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Citations for Roche :
651 - 700 of 6590 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade D UG28R since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2021Quote: ... The imaginal discs were removed and suspended whole in 350μl of sucrose solution (250mM D-sucrose, 10mM Tris-HCl, 1mM. MgCl2, 1x protease inhibitors (Roche)) inside labelled 2ml dounce homogenisers (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... ankle joints were teased apart and shaken at 110 rpm at 37°C for 25 minutes with 2.68mg/mL collagenase D (Roche) in RPMI 1640 (Gibco ...
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bioRxiv - Cell Biology 2022Quote: Tissue leukocytes were extracted from surgically dissociated lung tissue by enzymatic digestion with 2ml enzyme mix (RPMI with 0.625 mg/ml collagenase D (Roche), 0.85 mg/ml collagenase V (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... For experiments in which islets were analyzed using flow cytometry single cell suspensions were generated by digestion with 0.4 Wunsch Units/ml Collagenase D (Roche) and 250 µg/ml DNAse I (Roche ...
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bioRxiv - Immunology 2022Quote: ... Collected tissue was mechanically dissociated by chopping followed by enzymatic digestion for 45 minutes at 37°C in DMEM containing collagenase D (1.5 mg/ml, Roche) and DNase I (60 U/ml ...
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bioRxiv - Cell Biology 2021Quote: ... at 37°C in a 0.4 μm filtered solution of 0.5 mg/mL collagenase D (Roche, Indianapolis, IN, USA), 1 Unit/mL Dispase (Stemcell Technologies ...
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bioRxiv - Physiology 2019Quote: ... Cardiomyocytes were plated on Petri dishes or Ibidi micro-dishes double-coated with Poly-D-lysine/laminin (Roche/Sigma). Cells were maintained under standard conditions (37°C ...
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bioRxiv - Immunology 2021Quote: ... brain and dura mater were chopped and subject to enzymatic digestion in Hanks’ Balanced Salt Solution (HBSS) containing 2.5 mg/mL collagenase D (Roche) and 0.1 mg/mL DNase I (Roche ...
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bioRxiv - Immunology 2021Quote: ... Livers and liver tumours were excised and then minced with scissors before incubation in RPMI/10% (v/v) FBS/2mM EDTA/1.2mg/mL Collagenase D (Roche)/1mg/mL DNase I (Roche ...
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bioRxiv - Immunology 2021Quote: L1/L2 larvae were collected via pipette in 200-300 µl of PBS then added directly to lysing matrix D (1.4-mm ceramic spheres, MagNA Lyser Green Bead tubes, Roche) along with 1 ml of TRIzol LS ...
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bioRxiv - Immunology 2020Quote: ... The organs were chopped mechanically with scalpels and transferred into gentleMACS C tubes containing DMEM supplemented with 1μg/mL Collagenase D (Roche) and 25mg/mL DNase I (Sigma-Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... and lung were further digested in RPMI + 50U/mL DNase I + 1mg/mL hyaluronidase + 1mg/mL collagenase D (Roche) on a shaker at 220rpm for 45 minutes at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... cells were released by incubating gels in a cell culture incubator in a protease solution of 2.5 mg/mL collagenase D (Roche) in PBS until gels were fully degraded ...
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bioRxiv - Immunology 2023Quote: ... Brain and spinal cord were cut into pieces and digested for 30 min at 37 °C with collagenase D (3.75 mg/ml; Roche) and DNase I (1 mg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... dorsal root ganglia of 6 mouse pups between postnatal days 2 and 4 (P2 and P4) were collected in PBS-Glucose (0.3 µM) and incubated for 30 min in 0.125% collagenase D (Roche, Switzerland) and then 10 min in 0.25% trypsin at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... and 20 mM HEPES) supplied with 30 U/ml of human recombinant IL-2 (rIL-2, Roche). Four domains of SUPT16H ...
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bioRxiv - Cell Biology 2021Quote: ... cells were resuspended in complete media plus 10 IU/mL recombinant human IL-2 (rHIL-2, Roche), and seeded in fresh media at 0.5×106 cells/mL every 48 hours ...
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bioRxiv - Neuroscience 2021Quote: ... Vector DNA was transiently transfected into human embryonic kidney (HEK) cells using Fugene-6 (Roche Molecular Biochemicals) transfection reagent ...
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bioRxiv - Immunology 2021Quote: Total RNA was retrotranscribed and cDNA was quantified using the Universal Human Probe Roche library (Roche Diagnostics). Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Cancer Biology 2023Quote: ... and goat anti-human AF647 (Stratech 109-606-088- JIR)) DNA was then stained with DAPI (Roche) and coverslips mounted with Vectashield (Vector Laboratories - Vector H-1000).
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bioRxiv - Immunology 2024Quote: Mice or Human CD4 T cells were lysed using RIPA buffer supplemented with protease inhibitor cocktail (Roche) and phosphatase inhibitors (Pierce) ...
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bioRxiv - Developmental Biology 2021Quote: ... Whole brain lysate was precleared with Protein G agarose (Roche, 11243233001) and the supernatant was immunoprecipitated with CB1R antibody overnight at 4°C ...
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bioRxiv - Genomics 2020Quote: ... The lysate was pre-cleared with protein G-agarose beads (Roche) and incubated with anti-FLAG M2 agarose beads (Sigma #A2220) ...
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bioRxiv - Molecular Biology 2020Quote: ... HA-tagged proteins were detected with mouse (Covance) or rat (Roche) monoclonal anti-V5 antibodies at 1 μg/ml or 12.5 ng/ml respectively ...
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bioRxiv - Molecular Biology 2020Quote: ... The supernatants were incubated and rotated with Protein A beads (Roche) with an anti-Mcm2 antibody (Bethyl ...
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bioRxiv - Plant Biology 2020Quote: ... protein blotting was performed using antibodies against GFP (Roche, Basel, Switzerland) and HA (Roche).
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bioRxiv - Plant Biology 2021Quote: ... HA-tagged proteins were detected with Anti-HA-Peroxidase (Roche #12013819001) at a dilution of 1:5,000 ...
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bioRxiv - Microbiology 2021Quote: ... and pre-cleared with 80 μL of Protein-A agarose (Roche) and 100 μg BSA ...
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bioRxiv - Microbiology 2019Quote: ... The lysates were pre-cleared with protein A-agarose beads (Roche) for 3 h and then incubated with anti-β1-integrin antibodies (MAB2000 from Millipore ...
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bioRxiv - Microbiology 2022Quote: ... To prevent protein degradation a protease inhibitor was added (Complete, Roche). Afterwards ...
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bioRxiv - Immunology 2020Quote: ... urinary protein level using a P800 biochemical analyzer (Roche, Mannheim, Germany), and urinary RBC and white blood cell (WBC ...
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bioRxiv - Genetics 2019Quote: ... The GFP-tagged proteins were probed with anti-GFP antibody (Roche) (1:1,000 ...
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bioRxiv - Molecular Biology 2021Quote: ... Protease inhibitor cocktail and protein phosphatase inhibitor cocktail were from Roche. MS023 (SML1555) ...
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bioRxiv - Immunology 2022Quote: ... containing phosphoSTOP and protein inhibitors cocktail (100 mg/ml, Roche 11836153001) was added to the tube at the ratio of 1 ml buffer per 100 mg tissue ...
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bioRxiv - Plant Biology 2023Quote: ... A volume containing 50 ul of Protein A - agarose beads (Roche) was then added to the extract before overnight incubation ...
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bioRxiv - Neuroscience 2022Quote: ... Protein visualization was performed by chemiluminescence using LumiLight western blotting (Roche) and ImageQuant 800 (GE Healthcare).
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bioRxiv - Molecular Biology 2022Quote: ... protein was extracted with EB2 supplemented with PhosStop (Roche, Indianapolis, IN) and were subjected to IP with 15 μl of settled a-FLAG beads (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... followed by incubation with 20 μl of protein G-beads (Roche) for 1 h on a rotator at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... The target protein was purified using His-Tag Purification Resin (Roche) according to the manufacturer instructions.
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bioRxiv - Developmental Biology 2023Quote: ... Antibody-chromatin complexes were recovered using protein G-agarose beads (Roche). After washing ...
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bioRxiv - Biochemistry 2023Quote: ... FLAG-tagged proteins were detected with a mouse anti-FLAG (Roche) antibody diluted 1:1000 ...
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bioRxiv - Pathology 2023Quote: ... The expressed proteins were detected by using anti-HA-HRP (Roche) at a dilution of 1:5,000 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were visualized using Lumi-Light Western Blotting Substrate (Roche, Switzerland). The signal was detected using the ChemiDOC MP Imaging System (Bio-rad ...
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bioRxiv - Microbiology 2024Quote: ... Monoclonal mouse antibodies were used to detect GFP-tagged proteins (Roche) (dilution 1:2000 ...
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bioRxiv - Cell Biology 2020Quote: Cells were pelleted and lysed with 1.5% n-dodecyl-D-maltoside (DDM) in PBS with cOmplete™ protease inhibitor (Roche) for 15 min on ice ...
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bioRxiv - Molecular Biology 2021Quote: ... Dissected and pulverized TA samples were placed in TRIzol in Lysing Matrix D Tubes (MP Biomedical) and were homogenized in a MagNa Lyser machine (Roche) programmed to 7,000 RPM for a total of three 20-second bursts separated by 10-second cool-downs on ice ...
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bioRxiv - Physiology 2019Quote: ... Serum Vitamin D (S-25-OH-D) levels were measured in samples collected before and after the intervention using a electrochemiluminescence immunoassay (Roche Cobas Vitamin D total assay ...
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bioRxiv - Cell Biology 2019Quote: ... ground yeast powder was solubilized in 4 volumes of homogenization buffer (400 mM trisodium citrate, pH 8, 0.5% n-Dodecyl β-D-maltoside) and protease inhibitor cocktail (Roche). The soluble fraction was incubated with 10 μl magnetic beads (Dynabeads ...
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bioRxiv - Immunology 2022Quote: ... The tonsil and lung were gentleMACS dissociated in 5mL digestion buffer (RPMI + 50U/mL DNase I + 1mg/mL hyaluronidase + 1mg/mL collagenase D (Roche)) and then agitated on a shaker at 220rpm for 45 minutes at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting fat pads were rinsed in PBS and minced in ice-cold digestion buffer (7.5 mg/ml Collagenase D (Roche 1108886001), and 4.8 mg/ml Dispase II (Sigma D4693 ...