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Citations for Roche :
651 - 700 of 2590 citations for Rabbit Anti Sheep IgG H+L HRP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: 1 μL amplified cDNA library was used as template in a 29-cycle PCR reaction using KAPA HiFi HotStart ReadyMix (Roche). To avoid possible PCR-induced library biases ...
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bioRxiv - Microbiology 2022Quote: ... The solution was incubated for 20 min at 37°C and 15 μl of proteinase K (20 g L-1l; Roche) was added ...
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bioRxiv - Microbiology 2022Quote: ... A cell pellet was resuspended in 250 μL Tris-EDTA buffer and 50 μL 30 g L-1 lysozyme (Roche) in Tris-EDTA buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... the supernatant was removed and the pellet was resuspended in 250 µL of Galbraith buffer pH 7.0 plus 0.1% Triton X-100 and 1x cOmplete Protease Inhibitor Cocktail (Roche, Mannheim, Germany). 50 µL of 0.2 µm glass beads were added and samples were vortexed for 3 minutes to smash open spores and release nuclei ...
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bioRxiv - Molecular Biology 2023Quote: ... 25 μL 1 M spermidine, 500 µL 10% Triton X-100, and 10 ml glycerol in 38 ml dH2O, 1 Roche Complete Protease Inhibitor EDTA-Free (Millipore Sigma 11873580001)) ...
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bioRxiv - Immunology 2023Quote: ... 1 vial dissolved in 5mL HBSS to yield 20U of papain/mL in 1mM L-cysteine with 0.5mM EDTA) and 1mg/mL DNAse I (Roche 10104159001) with curved scissors ...
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bioRxiv - Immunology 2023Quote: ... 0.5 –1 L of the crude lysates were treated with 5 mg/mL each of DNase I and RNAse (Roche) for one hour at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... The construct was synthesized by GenScript® and the plasmid was used as template for PCR amplification (Kapa Hifi Hotstart Ready Mix, Cat#F-530-L, Roche). The PCR amplicon was purified with NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel ...
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bioRxiv - Genomics 2023Quote: ... were put on ice for 15min then resuspended in 250μl chilled douncing buffer (10mM Tris-HCl pH7.5, 4mM MgCl2, 1mM CaCl2) with 1X Protease Inhibitory Cocktail (PIC, Roche 5892791001). Cells were homogenized through a 1ml 25g 5/8 syringe for 20 times and kept on ice ...
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bioRxiv - Biochemistry 2023Quote: ... were used to inoculate each well of a 96-well plate containing 196 μL of LB media (supplemented with ampicillin, and 200uM IPTG) The plate was covered with transparent LightCycler480 Sealing Foil (Roche), and grown for 24 h in a Biotek Synergy H1 plate reader at 42 °C with double orbital shaking ...
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bioRxiv - Neuroscience 2023Quote: ... The cell pellet was resuspended (20 mL/L culture volume) in lysis buffer (20 mM Tris pH 8, 150 mM NaCl, and protease inhibitor cocktail, Roche). These resuspended cells were disrupted using sonication (QSonica sonicator ...
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bioRxiv - Genomics 2024Quote: ... or 3 µl of direct lysis extract were used as template in 50 µl PCR reactions using KAPA HiFi HotStart ReadyMix (Roche), primers P3/4 and P5 (Supplementary Table 2) ...
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bioRxiv - Molecular Biology 2024Quote: ... Adapters were ligated by adding 15 µL of the ligation mix (3 µL T4 Ligase Buffer 10X, 0.5 µL T4 ligase (5 U/µL, Roche, 10799009001), 0.25 µl of x-Gene Stubby Adapter 50 mM (IDT) ...
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bioRxiv - Molecular Biology 2024Quote: ... Ovaries were cut into 6-8 pieces and enzymatically digested in L-15 media containing Liberase TM (Roche, Indianapolis, IN) and DNase I (Worthington Biochemicals ...
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bioRxiv - Plant Biology 2024Quote: ... The transformants were screened on MS plants with 1% sucrose and 0.8% agar containing 40mg/L hygromycin (Roche, no. 10843555001). For each construct ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by incubation (16 h at 4 °C) in the presence of 10 pM TBST containing complete TM protease inhibitor cocktail (Roche Pharma, Basel, Switzerland). The membranes were washed three times with TBS (150 mM NaCl ...
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bioRxiv - Neuroscience 2022Quote: N2a cells were collected for expression validation from the 12-well plate at 24 h post transfection in RIPA buffer (Cell Signalling Technologies; Catalog No. 9806) in the presence of phosphatase inhibitor (Roche; Catalog No. 04906837001) and protease inhibitor (Roche ...
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bioRxiv - Microbiology 2021Quote: 48 h bacterial cultures were pelleted and were resuspended in RIPA buffer containing protease inhibitors cocktail (Roche, Cat. No. 11 836 145 001). Samples were sonicated at 50% voltage for 5 cycles of 10 sec pulses followed by 30 sec rest on ice ...
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bioRxiv - Cell Biology 2020Quote: ... and then digested for 1 h at 37°C in a vigorously shaking solution consisting of 16 mg of Collagenase D (Roche, Pleasanton, CA, USA), 3.0 U of Dispase II (Roche) ...
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bioRxiv - Biochemistry 2021Quote: ... The band corresponding to the crosslinked nanobody-antigen complex was then excised from the gel and subjected to in-gel digestion with trypsin (Roche, 1μg, 4 h) or chymotrypsin (Roche ...
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bioRxiv - Neuroscience 2022Quote: ... 20 mg protein was loaded onto 8-10% SDS-PAGE gels for electrophoresis and subsequently transferred (90 V, 2-3 h) onto PVDF western blotting membranes (Roche, Cat. No. 3010040001) using the Criterion™ System BioRad ...
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bioRxiv - Biophysics 2020Quote: ... Pyruvate kinase (10109045001) from rabbit muscle was purchased from Roche (Basel, Switzerland). Poly-(dimethylsiloxane ...
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bioRxiv - Microbiology 2020Quote: ... Anti-proteases cOmplete and anti-phosphatases PhoSTOP were from Roche. Nigericin was from Sigma ...
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bioRxiv - Microbiology 2022Quote: ... and anti-DIG antibody (Anti-Digoxigenin-AP Fab fragments; Roche), before washing and visualisation ...
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bioRxiv - Genomics 2020Quote: ... anti-GFP (Roche) and anti-L1 ...
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bioRxiv - Microbiology 2020Quote: ... anti-HA (Roche, cat#11867423001 ...
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bioRxiv - Microbiology 2020Quote: ... anti-HA (Roche, cat#11867423001 ...
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bioRxiv - Cell Biology 2019Quote: ... anti HA (Roche), anti-V5 (Invitrogen ...
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bioRxiv - Plant Biology 2021Quote: ... Anti-HA (Roche) conjugated Dynabeads Protein A (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... anti-HA (Roche), anti-GFP (ab6556 ...
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bioRxiv - Cell Biology 2021Quote: ... anti GFP (Roche) 1:1000 and anti-Mouse IgG (whole molecule)–Peroxidase antibody (Sigma ...
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bioRxiv - Plant Biology 2022Quote: ... anti-HA (Roche), anti-Pol II (Ab26721 ...
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bioRxiv - Molecular Biology 2022Quote: ... anti-GFP (Roche, clones 7.1 and 13 ...
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bioRxiv - Microbiology 2023Quote: ... anti-proteases (Roche)) ...
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bioRxiv - Plant Biology 2024Quote: ... anti-GFP (Roche), anti-HA (high affinity clone 3F-10 ...
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bioRxiv - Immunology 2020Quote: ... Roche Elecsys® Anti-SARS-CoV-2 Immunoassay for the qualitative detection of antibodies (including IgG) against SARS-CoV-2 was used with a COBAS e411 analyzer (disk system; Roche, Switzerland) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... Library Enrichment was performed in a 30 μl reaction volume containing 20 μl DNA from the previous step and the following final concentrations: 1X KAPA buffer (Roche #KK2502), 0.3 mM each dNTP (Roche #KK1017) ...
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bioRxiv - Cancer Biology 2019Quote: ... The nuclear pellet was brought up to 880 μL in Nuclear Lysis Buffer (50 mM Tris-HCl pH 7.5, 10 mM EDTA, 0.5% SDS, 1× Roche protease inhibitors, 11697498001), and sonicated with a Bioruptor 300 (Diagenode ...
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bioRxiv - Molecular Biology 2019Quote: ... The nuclear pellet was brought up to 880 µL in Nuclear Lysis Buffer (50 mM Tris-HCl pH 7.5, 10 mM EDTA, 0.5% SDS, 1x Roche protease inhibitors, 11697498001), and sonicated with a Bioruptor 300 (Diagenode ...
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bioRxiv - Molecular Biology 2021Quote: 200 μL of eluate was extracted on the Magna Pure 96 using a DNA and Viral NA Small Volume Kit (Roche, 06543588001) with the universal small volume protocol and eluted into 50 μL proprietary elution buffer ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Animals were then re-suspended on ice in 50 µl of RIPA buffer with added phosphatase inhibitors (Cell Signalling Technologies #9806) and complete EDTA-free protease inhibitor cocktail (Roche # 04693159001) before sonication ...
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bioRxiv - Microbiology 2021Quote: ... samples were enzymatically analyzed using a D-Lactic Acid/L-Lactic Acid Enzymatic Bioanalysis UV-Test kit and a D-Glucose Enzymatic Bioanalysis UV-Test kit (Roche Diagnostics) with a modified manufacturer’s protocol to reduce total sample size to 300 uL ...
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bioRxiv - Microbiology 2021Quote: ... 0.2 pmoles of gene-specific forward primer and 0.2 pmoles of gene-specific reverse primer was combined with 2 µL of cDNA template into each well of a 96-well plate (LightCycler® 480 Multiwell Plate 96, white, 04729692001, Roche). qPCR amplification was performed using the following cycling conditions ...
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bioRxiv - Plant Biology 2021Quote: ... The obtained library was amplified by emulsion polymerase chain reaction (PCR) using a GS FLK Titanium SV/LV emPCR Kit (Lib-L; Roche Diagnostics), added to a GS FLK Titanium PicoTiterPlate (Roche Diagnostics) ...
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bioRxiv - Plant Biology 2020Quote: ... and 6.8 μL ddH2O were used and the RT-qPCR was performed using a Light Cycler 96 Real-Time PCR Machine (Roche, Germany). Expression levels were calculated using the 2-ΔCT equation (Kilambi et al. ...
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bioRxiv - Biochemistry 2022Quote: ... and subsequent addition of 50 µl lysis buffer supplemented with 1 mM PMSF / ml buffer and 22 µl / ml buffer protease inhibitor cocktail (Complete, Roche Diagnostics). The cell samples were boiled for 3 minutes ...
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bioRxiv - Microbiology 2021Quote: ... Ten nanograms of cDNA were used as a template in a 5- l reaction mixture from a KAPA SYBR FAST qPCR kit (Kapa Biosystems). Primers used are listed in table S3 ...
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bioRxiv - Molecular Biology 2021Quote: ... Library Enrichment was performed in a 30 μl reaction volume containing 20 μl DNA from the previous step and the following final concentrations: 1X KAPA buffer (Roche #KK2502), 0.3 mM each dNTP (Roche #KK1017) ...
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bioRxiv - Microbiology 2022Quote: ... incubated overnight at 32°C and replica-plated on a YES agar plate containing 100 mg/L hygromycin B (Roche, #10843555001). The hygromycin plates were incubated for 1-2 days at 32°C until colonies had formed ...
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bioRxiv - Cell Biology 2022Quote: ... 25 µL of these samples were resolved by SDS-PAGE and analyzed by western blotting with α-HA3 tag antibody (#3F10, Roche) for western blotting ...