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Citations for Roche :
701 - 750 of 7066 citations for Urea Nitrogen BUN Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... and co-transfected into 60-70% confluent BHK cells grown in 6-well plate tissue culture plates using Fugene HD transfection reagent (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Cells were transfected with 250-800 ng DNA per well of a 12-well plate or 500 ng per well of a 6-well plate using X-tremeGENE 9 DNA transfection reagent (Roche), and harvested after 24 h.
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bioRxiv - Microbiology 2020Quote: ... thus one negative control is placed after every 5 samples on a Light cycler 480 multiwell plates 96-well plate (Roche).
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bioRxiv - Neuroscience 2020Quote: ... All qPCR reactions were performed in 10 μl volume in triplicates with 1× LightCycler 480 SYBR Green I Master kit (Roche) and primers listed in Supplementary Table 1 on LightCycler® 480 PCR instrument II (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNAse was inactivated with 1 µl of Stop Solution at 65°C for 10 min and RT was performed using Transcriptor First Strand cDNA Synthesis Kit (Ref. 04 897 030 001, Roche) in a final volume of 20 µl ...
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bioRxiv - Neuroscience 2021Quote: ... 1μl of cDNA diluted with 10-fold MQ was mixed with KAPA SYBR Fast qPCR kit and amplified with Lightcycler 96 (Roche; 95℃ 5 min ...
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bioRxiv - Cancer Biology 2022Quote: ... 2-10 5 micron sections were extracted using the High Pure FFPE RNA Isolation kit (Roche Life Sciences, Penzberg, Germany) under RNase free conditions following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and an additional step 72°C for 10 min performed on a Light Cycler 480 with the SYBR Green I Master kit (Roche) (qPHD UM2/GenomiX Platform ...
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bioRxiv - Cell Biology 2019Quote: ... and the RealTime ready Custom Panels plates (Roche) used for the assay ...
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bioRxiv - Systems Biology 2019Quote: ... in 96-well plates using SYBR Green (Roche) for selected transcript sequences with two technical replicates for each of the three independent biological replicates ...
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bioRxiv - Cancer Biology 2021Quote: ... in a Lightcycler 480 384-well plate (Roche), and analyzed using Lightcycler 480 Software v1.5 (Roche) ...
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bioRxiv - Neuroscience 2023Quote: ... onto a LightCycler multiwell 384 white plate (Roche). 3uL of the qPCR detection master mix (Table S4 ...
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bioRxiv - Genomics 2024Quote: ... Cell pellets were resuspended in 250 µL ice-cold Hi-C lysis buffer (10 mM Tris-HCl pH 8.0, 10 mM NaCl, 0.2% Igepal CA630, 1 tablet/10 mL Roche complete mini EDTA-free protease inhibitor) ...
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bioRxiv - Microbiology 2021Quote: ... 0.2 pmoles of gene-specific forward primer and 0.2 pmoles of gene-specific reverse primer was combined with 2 µL of cDNA template into each well of a 96-well plate (LightCycler® 480 Multiwell Plate 96, white, 04729692001, Roche). qPCR amplification was performed using the following cycling conditions ...
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bioRxiv - Genetics 2023Quote: Cell migration assays were performed with the xCELLigence Biosensor System using specifically designed 16-well plates equipped with membranes having 8-μm pores (CIM-plate 16; Roche Diagnostics). Cells in serum-free medium were seeded in the upper chambers ...
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bioRxiv - Neuroscience 2019Quote: ... and incubated in HNPP/Fast red working solution according to manufacturer’s instructions (HNPP Fluorescent Detection Set, Roche). To stop the reaction ...
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bioRxiv - Genetics 2019Quote: Detection of tRNA precursors was performed as described (Otsubo et al., 2018) using Digoxigenin labeled probes (Roche), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies were detected using an anti-rabbit HQ detection system (catalog # 7017936001 and 7017812001, Roche Diagnostics) followed by Chromomap DAB IHC detection kit (catalog # 5266645001 ...
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bioRxiv - Physiology 2022Quote: ... Detection of DIG-labeled probes was performed by incubating with anti-DIG antibody (1:2000, Roche #11093274910) in 0.1% PBST overnight at 4° C ...
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bioRxiv - Microbiology 2021Quote: ... Southern blot analyses were performed as previously described using the digoxigenin-anti-digoxigenin detection system (Roche Diagnostics)38.
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bioRxiv - Microbiology 2022Quote: ... and the reactions were run in a Light Cycler 480 II sequence detection system (Roche, Shanghai, China). The rpoC and gyrA genes were used as reference ...
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bioRxiv - Cancer Biology 2023Quote: Cell proliferation and cell death were assessed using the WST-1 and cytotoxicity Detection KitPLUS (LDH) (Roche) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... bound antibodies were detected with an anti-mouse HQ detection system (catalog# 7017936001 with 7017782001, Roche Diagnostics) and a Chromomap DAB IHC detection kit (catalog # 5266645001 ...
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bioRxiv - Molecular Biology 2023Quote: ... Detection with anti-digoxigenin antibody conjugated with alkaline phosphatase (Anti-Digoxigenin-AP Fab fragments, 11093274910, Roche, Switzerland) and activation with the chemiluminescent substrate CDP-Star (11759051001 ...
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bioRxiv - Genomics 2019Quote: ... was the substrate for a 10 μL qPCR reaction using either Taq DNA polymerase or KAPA HiFi (KAPA HiFi HotStart PCR kit, Kapa Biosystems) according to the manufacturer’s protocol with barcoded P5 and P7 sequencing primers at 0.5 mM and with 1x EvaGreen dye (Biotium ...
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bioRxiv - Genomics 2019Quote: ... Quality control for primer target amplification and performance in multiplex PCR was assessed by screening each primer pair in singleplex reactions using gDNA from PC3 or DU145 cells (10 ng) as template (see below) using the HiFi HotStart Uracil+ ReadyMix PCR Kit (Kapa Biosystems). Reactions were supplemented with uracil N-glycosylase (0.2 U ...
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bioRxiv - Genomics 2019Quote: All libraries were amplified off the array using the primers indicated in Supplemental Table 10 with KAPA HiFi HotStart Uracil+ ReadyMix PCR Kit (Kapa Biosystems) as described above ...
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bioRxiv - Genomics 2021Quote: ... Strand-specific Illumina cDNA libraries were prepared using the KAPA Stranded RNA-Seq library preparation kit with 10 cycles of PCR (KAPA Biosystems). Library quality was assessed by BioAnalyzer (Agilent ...
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bioRxiv - Genetics 2020Quote: ... Amplicons were pooled to 10 nM in EB buffer and the final concentration was determined using the Illumina Universal qPCR Amplification kit from Kapa Biosystems. All pooled samples were diluted to 4 nM ...
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bioRxiv - Neuroscience 2021Quote: ... 10 ng of cDNA were used for qPCR with the KAPA SYBR FAST qPCR Kit (KK4604, KAPA Biosystems, Wilmington, MA, USA) and the experiment run on the Biorad CFX96 system using the TqPCR protocol described in Zhang et al ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The libraries were diluted 1:10 000 and 1:20 000 and quantified in triplicate using the KAPA Library Quantification Kit (Kapa Biosystems). Equimolar samples were pooled ...
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bioRxiv - Immunology 2023Quote: ... DNA was incubated for 10 minutes at 65°C before performing RT qPCR using the KAPA SYBR FAST qPCR Kit from KAPA Biosystems. Primers used for real-time qPCR can be found in Supplementary Table 1.
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bioRxiv - Genomics 2024Quote: ... We used 10 ng of DNA from two biological replicates to generate sequencing libraries using KAPA Hyper Prep Kit (KAPA Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative real time PCRs (qPCRs) were performed in a total volume of 10 µl with Kapa SYBR Fast qPCR kit (KAPA Biosystems) on an ABI 7500 fast machine operated with ABI 7500 software (Applied Biosystems) ...
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bioRxiv - Cell Biology 2020Quote: ... HUVEC were pre-treated with siRNAs or drug for 24 hr prior to plating an equal cell number onto the microelectrode surface of the E-plate (E-plate 16, Roche Applied Science). Impedance readings were taken automatically every 5 min for 24 hr.
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bioRxiv - Biophysics 2019Quote: ... and loaded in LightCycler 480 Multiwell Plate 96 (Roche) for DSF assay ...
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bioRxiv - Physiology 2022Quote: ... in a LightCycler LC480 plate reader (Roche Life Science). For each cDNA sample ...
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bioRxiv - Microbiology 2021Quote: ... on 384-well plates using a LightCycler 480 (Roche) following manufacturer recommendations and the primers shown in Table S2 ...
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bioRxiv - Genomics 2021Quote: ... in 384-well plates on a LightCycler480 device (Roche) as described in (Varrault et al ...
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bioRxiv - Genetics 2022Quote: ... and LightCycler® 480 Multiwell Plate 384 (Roche, # 04729749001) were used according to the manufacturers’ recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... in 384-well plates on a LightCycler 480 (Roche) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Reactions were performed in LightCycler 480 Multiwell Plates (Roche) in a LightCycler 480 (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... with 10 U PNK in 10 µl with 20 U RNasin (Roche). After 1 h ...
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bioRxiv - Cell Biology 2019Quote: ... 10 mM Hepes pH 7.9) containing 10 % proteinase inhibitor cocktail (PIC, Roche) and lysed in RIPA buffer + 1x PIC ...
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bioRxiv - Cell Biology 2020Quote: ... Japan) was employed for detection of proteins visualized by Lumi-light Plus Western blotting substrate (Roche, Basel, Switzerland).
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bioRxiv - Cell Biology 2020Quote: ... followed by incubation with HRP-conjugated rabbit anti-sheep antibodies and detection using ECL reagents (Roche Diagnostic GmbH). A series of timed exposures were undertaken to ensure that densitometric analyses were performed at exposures within the linear range ...
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bioRxiv - Immunology 2021Quote: ... The SYBR Green Dye detection system was used for quantitative real-time PCR on Light Cycler 480 (Roche). Controls consisting of ddH2O were negative for target and housekeeping genes ...
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bioRxiv - Physiology 2022Quote: Real time quantitative PCR amplification reaction were carried out in a LightCycler 480 detection system (Roche, Basel, Switzerland) using the LightCycler FastStart DNA Master plus SYBR Green I kit (catalog n° 03515869001 ...
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bioRxiv - Cell Biology 2019Quote: ... Japan) was employed for detection of proteins visualized by Lumi-light Plus Western blotting substrate (Roche, Basel, Switzerland).
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bioRxiv - Neuroscience 2022Quote: ... RT-PCR was carried out using SYBR Green master mix for detection in Light cycler LC 480 (Roche). All primers used for qRT-PCR are given in Supplementary Table S1.The endogenous control GAPDH was used to normalize quantification of the mRNA target.