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Citations for Roche :
651 - 700 of 1191 citations for Recombinant Rat Fc Fragment Of LgG Low Affinity IIb Receptor CD32 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... membranes were probed with rat anti-HA (clone 3F10, Roche; 1:1,000) followed by 1:20,000 IRDye 680RD Goat anti-Rat IgG secondary antibody (LI-COR Biosciences) ...
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bioRxiv - Physiology 2023Quote: Primary antibodies and their dilutions were: rat monoclonal against HA (Roche #11867423001), 1:1000 ...
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bioRxiv - Neuroscience 2023Quote: The following commercially available antibodies were used: rat-anti-HA (Roche, #11867431001) and rabbit-anti-GAPDH (Cell Signaling #5174) ...
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bioRxiv - Plant Biology 2024Quote: ... After incubation with rat anti-HA:HRP (3F10 clone, Roche [dilution 1:3000]) or mouse anti-Myc:HRP antibodies (9E10 clone ...
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bioRxiv - Cell Biology 2024Quote: The primary antibodies used were rat-anti-HA 3F10 (Roche, 1:25), mouse-anti-RAP1 (2.29 ...
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bioRxiv - Genomics 2023Quote: ... Converted cDNA is quantified using 2x KAPA SYBR Fast Master Mix ROX Low (KAPA Biosystems, KK4620), with primers against upstream RHOXF2B TSS ...
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bioRxiv - Developmental Biology 2022Quote: ... supplemented with 10% FCS (P3 and P9) and treated with 0.05 mg/ml Liberase TH (Roche) and 0.2 mg/ml DNAse (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... NRP2-Fc point mutations were introduced by PCR mutagenesis using Kapa HiFi DNA polymerase (Kapa Biosystems), according to the QuikChange site-directed mutagenesis manual ...
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bioRxiv - Molecular Biology 2020Quote: ... were generated by transfection of 3×FLAG-tagged LRRK2 (WT) plasmid followed by pharmacological selection using an antibiotic G-418 (Roche). Transfection of plasmids and siRNA was performed using Lipofectamine 3000 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... eGFP-tagged readthrough reporter was immunoprecipitated by addition of 10 μg of mouse monoclonal anti-GFP IgG (Roche, cat#11814460001) or normal mouse IgG as a negative control (Proteintech ...
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Evolutionary repair: changes in multiple functional modules allow meiotic cohesin to support mitosisbioRxiv - Evolutionary Biology 2019Quote: ... Rec8 and Scc1 were all tagged with 3xHA at the C-terminus of coding sequences and anti-HA antibody (3F10, Roche) was used to detect the abundance of kleisin proteins ...
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bioRxiv - Molecular Biology 2021Quote: Aag2 cells were transfected with a plasmid expressing 3×flag tagged Zuc using X-tremeGENE HP DNA Transfection Reagent (Roche), and fixed 48 hours after transfection ...
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bioRxiv - Molecular Biology 2021Quote: A 3×flag tagged Zuc expression plasmid was transfected into Aag2 cells using X-tremeGENE HP DNA Transfection Reagent (Roche). Cells were lysed and lysates incubated with M2-Flag beads (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... 293T cells (from four confluent T175 flasks per GST-tagged bait) were collected by scraping and lysed with lysis buffer supplemented with protease inhibitor cocktail (EDTA-free, cOmplete, Roche). The lysate was clarified by centrifugation for 5 minutes at 17,000 x g and pre-cleared with 100 μL of Glutathione Sepharose bead slurry per bait ...
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bioRxiv - Biochemistry 2023Quote: ... or HT alone and 0.001 μg/well N-terminally NL-tagged CCT5 and MAGEA3 (FL or -EE degrons) using X-tremeGene HP transfection reagent (Roche), following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... These DNA molecules were then aligned as the chromium barriers and flow-stretched so that the dig-tagged ends were anchored to chromium pedestals coated with anti-dig antibody (Roche). These DNA molecules were then flow-stretched so that the dig-ends were anchored at chromium pedestals coated with anti-dig antibody (Roche) ...
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ER mediates spatial regulation of lysosome-endosome interactions via motion switch at junction sitesbioRxiv - Cell Biology 2023Quote: ... a total of ∼1×107 HEK293T cells stably expressing the GFP-STARD3 or Flag-tagged YWHAH were harvested and lysed using the lysis buffer supplemented with protease inhibitor (Roche). Clarified lysate was mixed with pre-washed 10 𝜇l of GFP-Trap A beads (Chromotec gta-20 ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were co-transfected with 5 μg of Myc-tagged NAT10 and 5 μg of BRD4 short or long isoform using the Xtremegene 9 DNA transfection reagent (Roche). After overnight incubation ...
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bioRxiv - Molecular Biology 2024Quote: ... GFP-tagged Myh was isolated from cell lysate using Dynabeads Protein G (Thermo 10004D) and GFP-tag Antibody (Roche #11814460001). IP and input were boiled in 5xLaemmli Buffer and separated on an SDS-PAGE (8-16% ...
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bioRxiv - Cell Biology 2019Quote: ... Beads were removed by centrifugation (twice at 1,000 xg) and then pre-cleared lysates were supplemented with anti-HA high affinity monoclonal antibody (clone 3F10, Roche) at 2 µg/ml final concentration along with 20 µl Protein G Sepharose beads and incubated at 4° C for 4 hours on a rotating wheel ...
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bioRxiv - Plant Biology 2021Quote: ... SMXL5-3xHA and 6xMyc-OBE3 bands were detected by antibodies Anti-HA-Peroxidase High Affinity (3F10) (Roche; Basel, Switzerland) or c-Myc Antibody (9E10 ...
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bioRxiv - Biochemistry 2019Quote: ... CaCl2 was added to a final concentration of 1.25 mM and incubated with 200 μl Anti-Protein C Affinity Matrix (Roche) for 4 hrs while rotating at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Primary antibodies were incubated overnight at 4°C: rabbit anti-HA high affinity at 1:500 (Roche Diagnostics #ROAHAHA), mouse anti-FLAG M2 at 1:500 (Sigma-Aldrich #F1804) ...
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bioRxiv - Neuroscience 2023Quote: Recombinant human (rh)EPO (5000 IU/kg body weight; NeoRecormon, Roche, Welwyn Garden City, UK) or placebo (PL ...
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bioRxiv - Biochemistry 2022Quote: ... CrCEP19107-195 were performed by loading the cleared lysate onto an IMAC cOmplete His-Tag 5ml column (Roche) pre-equilibrated in 5 column volumes (CV ...
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bioRxiv - Microbiology 2021Quote: ... Protein was purified from filtered cell supernatants using StrepTactin resin (IBA) or cOmplete His-Tag Purification Resin (Roche) or Jacalin (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... centrifuged and filtered expression medium was loaded onto a prepacked 5 mL cOmpleteTM His-Tag purification column (Roche) equilibrated in HBS ...
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Characterization of epigenetic alterations in esophageal cancer by whole-genome bisulfite sequencingbioRxiv - Cancer Biology 2021Quote: ... DNA fragments were then PCR amplified by HiFi HotStart Uracil and ReadyMix (Kapa Biosystems). The clustering of the index-coded DNA samples was performed by Illumina cBot Cluster Generation System ...
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bioRxiv - Microbiology 2020Quote: ... Libraries were validated using a Fragment Analyzer and quantified by qPCR (ROCHE LightCycler 480).
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bioRxiv - Molecular Biology 2021Quote: ... The purified DNA fragments were prepared with the KAPA HTP Library Preparation kit (Roche) and single-end sequenced with a 65-cycle kit on an Illumina HiSeq 2500.
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bioRxiv - Developmental Biology 2021Quote: ... Samples were treated with alkaline-phosphatide anti-DIG-AP Fab fragments (1:2000, Roche). Signals were developed using 4-nitro blue tetrazolium chloride (NBT ...
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bioRxiv - Developmental Biology 2022Quote: ... The probes were detected with AP coupled with anti-digoxigenin Fab fragments (Roche, 11093274910). AP reaction was developed with 4-Nitro blue tetrazolium chloride (NBT ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Samples were then incubated with anti-digoxigenin Fab fragments conjugated to alkaline phosphatase (Roche) diluted 1:6000 in PBT+BSA ...
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bioRxiv - Genomics 2020Quote: ... and the probe was stained with rhodamine-conjugated anti-digoxigenin Fab fragments (Roche Diagnostics).
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bioRxiv - Neuroscience 2020Quote: ... the following antibodies and tyramide solutions were used: Anti-Fluorescein-POD Fab fragments (Roche), Anti-Digoxigenin-POD Fab fragments (Roche ...
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bioRxiv - Developmental Biology 2022Quote: ... Incubation with a 1:1,500 dilution of the alkaline phosphatase conjugated Fab fragments (Roche Molecular Biochemicals ...
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bioRxiv - Molecular Biology 2020Quote: ... or Anti-Fluorescein-POD antibody (1:100, Anti-Fluorescein-POD, Fab fragments, 11426346910, Roche) was added and the slides were incubated at 4 °C overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... Anti-DIG-POD antibody (1:100 dilute, Anti-Digoxigenin-POD, Fab fragment, 11207733910, Roche) or Anti-Fluorescein-POD antibody (1:100 ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA fragment was amplified by Expand High Fidelity PCR System (Roche, cat. No. 11732650001) using #1101 and #1146 primers and Msm chromosomal DNA ...
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bioRxiv - Microbiology 2021Quote: ... PCR fragments were amplified from genomic DNA using Kapa Hifi DNA polymerase (Kapa Biosystems). PCR fragments were either digested using restriction endonucleases and ligated into the respective plasmid backbone or assembled directly into a linearised plasmid backbone using Gibson assembly (NEBuilder HiFi DNA Assembly ...
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bioRxiv - Genetics 2019Quote: ... Samples were then incubated with anti-digoxigenin Fab fragments conjugated to alkaline phosphatase (Roche) diluted 1:6000 in PBT+BSA ...
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bioRxiv - Molecular Biology 2019Quote: ... labeled with alkaline phosphatase–conjugated sheep anti-DIG FAB fragments (11093274910, Roche, Basel, Switzerland), before visualized with Liquid Permanent Red (DAKO ...
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bioRxiv - Microbiology 2021Quote: ... incubating in 1% blocking solution with 1:10,000 α-Digoxigenin-AP Fab fragments (Roche) for 30 min ...
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bioRxiv - Pathology 2021Quote: Slides were incubated with 1.5 U/ml of anti-DIG-AP Fab fragments (Roche) diluted in 0.1M TRIS buffer (pH 7.5 ...
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bioRxiv - Developmental Biology 2020Quote: ... the slides were immersed in anti-Digoxin-Rhodamine Fab fragments (1 mg/mL; Roche) antibody or anti-Digoxin-AP (Roche ...
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bioRxiv - Neuroscience 2019Quote: ... or IMPA1L 120nt fragment were labeled using Random Priming Labeling Kits (Takara or Roche) and [α−32P] dCTP ...
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bioRxiv - Microbiology 2019Quote: ... the DNA fragments were transferred to a positively charged nylon membrane (Roche, Mannheim, Germany) and visualized according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2021Quote: ... Histone-associated DNA-fragment levels were analyzed using the Cell Death Detection ELISAPLUS (Roche; Cat ...
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bioRxiv - Synthetic Biology 2022Quote: ... and quality control was performed via fragment analyzer (BioAnalyzer) and qPCR (Roche Lightcycler 480). The final library pool was normalized ...
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bioRxiv - Cancer Biology 2022Quote: ... The purified DNA fragments were prepared using the KAPA HTP Library Preparation Kit (Roche) following manufacturer’s instructions ...