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Citations for Roche :
701 - 750 of 1541 citations for 4 Nitrophenyl beta D glucuronic acid sodium salt since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Cells were lysed with 4% SDS lysis buffer (4% SDS, 150 mM NaCl, 50 mM triethanolamine pH 7.4, Roche protease inhibitor, benzonase). Protein concentrations were determined by the BCA assay (Pierce) ...
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bioRxiv - Physiology 2024Quote: ... fixed with 4% PFA for 15 min and stained with 4′,6-diamidino-2-phenylindole (DAPI) (0.1 µg/mL; Roche, cat no. 10236276001) for 10 min at RT ...
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bioRxiv - Genomics 2024Quote: ... for which we could confirm their differential methylation between 4 Col-WT and 4 Col-ddm1 plants by comparing digested and non-digested samples using qPCR (Roche LightCycler 480). We then measured methylation states using this method on DNA extracted using Macherey-Nagel 96-well plate extraction kit (Macherey-Nagel ...
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bioRxiv - Developmental Biology 2020Quote: ... 5-Bromo-4-chloro-3-indolyl phosphate (BCIP, [Roche]) and nitro blue tetrazolium chloride (NBT ...
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bioRxiv - Cancer Biology 2021Quote: ... at 4 °C overnight and protein G-Agarose (Roche) at 4 °C for 3 h ...
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bioRxiv - Biochemistry 2020Quote: ... 4 cOmplete EDTA-free Protease Inhibitor Cocktail tablets (Roche), 500 U benzonase ...
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bioRxiv - Neuroscience 2020Quote: ... levodopa (Madopar®, Roche, Levodopa/carbidopa, ratio 4:1) was administered twice daily for 4-5 months at an individually-tailored dose designed to produce a full reversal of the parkinsonian condition (p.o ...
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bioRxiv - Cancer Biology 2021Quote: ... 4 mL of Red Blood Cell Lysis Buffer (Roche) were added before being gently rocked for 10 min at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... 5-bromo-4-chloro-3-indolyl-phosphate (BCIP, Roche), and levamisole (1359302 ...
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bioRxiv - Microbiology 2024Quote: ... (4) we used KAPA HiFi master mix (Roche, 07958935001) and 19 cycles of PCR.
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bioRxiv - Cancer Biology 2024Quote: ... SDS 0.4%) + Proteinase-K 4 mg/ml (Roche, 3115887001) and incubated for 2h at 55°C ...
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bioRxiv - Developmental Biology 2023Quote: ... 5-Bromo-4-chloro- 3-indolyl phosphate (BCIP, [Roche]) and nitro blue tetrazolium chloride (NBT ...
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bioRxiv - Developmental Biology 2024Quote: ... 4 μL of 20x PhosStop® phosphatase inhibitors (Roche), 4 μL of 20x cOmplete® protease inhibitors (Roche ...
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bioRxiv - Developmental Biology 2024Quote: ... 4 μL of 20x cOmplete® protease inhibitors (Roche) and 1.6 μL of 1 M DTT ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM sodium molybdate) and protease inhibitors (1 mini-Complete EDTA-free tablet per 10 ml lysis buffer; Roche Life Sciences)) and sonicated three times for 15 sec each with intermittent cooling on ice ...
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bioRxiv - Genetics 2021Quote: Genomic DNA was extracted from the DsRed2-positive G1 adult animals by standard sodium dodecyl sulfate (SDS) lysis-phenol buffer after incubation with proteinase K (Roche, 3115879001), followed by RNase A (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... 1mM PMSF, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 1x Roche Complete Protease Inhibitor Cocktail, and 1x Roche Phosphatase Inhibitor Cocktail). Protein concentrations were measured using a BCA kit (Pierce) ...
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bioRxiv - Microbiology 2021Quote: ... 1 mM sodium molybdate) and protease inhibitors (1 mini-Complete EDTA-free tablet per 10 ml lysis buffer; Roche Life Sciences)) and sonicated three times for 15 sec each with intermittent cooling on ice ...
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bioRxiv - Immunology 2022Quote: ... counted and lysed in m-RIPA buffer (1% NP40, 1% sodium deoxycholate, 150mM NaCl, 50mM Tris pH 7.5, Complete protease inhibitors; Roche, Basel, Switzerland). CANX and T6BP were immunoprecipitated using anti-CANX antibody PA5-34754 (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: Cryo-lysed cells were resuspended in lysis buffer (40mM Tris pH 8.0, 200mM sodium citrate) with 1× complete Mini EDTA-free protease-inhibitor solution (Roche Life Science). After centrifugation at 14,000×g for 10 min at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... 1mM EDTA, 10 mM Sodium butyrate, 0.1% deoxycholate, 0.1% SDS, 1% Triton X-100, 1mM PMSF and 1 × Roche protease inhibitor Cocktail). The obtained chromatin was sonicated into DNA fragments between 200-500 bp using Diagenode Bioruptor (high setting ...
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bioRxiv - Molecular Biology 2022Quote: ... Pellets were thawed and lysed in 300 µl FA lysis buffer (50 mM HEPES–KOH pH 8.0, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 1 mM PMSF, 1x Roche protease inhibitor) with ∼1 ml ceramic beads on a Fastprep-24 (MP Biomedicals) ...
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Genome modularization reveals overlapped gene topology is necessary for efficient viral reproductionbioRxiv - Synthetic Biology 2020Quote: ... sample pellets were resuspended in 200 µL lysis buffer (100 mM triethylammonium bicarbonate buffer (TEAB) with 1% (v/v) sodium deoxycholate (SDC) and 1x protease inhibitor cocktail solution (Roche, Switzerland)) ...
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bioRxiv - Microbiology 2021Quote: ... The adherent cells were lysed with ASM lysis buffer (250 mM sodium acetate, pH 5.0, 0.1% Nonidet P-40, 1.3 mM EDTA, and 1x Roche Complete protease inhibitor cocktail) for at least 15 min and were scraped off with a pipette tip and collected ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM sodium molybdate) and protease inhibitors (1 mini-Complete EDTA-free tablet per 10 ml lysis buffer; Roche Life Sciences)) and sonicated three times for 15 seconds each with intermittent cooling on ice ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were size fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred into a polyvinylidene difluoride membrane (Roche, Basel, Switzerland). Membranes were incubated (overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... then resuspended in 880 uL RIPA buffer (1x PBS, 1% NP-40, 0.5% Sodium deoxycholate, 0.1% SGS, Roche protease inhibitor cocktail), and sheared using Covaris E220 Focused-Ultrasonicator ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Replicates were pooled and concentrated using a sodium acetate/ethanol precipitation with glycogen (0.1 μg/μL final concentration, Roche, Basel, Switzerland). High molecular weight DNA was confirmed via gel electrophoresis ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Cells were lysed with tissue lysis buffer supplemented with 0.2 mM sodium vanadate and 1 mM protease inhibitor cocktail (Roche, Basel, Switzerland), and for immunoblotting ...
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bioRxiv - Cell Biology 2024Quote: ... 1% w/v sodium deoxycholate and 0.2% w/v NaF] supplemented with protease inhibitors (cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail, Roche, 11836170001). Protein lysates were cleared by centrifugation (16000 g ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mM sodium molybdate) and protease inhibitors (1 mini-Complete EDTA-free tablet per 10 ml lysis buffer; Roche Life Sciences)) and sonicated three times for 15 sec each with intermittent cooling on ice ...
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bioRxiv - Biophysics 2020Quote: ... The lymph nodes were then mechanically disrupted and digested using collagenase D (Roche, Basel, Switzerland) and DNAse1 (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... postnatal and adult hearts were minced and digested in 2.5 mg/mL collagenase D (Roche), 0.25 mg/mL DNase I (Roche ...
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bioRxiv - Immunology 2020Quote: ... The lungs were removed and infiltrated with 2 mg/ml collagenase D (Roche, Indianapolis, Indiana) and 0.2 mg/ml DNase I (Roche ...
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bioRxiv - Immunology 2021Quote: ... Livers and lungs were treated with collagenase D (for lung digestion, 1 mg/ml, Roche) or collagenase Type 4 (for liver digestion ...
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bioRxiv - Immunology 2020Quote: ... secondary lymphoid tissues were harvested and incubated with 10 mg/ml Collagenase D (Roche #11088866001) in plain RPMI medium (Gibco #11875093 ...
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bioRxiv - Neuroscience 2023Quote: ... half hemispheres were digested in a 0.4 mg/ml collagenase D solution (Roche Diagnostics, Germany) in phenol-free RPMI media supplemented with 10 mM HEPES and 5% FBS for 30 min at 37°C ...
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bioRxiv - Immunology 2024Quote: ... Kidney tissue was enzymatically digested with collagenase D at 0.4 mg/ml (Roche, Mannheim, Germany) and DNase I (10 μg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... brains and spleens from Cx3CR1ATGL WT or KO mice were digested with Collagenase D (Roche) and DNase I ...
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bioRxiv - Immunology 2023Quote: ... tissues were minced and enzymatically dissociated with 1 mg/mL collagenase D (Roche Applied Science) and 25 U/mL DNase I (Fujifilm Wako ...
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bioRxiv - Molecular Biology 2024Quote: ... Reverse transcription was performed using oligo-d(T) (IDT) and random hexamer (Roche Diagnostics GmbH) using an enzyme provided by the CRG Protein Technologies Unit in 5X First-Strand Buffer (250 mM Tris-HCl pH 8.3 ...
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bioRxiv - Microbiology 2024Quote: ... placed in lysing matrix D (1.4-mm ceramic spheres, MagNA Lyser Green Bead tubes, Roche), and homogenised in a FastPrep24 (MP Biomedicals ...
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bioRxiv - Immunology 2024Quote: ... Tissue was then minced and digested with 5.4 U/mL collagenase D (Roche Applied Science), 100 U/mL DNase I (Sigma) ...
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bioRxiv - Immunology 2024Quote: ... Livers were digested overnight at 37°C with shaking with 0.2U/ml collagenase D (Roche) in the presence of 5000U/ml polymyxin B (Merck) ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.5% blocking buffer pH 7.5 (100 mM maleic acid, 150 mM NaCl, 10% Roche blocking reagent #11096176001). Antibody staining was performed in 3% BSA in PBS-Triton (0.1%) ...
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bioRxiv - Systems Biology 2021Quote: ... After neutralizing the samples with HCl the free fatty acids were assayed using a commercial kit (Roche). Concentrations of cholesterol and acetate were measured using commercial kits supplied by Boehringer Mannheim.
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bioRxiv - Microbiology 2020Quote: Automated nucleic acid extraction was performed on the MagNA Pure 96 instrument (Roche Applied Science, the Netherlands) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... washed for 30 min with washing buffer (1 × maleic acid buffer + 0.3% Tween 20; Roche, Catalog# 11585762001), then equilibrated for 3 min in detection buffer (Roche ...
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bioRxiv - Microbiology 2020Quote: ... GISAID: EPI_ISL_413570) obtained from cell culture using the MagNA Pure LC Total Nucleic Acid Isolation Kit (Roche). We determined the slope by linear regression in GraphPad Prism and defined the required levels for PCR efficiency (E ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was extracted using the column-based High Pure Viral Nucleic Acid Kit (Roche, Basel, Switzerland), according to the manufacturer’s instructions ...