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Citations for Roche :
7501 - 7550 of 8781 citations for 6H Imidazo 4 5 e 2 1 3 benzothiadiazole 7 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2019Quote: ... 0.3 μL of 1 U/μL Kapa HiFi HotStart DNA polymerase (Kapa Biosystems, Inc., A Roche Company, Canada) and 0.75 μL of both forward and reverse 10 μM oligos ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 0.3 μL of 1 U/μL Kapa HiFi HotStart DNA polymerase (Kapa Biosystems, Inc., A Roche Company, Canada) and 0.75 μL of both forward and reverse 10 μM oligos ...
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bioRxiv - Molecular Biology 2019Quote: Crosslinked nuclei were extracted in 0.8ml modified RIPA lysis buffer (50mM Tris, 150mM NaCl, 0.5% sodium deoxycholate, 0.2% SDS, 1% NP-40, supplemented with: 1x antiprotease (Roche), RNasin (Promega) ...
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bioRxiv - Molecular Biology 2019Quote: ... The cDNA was diluted 1:10 before usage and amplified on a LightCycler 480 (Roche Diagnostics, Basel, Switzerland) using the Applied Biosystems SYBR Green Master Mix (Roche ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Whole cell extracts were prepared in HEPES whole cell lysis buffer (20 mM HEPES, pH 7.4, 1% TX-100,1x phosphoSTOP complete inhibition cocktail tablets [Roche] ...
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bioRxiv - Cancer Biology 2019Quote: ... washed and incubated with G6B antibody diluted 1:100 in Ventana’s DISCOVERY antibody diluent (Roche Cat#760-108) for 60 minutes at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: ... After a series of washes the probes were detected using FITC conjugated anti-Digoxigenin antibody (1:20, Roche) amplified with anti-sheep Alexa Fluor 488 (1:100 ...
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bioRxiv - Genetics 2019Quote: ... 150 µl of zirconia beads and 150 µl of lysis buffer (50 mM Tris [pH 7.5], 1 mM EDTA, 2.75 mM DTT, 1x PhosSTOP (Roche), 0.48 µg/ml Pefabloc SC (Sigma) ...
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bioRxiv - Molecular Biology 2019Quote: ... Nuclei were resuspended in wash buffer [(10 mM Tris-HCl pH 7.5, 100 mM NaCl, 1 mM EDTA pH 8.0, protease inhibitor (Roche)] and rotated for 10 min at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... for Quantitative RT-PCR (Supplemental Table 1) and amplified in a Roche Light Cycler 480 (Roche, Basel, Switzerland). Relative expression was quantified using Ct values ...
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bioRxiv - Microbiology 2019Quote: ... The total protein pellets were resuspended in 1 mL NP-40 with protease inhibitor cocktail (Roche Diagnostics GmbH). Samples were pre-cleared using Protein A/G plus agarose beads (Pierce ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated for 1 h with rat anti-HA high affinity monoclonal antibodies (Roche Applied Science, Penzberg, Germany) at 1:500 ...
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bioRxiv - Biochemistry 2019Quote: ... 1 mM EDTA and 0.5% NP-40 supplemented with Complete Mini EDTA-free Protease inhibitor cocktail tablets (Roche)) and left to incubate for 20 minutes on ice ...
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bioRxiv - Genomics 2019Quote: ... 0.5 mM EGTA,1% Triton X-100, 0.1% SDS, 0.1% Na-deoxycholate, 140 mM NaCl, 1x protease inhibitors-Roche). 150 ul of sonicated chromatin was then incubated 4h at 4°C on a rotating wheel with 3 µg of antibody conjugated to 10 µl of Dynabeads ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in 1ml of cold Lysis Buffer 1 (10mM Tris pH 8.0, 10mM NaCl, 0.2% IGE-PAL, Protease inhibitor (Roche)) and incubated on ice for 15 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... High Salt buffer (50 mM HEPES pH 7.5, 500 mM NaCl, 1% Triton X-100, 1x protease (Roche) and phosphatase inhibitors (Sigma-Aldrich) ...
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bioRxiv - Physiology 2020Quote: ... and 1 mM phenylmethylsulfonyl fluoride (PMSF)) supplemented with EDTA-free protease inhibitor cocktail and phosphatase inhibitors cocktail (Roche). Tissue lysates were centrifuged at 14,000 rpm for 20 min at 4°C twice before using the supernatant for western blot studies ...
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bioRxiv - Developmental Biology 2019Quote: ... All three vectors were transfected into DLD-1 TIR1 cells using Xtreme Gene 9 DNA transfection reagent (Roche). Cells were sorted based on their YFP fluorescence and single clones were isolated ...
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bioRxiv - Cell Biology 2019Quote: Control BMSCs were lysed by incubation with RIPA buffer (Pierce) and 1 mM protease inhibitors (Complete Mini, Roche). Samples were incubated with 5X Lane Marker Reducing sample buffer (Pierce ...
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bioRxiv - Microbiology 2021Quote: ... Plasma viral load in ART-treated PLWH was measured using the Amplicor HIV-1 monitor ultrasensitive method (Roche).
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bioRxiv - Molecular Biology 2020Quote: ... 2.5 mM MgCl2) supplemented with 0.5% Triton X-100 and 1 pill Complete® protease inhibitor cocktail (Roche). The cell lysates were subjected to two 5 sec pulses of sonication at the lowest settings (Branson Sonifier 250 ...
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bioRxiv - Microbiology 2021Quote: ... and polymerized by UV light prior to labeling with anti-HA antibody (Roche, clone 3F10, 1:40 dilution) and 10nm gold bead conjugated goat anti-rat (Electron Microscopy Sciences ...
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bioRxiv - Genetics 2020Quote: ... then resuspended in 600 µl of ChIP lysis buffer (50mM HEPES, pH 7.5, 140mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, one tablet Roche cOmplete mini EDTA-free Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells are pelleted and resuspended in 1 mL of Lysis Buffer (200 mM NaCl, 25 mM HEPES, 1 mM MgCl2, 0.2 mM EDTA, 0.5% NP-40, 1X Roche complete inhibitor ...
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bioRxiv - Microbiology 2019Quote: ... Cells were washed with PBS twice and lysed with lysis Buffer II (10 mM Tris-HCl pH 7.5, 100 mM NaCl, 0.5% NP-40, 1 mM EDTA and EDTA-free protease inhibitors cocktail [Roche]). As a control of protein synthesis inhibition ...
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bioRxiv - Molecular Biology 2021Quote: Lysates for Western-blot were made in a 1% triton buffer including cOmplete protease inhibitor cocktail (Roche 11697498001) and Western-Blot and flow cytometric staining was performed as described previously (41),(42) ...
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bioRxiv - Microbiology 2020Quote: ... 0.05 μL of 20 μg/μL (1 μg BSA/reaction) of nonacetylated bovine serum albumin (BSA, 10711454001, Roche), 1 μL SuperScript™ III RT/Platinum Taq Mix and 1.85 μL of primer/probe mix (2019-nCoV CDC EUA Kit ...
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bioRxiv - Microbiology 2021Quote: ... A 1 uL aliquot was used to amplify the V1-V3 region using HiFi HotStart ReadyMix (KAPA BioSystems) and the Illumina PCR protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Detection of hybridized probes was performed with anti-DIG antibodies AP fragments (Roche, Basel Switzerland; dilution 1:1000) overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... and lysed by resuspension in lysis buffer (150 mM NaCl, 50 mM Tris-HCl pH 7.4, 1 mM EDTA, 0.5% NP-40, and protease inhibitor [Roche]) with rotation at 4°C for 30 min ...
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bioRxiv - Immunology 2019Quote: ... Cells were washed twice with PBS before lysis in 0.1ml lysis buffer (1% Triton X-100, 0.1% SDS, PBS, Complete EDTA-free protease inhibitor [Roche]). Total protein concentration of lysates was quantitated using the Pierce BCA assay (cat no 23225 ...
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bioRxiv - Immunology 2019Quote: ... and cells were lysed in plate in RIPA buffer (50 mM Tris, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 1mM PMSF, 1x Roche protease inhibitor tablet [no EDTA] ...
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bioRxiv - Cell Biology 2019Quote: ... Fat pads were minced and digested in a solution of collagenase type D (Roche, 11088866001, 1 mg/ml) and Dispase II (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... 10mM Imidazole) supplemented with 1mg.mL−1 of lysozyme and a mix of EDTA-free protease inhibitor (cOmpleteTM, Roche) and lysed by sonication (4x[5*10s] ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were harvested and the pellet was suspended in ice cold lysis buffer (50mM Tris HCl [pH 7.5], 400mM NaCl, 0.1mM PMSF, 1mM DTT, and 1 protease inhibitor tablet (Roche)) ...
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bioRxiv - Genomics 2019Quote: ... 0.5 mM EGTA,1% Triton X-100, 0.1% SDS, 0.1% Na-deoxycholate, 140 mM NaCl, 1x protease inhibitors-Roche) and once with Buffer-C (10 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell proliferation was assessed by WST-1 assay as per manufacturer’s protocol (Roche Applied Science, Indianapolis, IN, USA). Colony formation assay was performed with MethoCult methylcellulose-based media as per manufacturer’s protocol (Stemcell Technologies ...
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bioRxiv - Genetics 2020Quote: ... 200 μl lysis buffer (8M Urea, 1% SDS, 50 mM Tris pH 8.5, Roche protease and phosphatase inhibitor) were added to the pellet and samples were vortexed and sonicated on ice ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM benzamidine) supplemented with 0.1 mg/mL lysozyme and one Complete EDTA-free protease inhibitor tablet (Roche) per 100 mL ...
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bioRxiv - Genomics 2021Quote: ... 250 nL of protease mix was added (1× NEB CutSmart buffer, 1.0 mg/mL Proteinase K (Roche, 000000003115836001)) ...
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bioRxiv - Biochemistry 2020Quote: ... 250 mM imidazole) and incubated after addition of 1 or 10 mM phenylmethanesulfonyl fluoride (PMSF) (Roche, Basel, Switzerland), or 1 mM H2O2 (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: Cell proliferation in LNCaP IL1B overexpression and vector cells was assessed using Cell Proliferation Reagent WST-1 (Roche), per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 10 ml of DMEM media and 1 mg/ml collagenase D (Sigma-Aldrich, COLLD-RO Roche, #11088866001). The tubes were placed on a gentleMACS Dissociator (MACS Miltenyi Biotec ...
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bioRxiv - Microbiology 2020Quote: ... qPCR was carried out using LightCycler® 480 SYBR Green 1 Master reaction mix (Roche Diagnostics, Mannheim, Germany) following the manufacturer’s temperature program with 62°C as the primer hybridization and elongation temperature ...
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bioRxiv - Microbiology 2019Quote: ... pH 7.4, 150 mM NaCl, 0.5 mM EDTA, 1% digitonin [Sigma], supplemented with 1X complete protease inhibitor [Roche]). After lysis on ice for 10 min ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were lysed in lysis buffer (1.5M NaCl, 20 mM Phosphate buffer pH 7.5, 20 mM Imidazole, 10mg/mL lysozyme, 1 tablet of Roche EDTA-free protease inhibitor cocktail Millipore Sigma 11873580001 ...
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bioRxiv - Immunology 2021Quote: ... minced thymuses from fetuses and postnatal mice were digested with 0.5 and 1 unit/ml Liberase TM (Roche) in the presence of 0.01% DNase I (Roche) ...
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bioRxiv - Developmental Biology 2021Quote: ... wild-type or transgenic animals in an equal volume of Pedro’s buffer (30mM HEPES, pH 7.5, 100mM KOac, 10mM EDTA, 10% glycerol, 1 mM DTT, 1x Roche complete mini protease inhibitors ...
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bioRxiv - Immunology 2021Quote: ... and digested for 20 min at 37 °C in medium supplemented with 1 mg/mL Collagenase D (Roche) and 2000 U/mL DNase I (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... In situ hybridization signals were detected with sheep anti-digoxigenin-AP Fab fragments (1:10000; Roche Diagnostics, Germany) conjugated with alkaline phosphatase ...