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Citations for Roche :
651 - 700 of 1222 citations for Trans 4 Hydroxy L Proline Rabbit Polyclonal Conjugated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... cells were stained with 4′,6-diamidino-2-phenylindole (DAPI) (Roche, 10236276001; 1:10,000) for 5 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... and digested with digestion buffer (Gibco DMEM/F12, Fisher 11320033; 4 µg/ml Roche Collagenase/Dispase ...
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bioRxiv - Developmental Biology 2024Quote: ... 4 h in 1:1500 solution of anti-digoxigenin antibody (Roche, cat no. 11333089001):blocking solution ...
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bioRxiv - Neuroscience 2024Quote: ... Tissues were blocked in blocking buffer comprised of 4% Bovine Serum Albumin (Roche, 10735087001) and 1% Triton X-100 in 1X PBS for 30 minutes at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were hybridized with 30μg/mL of Digoxigenin (DIG) conjugated telomere probe (CCCTAACCCTAACCCTAACCCTAA-DIG, IDT) in DIG Easy Hyb buffer (Roche) overnight at 42°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... tissue was incubated with POD (horseradish peroxidase) conjugated anti-DIG or anti-FLR secondary antibodies (1:100 dilution, Roche Diagnostics ...
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bioRxiv - Developmental Biology 2020Quote: ... they were hybridized overnight at 65°C for three nights and incubated overnight with 1/2,000 alkaline-phosphatase conjugated with anti-DIG Fab fragment (Roche) at 4°C for three nights ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was incubated with horseradish peroxidase (HRP)-conjugated rat anti-HA (clone 3F10) monoclonal antibodies (Roche, Indianapolis, IN) at a dilution of 1:5,000 for 2 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... Signals were detected using anti-mouse secondary antibodies conjugated with alkaline phosphatase using BCIP/NBT substrates (Roche Biochemical, Mannheim). Similarly ...
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bioRxiv - Neuroscience 2020Quote: ... The signals were visualized with a nonradioactive detection system using anti-digoxigenin Fab fragment conjugated to alkaline phosphatase (Roche).
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bioRxiv - Neuroscience 2020Quote: ... the sections were incubated with an anti-DIG antibody conjugated with horse radish peroxidase (HRP) (1/500, Roche Diagnostics) for 6 hours at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... In situ hybridization signals in whole-mount embryos were detected using anti-DIG alkaline phosphatase (AP)-conjugated antibody (Roche) with an AP substrate ...
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bioRxiv - Developmental Biology 2021Quote: ... The mRNA expression patterns were visualized by immunoreactivity with anti-digoxigenin horseradish peroxidase-conjugated Fab-fragments (Roche, Basel, Switzerland), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... ISH detection was performed using anti-DIG secondary antibody conjugated with radish peroxidase (POD) (Roche Diagnostics Corp., Indianapolis, IN) and Opal 570 (1:500 ...
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Noradrenergic alpha-2A receptor activation suppresses courtship vocalization in male Japanese quail.bioRxiv - Animal Behavior and Cognition 2021Quote: ... The slides were incubated overnight with a 1:5,000 dilution of alkaline phosphatase-conjugated anti-DIG antibody (Roche Diagnostics). After the slides had been washed in in a solution of 0.1 M Tris (pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: TUNEL assay (90) was performed using an in situ cell death detection kit conjugated with fluorescein isothiocyanate (Roche, 11684795910). DAPI (Vectashield Antifade Mounting Medium with DAPI ...
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bioRxiv - Molecular Biology 2020Quote: ... The sections were then rinsed with 2× SSC and 0.2× SSC and treated with alkaline phosphatase– conjugated anti-digoxigenin (anti-DIG, 1:500; Roche) for 2 h at room temperature ...
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bioRxiv - Cell Biology 2021Quote: TUNEL assay [47] was performed using an in situ cell death detection kit conjugated with fluorescein isothiocyanate (Roche, 11684795910). DAPI (Vectashield Antifade Mounting Medium with DAPI ...
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bioRxiv - Developmental Biology 2021Quote: ... 20% heat-inactivated lamb serum in Tris-buffered saline with 0.1% Tween-20 (TBST) and incubated with alkaline phosphate-conjugated anti-DIG antibody (1:2000, Roche) in blocking buffer overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... each fatty acid was first conjugated to 10% fatty-acid-free BSA (bovine serum albumin fraction V) (#10735086001, Roche) for 1 hour at 50°C in a 50:50 volumetric ratio ...
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bioRxiv - Molecular Biology 2023Quote: ... Detection was performed using alkaline phosphatase-conjugated anti-digoxigenin Fab fragments and the chemiluminescent substrate CSPD (Roche Applied Science). ImageJ analysis was performed to calculate the relative intensity of Maxi-probe detected bands with the corresponding control-probe detected bands.
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bioRxiv - Molecular Biology 2023Quote: ... isolated cell wall proteins were probed with anti-HA peroxidase-conjugated antibody (1:1,250 dilution; 11667475001, Roche, Basel, Switzerland).
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated with horseradish peroxidase (HRP)-conjugated anti-DIG (Roche Applied Science cat#11207733910, 1:500) and anti-GFP (Aves Labs cat#GFP-1010 ...
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bioRxiv - Molecular Biology 2021Quote: ... Library Enrichment was performed in a 30 μl reaction volume containing 20 μl DNA from the previous step and the following final concentrations: 1X KAPA buffer (Roche #KK2502), 0.3 mM each dNTP (Roche #KK1017) ...
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bioRxiv - Cancer Biology 2019Quote: ... The nuclear pellet was brought up to 880 μL in Nuclear Lysis Buffer (50 mM Tris-HCl pH 7.5, 10 mM EDTA, 0.5% SDS, 1× Roche protease inhibitors, 11697498001), and sonicated with a Bioruptor 300 (Diagenode ...
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bioRxiv - Molecular Biology 2019Quote: ... The nuclear pellet was brought up to 880 µL in Nuclear Lysis Buffer (50 mM Tris-HCl pH 7.5, 10 mM EDTA, 0.5% SDS, 1x Roche protease inhibitors, 11697498001), and sonicated with a Bioruptor 300 (Diagenode ...
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bioRxiv - Molecular Biology 2021Quote: 200 μL of eluate was extracted on the Magna Pure 96 using a DNA and Viral NA Small Volume Kit (Roche, 06543588001) with the universal small volume protocol and eluted into 50 μL proprietary elution buffer ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Animals were then re-suspended on ice in 50 µl of RIPA buffer with added phosphatase inhibitors (Cell Signalling Technologies #9806) and complete EDTA-free protease inhibitor cocktail (Roche # 04693159001) before sonication ...
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bioRxiv - Microbiology 2021Quote: ... samples were enzymatically analyzed using a D-Lactic Acid/L-Lactic Acid Enzymatic Bioanalysis UV-Test kit and a D-Glucose Enzymatic Bioanalysis UV-Test kit (Roche Diagnostics) with a modified manufacturer’s protocol to reduce total sample size to 300 uL ...
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bioRxiv - Microbiology 2021Quote: ... 0.2 pmoles of gene-specific forward primer and 0.2 pmoles of gene-specific reverse primer was combined with 2 µL of cDNA template into each well of a 96-well plate (LightCycler® 480 Multiwell Plate 96, white, 04729692001, Roche). qPCR amplification was performed using the following cycling conditions ...
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bioRxiv - Plant Biology 2021Quote: ... The obtained library was amplified by emulsion polymerase chain reaction (PCR) using a GS FLK Titanium SV/LV emPCR Kit (Lib-L; Roche Diagnostics), added to a GS FLK Titanium PicoTiterPlate (Roche Diagnostics) ...
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bioRxiv - Plant Biology 2020Quote: ... and 6.8 μL ddH2O were used and the RT-qPCR was performed using a Light Cycler 96 Real-Time PCR Machine (Roche, Germany). Expression levels were calculated using the 2-ΔCT equation (Kilambi et al. ...
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bioRxiv - Biochemistry 2022Quote: ... and subsequent addition of 50 µl lysis buffer supplemented with 1 mM PMSF / ml buffer and 22 µl / ml buffer protease inhibitor cocktail (Complete, Roche Diagnostics). The cell samples were boiled for 3 minutes ...
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bioRxiv - Microbiology 2021Quote: ... Ten nanograms of cDNA were used as a template in a 5- l reaction mixture from a KAPA SYBR FAST qPCR kit (Kapa Biosystems). Primers used are listed in table S3 ...
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bioRxiv - Molecular Biology 2021Quote: ... Library Enrichment was performed in a 30 μl reaction volume containing 20 μl DNA from the previous step and the following final concentrations: 1X KAPA buffer (Roche #KK2502), 0.3 mM each dNTP (Roche #KK1017) ...
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bioRxiv - Microbiology 2022Quote: ... incubated overnight at 32°C and replica-plated on a YES agar plate containing 100 mg/L hygromycin B (Roche, #10843555001). The hygromycin plates were incubated for 1-2 days at 32°C until colonies had formed ...
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bioRxiv - Systems Biology 2023Quote: ... medical ventricular drainage and normalization of CSF clinical chemistry analysis (normal CSF protein content lies in the 0.2-0.4 g/L range (Roche et al., 2008)) ...
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bioRxiv - Cell Biology 2022Quote: ... 25 µL of these samples were resolved by SDS-PAGE and analyzed by western blotting with α-HA3 tag antibody (#3F10, Roche) for western blotting ...
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bioRxiv - Genomics 2023Quote: ... Library Enrichment was performed in a 30 μl reaction volume containing 20 μl DNA from the previous step and the following final concentrations: 1X KAPA buffer (Roche #KK2502), 0.3 mM each dNTP (Roche #KK1017) ...
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bioRxiv - Developmental Biology 2023Quote: ... 100 μl of the lysis buffer was added to each 6-well plate along with 1×Protease Inhibitor Cocktail (PIC, Roche cOmplete). The cells were incubated in a rocker at 4°C for 1hr ...
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bioRxiv - Plant Biology 2024Quote: ... The transformants were screened on MS plants with 1% sucrose and 0.8% agar containing either 40mg/L hygromycin (Roche, no. 10843555001) or 25mg/L DL-phosphinothricin (Duchefa ...
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bioRxiv - Pathology 2019Quote: ... Ventana anti-HER2/neu (4B5) rabbit monoclonal primary antibody was purchased from Roche Diagnostics GmbH ...
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bioRxiv - Developmental Biology 2021Quote: ... They are incubated for at least 4 hours in blocking solution 1x WBR (Roche, 11921673001) and incubated overnight at 4°C with anti-Digoxigenin antibody coupled to alkaline phosphatase (Roche ...
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bioRxiv - Molecular Biology 2020Quote: For sequencing all samples were treated with 4 mg/ml of proteinase K (Roche Diagnostics). RNA and small RNA were extracted and enriched in separate fraction from the supernatants using the mirVana kit according to manufacturer’s instructions to separate samples into two groups ...
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bioRxiv - Genomics 2019Quote: All buffers were pre-chilled to 4°C with cOmplete EDTA-free protease inhibitor (Roche) freshly added ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and Nitro Blue tetrazolium/5-bromo-4-chloro-3-indolyl-phosphate NBT/BCIP (Roche Diagnostics). In situ hybridizations were based on the Carroll lab “Drosophila abdominal in situ” protocol (http://carroll.molbio.wisc.edu/methods.html ...
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bioRxiv - Neuroscience 2020Quote: ... sections are incubated with DAPI (4′,6-diamidino-2-phenylindole, Cat. no. 10236276001 Roche, Switzerland) for 10 minutes at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... which were stained with 5-bromo-4-cloro-3-indlyl phosphate/nitro blue tetrazolium (Roche) chromogenic substrates.
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bioRxiv - Neuroscience 2021Quote: ... 4 μL of the supernatant were incubated with 20 μg/mL proteinase K (PK; Roche) in a total volume of 22 μL RIPA buffer for 1 hr at 37°C to digest all proteins except for PK-resistant PrPSc ...
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bioRxiv - Developmental Biology 2020Quote: ... in the presence of nitro blue tetrazolium/5-bromo-4-chloro-3′-indolyphosphate substrates (Roche). Stained embryos and gonads were embedded in gelatin ...