Labshake search
Citations for Roche :
701 - 750 of 8213 citations for 2 Chloro 1 1 trifluoromethyl 1 3 4 9 tetrahydro 2H beta carbolin 2 yl ethan 1 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... in a 1:1 ratio and added to white 384 well plates (Roche). Plates were run on a 45-cycle protocol using the LC 480 II system (Roche).
-
bioRxiv - Cell Biology 2024Quote: ... 1 mM Na3VO4 and 1 mM NaF) supplemented with protease inhibitor cocktail (Roche) and incubated on ice for 30-60 min ...
-
bioRxiv - Microbiology 2020Quote: ... G355-5 cells were transfected with 1 to 6 µg DNA in 6 cm dishes using X-treameGENE 9 (Roche, Basel, Switzerland). The 293T/17 cell line was obtained from ATCC (CRL-11268 ...
-
bioRxiv - Developmental Biology 2022Quote: ... 25mM NaCl, 1 mM EGTA, 1.5 mM MgCl2, 1 mM DTT, 1% Triton TX-100, 10% Glycerol, 1X Roche Complete protease inhibitors) during 1h at 4°C ...
-
bioRxiv - Cancer Biology 2021Quote: ... Tumor tissues were minced into 1×1 mm fragments and enzymatically dissociated in HBSS containing 1 mg/ml collagenase A (#11088793001; Roche, Basel, Switzerland) and 1 μg/ml DNase I (#07900 ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cell pellets were lysed in 1500 μl ice cold RIPA buffer (50 mM Tris-Cl pH 7.4, 150 mM NaCl, 1% NP40, 0.5% Na-deoxycholate, 0.1% SDS, 1 mM EDTA, Roche cOmplete and 1 mM PMSF). Cell lysates were clarified by centrifugation (13000 x g at 4°C for 10 min ...
-
bioRxiv - Developmental Biology 2024Quote: ... 1 mM EDTA) supplemented with 1 mM PMSF and 1 tablet of cOmplete Protease inhibitor (Roche, cat. No. 11 873 580 001) per 50 ml using a MP Biomedical Fast-Prep-24 5G bead beater ...
-
bioRxiv - Neuroscience 2024Quote: ... volume of lysis buffer (25 mM HEPES pH 7.5, 200 mM NaCl, 1 μg ml−1 benzonase, 1 mM PMSF and Roche protease inhibitor tablets). Cells were lysed by dounce homogenization and the NALCN complex was subsequently solubilized by addition of 2% (w/v ...
-
bioRxiv - Neuroscience 2024Quote: ... volume of lysis buffer (25 mM HEPES pH 7.5, 150 mM NaCl, 1 μg ml−1 benzonase, 1 mM PMSF and Roche protease inhibitor tablets). Cells were lysed by dounce homogenization and the NALCN complex was subsequently solubilized by addition of 2% (w/v ...
-
bioRxiv - Plant Biology 2024Quote: ... The resulting pellet was washed four times with buffer II (10 mM Tris–HCl pH 8.0, 10 mM MgCl2, 0.25 M sucrose, 1% triton X-100, 1 mM DTT, 0.1mM PMSF, 1% Roche protease inhibitor cocktail tablet) until the green color was completely removed ...
-
bioRxiv - Biochemistry 2024Quote: ... was washed once with 80 mL of lysis buffer 1 (20 mM Tris-HCl, pH 8.0) containing 2 mL of EDTA-free protease inhibitor mixture (Roche Applied Science, Penzberg, Germany). Cells were disrupted by passing the cell suspension through a Constant Cell disruption system (TS benchtop ...
-
bioRxiv - Cell Biology 2024Quote: Four mL of nuclear lysate from suspension culture of HeLa cells at protein concentration of 2.5 mg/mL were prepared in buffer (50 mM HEPES, pH 7.4, 150 mM NaCl, 2 mM MgCl2, 1 mM DTT, cOmplete (La Roche Ltd., Basel, Switzerland, 05056489001) and PhosStop RNase inhibitors (La Roche Ltd. ...
-
bioRxiv - Cell Biology 2024Quote: Two mL of nuclear lysate from suspension culture of HeLa cells at protein concentration of 2.5 mg/mL were prepared in buffer (50 mM HEPES, pH 7.4, 150 mM NaCl, 2 mM MgCl2, 1 mM DTT, cOmplete (La Roche Ltd., Basel, Switzerland,05056489001) and PhosStop RNase inhibitors (La Roche Ltd. ...
-
bioRxiv - Cell Biology 2024Quote: Four mL of nuclear lysate from suspension culture of HeLa cells at protein concentration 2.5 mg/mL were prepared in buffer (50 mM HEPES, pH 7.4, 150 mM NaCl, 2 mM MgCl2, 1 mM DTT, cOmplete (La Roche Ltd., Basel, Switzerland, 05056489001) and PhosStop RNase inhibitors (La Roche Ltd. ...
-
bioRxiv - Neuroscience 2020Quote: ... Cells were incubated overnight at 4 °C with rat anti-HA antibody (1:500 - Roche) and then with secondary goat anti-rat IgG antibody coupled to Alexa 568 (Invitrogen/Life Technologies ...
-
bioRxiv - Neuroscience 2024Quote: ... incubated with anti-DIG-POD antibody (Roche; 1:1000 in TNB; 12 hr, 4 °C), and washed in TNT (3 × 20 min ...
-
bioRxiv - Cell Biology 2024Quote: ... Sections were incubated overnight at 4°C with Anti-Digoxigenin-AP antibody (1:2000, Roche) in blocking solution ...
-
bioRxiv - Developmental Biology 2024Quote: ... embryos were incubated overnight at 4°C with anti-digoxigenin antibody (1:5000, Roche 11093274910). Following several washes to remove excess antibodies ...
-
bioRxiv - Microbiology 2023Quote: ... and 0.1% Triton X-100 supplemented with 1 mM PMSF and one Complete Protease Inhibitor tablet (EDTA-free; Roche), and then centrifuged at 13,000 × g for 10 min ...
-
bioRxiv - Systems Biology 2023Quote: ... 500 mM NaCl and 3 mM Dithiothreitol (DTT)) supplemented with 1 mg deoxyribonuclease I (DNaseI, Roche) and protease inhibitor 4-(2-Aminoethyl ...
-
bioRxiv - Cell Biology 2024Quote: ... with primer set #3 (Supplementary Table 1) using KAPA High-Fidelity DNA Polymerase (KAPA Biosystems, KE2502). The forward and reverse primers contained BspEI and KpnII sites respectively ...
-
Dual MAPK and HDAC inhibition rewires the apoptotic rheostat to trigger colorectal cancer cell deathbioRxiv - Cancer Biology 2021Quote: ... 1% NP-40, 0.5% sodium deoxycholate, 1 mM EDTA pH 8 and 1 tablet of protease inhibitor cocktail Roche cOmplete (Roche, Basel, Switzerland) and 1 tablet of phosphatase inhibitor PhosSTOP (Roche)) ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were incubated with the primary antibodies diluted in blocking solution (H3K9me3: ab8898, 1:500, HP1α: CST #2616, 1:200, HA: Roche 3F10, 1:250) for 1h at room temperature ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cell lysates were prepared by incubating cells in RIPA lysis buffer (25 mM Tris pH 7.6, 150 mM NaCl, 1% NP40, 1% deoxycholate, 0.1% SDS, 1X protease inhibitor cocktail from Roche and 1 mM of PMSF) on ice for 30 minutes and cell lysate was clarified by centrifugation at high speed (15 000 rpm ...
-
bioRxiv - Physiology 2022Quote: ... cell pellets were resuspended in RIPA Buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1% IGEPAL CA-630, 1% deoxycholate, 1 mM DTT, DNase, and Roche cOmplete Protease Inhibitor Cocktail) and lysed via sonicating water bath ...
-
bioRxiv - Microbiology 2023Quote: ... 1% NP-40, 1 mM EDTA, 1 mM EGTA, 0.1% SDS, 1X EDTA-free protease inhibitor cocktail [#04693132001, Roche], and 0.5% sodium deoxycholate) and collected using a cell scraper ...
-
bioRxiv - Plant Biology 2023Quote: ... pH 7.5, 150 mM NaCl, 1 mM EDTA, 10 % [v/v] glycerol, 1 mM DTT, and 1 × complete Protease Inhibitor [Roche; Cat. No. 058929700001]) was added to homogenised plant material (100 μl extraction buffer per 100 mg plant material) ...
-
bioRxiv - Biochemistry 2023Quote: ... 0.2% Triton X-100, 1 mM EDTA, 1 mM dithiothreitol, 1 mM NaF, 10 mM β-glycerophosphate, 0.1 mM Na3VO4, 1x Roche cOmplete protease inhibitors). Protein lysates (12 µg per lane ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were subsequently lysed (1 mM NaF, 1 mM Na3VO4, 10 nM calyculin A, 1 mM PMSF, 1 mg complete EDTA-free protease inhibitor cocktail [Roche] in RIPA buffer), scraped ...
-
bioRxiv - Biochemistry 2022Quote: ... 1% NP-40, 150 mM NaCl, 1 mM EDTA pH 8.0, 1 mM EGTA pH 8.0 and Roche complete protease inhibitor EDTA-free) and acid-washed glass beads using a Precellys machine (6 × 30 seconds ...
-
bioRxiv - Cell Biology 2022Quote: ... 1% SDS, 1 mM EDTA, 1 mM DTT, 1 mM Na3VO4×2H2O, 5 µM pepstatin A, 10 µM leupeptin, 2X Roche protease inhibitor cocktail), 200µl glass beads were added ...
-
bioRxiv - Genetics 2022Quote: ... 5 mM EDTA. 0.2% Triton X-100, 1 mM PMSF, 1% Glycerol, 1 pellet/50 ml EDTA free Protease inhibitor [Roche] and 25 μM MG132). 100 μl diluted mixture was used as input ...
-
bioRxiv - Neuroscience 2024Quote: ... rat anti-Elav (1:200) (DSHB), mouse anti-β-galactosidase (1:200) (DSHB), Rabbit anti-Wallenda (1:100, (5)) mouse anti-GFP (1:200, Roche, Basel, Switzerland), Rabbit anti-Ref(2)P (1:200 ...
-
bioRxiv - Physiology 2020Quote: ... Proteins were extracted using 1:1 Pierce RIPA solution with protease inhibitor tablet (Roche). Samples were vortexed for 5 seconds ...
-
bioRxiv - Plant Biology 2021Quote: ... PMSF 1 mM and 1 protease inhibitor tablet (Roche cOmplete EDTA free cat# 05056489001)/50 ml) ...
-
bioRxiv - Microbiology 2020Quote: ... 1% Triton X-100) containing 1 tablet/50 ml of protease inhibitor cocktail (Roche), transferred to a tube and incubated for 15 min at 4°C on a wheel ...
-
bioRxiv - Cell Biology 2022Quote: ... from BD Biosciences, mAb anti-HA-tag (11867423001; IF: 1/500, WB: 1/2000) from Roche, mAb anti-mRFP/DsRed2 (sc-101526 ...
-
bioRxiv - Cancer Biology 2022Quote: ... 1 x protease inhibitor cocktail (cOmplete ULTRA Tablets, Mini, EDTA-free, Roche; 1:10), pH 8.5 ...
-
bioRxiv - Microbiology 2021Quote: ... incubating in 1% blocking solution with 1:10,000 α-Digoxigenin-AP Fab fragments (Roche) for 30 min ...
-
bioRxiv - Cancer Biology 2020Quote: ... mouse anti-GFP (Cat no. 11814460001, 1:300 PLA, 1:4000 WB, Roche Diagnostics), mouse anti-Tubulin (Cat no ...
-
bioRxiv - Neuroscience 2021Quote: ... 10 µg/ml-1 DNase I and 1 protease inhibitors (cOmplete EDTA-free, Roche) for each gram of pellet ...
-
bioRxiv - Systems Biology 2023Quote: ... rabbit anti-furin antibody (1:800, 18413-1-AP, proteintech) and DAPI (10236276001, Roche). The bright-field and immunofluorescent images were acquired using a Pannoramic MIDI system (3DHISTECH ...
-
bioRxiv - Physiology 2024Quote: ... and 1 Na3VO4 and 1% Triton X-100 with PhosSTOP inhibitor tablet (Roche; 4906845001) and protease inhibitor cocktail (Sigma Aldrich ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1 mM PMSF and 1× EDTA-free cOmplete protease inhibitor cocktail (Roche Applied Science). The protein concentration was determined via BCA assay ...
-
bioRxiv - Microbiology 2023Quote: ... 1 mM dithiothreitol which was further supplemented with 1 protease inhibitor cocktail tablet (Roche) and 5 mg RNase A ...
-
bioRxiv - Cancer Biology 2022Quote: ... 1% NP40 supplemented with 1 tablet of protease inhibitor (Complete ultra mini-tablet, Roche) and phosphatase inhibitor (PhosphoStop tablet ...
-
bioRxiv - Plant Biology 2022Quote: ... The following antibodies were used: anti-GFP (1:1000-1:4000, mouse, Roche diagnostics), anti-RFP (1:1000 ...
-
bioRxiv - Microbiology 2022Quote: ... 10U mL-1 DNase 1 and protease inhibitors – Complete Protease Inhibitor Cocktail Tablet (Roche)) ...
-
bioRxiv - Microbiology 2022Quote: ... 10U mL-1 DNase 1 and protease inhibitors – Complete Protease Inhibitor Cocktail Tablet (Roche)) ...
-
bioRxiv - Biochemistry 2022Quote: ... 0.1% Triton X 100 and 1 mM DTT) supplemented with 1× PhosSTOP (Roche, Switzerland), centrifuged at 440 × g for 5 min at 4° C ...