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Citations for Roche :
6651 - 6700 of 6963 citations for Rat Antigen peptide transporter 2 TAP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... Final libraries were checked for quality and quantity by TapeStation and qPCR using Kapa’s library quantification kit for Illumina Sequencing platforms (Kapa Biosystems, Wilmington MA). The samples were pooled ...
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bioRxiv - Systems Biology 2019Quote: ... The pooled library DNA was evaluated based on the averaged length and concentration using a Bioanalyzer Agilent High-Sensitivity DNA Kit (Cat# 5067-4626) in the range of 150–3,000 bp and a KAPA library quantification kit (Cat# KK4824, Kapa Biosystems, Wilmington, MA). Finally ...
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bioRxiv - Microbiology 2019Quote: Southern blot was performed using a digoxigenin (DIG) High Prime DNA Labeling and Detection Starter Kit I (Roche Diagnostics GmbH, Mannheim, Germany) as instructed by the manufacturer ...
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bioRxiv - Plant Biology 2020Quote: ... In situ nick-end labeling of nuclear DNA fragmentation was performed for 1 h in the dark at 37°C using the In-Situ cell death detection kit (Roche Applied Science) according to the manufacturer’s manual ...
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bioRxiv - Genomics 2020Quote: ... Whole transcriptome libraries were constructed using the KAPA Stranded mRNA-seq library kit (Roche Sequencing and Life Science, Kapa Biosystems, Wilmington, MA) from 250ng of total input RNA with the Beckman Coulter Biomek FXP automation system (Beckman Coulter ...
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bioRxiv - Plant Biology 2020Quote: ... Blots were hybridized with a digoxygenin (DIG)-labeled ZmPEPC promoter-specific probe synthesized using primers (Fwd 5’-TCCCGAGTTCCTAACCACAG; and Rev 5’-GTGGCTGAGGCTTCTTTTTG) and the PCR DIG Probe Synthesis Kit (Roche Diagnostics, Switzerland). The signals were detected by CDP-Star (Roche Diagnostics ...
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bioRxiv - Synthetic Biology 2020Quote: ... taiwanensis VLB120 was used as the template for the PCR and was isolated using the High Pure PCR Template Preparation Kit (Hoffmann-La-Roche, Basel, Switzerland). Plasmids were constructed by Gibson assembly using the NEBuilder Hifi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Cell Biology 2020Quote: ... Mip1α and housekeeping gene β-actin was evaluated by quantitative RT-PCR using the LightCycler 480 SYBR Green 1 Master kit and LightCycler 480 II (Roche, Indianapolis, IN) and oligos ...
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bioRxiv - Microbiology 2019Quote: ... The concentration of the pooled samples was determined using the Kapa Biosystems library quantification kit for Illumina platforms (Kapa Biosystems, MA, USA). Agilent Bioanalyzer high-sensitivity DNA analysis kit (Agilent CA ...
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bioRxiv - Microbiology 2020Quote: ... and then the pooled libraries were diluted for accurate qPCR quantification using KAPA Library Quantification Kit for Illumina platforms (KAPA Biosystems, MA). The libraries pools were adjusted to a final concentration of 11.5 pM (for V2 kits ...
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bioRxiv - Genetics 2021Quote: ... 500 ng total RNA was used for generating strand-specific RNA-seq libraries using the KAPA Stranded RNA-Seq Kit with RiboErase (Kapa Biosystems, MA). RNA-seq libraries were sequenced on an Illumina NextSeq 500 platform with paired-end 75 bp read length.
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bioRxiv - Immunology 2021Quote: ... 5 μl of the resulting cDNA-containing reverse transcription mixes were then used as templates for 25 μL PCR reactions using the KAPA HiFi PCR kit with GC buffer (Roche Diagnostics, #07958846001) and the following thermal cycling conditions ...
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bioRxiv - Plant Biology 2019Quote: ... and 1 µg RNA was used for cDNA synthesis with oligo(dT)12-18 using Transcriptor first-strand synthesis kit (Roche, Basel, Switzerland). Transcript levels were measured by qRT-PCR using LightCycler 480 SYBR Green I Master (Roche ...
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bioRxiv - Genetics 2020Quote: ... A small portion of the vitelline glands from the posterior part of each fluke was used for DNA extraction using the High Pure DNA Extraction Kit (Roche, Mannheim, Germany) following the manufacturer’s protocols ...
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bioRxiv - Genetics 2021Quote: The bone libraries were prepared in a dedicated low copy DNA laboratory using the KAPA Hyper Prep Kit (Roche Sequencing, Wilmington, MA). Library preparation followed the manufacturer’s recommendations using 15 µM 8-bp dual-indexed adapters ...
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bioRxiv - Genetics 2021Quote: ... The T7 promoter was added to the 5’ and 3’ ends to synthesize the sense and anti-sense probes using the DIG RNA Labeling Kit (Roche, Cat# 11175025910). In situ hybridization was performed as previously described [65,66] with minor modifications ...
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bioRxiv - Physiology 2020Quote: ... Tissues were homogenised in assay buffer provided in the kit with the addition of 5 μL of protease inhibitor cocktail (Roche, Basel Switzerland) and centrifuged at 800 × g for 10 minutes at 4°C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Library preparation was carried out using KAPA Hyper Prep and HiFi HS Library Amplification kits (F. Hoffmann-La Roche AG, Basel, Switzerland) and with iTru i5 and i7 dual-indexing primers (BadDNA ...
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bioRxiv - Cell Biology 2020Quote: ... Southern blotting was performed as instructed in the instruction manual for the DIG-High Prime DNA Labeling and Detection Starter Kit II (Roche Cat. 11585614910).
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bioRxiv - Evolutionary Biology 2021Quote: ... and the product was used for the second round PCR using the primers F and R-T7 for generating antisense probe and primers R and F-T7 for generating sense probe used in ISH assays with the DIG RNA Labeling Kit (Roche, Mannheim, Germany),the primers were shown in table 1 ...
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bioRxiv - Developmental Biology 2021Quote: ... Sequencing libraries were prepared starting from 500 ng for each DNA sample with the KAPA HyperPrep Kit (Hoffman-La Roche, Basel, Switzerland) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Qualitative and quantitative analysis of the NGS library preparation process was conducted using a chip-based electrophoresis and libraries were quantified using a qPCR-based quantification kit (Roche, Cat. # KK4824). Sequencing was performed using Illumina NextSeq500 platform (Illumina Inc) ...
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bioRxiv - Molecular Biology 2021Quote: RNA template (500 ng) was used for one-step RT-PCR amplification protocol (SYBR Green I RNA amplification kit, Roche, Basel, Switzerland). Primers were designed using the LC Primer/Probe design software (Roche ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The primary antibody was either visualized with the multimer-technology based UltraView Universal DAB Detection kit (Ventana® BenchMark® XT; Roche) or a fluorochrome-labeled secondary antibody (Alexa-conjugated ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA probes were synthesized by in vitro transcription under the drive of SP6 promoter with the DIG RNA Labeling Kit (Roche, Mannheim, Germany); Then ...
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bioRxiv - Cancer Biology 2020Quote: ... The libraries were quality controlled on an Agilent 2100 Bioanalyzer with the DNA 7500 assay for size and the concentration was estimated using quantitative PCR with the KAPA Library Quantification Kit Illumina® Platforms (Roche Kapa Biosystems). To obtain sufficient amount of libraries for sequencing it was necessary for the low input libraries (0,1 - 0,2 ug ...
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bioRxiv - Microbiology 2020Quote: ... and prepared for shotgun metagenomic sequencing using the Kapa Hyperprep Plus kit with 3 rounds of PCR amplification (Kapa Biosystems, Wilmington, MA), and sequenced to an average depth of 7 gbp per fraction on the Illumina NovaSeq (Illumina ...
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bioRxiv - Genomics 2021Quote: ... and then either the remaining components of the PowerSoil Pro kit or DNA purification beads from Kapa Biosciences (Roche, Burgess Hill, UK) and either the MPBio SuperFastPrep-2 (MPbio ...
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bioRxiv - Cancer Biology 2021Quote: ... Femurs were then decalcified and paraffin sections (5-μm thickness) were stained with hematoxylin and eosin or reticulin (Reticulum II Staining Kit, Roche, Tucson, AZ) to assess fibrosis ...
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bioRxiv - Microbiology 2020Quote: ... Total nucleic acid was extracted from the lysate using the Roche MagNA Pure Compact Nucleic Acid Isolation Kit I and the MagNA Pure Compact instrument (Roche, Indianapolis, Indiana). Dual-indexed sequencing libraries were prepared from the lysates using Nextera XT DNA Library Preparation Kit (Illumina FC-131-1096) ...
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bioRxiv - Microbiology 2020Quote: Each 300 μL of GEA supernatant sample was extracted using the High Pure PCR Template Preparation Kit (Roche Life Science, Mannheim, Germany), a kit based on silica-membrane spin columns technology ...
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bioRxiv - Immunology 2020Quote: ... CD206 and CD64 was performed by Fast Start DNA Masterplus SYBR Green I kit on the Light Cycler 480 (Roche, Applied Science). Relative RNA expression levels were normalized to the geometric mean of two reference genes RPL37A and ACTB ...
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bioRxiv - Immunology 2021Quote: ... it was added 50 µL of fresh TUNEL reaction mixture composed of 5 µL of enzyme solution mixed with 45 µL of labeling solution (In Situ Cell Death Detection kit, POD, ROCHE, version 15.0) for 1 hour at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... cultured in vitro at 30 °C and at 37 °C in EMJH medium was also extracted using a High Pure RNA Isolation kit (Roche Applied Science) following the manufacturer’s recommendations ...
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bioRxiv - Genetics 2019Quote: ... after which genomic DNA was prepared from the tissue using the KAPA Express Extract DNA Extraction Kit (Kapa Biosystems, cat. nr. KK7103). For both Gulo and Slc2a10 the DNA was PCR-amplified using specific primers detecting the wild type and mutant alleles ...
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bioRxiv - Genetics 2020Quote: ... The membrane was hybridized using digoxigenin-labeled DNA probes (S4 Table) for mPSP based on the DIG-High Prime DNA Labeling and Detection Starter Kit II protocol (Roche, Mannhein, Germany). For western blotting ...
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bioRxiv - Genomics 2019Quote: ... short-read genomic DNA library preparation was performed with the KAPA Library preparation kit in accordance with manufacturer’s protocols (Kapa Biosystems, Wilmington, MA). In the FHBE study ...
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bioRxiv - Microbiology 2022Quote: ... Samples were transferred to a high pure extender assembly from the High Pure Viral Nucleic Acid Large Volume kit (Roche, Mannheim, Germany) and centrifuged for 8 min with 1,500 rpm at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... in situ terminal deoxynucleotidyl transferase deoxyuridin triphosphate nick end labeling (TUNEL) assay was performed using in situ cell death kit from Roche (Basel, Switzerland).
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bioRxiv - Genetics 2022Quote: ... to evaluate library size and adapter dimer presence before the library concentration was determined via quantitative real time PCR using the KAPA library quantification kits (KK4824, Roche, Basel, Switzerland) on the QuantStudio7 or ViiA7 (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... The libraries were quantified by fluorometry on a Qubit instrument (LifeTechnologies, Carlsbad, CA) and by qPCR with a Kapa Library Quant kit (Kapa Biosystems-Roche) prior to sequencing ...
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bioRxiv - Bioengineering 2022Quote: ... Acetate concentration in the supernatant was measured using the Cedex Acetate V2 Bio HT kit on a Cedex HT system (Roche Custom Biotech). OD600 was measured using a Ultrospec 2100 Spectrophotometer (Amersham Biosciences).
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bioRxiv - Developmental Biology 2022Quote: ... Antisense and sense RNA probes of TaDOF5.6 and TaDOF3.4 genes were synthesized using a DIG RNA Labeling Kit (SP6/T7) (Roche, Cat No. 11175025910, USA). The oligonucleotide primers for in situ hybridization are listed in Table S28.
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bioRxiv - Genomics 2022Quote: ... The resulting libraries were purified following the Nextera XT protocol (0.6× ratio) and quantified by qPCR using Kapa Library Quantification kit (Kapa Biosystems, Cat. KK4824). Four plates were pooled together on a NextSeq 550 high-output flow cell or NextSeq 2000 P2-100 flow cell with 26 bp in read 1 and 50 bp in read 2 ...
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bioRxiv - Genomics 2022Quote: ... The amplification of 16S rRNA genes was conducted using the KAPA2G™ Robust HotStart ReadyMix PCR Kit (Kapa Biosystems, Wilmington, MA, USA) in a total volume of 25□μl ...
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bioRxiv - Plant Biology 2022Quote: ... The enriched biotinylated DNA fragments were subjected to library construction on beads using the KAPA HyperPrep Kit for Illumina (Roche, Basel, Switzerland) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: EMSA DNA fragments were labelled with digoxigenin (DIG) following the manufactureŕs instructions (DIG Gel Shift Kit 2nd Generation; Roche Diagnostics, IN, USA) and 30 fmol of labelled DNA was incubated with different amounts of purified HaWRI1_DBD recombinant protein (0-1000 ng ...
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bioRxiv - Plant Biology 2022Quote: ... The libraries were assessed for size and purity using the Agilent 2100 Bioanalyzer and quantitated by qPCR using a KAPA Biosystems Library Quantitation kit (Roche, Pleasanton, CA). Libraries were normalized ...
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bioRxiv - Microbiology 2022Quote: ... Cell equivalents were calculated according to amplification of the β-globin gene (two copies per diploid cell) with commercially available materials (Control Kit DNA; Roche Diagnostics). 2-LTR circles ...
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bioRxiv - Pathology 2022Quote: RNA was reverse-transcribed and amplified using the TaqMan Fast Virus 1-Step Master Mix RT-qPCR kit (LifeTechnologies, Carlsbad, CA) on the LightCycler 480 or LC96 instrument (Roche, Indianapolis, IN), and quantified by interpolation onto a standard curve made up of serial tenfold dilutions of in vitro transcribed RNA ...