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Citations for Roche :
701 - 750 of 10000+ citations for 20 Hydroxyecdysone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Pre-hybridization was done at 68 °C for 1 h and using DIG Northern Blot Starter KIT (12039672910, Roche). DIG-labeled in vitro transcribed lncMtDloop ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 µl protein A/G beads (SantaCruz) in the presence of Complete Protein Inhibitor (Roche) and 40 U RNase inhibitors (Roche ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryo preparation and hybridization was performed as described previously [20] using α-Digoxigenin-AP (Roche) and NBT-BCIP solution (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... we added 30 μl of 20 mg/ml proteinase K (Roche 03 115 828 001). The samples were incubated overnight at 55 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were then digested by addition of 2 μL 20 mg/mL Proteinase K (Roche) and incubation for 2 h at 55° C ...
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bioRxiv - Neuroscience 2021Quote: ... 4 μL of the supernatant were incubated with 20 μg/mL proteinase K (PK; Roche) in a total volume of 22 μL RIPA buffer for 1 hr at 37°C to digest all proteins except for PK-resistant PrPSc ...
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bioRxiv - Neuroscience 2022Quote: ... and 20 μg/ml leupeptin) containing CompleteTM Mini Protease Inhibitor Cocktail (Roche Diagnostics, Mannheim, Germany) by sonication ...
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bioRxiv - Microbiology 2022Quote: ... PUb-NS(1F) or PUb-NS(5F) and 20 µl of X-tremeGENE HP (Roche) and medium was refreshed after 3 hours ...
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bioRxiv - Microbiology 2021Quote: ... and washed 3x with 200 μL CMF-PBS + 0.1% Tween-20 (PBST, Sigma-Roche, UK) followed by 2x with 200 μL CMF-PBS ...
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bioRxiv - Neuroscience 2020Quote: ... 50 mM MgCl2 and 0.1% Tween-20) before developing in BM purple AP substrate (Roche) at 37°C 1h – 4 days ...
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bioRxiv - Microbiology 2020Quote: ... 25 μg/ml gentamicin] containing 75 U/ml collagenase and 20 μg/ml dispase (Roche) with gentle shaking at 37°C until isolated crypts could be observed microscopically ...
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bioRxiv - Biochemistry 2019Quote: ... 20% glycerol) containing 0.5 μM 2-mercaptethanol (Fujifilm Wako) and protease inhibitor (Roche, Basel, Switzerland) and solubilized with 0.6% (w/v ...
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bioRxiv - Cell Biology 2019Quote: ... the tissue was permeabilized by incubation in proteinase-K (20 ng/ml K-03115836001 Roche) for 10 min at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... and the RNA was subsequently precipitated in 2-propanol and 20 μg/μl glycogen (Roche) overnight at −20 °C ...
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bioRxiv - Immunology 2022Quote: ... Cells were digested in RPMI-1640 media containing 20 μg/mL DNase (Roche, Basel, Switzerland) and 1 mg/mL collagenase IV (StemCell Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... tissue samples were homogenized (20 strokes on ice) in TBS with protease inhibitors (Roche, Switzerland) using a Teflon homogenizer ...
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bioRxiv - Microbiology 2023Quote: ... and treated for 30 min at 37°C with 20 U/ml DNase I (Roche). Virus was pelleted through a 25% sucrose cushion ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in 20 ml PBS plus two cOmplete Mini EDTA- free protease inhibitor tablets (Roche) and 25 μg/ml phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Biochemistry 2023Quote: ... 0.02% Tween-20) supplemented with 500 mM NaCl and 1x EDTA-free protease inhibitors (Roche) and stored at −20°C until use ...
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bioRxiv - Neuroscience 2022Quote: ... or 20 μg of fluorescein-labeled probes (see below) for fluorescent detection (Roche, cat. #11685619910). After hybridization ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was pre-incubated with 20 mL DIG Easy Hyb™ Granules solution (Roche) at 42 °C for 1 h in a hybridisation oven (Techne™ Hybridisation Oven) ...
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bioRxiv - Molecular Biology 2021Quote: PCR reactions were performed in triplicate in 384 well plates with SYBR Green (Roche) and specific primers ...
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bioRxiv - Plant Biology 2021Quote: ... Amplification involved cDNA (2 μl, 5 ng/μl) in optical 384-well plates (Roche Light Cycler 480 ...
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bioRxiv - Pathology 2021Quote: Cells were sorted directly into a 96-well plate containing cell lysis buffer (Roche), and frozen immediately until random displacement amplification (RamDA ...
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bioRxiv - Cancer Biology 2022Quote: ... plates were fixed with 2% paraformaldehyde/ 2% sucrose and stained with DAPI (#10236276001, Roche). Plates were photographed with the IN Cell Analyzer 2200 high content analyzer (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... Plates were removed from the magnetic block and 50 μl of elution buffer (Roche) was added to each well and mixed by pipetting up and down 10 times ...
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bioRxiv - Neuroscience 2023Quote: ... The plate was inserted in the Roche LightCycler® 480 (Roche Life Science, Switzerland) and the cycles were defined as follows ...
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bioRxiv - Bioengineering 2023Quote: ... qPCR was performed in 384-well plates using the LightCycler 480 Instrument II (Roche). Target gene expression change was calculated relative to non-targeting controls using the ddCt method.
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bioRxiv - Microbiology 2021Quote: ... 1 µg of total RNA was then reverse-transcribed to cDNA using the iScript cDNA synthesis kit (Biorad; #1708891) (Roche). Diluted cDNAs (1:5 ...
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bioRxiv - Molecular Biology 2020Quote: ... Sequencing libraries were constructed from 1 ng of immunoprecipitated and input DNA using the KAPA Hyper Prep kit (KAPA Biosystems) and NEXTflex ChIP-seq barcodes (Bio Scientific).
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bioRxiv - Developmental Biology 2020Quote: ... Sense and antisense digoxigenin-labeled RNA probes were prepared from a BglII-digest of the pMPX2-1 mouse Pax6 cDNA clone (25) using a DIG RNA labeling kit (Roche). Hybridization and stringent posthybridization wash steps were performed at 65°C.
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bioRxiv - Pharmacology and Toxicology 2019Quote: Antiviral azaBINOL B#24 was assessed for inhibition of HIV-1 reverse transcriptase using a commercially available colorimetric reverse transcriptase assay kit (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... cells were washed with ice-cold DPBS and scraped in ice-cold kit lysis buffer (1 mL/107 cells) containing a cocktail of protease inhibitors (cOmplete, EDTA-free; Roche). Cells were then lysed in a Potter-Elvehjem homogenizer (~50 strokes) ...
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bioRxiv - Immunology 2021Quote: Strand-specific ribosomal RNA depleted RNA-seq libraries were prepared from 1 µg of total RNA using the KAPA Stranded RNA-seq Kit with Ribo-Erase (Roche). Samples were prepared in at least triplicate for analysis ...
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bioRxiv - Neuroscience 2020Quote: ... All qPCR reactions were performed in 10 μl volume in triplicates with 1× LightCycler 480 SYBR Green I Master kit (Roche) and primers listed in Supplementary Table 1 on LightCycler® 480 PCR instrument II (Roche) ...
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bioRxiv - Developmental Biology 2022Quote: ... TUNEL staining was performed using ApopTag Red In Situ Apoptosis Detection Kit (S7100, MerckMillipore) combined with peroxidase (POD) coupled anti-DIG antibody (1/200, #11207733910, Roche) followed by tyramide-based amplification61.
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bioRxiv - Immunology 2022Quote: ... Yield of RNA yield was quantitated by nano-drop and 1 µg of RNA was use to reverse-transcribed to cDNA using the iScript cDNA synthesis kit (BIORAD; #1708891) (Roche). 1:5 diluted cDNAs was used for qPCR by using KAPA SYBR® FAST qPCR Master Mix (5X ...
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bioRxiv - Cancer Biology 2021Quote: ... RNAseq libraries were prepared from 1 μg of total RNA per sample using the KAPA Stranded mRNA Sequencing Kit (Roche). In short ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was quantitated by NanoDrop and 1 µg of total RNA was then reverse-transcribed to cDNA using the iScript cDNA synthesis kit (Biorad; #1708891) (Roche). Diluted cDNAs (1:5 ...
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bioRxiv - Cell Biology 2022Quote: ... the RNA concentration was determined and 1 µg RNA was used for the cDNA synthesis according to the Transcriptor First Strand cDNA Synthesis kit (Roche). The expression of the following genes FTSH7 (AT3G47060) ...
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bioRxiv - Physiology 2019Quote: ... sections were incubated for 1 hrs at 37°C in the dark with the in situ Cell Death Detection Kit (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Exon 1 and exon 3 digestion products were purified using the High pure PCR product purification kit (Roche, Basel, Switzerland) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... 1 µg of total RNA was then reverse-transcribed to cDNA using the iScript cDNA synthesis kit (Biorad; #1708891) (Roche). Diluted cDNAs (1:5 ...
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bioRxiv - Microbiology 2021Quote: ... Radiolabeled probe was generated by purifying a digested (Ncol; 1 cut) pCpG vector DNA using High Pure PCR product Purification Kit (Roche) followed by radiolabeling the probe using Random Prime DNA labeling kit (Roche) ...
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bioRxiv - Microbiology 2022Quote: ... lacking the predicted signal peptide (amino acids 1-29) was amplified from genomic DNA (isolated using the High Pure Template kit, Roche) by PCR using the Phusion Hot start II High Fidelity DNA Polymerase (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μg of total RNA from each sample was converted into cDNA with First Strand cDNA Synthesis Kit (Roche, #04897030001). Primers used in the RT-qPCR are listed in Supplementary Table S1 ...
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bioRxiv - Evolutionary Biology 2023Quote: Antisense probes were synthesized using purified PCR products (1 μg per reaction) (supplementary table S16) and DIG RNA labeling Kit (T7) (Roche), following the manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... ChIP-seq libraries were prepared by Duke University Sequencing and Genomic Technologies Core Facility (Duke Sequencing Facility) from 1 ng of ChIP DNA using the KAPA HyperPrep Library Kit (Roche) to generate Illumina-compatible libraries ...
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bioRxiv - Genomics 2022Quote: ... RNA-seq libraries were prepared using 1 μg of total RNA input using KAPA stranded mRNA-seq kit for Illumina (Roche) as per manufacturer’s instruction and paired-end sequenced on NovaSeq 6000 (Illumina).
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bioRxiv - Developmental Biology 2023Quote: RNA sequencing (RNAseq) libraries were prepared using 1 µg of total RNA using the KAPA stranded mRNA-seq kit (Roche) according to the manufacturer’s specifications ...