Labshake search
Citations for Roche :
7751 - 7800 of 8217 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... pH 7.2) with 2 mM PMSF and EDTA-free Complete Protease Inhibitors Cocktail tablets (Roche Diagnostics, Mannheim, Germany), and its supernatant was transferred onto 30-60% sucrose gradient ...
-
bioRxiv - Genomics 2020Quote: ... Specimens in May were tested using the Cobas® SARS-CoV-2 RT-PCR assay (Roche Diagnostics, USA). Respiratory samples archived at the Clinical Laboratory ...
-
bioRxiv - Cell Biology 2022Quote: ... Inguinal stromal-vascular fractions (SVF) were isolated from 2-week-old C57BL/6J mice with collagenase D (Roche) digestion and differentiated as described previously34 ...
-
bioRxiv - Microbiology 2022Quote: ... was used to detect SARS-CoV-2 mRNAs and visualized by DIG luminescent detection kit (Roche, Indianapolis, IN), according to the manufacturer’s protocol.
-
bioRxiv - Molecular Biology 2022Quote: ... The nuclear lysates were diluted 2 fold with IP buffer (20mM HEPES, pH 7.9, 0.2M NaCl, complete protease inhibitors (Roche) and then flag-beads (Anti-Flag Magnetic Beads ...
-
bioRxiv - Genetics 2022Quote: ... 50 mM Hepes pH 7.6, 2 mM DTT, 0.1 mM PMSF and supplemented with complete protease inhibitor tablets, Roche) were added dropwise and on a vortex with constant low speed to ensure immediate mixing ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were collected and washed with ice-cold 1X PBS before being resuspended in Sonication Buffer (20 mM Tris, pH 8.0, 2 mM EDTA, 0.5 mM EGTA, 1X Complete Mini EDTA-free Protease Inhibitor Cocktail [Roche 11836170001] ...
-
Atypical epigenetic and small RNA control of transposons in clonally reproducing Spirodela polyrhizabioRxiv - Plant Biology 2024Quote: ... 0.1% NP-40) containing 2 μM MG-132 and cOmpleteTM mini EDTA-free protease inhibitor cocktail (Roche, #04693159001) for 30 minutes at 4 °C ...
-
bioRxiv - Plant Biology 2024Quote: ... The oocytes were homogenized in 2 ml Lysis buffer (Tris-HCl pH 8.0 50 mM, ½ tablet PhosSTOP (Roche), 1x cOmpleteTM EDTA-free proteinase inhibitor cocktail (Sigma-Aldrich) ...
-
bioRxiv - Microbiology 2024Quote: ... Peritoneal cavities were lavaged by injection of 2 mL of sterile PBS containing 1X protease inhibitors (cOmplete, Roche) or stable isotope labeling with amino acids in cell culture (SILAC ...
-
bioRxiv - Genomics 2024Quote: ... Cells were re-suspended in digestion buffer (50 mM Tris-HCl pH7.6, 2 mM CaCl2, 0.2% NP40) containing protease inhibitors (complete EDTA-free, Roche) and warm-up to 37°C for 2min ...
-
bioRxiv - Immunology 2024Quote: ... System performance was assessed by an Fc/2 standard obtained from a commercial mAb preparation (Herceptin; Roche Diagnostics).
-
bioRxiv - Bioengineering 2024Quote: ... the infected lungs were transferred to gentleMACSTM C tubes and homogenized on a gentleMACSTM tissue dissociator (“m_lung_01” program) using HEPES buffer (2 mg/ml collagenase D (Roche) and 80 U/mL DNAse I (Roche) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Screen for virus progeny production was done with SARS-CoV-2 antigen quick-test (Roche, 9901-NCOV-01G) (or CPE in E2T ...
-
bioRxiv - Neuroscience 2022Quote: ... Supernatants were removed and pellets were resuspended in 2% BSA/PBS containing RNase inhibitor (0.2 U/μL, Roche). To enrich astrocyte nuclei ...
-
bioRxiv - Biochemistry 2022Quote: ... 500 mM NaCl, 2 mM TCEP, 20% glycerol, 1.4 μg/mL leupeptin, 1.0 μg/mL pepstatin A and two Roche EDTA-free protease tablet per 5 g cell pellet) ...
-
bioRxiv - Biochemistry 2022Quote: ... 500 mM NaCl, 2 mM TCEP, 20% glycerol, 1.4 μg/mL leupeptin, 1.0 μg/mL pepstatin A and two Roche EDTA-free protease tablet per 5 g cell pellet) ...
-
bioRxiv - Microbiology 2023Quote: ... and 2 mM b-mercaptoethanol (BME)] in the presence of an anti-protease cocktail (Complete EDTA-free, Roche) and 1 μl of benzonase (Merck Millipore ...
-
bioRxiv - Neuroscience 2023Quote: ... 2 ul of each sample were used for library quantification using the KAPA Library Quantification Kit (Roche #KK4923). The rest of the DNA eluate (20ul ...
-
bioRxiv - Developmental Biology 2023Quote: ... then incubated in 2 ml HBSS solution containing 2.5 mg/ml collagenase A (Roche, 11 088 793 001) and 10 mg/mL pancreatin (Sigma ...
-
bioRxiv - Cell Biology 2023Quote: ... primer efficiency was first calculated by amplifying cDNA template with KAPA 2× SYBR Master mix (Roche catalog 07959362001) and quantifying the SYBR intensity using a Roche LightCycler 480 at three different concentrations of cDNA 10-fold apart (1/2 ...
-
bioRxiv - Cell Biology 2023Quote: ... and lysed in lysis buffer (50mM Tris, pH 7.4, 500mM NaCl, 0.4% SDS, 2% Triton-X-100, 1mM DTT, 1x complete protease inhibitor [Roche]). Lysates were sonicated twice for 1 minute at 30% duty cycle at an output level of 4 ...
-
bioRxiv - Genomics 2023Quote: ... Library QC was performed before and after capture in 2 steps: quantification using qPCR (Kapa Biosystems, part #KK4602) and QC using LabChip GX Touch HT Nucleic Acid Analyzer ...
-
Transcriptional Isoforms of NAD+ Kinase regulate oxidative stress resistance and melanoma metastasisbioRxiv - Cancer Biology 2023Quote: ... and 2 cocktails of protease and phosphatase inhibitor (Halt™, Fisher Scientific; Phosstop™-phosphatase inhibitor tablets, Roche). The Pierce Rapid Gold BCA Protein Assay Kit (Thermo Fisher Scientific ...
-
bioRxiv - Genomics 2023Quote: ... Whole genome amplification was performed by addition of 20 μL 2× KAPA HiFi HotStart Uracil+ ReadyMix (Roche KK2801), 0.4 μL 100 μM Nextera P5 index primer and 0.4 μL 100 μM Nextera P7 index primer and incubation at 98°C for 45 s ...
-
bioRxiv - Cell Biology 2023Quote: ... Embryos were incubated for 2-2.5 hours at room temperature with a rat anti-HA antibody (Roche, #11867423001) at a 1:1000 dilution ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... mice (n=2 per group) were randomly assigned to receive either diazepam (synthesized by F. Hoffmann-La Roche) 0.3 ...
-
bioRxiv - Cell Biology 2024Quote: Cultured cells were lysed on ice through repeated pipetting in 2% SDS supplemented with Protease Inhibitor Cocktail (Roche) and PhosStop phosphatase inhibitor (Roche) ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... Blocking was performed in MAB blocking buffer (100 mM Maleic acid, 150 mM NaCl, 2% Blocking reagent [Roche, 1096176] ...
-
bioRxiv - Molecular Biology 2024Quote: ... DNA (2 µl) was added to the mixture with FastStart SYBR Green Master Mix kit (Roche, Basel, Switzerland) and specific primers SP_F 5’-GAC-CAG-TCG-AAC-GCA-CAT-TG-3’ and SP_R 5’-CGG-AGA-GGG-TTG-TTG-TGT-CT-3’ ...
-
bioRxiv - Neuroscience 2024Quote: ... whereas the pellet (insoluble fraction) was resuspended in 2% SDS/TBS supplemented with protease inhibitor cocktail (Roche, Switzerland), 1 mM PMSF and phosphatase inhibitor cocktail 2 and 3 (Sigma-Aldrich ...
-
bioRxiv - Physiology 2024Quote: ... Glomus cells were dissociated using a mixture of collagenase P (2 mg/ml; Roche Applied Science, Indianapolis, IN), DNase (15 μg/ml ...
-
bioRxiv - Immunology 2024Quote: ... Digestion was performed for 30 min at 37⍰C with 2 mg/ml collagenase D (Roche, Meylan, France), 1 mg/ml dispase (Invitrogen ...
-
bioRxiv - Biochemistry 2024Quote: ... 2 µl RNA were incubated for 10 min at room temperature with 0.6 µg baker’s yeast tRNAPhe (Roche), 1 mM MgCl2 and increasing amounts of protein in a volume of 20 µl ...
-
bioRxiv - Cancer Biology 2024Quote: ... Tumor tissue was mechanically dissociated into 2-4mm pieces and then digested in 2.5 mg/mL Liberase and 0.1 mg/mL DNase (Roche) for 1 hour at 37C ...
-
bioRxiv - Cell Biology 2021Quote: ... The pellet was washed in buffer A three times and then homogenized in 400 ul of buffer B (250 mM sucrose, 10 mM Tris HCl [pH 8.0], 10 mM MgCl2, 1 mM EGTA, 1X protease-inhibitor cocktail [11697498001, Roche], 0.1% Triton X-100, and 0.25% NP-40) for 10 min at 4°C ...
-
bioRxiv - Biochemistry 2024Quote: ... 150 mM NaCl, protease inhibitors (10 μg/ml leupeptin, 20 μg/ml aprotinin, EDTA-free Complete protease inhibitor tablet (Roche, 1 tablet per 50 mL of buffer), 1 mM PMSF ...
-
mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... Coralville, Iowa, USA) and 0.25 µl reverse primer (5 µM, IDT) and was analyzed on a LC480 instrument (Roche, Basel, Switzerland).
-
bioRxiv - Cancer Biology 2021Quote: C2C12 cell lysate was generated using 5 million cells in RIPA lysis buffer with protease/phosphatase inhibitors (Roche #11836145001 and #04906837001), and 20μg of protein was loaded on a denaturing SDS page gel for detection of the VGLL2-NCOA2 fusion ...
-
bioRxiv - Developmental Biology 2020Quote: ... RT-qPCR reactions were conducted in a 10-µL final volume with 5 µL of 2X FastStar Universal SyBR green Master (Roche, Switzerland), 1.5 µL of forward/reverse primer mix (Table S1 ...
-
bioRxiv - Neuroscience 2021Quote: ... After that pelleted beads were gently resuspended in washing buffer containing 4xTBS, 5% NP-40, phosphatase inhibitors (10mM NaF, 1mM Na3VO4) and protease inhibitors (cOmplete®, Roche) and washed 10 times with gentle agitation ...
-
bioRxiv - Cancer Biology 2021Quote: ... 5% CO2 by quantification of LDH released into cell supernatants by apoptotic/necrotic cells (LDH detection kit, Roche Applied Science, #11644793001). Maximal lysis of the target cells (= 100% ...
-
bioRxiv - Neuroscience 2020Quote: ... the cDNA of our samples were diluted 10 fold and 4 µl were added to a mix of 5 µl FastStart Universal SYBR Green Master (Roche, Germany), 0.4 µl of nuclease-free water and 0.3 µl of forward and reverse primer (see primer sequences in Supplementary Table 1) ...
-
bioRxiv - Biochemistry 2020Quote: ... before lysis in swelling buffer (5 mM PIPES pH8.0, 85mM KCl) freshly supplemented with 1x protease inhibitor cocktail (Roche, cat. 04693116001) and 0.5% NP-40 ...
-
bioRxiv - Developmental Biology 2021Quote: ... The tissue was then washed with PBST extensively (10 times or more for 5 minutes) before development with BM-purple (1ml peer well, Roche Diagnostics). Time of development was approximately 24 hours at 4°C ...
-
bioRxiv - Microbiology 2022Quote: ... 50 µl of the post-nuclear supernatant was saved as the input lysate and 450 µl was incubated with 5 µg of anti-GFP antibody (Roche 11814460001) for 1 hour at 4°C ...
-
bioRxiv - Immunology 2022Quote: ... We transferred the minced tissue to a tube containing 5 mL of digestion Buffer containing collagenase D (2mg/mL, Roche #11088858001) and DNase (0.125 mg/mL ...
-
bioRxiv - Molecular Biology 2020Quote: ... for 40 cycles at 95°C for 5 s and 60°C for 30 s on a Light Cycler 480 Real-Time PCR System (Roche, Switzerland). All primer sequences used in this study are listed in Supplementary materials (Supplementary Table S1).
-
bioRxiv - Neuroscience 2020Quote: ... nt 1280-1350 from sequence NM_008553 detected with the mASCL1 probe (Universal Probe Library probe #74, Roche, Cat.No. 04688970001; 5′-(Fam)-GGCAGCAG-(Dark Quencher)-3′) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Telomeric repeats were probed by incubating the membrane with telomeric probe containing digoxigenin (5’CCCTAACCCTAACCCTAACCCTAA-DIG; Integrated DNA Technologies, Coralville, IA; Table S1) overnight at 42°C DIG Easy Hyb™ buffer (Roche). Blots were washed in 2X saline-sodium citrate (SSC ...