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Citations for Roche :
651 - 700 of 801 citations for Recombinant Human CDH8 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: Proteins were extracted using Tris-buffered Saline (TBS) with 0.5% NP40 protein extraction buffer containing protease and phosphatase inhibitors (Roche), on ice ...
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bioRxiv - Biochemistry 2019Quote: ... CaCl2 was added to a final concentration of 1.25 mM and incubated with 200 μl Anti-Protein C Affinity Matrix (Roche) for 4 hrs while rotating at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... Whole cell extracts were prepared after transfection and incubated with indicated antibodies together with Protein A/G beads (Roche) overnight ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were harvested and ruptured with a Dounce homogenizer in the buffer A containing cOmplete protein inhibitor cocktail (Roche) and 1mM phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were obtained by lysing cells in PBS containing 0.1% Triton X-100 (PBS/TX) as well as protease and protein inhibitor cocktails (Roche) for 5 min on ice ...
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bioRxiv - Biochemistry 2022Quote: ... The cell lysate was diluted to 0.8 mg/ml total protein concentration and 0.47% NP40 using dilution buffer (PBS plus protease inhibitor cocktail (Roche 04693159001) and precleared using Invitrogen™ Dynabeads™ MyOne™ Streptavidin T1 magnetic beads for 2-4 hours at 4°C during gentle rotation ...
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bioRxiv - Biochemistry 2022Quote: ... EDTA-free TCA buffer was used where phosphorylation of proteins was to be investigated by alkaline phosphatase-treatment (Roche) or Phos-tagTM (NARD Institute ...
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bioRxiv - Plant Biology 2022Quote: ... and the interaction between AtRsmD and the selected proteins was determined by immunoblot analysis with a mouse anti-GFP monoclonal antibody (1:5,000; Roche) and a mouse anti-Myc monoclonal antibody (1:10,000 ...
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bioRxiv - Neuroscience 2022Quote: ... Lysate was precleared with Pierce™ Protein G Agarose and immunoprecipitated with anti-GFP antibodies (Roche Cat. No. 11814460001) bound on protein G agarose ...
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bioRxiv - Microbiology 2023Quote: ... Expressed proteins in the cell-culture supernatant were purified using a cOmplete His-Tag Purification Resin (Roche, Cat# 5893682001) affinity column ...
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bioRxiv - Neuroscience 2023Quote: ... Protein was eluted with wash buffer containing 10 mM maltose and subsequently bound to c0mplete His tag resin (Roche) for 2 hours ...
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bioRxiv - Cancer Biology 2023Quote: Protein were isolated by using RIPA buffer with protease inhibitor cocktail (cOmplete Protease Inhibitor Cocktail, Roche, Mannheim, Germany, 04693116001), and concentrations were measured using the Qubit Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... isolated cell wall proteins were probed with anti-HA peroxidase-conjugated antibody (1:1,250 dilution; 11667475001, Roche, Basel, Switzerland).
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bioRxiv - Pathology 2023Quote: ... Approximately 30-50 μg of proteins were separated by 10% SDS-PAGE and transferred onto a PVDF membrane (Roche). The membrane was blocked with 5% non-fat milk in PBS for 1 h at room temperature and then incubated with the specific primary antibodies at 4°C overnight ...
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bioRxiv - Plant Biology 2023Quote: ... Meristem-enriched samples or whole seedlings were ground in liquid nitrogen and homogenized in cold protein extraction buffer (50 mM Tris HCl pH 7.5, 10% glycerol, 1 mM DTT, 1% IGEPAL, 1× Roche EDTA-free protease inhibitor and 1× Roche phosphatase inhibitor) ...
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bioRxiv - Cancer Biology 2023Quote: ... Whole cellular protein was extracted with RIPA buffer (Serva electrophoresis) containing protease inhibitor cocktail and phosphatase inhibitor cocktail (Roche). Western blots were performed following routine protocols55 ...
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bioRxiv - Microbiology 2023Quote: ... Expressed proteins in the cell-culture supernatant were purified using a cOmplete His-Tag Purification Resin (Roche, Cat# 5893682001) affinity column ...
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bioRxiv - Cell Biology 2023Quote: ... target proteins were detected by using primary antibodies of mouse monoclonal anti-GFP antibody (1: 2000; Roche, Cat. 11814460001), mouse anti-mCherry (1:5000 ...
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bioRxiv - Biochemistry 2024Quote: ... resuspended in extraction buffer (SDS 2%, 50 mM Tris-Cl pH 7.4, 1 mM PMSF, 2x cOmplete protein inhibitor cocktail (Roche)) ...
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bioRxiv - Cancer Biology 2024Quote: ... or protein extraction (50 mM Tris-HCl, 1% NP40, 0.15 M NaCl, pH 8) supplemented with phosphatase (PhoStop, Roche) and protease inhibitors (Complete ...
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bioRxiv - Cancer Biology 2024Quote: Total proteins were extracted from cultured NB cells using RIPA buffer supplemented with protease and phosphatase inhibitor cocktail (Roche). The cell lysates were pre-cleared with protein A or protein G beads (Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2020Quote: ... The predicted structure was send to the FunFOLD2 server for the prediction of protein–ligand interactions (Roche et al., 2013). In addition ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were lysed after 24 hours and protein was extracted with RIPA buffer with 1x protease inhibitors EDTA-free (Roche). Protein expression was measured through LICOR Western blotting as described above.
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bioRxiv - Cell Biology 2020Quote: ... post-nuclear lysates pre-cleared using CL-4B Sepharose beads followed by immunoprecipitation with anti-HA-antibody (12CA5) and Protein G agarose (Roche). Beadbound radiolabelled substrates were resuspended in 2 x Laemmli buffer (+20 mM DTT) ...
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bioRxiv - Cell Biology 2020Quote: ... The pellets were washed once with Buffer A and then further lysed with 250 μl modified RIPA buffer (1mM EDTA, 150mM NaCl, 0.1% Na-DOC, 1% NP-40, 50Mm Tris pH7.5 and Complete Roche protein inhibitors). 50U of benzonase (nuclease ...
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bioRxiv - Cell Biology 2020Quote: ... generation of total protein from MEF cells was contained by homogenization of cells in lysis buffer containing protease inhibitor (Complete, Roche). For determination of G/F-actin ratio fractionation was performed as described in Huang et al ...
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bioRxiv - Genomics 2021Quote: Reversal of crosslinks and protein degradation: The chromatin samples were incubated with 200 μl proteinase K digestion buffer containing 30 μg Proteinase K (Roche), 0.2 M NaCl and 30 mM Tris-HCl at 65°C for 12 hours.
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bioRxiv - Cell Biology 2022Quote: ... The lysate was clarified for 30min at 15,000xg and the supernatant was immunoprecipitated for 2h at 4°C with Dynabeads-protein G covalently coupled to the rat anti-HA antibody (Roche). The beads were washed 3×5 min ...
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bioRxiv - Neuroscience 2021Quote: Protein samples were extracted from cells using ice-cold RIPA buffer with complete protease inhibitor mixture (Roche Applied Science, 04693124001). For immunoprecipitation ...
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bioRxiv - Molecular Biology 2020Quote: ... Then cells were lysed in B-per protein extraction reagent 4 ml/ gram bacterial pellet in the presence of cOMPLETE mini protease inhibitor cocktail (Roche) for 15 minutes at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... lysate supernatant from cells expressing N-terminal 6-His-tagged proteins were incubated with nickel-nitrilotriacetic acid (Ni-NTA) cOmplete His-tag purification resin (Roche) for 1 h at 4° C ...
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bioRxiv - Cell Biology 2022Quote: ... Lysis buffer was supplemented with protease and protein phosphatase inhibitor tablets (04-693-159-001 and 04-906-845-001 respectively, Roche). Protein content was determined with the BCA Protein Assay kit (23225 ...
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bioRxiv - Developmental Biology 2022Quote: ... Collected tissues were ground in ice-cold protein lysis buffer (Beyotime, Shanghai, China) containing protease inhibitors cocktail (Roche, Basel, Switzerland) for protein extraction ...
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bioRxiv - Biochemistry 2019Quote: ... 50 µl of the supernatant was kept as input and the remaining supernatant was used for immunoprecipitation with the indicated antibody coupled to protein A-Agarose (Roche) or in case of FLAG with anti-FLAG M2 Affinity gel (Sigma Aldrich) ...
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bioRxiv - Molecular Biology 2019Quote: Total proteins were extracted in RIPA buffer containing 1 mM sodium orthovanadate and Complete Protease Inhibitor Cocktail (Roche Applied Science) and quantified using the Pierce™ BCA protein assay kit (Thermo Fisher) ...
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bioRxiv - Plant Biology 2019Quote: ... The presence of immunocomplexes was assessed by probing protein gel blots with either anti-HA (3F10, Sigma/Roche; http://www.sigmaaldrich.com/) or anti-Myc antibody (9E10 ...
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bioRxiv - Cell Biology 2019Quote: For MS analyses immunoprecipitates on Protein-G Dybabeads were washed 2x with TEAB Buffer (100mM) and incubated with Trypsin (Roche) at 37°C for 18 hours ...
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bioRxiv - Neuroscience 2021Quote: ... the lysates were centrifuged (10 min, 20000 g, 4 °C) and the resulting supernatants were added to 25 µl Protein G Agarose (Roche), rotating for 1 hour at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: Cells were lysed with 0.1% Triton in PBS- (phosphate-buffered saline without CaCl2 or MgCl2) supplemented with EDTA-free protein inhibitor cocktail (complete, Roche) on ice ...
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bioRxiv - Cell Biology 2021Quote: ... Protein extracts were clarified by centrifugation and incubated with α-Pk clone SV5-Pk1 (AbSerotec) orα-HA clone 12CA5 (Roche) antibodies for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... MSNs were harvested in M-PER Mammalian Protein Extraction Reagent (Pierce) with cOmplete Mini EDTA-free protease inhibitor (1 tablet/10 mL) (Roche). Whole-cell lysates were sonicated with a 5 second pulse followed by a 5 second rest (X 5 times ...
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bioRxiv - Cancer Biology 2020Quote: Protein extracts were prepared from drug-treated cells by lysis on ice for 20 minutes in M-PER Mammalian Protein Extraction Reagent (ThermoScientific) supplemented with protease and phosphatase inhibitors (Roche). Lysates were centrifuged at 16,000 RPM for 20 minutes at 4°C and the supernatants were assessed for concentration via a Bradford assay ...
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bioRxiv - Microbiology 2020Quote: Further protein extraction steps for each of 10 samples were carried out in solutions supplemented with cOmplete protease inhibitors (Roche) and PhosSTOP phosphatase inhibitor cocktail (Roche) ...
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bioRxiv - Cell Biology 2020Quote: Protein lysates were obtained from tissues or cells using RIPA lysis buffer containing protease and phosphatase inhibitor cocktails (Roche, USA). Western blotting was conducted as described previously (Yao et al. ...
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bioRxiv - Biochemistry 2022Quote: ... The lysed cells were centrifuged for 15 minutes at 13000 rpm and then the supernatants were incubated mouse monoclonal anti-huntingtin antibody MAB2166 (MilliporeSigma) conjugated Protein G Agarose (Roche) at 4°C for 2 hours ...
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bioRxiv - Neuroscience 2022Quote: ... At DIV 17 cells were washed with ice-cold PBS and immediately extracted with ice-cold T-PER protein extraction buffer (Pierce) supplemented with inhibitors for proteases and phosphatases (Roche). The lysates were centrifuged at 15000 g for 15 minutes at 4°C and the supernatants were used for further analysis.
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bioRxiv - Neuroscience 2022Quote: ... Lysates were diluted to a final total soluble protein concentration of 1 mg/ml in TBS containing cOmplete mini protease inhibitor tablet (Roche). For AD ...
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bioRxiv - Neuroscience 2020Quote: Surface expression of HA-C99 and cc-del(X) fusion proteins was measured with monoclonal HA-antibody (Roche 1:100) by flow cytometry as described previously (Staerk et al. ...
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bioRxiv - Plant Biology 2021Quote: Total proteins were isolated from cell cultures in mid-exponential phase in Hepes 50mM supplemented with EDTA-free protein inhibitor cocktail (Roche). Cell walls were broken by three 30-s cycles at 10000 rpm in the presence of micro glass beads (200-400 µm diameter Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... protein extracts were prepared as previously described (Alao et al., 2014) with addition of 1 × PhosStop phosphatase inhibitor cocktail (Roche Diagnostics Scandinavia AB ...