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Citations for Roche :
651 - 700 of 3706 citations for ssc mir 17 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... RT-qPCR reactions were performed on a LightCycler 96 (Roche, Mannheim, Germany) using qPCRBIO SyGreen Mix (PCR Biosystems ...
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bioRxiv - Plant Biology 2023Quote: ... RT-qPCR was performed using LightCycler 480II Real Time system (Roche, USA) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-qPCR was run on a LightCycler 480 II (Roche Applied Science) using 384 well plates (see RT-qPCR section for thermocycling details) ...
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bioRxiv - Cancer Biology 2023Quote: ... RT-qPCR is performed using The LightCycler 480 SYBR Green I (Roche), and the reaction was run at LightCycler 480 Instrument II (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-qPCR was run on a LightCycler 480 II (Roche Applied Science) using 384 well plates ...
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bioRxiv - Synthetic Biology 2023Quote: ... We measured RT-qPCR reaction on a LightCycler® 96 Instrument (Roche) using the SYBR-green channel and with cycling conditions as follows ...
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bioRxiv - Synthetic Biology 2023Quote: ... We analysed RT-qPCR reaction using the LightCycler® 96 Software (Roche). We calculated viral genome concentration (Vg/mL ...
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bioRxiv - Physiology 2024Quote: ... RT-qPCR was performed in duplicate with the LightCycler 480 (Roche Diagnostics) instrument using LightCycler 384-well plates with sealing foil (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... These mutant viruses were made using HEK-293/17 cells that were transfected with 6 μg of viral DNA using Xtreme-GENE HP (Roche, Basel, Switzerland) Transfection reagent ...
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bioRxiv - Microbiology 2023Quote: ... HEK293T/17 cells were seeded onto a 6-well plate and then transfected 24 h later using X-tremeGENE HP (Roche, Basel, Switzerland). After transfection (24 h ...
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bioRxiv - Microbiology 2023Quote: ... Virus stocks were made using HEK-293/17 cells that were transfected with 5 μg of viral DNA using X-tremeGENE HP (Roche, Basel, Switzerland) after 48 h incubation ...
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bioRxiv - Genetics 2021Quote: ... PCR reactions with KAPA HiFi HotStart ReadyMix PCR Kit (Kapa Biosystems) were setup with 80ng genomic DNA ...
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bioRxiv - Bioengineering 2024Quote: ... PCR was performed using KAPA2G Robust PCR kits from Kapa Biosystems. The 25 µL reactions used 25 ng of cDNA ...
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bioRxiv - Systems Biology 2020Quote: ... All primers were used together with LightCycler 480 SYBR Green I Master (Roche). Standard procedures of qRT-PCR were used ...
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bioRxiv - Microbiology 2020Quote: ... and cDNA was synthesized with SuperScript II using Oligo dT primers (Roche Diagnostics). Quantitative PCR was performed using previously described gene specific primers in an ABI7300 Real Time PCR system with SYBR Green Master Mix (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... and 800 nM of each primer in a LightCycler 480 System (Roche Diagnostics). RT-qPCR was run in two biological replicates with three technical replicates each and primers are shown in S7 Table ...
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bioRxiv - Cell Biology 2020Quote: ... Primer-probe combinations were selected based on the Universal Probe Library System (Roche) and are listed in Supplementary table 2 ...
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bioRxiv - Cancer Biology 2021Quote: ... and final molarity quantification determined by qPCR with adapter specific primers (Kapa Biosystems) on a Roche Light Cycler ...
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bioRxiv - Neuroscience 2021Quote: ... All primers were used together with LightCycler 480 SYBR Green I Master (Roche). Three reference genes were used for each qRT-PCR analysis (ACTB ...
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bioRxiv - Neuroscience 2021Quote: ... All primers were used together with LightCycler 480 SYBR Green I Master (Roche). Standard procedures of qRT-PCR were used ...
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bioRxiv - Molecular Biology 2022Quote: ... with primers listed in Table S3 on a LightCycler® LC480 instrument (Roche). Primer efficiencies were > 95% and calculated on standard curves generated by a 2-fold dilution series of cDNAs over at least five dilution points measured in triplicate ...
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bioRxiv - Plant Biology 2021Quote: ... with gene-specific primers (Supplementary Table 6) on a LightCycler 480 system (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... and specific primers near the ends as recommended by the manufacturer (Roche, Germany). Sequence comparisons ...
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bioRxiv - Developmental Biology 2021Quote: ... with specific primers listed in Supplementary Table 1 and a LightCycler 480 (Roche). Samples were run in triplicate and gene of interest expression was normalized to human Gapdh or mouse Rplp0.
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bioRxiv - Genetics 2020Quote: ... All primers were used together with LightCycler 480 SYBR Green I Master (Roche). For mitochondrial analysis we used previously published primers used for mitochondrial copy number assessment (MtDNA ...
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bioRxiv - Immunology 2020Quote: ... Primers were designed using LightCycler Probe Design Software (version 2.0; Roche Applied Science) or the free online Primer3 software (version 0.4.0 ...
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bioRxiv - Immunology 2022Quote: ... and the primers shown in Table S5 on the Light Cycler 480II (Roche) and gene expression was determined and normalized to Hprt using the 2-ΔCt method.
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bioRxiv - Developmental Biology 2022Quote: ... including T7 promoters in the primers and subsequent transcription with T7 Polymerase (Roche). Primer sequences used are found in table 1 ...
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bioRxiv - Molecular Biology 2022Quote: ... using the specific primers and LightCycler-DNA Master SYBR Green I (Roche Diagnostics) or with ABI Prism 7900HT Sequence Detection System (Applied Biosystems ...
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bioRxiv - Molecular Biology 2022Quote: ... using 0.20 uM of primers and KapaSYBR FAST qPCR master mix (Kapa Biosystems).
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bioRxiv - Molecular Biology 2023Quote: ... and 200 nM primers in a LightCycler 96 (Roche Life Science, Basel, Switzerland). Cycling conditions were 95 °C for 3 min ...
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bioRxiv - Systems Biology 2023Quote: ... a qPCR was performed using the KAPA Library Quantification Standards with Primer (Roche Sequencing Solutions Inc ...
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bioRxiv - Developmental Biology 2023Quote: ... Barcoding/adapter primers (Table S7) and KAPA™ HiFi HotStart Ready Mix (Roche) were used to amplify libraries ...
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bioRxiv - Neuroscience 2023Quote: ... Primers were designed using the Universal ProbeLibrary Assay Design Center (Roche, Tab. 2). Transcript levels of candidate genes were measured by qRT-PCR using cDNA with the QuantStudio 6 Flex Real-Time PCR System (Life Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... using a 1:1 mixture of oligo dT and random hexamer primers (Roche). Quantification was performed on a LightCycler 480 (Roche) ...
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bioRxiv - Plant Biology 2020Quote: ... amplification and sequencing was performed by Novogene (Hong Kong) using the SeqCapEZ probe set 140430_Wheat_TGAC_D14_REZ_HX1 for Triticum aestivum (Roche, Nimblegen, WI, USA), described by Krasileva et al ...
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bioRxiv - Genomics 2022Quote: ... Bait sets were amplified in reactions containing 12.5 μl of 2X KAPA HiFi HotStart Polymerase Mix (7958927001, Roche), 0.75 μl of each 10 μM primer ...
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bioRxiv - Genetics 2020Quote: ... PCR products were purified with High Pure PCR Product Purification Kit (Roche). After sequencing reaction ...
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bioRxiv - Cell Biology 2020Quote: ... PCR was performed using KAPA2G Robust Hotstart PCR kit (KAPA Biosystems, #KK5518), following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: ... PCR amplification was performed using KAPA HiFi PCR Mix (Kapa Biosystems KK2602). Specifically ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR was performed with a KAPA HiFi HotStart ReadyMix PCR Kit (Roche) to add Illumina sequencing adapters (P5-dual-index and P7-dual-index ...
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bioRxiv - Bioengineering 2022Quote: ... PCR was performed using 12.5 μl KAPA2G Fast PCR mastermix (KAPA Biosystems), containing reaction buffer ...
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bioRxiv - Immunology 2020Quote: ... qRT-PCR was performed using the LightCycler 480 II PCR System (Roche) with program as followed ...
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bioRxiv - Immunology 2020Quote: ... PCR amplification of the pools with the KAPA HiFi PCR Kit (Roche) was done with 2.5 uL of a 2 ng/uL solution of the pool in a 50uL reaction ...
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bioRxiv - Neuroscience 2019Quote: ... qRT-PCR was performed using the SYBR Green PCR Master Mix (Roche) on a ViiA7 Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Plant Biology 2019Quote: ... Genotyping PCR was performed using KAPA Taq Extra PCR kit (Kapa Biosystems) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2019Quote: ... Quantitative PCR (qRT– PCR) was performed on a 480 LightCycler thermocycler (Roche) using the manufacturer’s instructions with Light cycler 480sybr green I master (Roche ...
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bioRxiv - Microbiology 2021Quote: ... Real-time PCR was carried out using SYBR Green PCR mix (Roche) in Roche LightCycler 480II Instrument.
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bioRxiv - Microbiology 2019Quote: ... A second PCR step using the Expand High Fidelity PCR System (Roche) allowed the incorporation of the Illumina MiSeq version 2 indexes and adapters ...
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bioRxiv - Immunology 2021Quote: ... qRT-PCR was performed using the LightCycler 480 II PCR System (Roche) with the following program ...