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Citations for Roche :
701 - 750 of 7633 citations for ssc mir 758 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... with SYBR Green RT-PCR (Roche, #4913914001). Melting curve analysis was performed for each primer set to ensure the specificity of the amplified product and with an efficiency of 2 ...
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bioRxiv - Physiology 2022Quote: ... RT-PCR assays using the Lightcycler (Roche) with Roche universal probes and primer pairs are specified (Supplementary Table S1) ...
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bioRxiv - Genetics 2020Quote: ... Real‐time qPCR was performed on LightCycler instrument (Roche) with GAPDH as internal control.
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bioRxiv - Immunology 2023Quote: LightCycler 480 II Real-Time System (Roche, Basel, Switzerland), or Applied Biosystems 7500 Fast Real-Time PCR System (ThermoFisherScientific ...
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bioRxiv - Cell Biology 2024Quote: ... Real-time qPCR with SYBR Green Master Mix (Roche) was performed to evaluate AAV9 titers.
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bioRxiv - Plant Biology 2020Quote: ... Relative quantification analysis was performed using the LightCycler® 480 Real-Time PCR System software (v1.5.0 SP3, Roche Applied Science). Efficiency values for each amplicon were calculated by LinReg PCR software (Ramakers et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... for genes of interest and Gapdh (a normalization control for amplification efficiency) on a LightCycler 480 Real-Time PCR machine (Roche). The primer sequence used for qPCR analysis is listed in Supplementary Table 12 ...
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bioRxiv - Developmental Biology 2021Quote: ... We used a 7900HT Fast Real-Time PCR System with 384-Well Block Module (Applied Biosystem) with Faststart universal SYBR Green Master (rox) (FSUSGMMRO Roche). The relative fold change for each gene expression was calculated by comparing treated and untreated conditions ...
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bioRxiv - Microbiology 2021Quote: ... Real-time PCR was performed with the specific primers listed in Table 2 using the LightCycler 480 (Roche, Manheim, Germany).
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bioRxiv - Molecular Biology 2021Quote: ... Circular RNA levels were quantified by quantitative Real-Time Polymerase Chain Reaction (qRT-PCR), using Sybr Green mix (dssTakara, Japan) and detected by Lightcycler LC 480 (Roche). Primer sequences for qRT-PCR have been summarised in Supplementary Table 2.
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bioRxiv - Developmental Biology 2022Quote: ... and the complementary DNA (cDNA) obtained was used for real time quantitative PCR using LightCycler instrument (Roche Life Science, Germany). cDNA was quantified using TaqMan Gene expression assays and the TaqMan primers to target genes were purchased from Thermo Fisher Scientific listed in Table 1 ...
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bioRxiv - Cancer Biology 2021Quote: ... The candidate cells were selected with 2 µg/ml puromycin for a week and the selected cells were analysed by High-resolution melting peaks analysis using real-time PCR (Light cycler 480 II, Roche).
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bioRxiv - Immunology 2021Quote: ... Relative gene expression of RORγt and FOXP3 was performed using Real Time PCR (Lightcycler 480, Roche Molecular System, Pleasanton, CA), SYBR Green and primers specific to RORγt ...
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bioRxiv - Plant Biology 2021Quote: ... The abundance of specific transcript was measured by probing 1 μL cDNA by quantitative real-time PCR in a total volume of 10 µl containg 5 μL SYBR-green master mix (Roche), 0.5 μM forward and reverse primer and water ...
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bioRxiv - Cell Biology 2020Quote: ... The semiquantitative real-time PCR was proceeded with the faststart universal SYBR green master from Roche (Ref. 04913914001, lot. 11929100) using gene-specific primers ...
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Telomerase deficiency in humans is associated with systemic age-related changes in energy metabolismbioRxiv - Cell Biology 2022Quote: Whole blood telomere lengths were measured using the monochrome multiplex quantitative PCR method (modified from (Cawthon, 2009) on a LightCycler 480 real time thermocycler (Roche). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... the reaction was performed with 2 x Hieff qPCR SYBR Green Master Mix (Yeasen) and detected by LightCycle 480 Real-Time PCR machine (Roche). For RNA-seq ...
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bioRxiv - Cancer Biology 2022Quote: ... The obtained cDNA was used as template in a real-time quantitative PCR reaction using the SYBR Green master mix (Roche) and a LightCycler 1.5 (Roche ...
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bioRxiv - Molecular Biology 2020Quote: ... were carried out with the Applied Biosystems 7500 real-time PCR system with FastStart universal SYBR green master mix (Roche). All values were normalized to 18S RNA levels.
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bioRxiv - Immunology 2022Quote: ... pursued by 95°C for 3 min and then 45 cycles at 95°C for 15 sec and 58°C for 30 seconds using a LightCycler-480 Real-Time PCR system (Roche). The primers and the probes were designed against the E gene17.
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bioRxiv - Plant Biology 2019Quote: ... Quantitative RT-PCR analysis was carried out using the ABI 7300 Real Time PCR System with Fast Start Universal SYBR Green Master Mix and ROX (Roche), and data were analyzed using the 2-ΔΔCT method ...
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bioRxiv - Cell Biology 2019Quote: ... Real-time PCR was performed with Thunderbird SYBR qPCR Mix (Toyobo, QPS-201) or KOD SYBR qPCR Mix (Toyobo, QKD-201) on a LightCycler 480 Real-Time PCR System (Roche). The relative expression level of each transcript was normalized by Gapdh expression levels ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The cDNA templates were then used for Sybr green real-time PCR to determine the transcription level of viral genes with a Light Cycler 480 system (Roche).
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bioRxiv - Cell Biology 2020Quote: ... The concentration of viral genomes (vg/ml) was determined by quantitative real-time PCR on a LightCycler480 (Roche diagnostic, France) by using TaqMan probe ...
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bioRxiv - Genomics 2021Quote: ... was performed with NZY Speedy qPCR Green Master mix (NZY tech) and in a LightCycler 480 Real-Time PCR System (Roche). Primer sequences are detailed in the Supplementary Table S8 ...
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bioRxiv - Immunology 2020Quote: ... The abundance of transcripts from the genes of interest was measured by quantitative real-time PCR with the Light Cycler 480 II system (Roche) with a Brilliant III SYBR master mix (Agilent ...
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bioRxiv - Molecular Biology 2020Quote: ... Real-Time qRT-PCR was performed using the Real-Time 2xHS-PCR Master Mix Sybr B (A&A Biotechnology) on a LightCycler 480 II qPCR System (Roche) with custom-designed primers for Gli1 (Fwd ...
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bioRxiv - Microbiology 2022Quote: ... the V4 region of the 16S rRNA gene was amplified with barcode primers containing the index sequences using a KAPA HiFi HotStart Real-time PCR Master Mix (Roche). We monitored PCR product amplification and concentration on a Bio-Rad CFT Connect Real-Time PCR system ...
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bioRxiv - Molecular Biology 2023Quote: ... Real-time reverse transcription PCR assays were conducted in 96-well plates using the LightCycler 480 Instrument (Roche, Wilmington, MA). Levels of mRNA expression were normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) ...
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bioRxiv - Plant Biology 2022Quote: ... The abundance of specific transcript was measured by probing 1 μL cDNA by quantitative real-time PCR in a total volume of 10 μl containg 5 μL SYBR-green master mix (Roche), 0.5 μM forward and reverse primer and water ...
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bioRxiv - Immunology 2022Quote: RU5 and Gag reverse transcripts and integrated HIV-DNA levels were quantified by nested real-time PCR (Light Cycler 480, Roche). Experiments were performed in triplicates on 0.5-1×105 T-cells/PCR ...
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bioRxiv - Genomics 2023Quote: ... supplemented with 0.2μM of specific primer pairs (Table S9) and run on a light cycler rapid thermal system (LightCycler®480 2.0 Real time PCR system, Roche) with 20 sec of denaturation at 95°C ...
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bioRxiv - Biochemistry 2022Quote: ... A quantitative real-time-PCR analysis was performed with a Roche Light Cycler 480 System (Roche Applied Science, Penzberg, Germany) using SYBR Premix (Takara ...
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bioRxiv - Developmental Biology 2023Quote: ... The real-time PCR was performed with Roche LightCycler 480 SYBR GREEN I Master mix on a LightCycler 480 system (Roche). Relative gene expression abundance between samples was calculated with the CT method using Gapdh as an internal control ...
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bioRxiv - Immunology 2023Quote: ... and gene expression assessed using a BioMark HD (Fluidigm) according to the manufacturer’s instructions or a LightCycler® 480 Real-Time PCR System (Roche). The annealing temperature was 62°C ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was isolated by phenol-chloroform extraction and ethanol precipitation and analyzed by qPCR using Light Cycler Real-Time PCR System (Roche) and iTaq Universal SYBR Green Supermix SYBR Green Master Mix (BioRad ...
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bioRxiv - Cancer Biology 2023Quote: ... quantitative real-time PCR was performed in duplicates in 384-well plates using Fast Start SYBR Green Master Rox (Roche) on a QuantStudio™ 5 real-time PCR system (Applied Biosystems ...
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bioRxiv - Cancer Biology 2023Quote: ... Reverse transcription was performed using qScript (Quanta Biosciences) followed by real-time PCR using KAPA SYBR FAST ROX Low (Roche) on the ViiA 7 Real-Time PCR instrument (Applied Biosystems) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Quantitative real time PCR was performed using the Luna Universal qPCR Mastermix (NEBiolabs) and the Light Cycler 480 system (Roche). Actin and eIF4A homologs were used as reference genes as established by Arai and collaborators (Arai et al ...
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bioRxiv - Cell Biology 2024Quote: ... The quantitative real-time PCR was done using Maxima SYBR Green/ROX qPCR Master Mix (2X) (Fermentas) and LightCycler 480 (Roche) quantitative PCR machine ...
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bioRxiv - Pathology 2024Quote: ... cDNA product was 1 to 40 diluted and real-time PCR was performed using FastStart SYBR Green Master Mix (Roche) on QuantStudio 5 (ThermoFisher ...
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bioRxiv - Cell Biology 2024Quote: ... The quantitative real-time PCR was done using Maxima SYBR Green/ROX qPCR Master Mix (2X) (Fermentas) and LightCycler 480 (Roche) quantitative PCR machine ...
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bioRxiv - Microbiology 2020Quote: ... quantitative RT-PCR was performed using KAPA SYBR FAST qPCR Master Mix Kit (Kapa Biosystems) on a LightCycler 480 Instrument II (Roche ...
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bioRxiv - Microbiology 2023Quote: ... RT-PCR reactions were set up using the FastStart Essential DNA Green Master kit (Roche), using four different primer pairs ...
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bioRxiv - Pathology 2020Quote: ... these protocols were adjusted to a real-time format with Green Essential FastStart Master kit (Roche Diagnostics), in a Nano LightCycler (Roche Diagnostics) ...
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bioRxiv - Cell Biology 2022Quote: ... Two different HRMA-compatible platforms were used (Applied Biosystems ViiA 7 Real-Time PCR System, using the MeltDoctor HRM Master Mix, and Roche LightCycler 480 System ...
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bioRxiv - Physiology 2019Quote: ... The reverse transcription product was diluted according to the efficiency curve and submitted in duplicates to real-time quantitative PCR using LightCycler® 480 SYBR Green I Master (Roche) in 7500 System (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... mgc2 adhesin and mgc2-like adhesin (list of primers used in Table 2) were made by real-time PCR (rtPCR) on LightCycler 96 thermocycler (Roche®). The LightCycler SYBR Green I Master reaction mix (Roche® ...
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bioRxiv - Neuroscience 2020Quote: ... Results were obtained with the Basic Relative Quantification Analysis of the LightCycler® 480 Real-Time PCR System software (Roche, Switzerland) and analyzed using the ΔΔCt method ...
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bioRxiv - Biochemistry 2021Quote: ... The expression of targeted genes and GAPDH transcripts were monitored by LightCycler®480 Real-time PCR (Roche Diagnostics GmbH, Germany) using gene-specific primers (Eurofins ...