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Citations for Roche :
751 - 800 of 6909 citations for 7 Bromo 2 tert butyl imidazo 1 2 a pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... Total RNA (~ 2 μg) was treated with DNase I (Roche Applied Sciences) and used for cDNA synthesis using SuperScript IV Reverse Transcriptase (Life Technologies-Invitrogen) ...
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bioRxiv - Cancer Biology 2019Quote: ... antigen retrieval performed using citrate (Roche Cell Conditioning 2 Cat#950-123) pre-treatment for 30 minutes ...
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bioRxiv - Pathology 2021Quote: ... 2 mg/ml collagenase IV and 0.2 mg/ml DNase I (Roche) in RPMI 1640 for 40 min at 37℃ with 150 rpm shaking ...
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bioRxiv - Developmental Biology 2019Quote: ... Slides were blocked for 30 min in ISH-blocking solution (2% Roche blocking reagent ...
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bioRxiv - Physiology 2020Quote: ... dissociated by adding 500 μl of 2 mg/ml collagenase P (Roche) dissolved in HBSS and incubated for 15 min at 37 °C ...
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bioRxiv - Bioengineering 2021Quote: ... 700 µl of T cell media + 30 U/ml rhIL-2 (Roche) was added to each well ...
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bioRxiv - Biochemistry 2021Quote: ... 2% Triton X-100) supplemented with protease inhibitors and phosphatase inhibitors (Roche).
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bioRxiv - Biochemistry 2021Quote: ... 2 mM EDTA and cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche)) ...
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bioRxiv - Neuroscience 2021Quote: ... Embryos were rinsed and blocked in TNB solution (2% blocking solution (Roche) in TNT ...
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bioRxiv - Immunology 2021Quote: ... Cells were then PCR amplified in KAPA HiFi 2× mix (Kapa Biosystems) with barcoding primers ...
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bioRxiv - Immunology 2021Quote: ... then chopped and incubated in collagenase P (2 mg/mL; Roche Diagnostics) and DNAase in 10% FBS complete RPMI media at 37°C for 120 min ...
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bioRxiv - Cell Biology 2021Quote: ... The cartilage pieces were incubated with 2 mg/ml pronase solution (Roche) for 90 min at 37 °C and digested overnight at 37 °C in 1.5 mg/ml collagenase B solution (Roche) ...
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bioRxiv - Cell Biology 2022Quote: ... 2 mM phenylmethylsulfonyl fluoride (PMSF)] supplemented with cOmplete Protease Inhibitor Cocktail (Roche) and PhosSTOP (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... and 2 μl of 10 mg/ml DNase (Roche, catalog no. 10104159001). Lymph node tissues were incubated at 37°C for 30 min and filtered through a 70 μm cell strainer ...
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bioRxiv - Neuroscience 2022Quote: ... 10% glycerol) supplemented with 2 mM Na3VO4 and cOmplete inhibitor mix (Roche), centrifuged (4°C ...
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bioRxiv - Genomics 2023Quote: ... 5 mM 2-mercaptoethanol and cOmplete Protease Inhibitor Cocktail (Roche, no. 11697498001). The cell suspension was subjected to sonication (Qsonica ...
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bioRxiv - Neuroscience 2023Quote: ... and 2 mM DTT) supplied with EDTA-free protease inhibitor cocktail (Roche). The lysis was performed using homogenizer EmulsiFlex-C3 (Avestin) ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei were counterstained with 4’,6-Diamidine-2’-phenylindole-dihydrochloride (DAPI; Roche).
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bioRxiv - Microbiology 2023Quote: ... and 2 μl of 10 mg/ml DNase (Roche, catalog no. 10104159001) and incubated at 37°C for 30 min ...
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bioRxiv - Immunology 2023Quote: ... 50μM beta-mercaptoethanol and 10 ng/ml recombinant human IL-2 (Roche). Both human and murine CD4+ T cells were cultured for 46 hrs under humidified conditions at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... Neurons were lysed in 2% Triton X-100 containing protease inhibitors (Roche). A BCA assay (Pierce ...
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bioRxiv - Synthetic Biology 2023Quote: ... The supernatant was applied to 2 mL of cOmplete Ni2+-agarose (Roche) prewashed with Buffer B (20 mM NaPi ...
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bioRxiv - Neuroscience 2023Quote: ... The bill-skin was treated with 2 mg/mL collagenase P (Roche) in Krebs solution for 5 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... 10 mM 2-mercaptoethanol] containing one tablet of protease inhibitor cocktail (Roche), which can inhibit a broad spectrum of serine and cysteine proteases ...
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bioRxiv - Synthetic Biology 2024Quote: 2 mg of the isolated RNA was digested with DNAse I (Roche) and cDNA was synthesized using the GoScript reverse transcription kit A5001 (Promega) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 mM BME and one tablet of EDTA-free protease inhibitors (Roche)) ...
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bioRxiv - Molecular Biology 2023Quote: ... nuclei were stained using 4’,6-diamidino-2-phenylindole (DAPI, Roche Diagnostics) according to the manufacturer’s instructions.
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bioRxiv - Systems Biology 2023Quote: ... a total of 121 μL of 2× Kapa HiFi HotStart ReadyMix (Roche), 9.68 μL of PCR_PF (10 μM) ...
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bioRxiv - Neuroscience 2024Quote: ... 2% sodium dodecyl sulfate (SDS)] supplemented with complete protease inhibitor cocktail (Roche Diagnostics Corp. ...
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bioRxiv - Genomics 2024Quote: ... Each PCR reaction contained 26.25Lμl 2× KAPA HiFi HotStart master mix (Roche), 2.5Lμl of 10LμM TruSeq RPIX primer (Illumina) ...
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bioRxiv - Microbiology 2024Quote: ... 10% glycerol and 2 protease inhibitor cocktail tablets (cOmplete, EDTA free, Roche)) ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 μL of 2× Sybr Green (LightCycler 96, Roche Molecular Systems, Inc.), 200 nM of each primer ...
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bioRxiv - Cell Biology 2023Quote: ... and 2 μl of RNase-free DNase I (20 μ/ml, Roche) were added to the washed antibody beads and were precipitated ON rotating at 4°C ...
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bioRxiv - Microbiology 2019Quote: Mature cysts were washed in PBS and suspended in lysis buffer (10 mM HEPES, 25 mM KCl, 1 mM CaCl2, 10 mM MgCl2, 2% CHAPS, and 1X Roche protease inhibitor) (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... endogenous peroxidase activity was blocked by adding H2O2 and incubating for 10 minutes followed by 2 more washes with PBS-tween and blocking by PBSK [PBS containing 1% bovine serum albumin (Roche, Mannheim, Germany) and 0.2% skim milk (Easis ...
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bioRxiv - Cancer Biology 2021Quote: ... tumor tissues up to 1 g were mechanically minced and digested in 2 ml RPMI1640 with 200 µg/ml Liberase TL (Roche by MilliporeSigma), 200 µg/ml Liberase DL (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... washed once with PBS and suspended in Homogenization Buffer (HB: 250 mM sucrose, Sigma-Aldrich; 10 mM HEPES pH 7.4, Sigma-Aldrich; 1 mM EDTA, Sigma-Aldrich, 2 mM PMSF, Roche). Homogenization was performed by a single freeze/thaw cycle ...
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bioRxiv - Plant Biology 2021Quote: ... The final clean nuclear pellet was resuspended in 1 mL nuclear lysis buffer (20 mM Tris pH 8, 2 mM EDTA, 0.1% SDS, 1 mM PMSF, 1X Roche protease inhibitor cocktail) and sonicated on the Covaris E220 (150W peak power ...
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bioRxiv - Developmental Biology 2022Quote: ... sections were blocked with 10% HI-serum for 2 h and incubated overnight at 4°C with alkaline phosphatase-labeled sheep anti-dig antibody (11093274910, Roche; 1:2500). After washing ...
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bioRxiv - Genetics 2021Quote: ... and cell pellets were then resuspended in 5ml of buffer 1 (10 mM Tris-HCl at pH 7.5, 2 mM MgCl2, 3 mM CaCl2, Roche Complete protease inhibitor), incubated for 20 min at 4°C followed by a centrifugation step ...
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bioRxiv - Genetics 2021Quote: ... 30 million equivalent cell pellets were then resuspended in 5ml of buffer 1 (10 mM Tris-HCl at pH 7.5, 2 mM MgCl2, 3 mM CaCl2, Roche Complete protease inhibitor), incubated for 20 min at 4°C and collected cells by a centrifugation step ...
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bioRxiv - Genetics 2020Quote: Cell pellets from THP-1 cells were suspended on ice-cold lysis buffer (PBS containing 2% SDS and complete protease inhibitor cocktail (Roche, catalog 11697498001)) ...
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bioRxiv - Microbiology 2022Quote: ... was used at a 1:1000 dilution to detect specific anti–SARS-2 immunoreactivity using the Discovery ULTRA automated staining instrument (Roche Tissue Diagnostics) with a Discovery ChromoMap DAB (Ventana Medical Systems ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The left lobe minced into small (~1-2 mm) sections and digested using 0.5 mg/mL collagenase D (Roche Diagnostics, Mannheim, Germany) and 50 U/mL DNase I (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... cover slips were washed with PBS and incubated for 10 min at room temperature with 1 g/mL 4’,6 diamidino-2-phenylindole (DAPI, Sigma Aldrich, Roche, #10236276001) in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... The pellet was resuspended in Buffer B (10 mM Pipes, 50 mM KCl, 5 mM MgCl2, 1 mM DTT, 2 mM PMSF, Roche protease inhibitor) with 1% Triton X-100 and incubated on ice for 1 hour ...
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bioRxiv - Molecular Biology 2024Quote: Beads were then washed twice with Low Salt Wash Buffer (150 mM NaCl, 0.1 % SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl pH 8, 1x Roche protease inhibitor mixture), twice with High Salt Wash Buffer (500 mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: Human peripheral blood mononuclear cells isolated from buffy coats were activated in vitro for 1 week with 20 U/mL of IL-2 (Teceleukin; Roche, Nutley, NJ) and 5 μg/mL phytohemagglutinin (Roche ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were lysed by addition of 250 μl lysis buffer (50 mM Tris/HCl, 500 mM NaCl, 2% w/v SDS, add freshly 1 mM DTT, 1x Roche inhibitor cocktail) followed by incubation on ice for 10 min ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was sonicated in homogenization buffer (1% SDS, 50 mM Tris pH 7.4, 2 mM EDTA, protease and phosphatase inhibitors (Roche 11873580001; Roche 04906837001), heated at 95° C for 5 minutes ...