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Citations for Roche :
7901 - 7950 of 8781 citations for 6H Imidazo 4 5 e 2 1 3 benzothiadiazole 7 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Triton X-100 in PBS for 20 min and followed by 1 hr DNAse (1,000 U/ml; #04536282001, Roche) treatment at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... Pelleted cells were resuspended in Lysis buffer (50mM Tris pH 7.4, 150mM NaCl, 5mM MgCl2 1% Triton X-100, protease inhibitors (Roche Complete Inhibitor cocktail ...
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bioRxiv - Molecular Biology 2024Quote: ... and resuspended in 300 µL cold nuclear lysis buffer (1% SDS, 10 mM EDTA, 50 mM tris-HCl pH8.0, 1x protease/phosphatase inhibitor, Roche) and lysed (10 min on ice) ...
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bioRxiv - Molecular Biology 2024Quote: ... and resuspended in 50 µL cold nuclear lysis buffer (1% SDS, 10 mM EDTA, 50 mM tris-HCl pH8.0, 100U Ribolock inhibitor, Thermo, 1x protease/phosphatase inhibitor, Roche) and lysed (10 min ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed twice with DPBS, resuspended in lysis buffer (1% NP40 in PBS, pH 7.4 with protease inhibitor cocktail (Roche), and 2.5% v/v RNase inhibitor) ...
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bioRxiv - Biochemistry 2024Quote: Homogenized lungs were 10-fold diluted starting 1:10 in infection media (DMEM containing 0.1% BSA (fraction V; Roche), 10 mM HEPES (Corning) ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.1 % SDS, 150 mM NaCl, 10 mM EDTA, 20 mM Tris-HCl pH7.5 and complete protease inhibitor mix, Roche) was used to prepare protein lysates ...
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bioRxiv - Biochemistry 2023Quote: ... the membrane was incubated with 1 nM DIG (Digoxigenin)-labeled Telomere probes in Hybridization solution (DIG Easy Hyb, Roche) at 42°C ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Hippocampus was then sliced with a scalpel and digested for 1 h at room temperature in HBSS supplemented with 0.5 mg/ml collagenase D (Roche) and 10 µg/ml DNase I (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2024Quote: ... pooled and resuspended in ice-cold lysis buffer (150 mM NaCl, 50 mM Tris pH 8.0, 1 × cOmplete EDTA-free protease inhibitor (Roche), 1 mg/mL DNase and lysozyme ...
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bioRxiv - Biochemistry 2024Quote: ... Frozen cell pellets were lysed in immunoprecipitation lysis buffer (50 mM Tris [pH = 7.5], 150 mM NaCl, 1% IGEPAL, 10% Glycerol) with cOmplete Protease Inhibitor Cocktail (Roche) and PhosSTOP Phosphatase Inhibitor Cocktail (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... cells were detached using cold PBS and lysed in 350 µl lysis buffer (50 mM Tris pH 8.0, 150 mM NaCl, 1% Triton X-100) containing protease inhibitor (Roche) for at least 20 min on ice ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were washed twice in ice-cold PBS and resuspended in 600 uL NLB (1xPBS, 300 mM NaCl, 1% TritonX-100, 0.1% Tween-20) freshly supplemented with 1x Complete protease inhibitors (Roche), and 1x PhosSTOP (4906845001 ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK cells were lysed in 1xPBS pH 7.4 containing Protease Inhibitor Cocktail tablet (1 tablet/10 mL) (Roche Diagnostics) and separated in 4–16% gradient Native pre-cast gel (SERVA Electrophoresis GmbH) ...
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bioRxiv - Neuroscience 2023Quote: ... The heads were crushed on ice using a pestle in 0.5% NP-40 lysis buffer (50 mM Tris, 150 mM NaCl, 10% glycerol, 1 mM EDTA, pH 7.5) supplemented with protease inhibitors (Roche). The resulting lysates were clarified by centrifugation and the supernatants were collected ...
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bioRxiv - Molecular Biology 2024Quote: ZNF274 expression was induced with 1µg/ml doxycycline and verified via western blot with an anti-HA antibody (1:1000, ref.12013819001, Roche) and anti-beta actin antibody (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... then transferred for 48 hrs in a solution of mouse anti-synaptophysin (1:20, Roche Applied Science, Indianapolis, IN). Sections were then washed in PBS-t 3x10 min ...
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bioRxiv - Molecular Biology 2024Quote: Cells were harvested and protein was extracted using lysis buffer (20mM HEPES pH7.4, 2mM MgCl2, 40mM NaCl and 1% Triton-X) containing complete mini EDTA protease inhibitor (Roche) at 1 in 50 concentration ...
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bioRxiv - Molecular Biology 2024Quote: ... The crude nuclear pellet was resuspended in nuclear lysis buffer (50 mM Tris-HCl, pH 8.0, 10 mM EDTA, 1% SDS, and Complete protease inhibitor; Roche) and sonicated with a Branson sonifier (VWR ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cell viability was determined by using the WST-1 assay according to the manufacturer’s instructions (Roche Diagnostics, Mannheim, Germany). After removing the cell supernatants for cytotoxicity measurements ...
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bioRxiv - Genetics 2024Quote: Tissues were homogenized in 500uL RIPA buffer (10% sucrose, 1% SDS, 5mM HEPES pH 7.9, 10mM sodium butyrate in MilliQ water supplemented by protease inhibitor [Roche 04693124001] ...
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bioRxiv - Developmental Biology 2024Quote: ... ovaries were crosslinked in 250 µL YSS buffer (50 mM pH 8.0 Tris HCl, 50 mM NaCl, 0.1% NP40, 1 U/mL of RNAsin (Roche, 3335402001), protease inhibitor (Thermo Scientific ...
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bioRxiv - Microbiology 2024Quote: ... gondii RH tachyzoites lysed in NP-40 lysis buffer (1% NP-40, 150 mM NaCl, 50 mM Tris [pH 8.0]) supplemented with complete protease inhibitor (Roche). The insoluble material was removed from the lysate by centrifugation at 10,000 x g for 20 minutes ...
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bioRxiv - Molecular Biology 2024Quote: Antibodies used for western blots presented in this work were as follows: anti-HA monoclonal antibody (mAb) 3F10 (diluted 1:2,000) (Roche); mouse anti-GAPDH mAb (1:20,000).
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bioRxiv - Microbiology 2023Quote: ... and 0.1% Triton X-100 supplemented with 1 mM PMSF and one Complete Protease Inhibitor tablet (EDTA-free; Roche), and then centrifuged at 13,000 × g for 10 min ...
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bioRxiv - Biochemistry 2023Quote: ... Bacterial pellets were harvested by centrifugation and resuspended in 50 mL of lysis buffer (20 mM Tris pH 7.5, 500 mM NaCl) to which 1 protease inhibitor tablet was added (Roche). Bacterial cells were lysed by sonication with 10 s pulses for 10 min and cell debris was removed by centrifugation at 25,000 x g for 40 min and cleared cell lysate was filtered through 0.45 μm filters ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5% Nonidet P-40) supplemented with cOmplete Mini EDTA-free protease inhibitor cocktail tablets (1 tablet/10ml) (11836170001, Roche) for 1 h at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Slides were subsequently washed with PBS three times and nuclei were counterstained with DAPI (2.5 μg ml−1, Roche) before mounting with Prolong Gold (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: Single cell suspensions were obtained from dissected muscles mechanically disrupted and enzymatically digested at 37 °C for 1h using DMEM 1% P/S medium containing 0.2% collagenase B (Roche) and Calcium dichloride (CaCl2 ...
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bioRxiv - Cell Biology 2023Quote: ... Mixed-stage transgenic worms expressing ectopic GFP::EGO-1 were collected and resuspended in equal volumes of 2x lysis buffer (50 mM Tris-HCl [pH 8.0], 300 mM NaCl, 10% glycerol, 1% Triton X-100, Roche ®cOmplete EDTA-Free Protease Inhibitor Cocktail ...
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bioRxiv - Immunology 2023Quote: ... euthanized mice were perfused with PBS and the left lung lobes digested for 1 h with collagenase D (1.5 mg/ml, Roche) and DNAse A (40 U/ml ...
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bioRxiv - Cell Biology 2023Quote: ... target proteins were detected by using primary antibodies of mouse monoclonal anti-GFP antibody (1: 2000; Roche, Cat. 11814460001), mouse anti-mCherry (1:5000 ...
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bioRxiv - Genomics 2023Quote: ... The cell pellet was resuspended with ice-cold Galbraith’s buffer amended with bovine serum albumin (BSA, 0.05% w/v) and cOmplete protease inhibitor (1×, Roche 11697498001), lysed by repeated pipetting ...
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bioRxiv - Immunology 2023Quote: ... Popliteal lymph nodes were harvested from immunized mice 1 day later and digested with collagenase D (2.5 mg/mL, Roche) in RPMI-1640 medium supplemented with 10% FBS for 30 min at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were lysed by sonication in 50 mL lysis buffer (20 mM Tris pH 8.5, 200 mM NaCl, 10% glycerol, 1 mM PMSF, 2x Roche protease inhibitor tablets ...
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bioRxiv - Synthetic Biology 2023Quote: ... was amplified in a 5ul qPCR reaction using x2 Lightcycler 480 SYBR Green 1 Master mix (Roche Diagnostics 04707516001) according to the manufacturer’s protocol (15sec at 95℃ and 30sec at 60℃ ...
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bioRxiv - Molecular Biology 2023Quote: ... and frozen in 600 µL of ChIP lysis buffer plus with protease inhibitors and 1 mM PMSF (Roche 11836170001) [ChIP lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... Cell pellets were resuspended in 150 ml lysis buffer (50 mM Hepes pH 7.5, 50 mM NaCl, 1 mM MgCl2, benzonase, EDTA-free protease inhibitor cocktails (ROCHE)) at 4°C and loaded 3 times in a CellD disrupter (Constant Systems) ...
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bioRxiv - Plant Biology 2023Quote: ... pH 7.6; 15 mM NaCl; 10% glycerol; 0.5% Tween 20; 10 mM imidazole; 1 mM DTT; 20 mM NaHCO3; Roche protease inhibitors cocktail without EDTA) ...
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bioRxiv - Cell Biology 2023Quote: ... the membranes were blocked using non-fat dry milk and stained with an anti-GFP primary (Roche; 1:1000) and a goat anti-mouse secondary antibody coupled to horseradish peroxidase (HRP ...
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bioRxiv - Molecular Biology 2023Quote: Cell lysates were extracted using Igepal lysis buffer (1% Igepal, 150mM NaCl, 50mM Tris pH 7.4) enriched with protease inhibitor cocktail (Roche). Proteins were analyzed by SDS-PAGE and immunoblotting.
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bioRxiv - Molecular Biology 2023Quote: 3-5 day old males of the indicated genotypes and experimental groups were homogenised in 150µl of homogenisation buffer (0.2%Triton X-100 supplemented with 1 x complete mini EDTA-free protease inhibitor (Roche) in PBS ...
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bioRxiv - Immunology 2023Quote: ... Aortas were manually homogenized and digested for 20 minutes at 37°C in RPMI with 1 mg/mL of collagenase A and collagenase B and 0.1 mg/mL DNAse I (Roche). Kidneys were homogenized using the Miltenyi gentleMACS Dissociator and digested for 20 minutes at 37°C in RPMI with 2 mg/mL collagenase D (Roche ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cells were lysed using 200 μL/well cell lysis buffer (100 mM Tris-HCl pH 8.8, 1 % SDS) and protease inhibitors (cOmplete, Roche) on ice for one h ...
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bioRxiv - Plant Biology 2023Quote: ... 10 mM NaCl, 1% SDS, 0.1 mM dithiothreitol, 20 μM PMSF, 0.5% β-mercaptoethanol, 1X proteinase inhibitor from Roche) and centrifuged for 10 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Pellet was resuspended in high-salt buffer (750 mM NaCl, 10 mM Tris, pH 7.6, 1 mM EDTA, protease inhibitor tablet (Roche)) and lysed by sonication at 60% power using a probe sonicator (Branson sonifier 450 ...
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bioRxiv - Molecular Biology 2023Quote: ... pH 8.0) or Lysis buffer (1 % SDS, 50 mM Tris pH6.8 and 10 % Glycerol) supplemented with protease inhibitor cocktail (Roche) and subsequently quantified using Pierce BCA Protein Assay Kit (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 mM DTT, 0.4% Igepal CA-630, 1 mM MgCl2, 200 µg/ml lysozyme, 10 µg/ml pepstatin A, Roche cOmplete protease inhibitor cocktail ...
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bioRxiv - Microbiology 2023Quote: ... and lysed using NP-40 lysis buffer (50 mM Tris [pH 8.0], 150 mM NaCl, 1% NP-40) supplemented with Complete Protease Inhibitor Cocktail (Roche) for 30 minutes on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... The total RT-qPCR reaction volume was 1.5⍰μL and consisted of 1⍰μL of LightCycler 480 SYBR Green I Master Mix (Roche) containing 0.5⍰μM of PCR primer (Eurogenetec ...