim, Germany), 1X Glutamax ...
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bioRxiv - Neuroscience 2024Quote: ... 0.1% Triton X100 with glacial acetic acid added until pH 4.0 was achieved] containing a protease inhibitor cocktail (cOmplete mini tablets, Roche cat. no. 11836153001). Homogenates were prepared via probe sonication for 5-7 sec ...
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bioRxiv - Cell Biology 2022Quote: ... 0.1 mM CaCl2, 5 mM EGTA, 50 mM sucrose, 0.1% Triton X-100, 1/2 tablet of cOmplete inhibitor [Roche], and 2.5 U/mL Benzonase [Novagen]), lysed by sonication ...
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bioRxiv - Cell Biology 2020Quote: ... 1 µL egg extract was diluted in 16.5 µL reaction buffer (80 mM β-glycerophosphate, 20 mM EGTA, 15 mM MgCl2, 10 uM okadaic acid, 1x protease inhibitor (Roche, cOmplete #11873580001), 100 µM ATP ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated human MIF was produced using D-biotinoyl- ε -aminocaproic acid-N-hydroxy-succinimide ester (Biotin-7-NHS) with the Biotin Protein Labeling Kit from Roche (Mannheim, Germany). Alternatively ...
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bioRxiv - Genomics 2023Quote: DNA was purified from 200μL Aptima tube specimens using the MagNA Pure Total Nucleic Acid Isolation Kit I (Roche Applied Science, Indianapolis, IN) external lysis protocol on the automated MagNA Pure 24 Instrument and 50µL elution ...
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bioRxiv - Biophysics 2024Quote: ... the lysate powder was resuspended in 100-200 mL of room temperature eIF3 Lysis Buffer supplemented with 2 ethylenediamine tetraacetic acid (EDTA)-free protease inhibitor tablets (Roche, Catalog Number 04693132001), 20 mM imidazole ...
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bioRxiv - Microbiology 2024Quote: ... Pellets were resuspended in a protoplast buffer (100 mM Tris-HCl pH 7.5, 2 mM MgCl2, 1M Sucrose, 6 μg/mL of DNAse/ RNAse, 1x protein inhibitor Roche, 800 U mutanolysin, 8 mg/ml lysozyme) and incubated at 30°C for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... the pocks positive for both BleCherry and GFP signals (pocks containing the recombinant green/red virus) were isolated for DNA extraction using High Pure Viral Nucleic Acid Kit (Roche Diagnostics GmbH, Mannheim, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... hoffmanii kept in RNAlater buffer were transported to DZIF where total nucleic acids (RNA) were extracted using the MagNA Pure 96 DNA and Viral NA Large Volume Kit (Roche Diagnostics, Indianapolis, IN, USA) in a Magna Pure 96 Instrument (Roche Diagnostics) ...
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bioRxiv - Microbiology 2024Quote: ... 1 mM EDTA, 1 mM DTT, 1 mM MgCl2, 1% digitonin, 6 μg/mL of DNAse/RNAse, 1x protein inhibitor Roche, 8 U mutanolysin, 8 mg/mL lysozyme) and incubated at 37°C for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... DNA isolation was performed with a MagNA Pure LC total Nucleic Acid Isolation kit in the MagNA Pure® system (Roche Diagnostics, Almere, the Netherlands). Samples were tested with a Chlamydiaceae PCR targeting the 23S rRNA and C ...