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Citations for Roche :
751 - 800 of 6989 citations for 1 Benzyl 3 Tert Butyldimethylsilyl Oxy Methyl Piperidin 4 One since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... Illumina adapters and indices were added in the same one-step PCR reaction using the KAPA HiFi HotStart ReadyMix (Roche). The PCR product was purified using the QIAQuick PCR Purification Kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... One to five ng of DNA was analyzed by qPCR using 300 nM primers and SYBRgreen Master mix (Roche AG) using a QuantStudio 7 Flex Real Time PCR System (Applied Biosystems) ...
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bioRxiv - Biochemistry 2021Quote: ... 0.1 mg/mL lysozyme (∼20,000 U/mg; Carl Roth) and one tablet cOmplete™ ULTRA EDTA-free protease inhibitor tablet (Roche). Cells were lysed by homogenization using an EmulsiFlexC5 (Avestin Inc. ...
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bioRxiv - Immunology 2020Quote: ... cells were cotransfected with both the reporter gene and one of the different putative transcription factors using X-tremeGENE 9 (Roche) following the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2020Quote: ... embryos were washed in MABT buffer for one hour and NTMT buffer for 10 min and incubated in NBT/BCIP (Roche) for the coloring reaction ...
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bioRxiv - Microbiology 2020Quote: ... thus one negative control is placed after every 5 samples on a Light cycler 480 multiwell plates 96-well plate (Roche).
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bioRxiv - Microbiology 2021Quote: ... The cell pellet was resuspended in 10 ml PdeL purification buffer and one tablet of c0mplete mini EDTA-free protease inhibitor (Roche) and a spatula tip of DNase I (AppliChem ...
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bioRxiv - Genetics 2020Quote: ... One short-insert WGS library was constructed using the Kapa Hyper Prep Kit (KR0961) from Kapa Biosystems (Woburn, MA, USA) with 150 ng of sheared DNA as input following standard manufacturer’s protocol with 10 cycles of PCR ...
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bioRxiv - Molecular Biology 2020Quote: ... Only one sample was obtained per patient: one portion of the sample was extracted using the High Pure Viral RNA Kit (Roche), and another portion of the same sample was extracted using the acid pH method ...
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bioRxiv - Microbiology 2021Quote: ... before being resuspended in 1 mL of lysis buffer (100 mM NaCl, 5% glycerol, 10 mM Tris, pH 8; one cOmplete Mini EDTA-free protease inhibitor pellet (Roche) per 15 mL) ...
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bioRxiv - Physiology 2022Quote: ... Fasting blood glucose was assessed by collecting blood from mice via tail puncture and using the one-touch Accu-Chek Aviva glucometer (Roche). Afterwards ...
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bioRxiv - Microbiology 2024Quote: ... RT-qPCR was performed using the Luna Universal One-Step RT-qPCR Kit (Biolaps) according to the manufacturer protocol on a LightCycler real-time PCR thermocycler (Roche). The analysis of the infection rate based on PCR data was done with the binGroup package [42] in R software.
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bioRxiv - Neuroscience 2023Quote: ... with one modification to Homogenization Buffer preparation required in which cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche, Basel, Switzerland) and Phosphatase Inhibitor Cocktail 2 (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... and the pellet was resuspended in 5 volumes of the lysis buffer (buffer A including one EDTA-free protease inhibitor tablet (Roche); buffer A ...
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bioRxiv - Systems Biology 2023Quote: ... Coverslips were washed with PBS three times for five minutes and blocked in a humid chamber for one hour at room temperature in blocking solution (1x Western Blocking reagent [Roche, 11921673001] diluted in PBS and supplemented with 0.3% Triton X-100) ...
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bioRxiv - Biophysics 2023Quote: ... Pellets were resuspended in 7 ml of buffer A supplemented with the addition of 200 μl of protease inhibitor cocktail (one tablet (Roche), dissolved in 1 ml buffer A) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were grown under induction for one generation and homogenised using bead beater (MPBio) in lysis buffer with protease inhibitor cocktail (Roche): 12 cycles of 7 m/sec ...
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Bni5 tethers myosin-II to septins to enhance retrograde actin flow and the robustness of cytokinesisbioRxiv - Cell Biology 2023Quote: ... Cells were then lysed by sonication 6 times for 15 sec each in one of the following buffers each containing the Protease Inhibitors Cocktail tablets (Roche) and 1 mg/mL lysozyme (L6876 ...
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bioRxiv - Microbiology 2023Quote: ... To prevent adherence of cells to one another by DNA leakage the suspension of cells was incubated with 600 Units of DNAse I (Roche) (30 mins ...
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bioRxiv - Neuroscience 2024Quote: ... sciatic nerves (4 sciatic nerves were pooled for one preparation in order to generate enough material for transcriptomic analysis) and brains were enzymatically digested with Liberase DL (Roche) at 143μg.mL-1 and DNAse I (Sigma ...
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bioRxiv - Biochemistry 2020Quote: ... blocked in 3% BSA and probed with anti-HA (clone 12CA5, Roche), anti-cMyc (clone 9E10 ...
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bioRxiv - Neuroscience 2020Quote: ... Following resuspension in (3 ml) homogenization medium containing protease inhibitor (Roche Diagnostics), the cells were cracked open using a ball bearing homogenizer with a 0.2507-inch bore and 0.2496-inch diameter ball ...
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bioRxiv - Systems Biology 2020Quote: ... The tissue was digested with Liberase Blendzyme 3 recombinant collagenase (Roche Diagnostics) according to the manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The cartilage was then incubated in 3 mg/mL Collagenase D (Roche) in DMEM solution for two 45 minute periods and transferred to 0.5 mg/mL Collagenase D in DMEM solution supplemented with 3% Liberase TL (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... Nuclei lysates (in Nuclear Buffer Lysis 3, supplemented with protease (Roche, 11697498001) and phosphatase inhibitors (1 mM Na3O4V (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... and 3 µl 10 mM dNTP (KAPA HiFi Kits from Roche, KK2502) were added to the annealed oligos ...
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bioRxiv - Microbiology 2024Quote: ... 3 mM MgCl2) supplemented with complete Protease inhibitor cocktail tablets (Roche 11873580001). The lysates were incubated on ice for 1 hour and centrifugated at 20,000 x g for 15 minutes at 4°C to pellet the nuclear fraction ...
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bioRxiv - Neuroscience 2020Quote: ... slices were incubated with 4-Nitroblue tetrazolium chloride (NBT, Roche) and 5-bromo-4-chloro-3-indolyl-phosphate (BCIP ...
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bioRxiv - Biophysics 2020Quote: ... 4 mM digitonin and EDTA-free protease inhibitor tablets (Roche). Insoluble material was removed by ultracentrifugation at 100,000 g for 30 min ...
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bioRxiv - Physiology 2020Quote: ... 4 µL of 0.25 µg/µL trypsin (Roche, sequencing grade) was added to each sample and left overnight at 37 °C.
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bioRxiv - Molecular Biology 2020Quote: ... and then with DAPI (4′,6-Diamidino-2-phenylindole, Roche) for 15 min ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 ul of 20 mg/ml proteinase K (Roche 3115801) was added ...
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bioRxiv - Neuroscience 2024Quote: ... counterstained using the Red Counterstain II kit (4 min, Roche), removed from the automated system and rinsed with distilled water ...
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bioRxiv - Plant Biology 2024Quote: ... mixed with 4 μL of 25-fold protease inhibitor (Roche), and frozen at 80°C ...
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bioRxiv - Systems Biology 2024Quote: ... 4 ul of 20 mg/ml proteinase K (Roche 3115836001) was added to the eluates and inputs and incubated at 50°C for 2 hours to cleave away proteins ...
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bioRxiv - Cell Biology 2024Quote: ... and 4 cOmplete EDTA-free protease inhibitor cocktail tablets (Roche) per 500 mL ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and then treated with 4 μg/mL proteinase K (Roche) before pre- hybridization in 66% formamide and incubation with a mixture of the STBD1 probe with either LC3A ...
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bioRxiv - Microbiology 2020Quote: ... 50 mM Tris-HCl (pH 8.0) and protease inhibitor (one tablet of Roche cOmplete™ mini per 10 ml lysis buffer) [22] ...
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bioRxiv - Developmental Biology 2022Quote: ... 50mM Tris-HCl [pH 7.2], 250mM NaCl, 2mM EDTA, phosphatase inhibitor mixture I and II [Calbiochem] and one tablet of Minicomplete [Roche] per 10ml) on ice ...
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bioRxiv - Physiology 2021Quote: ... at time zero (T0) peripheral glucose level was measured at the tail using a One-Touch ultra-glucometer (AccuChek Sensor, Roche Diagnostics) and at T15 ...
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bioRxiv - Microbiology 2021Quote: ... 150 mM NaCl, 20 mM Imidazole pH 8.0, one tablet of EDTA-free protease inhibitor [Roche; Product # COEDTAF-RO], DNaseI [Roche; Product #11284932001]) and lysed and centrifuged for 30 minutes at 18,000 x g at 4 °C ...
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bioRxiv - Physiology 2024Quote: Blood glucose levels were measured immediately in one drop of blood after the heart puncture with the Blood Glucose System Accu-Chek Performa (Roche Diagnostics).
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bioRxiv - Immunology 2023Quote: ... One step real time RT-PCR was performed using 100 ng of total RNA and KAPA SYBR FAST One-Step qRT-PCR Kits (Kapa Biosystems) according to the directions provided by the manufacturer ...
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bioRxiv - Biochemistry 2023Quote: ... 4 mM sodium dithionite) supplemented with 0.2 mg/mL bovine pancreatic deoxyribonuclease I and one cOmplete EDTA-free protease inhibitor tablet (Roche, Basel, Switzerland). Subsequently ...
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bioRxiv - Microbiology 2023Quote: ... The cell pellet was resuspended in chilled lysis buffer (Bugbuster) containing one dissolved cOmplete™ EDTA-free Protease Inhibitor cocktail tablet (Roche). The cell lysates were clarified by centrifugation (11,000 × g ...
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bioRxiv - Microbiology 2023Quote: ... bacteria were centrifuged and the pellet was resuspended in Resuspension Buffer (25 mM Tris HCl pH 7.5, 250 mM NaCl) supplemented with one tablet of cOmplete protease inhibitor (Roche, Cat # 04693159001). Cells were then lyzed using a high-pressure Emulsiflex C5 homogenizer (Avestin) ...
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bioRxiv - Microbiology 2024Quote: ... 10 mM imidazole) supplemented with DNase and one cOmplete™ EDTA-free protease inhibitor cocktail tablet (Roche #11 873 580 001). Cells were lysed using a French press (2x ...
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bioRxiv - Developmental Biology 2021Quote: ... One microgram of total RNA was reverse transcribed to cDNA using a Transcriptor First Strand cDNA Synthesis Kit (Roche Diagnostics, Tokyo Japan) using an anchored-oligo(dT)18 primer and random hexamer primer ...
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bioRxiv - Cell Biology 2021Quote: ... of these dilutions were used to prepare the reaction mixes for the real-time qRT-PCR using the KAPA SYBR FAST One-Step Universal kit (Roche, Basel, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... One microgram of the total RNA was reverse-transcribed using the Transcriptor First Strand cDNA Synthesis Kit (Roche Diagnostics, Florham Park, NJ). qPCR was performed using iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories ...