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Citations for Roche :
601 - 650 of 705 citations for 4 4' Di N acetylamino diphenylsulfone d8 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... and incubated overnight at 4°C with anti-Digoxigenin-AP-conjugated antibody (dilution range: 1:1000 to 1:5000, Roche, catalog no. 11093274910) diluted in the same buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... Strand-specific RNA libraries were prepared for sequencing (3 - 4 biological replicates/treatment) using a KAPA Stranded mRNA-Seq Kit (Kapa Biosystems, MA, USA). Poly-A mRNAs were purified from 100 ng of total RNA using poly-T-oligo-magnetic beads (Kapa Biosystems ...
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bioRxiv - Genetics 2021Quote: ... with 3x protease inhibitors (cOmplete Protease Inhibitor Cocktail EDTA-free - 1 mM phenylmethylsulfonyl fluoride, 4 mM benzamidine, 2 μg/ml leupeptin, and 1 μg/ml pepstatin, Roche Diagnostics, cat. number 1187358001) and 3x phosphatase inhibitors (Millipore Inhibitor Cocktail Set ...
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bioRxiv - Systems Biology 2021Quote: The digested lysates were mixed 1:1 with 2x IP buffer (200 mM Tris, pH 8.0; 600 mM NaCl; 4% Triton X-100; 2x Roche Complete EDTA-free protease inhibitors), and then kept on ice for no more than a few hours prior to antibody addition ...
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bioRxiv - Immunology 2020Quote: ... Lyzed cells were centrifuged at 20,000 x g for 30 min at 4°C and the supernatant was mixed with protease inhibitor (cOmplete ULTRA Tablets, Roche, Sigma-Aldrich, Cat: 05892953001). Protein concentration was determined using the Qubit Protein Assay Kit and Fluorometer (Invitrogen) ...
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bioRxiv - Bioengineering 2021Quote: ... expanded leaves of 7-week old hybrid aspen plants were sampled with a biopsy punch (4 mm diameter) and loaded into wells of 96-well plate (Roche LightCycler® 480 System) containing 100 μL of water (one sample per well) ...
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bioRxiv - Physiology 2022Quote: ... This was followed by a perfusion at 4 mL/min for 40 min with the same solution containing 1 mg/mL of collagenase A (Roche Diagnostics GmbH, Mannheim, Germany) plus 300 µM ethylene glycol tetraacetic acid (EGTA ...
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bioRxiv - Developmental Biology 2021Quote: ... Probes were hybridized at 56°C overnight and subsequently detected by anti-DIG at 4°C overnight (1:1000; Roche 11-207-733-910) with secondary tyramide signal amplification for 2h at room temperature in the dark (AKOYA Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... After three washes with PBS containing 0.5% Tween-20 samples were incubated with fluorescent labelled secondary antibodies containing 4′,6-diamidino-2-phenylindole (DAPI; Roche Cat# 10236276001, 1.0 µg/ml) for 30 min ...
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bioRxiv - Zoology 2023Quote: ... Paired-end cDNA libraries were constructed from 4 μg of total RNA using a KAPA Stranded mRNA-Seq Kit (Kapa Biosystems, Inc., Wilmington, USA) according to the manufacturer’s instructions ...
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bioRxiv - Zoology 2024Quote: ... Samples were pre-incubated for 2 h in blocking solution (25% deactivated goat serum in PBT) and incubated overnight at 4°C in blocking solution with anti-DIG antibody conjugated to alkaline phosphatase (Roche Diagnostics, Germany; 1:2000). After several washes in PBT ...
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bioRxiv - Biophysics 2022Quote: ... synthesized by IDT) with 66 mM digoxigenin N-hydroxysuccinimide ester (Roche) in 50 mM HEPES-NaOH ...
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bioRxiv - Biochemistry 2020Quote: ... N-glycans were released with 2.5 U PNGase F (Roche, Australia) for 16 h at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1% N-Lauroylsarcosine supplemented with 1mM PMSF and protease inhibitors (Roche)) at the concentration of 4×107 cells/ml ...
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bioRxiv - Biophysics 2024Quote: ... Hen egg white lysozyme was purchased from Roche (Cat. N° 10837059001) or from Seikagaku Corp ...
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bioRxiv - Immunology 2020Quote: ... and a 30-gauge needle was used to inflate each pancreas through the common bile duct with 4 mL of media supplemented with 0.8 mg/mL of collagenase P (Roche, cat. number 11-249-002-001) and filtered at 0.22 μm prior to injection ...
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bioRxiv - Biochemistry 2021Quote: ... 10 mM N-ethylmaleimide) with 1x Complete EDTA-free protease inhibitors (Roche) and 6 μl benzonase (Millipore ...
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bioRxiv - Cell Biology 2021Quote: ... DUB inhibitor (10 mM N-ethylmaleimide) and phosphatase inhibitor (PhosphoStop, Roche, USA) using a POLYTRON hand homogenizer(19) ...
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bioRxiv - Genetics 2020Quote: ... acid-extracted histones were digested with Asp-N and Arg-C (Roche) and the resulting digests were analyzed by chromatography on an Ultimate 3000 nanoLC (Dionex ...
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bioRxiv - Microbiology 2024Quote: ... and 0.5% n-octyl-β-D-glucopyranoside) supplemented with protease inhibitors (Roche), and ultrasonicated for protein extraction ...
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bioRxiv - Microbiology 2024Quote: ... the skin was disinfected as above and the dermis and epidermis were separated by overnight dispase II treatment (Roche, #4942078001, 10 mg/mL in HBSS, 4°C). Fibroblasts were obtained from minced dermal tissue by overnight collagenase digestion (Worthington ...
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bioRxiv - Cancer Biology 2021Quote: ... Peptide N-glycosidase F (PNGase F, lyophilized, glycerol-free) was purchased from Roche Diagnostics (Mannheim ...
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bioRxiv - Physiology 2022Quote: ... containing protease inhibitors (protease inhibitor cocktails tablets, catalog n° 48047900, Roche, Basel, Switzerland). After high-speed shaking in TissueLyserII (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... N-glycans were released by incubation with 1.0 mU PNGase A (Roche Diagnostics) in 60 μL 0.5 M citrate/phosphate buffer at pH 4.0 for 16 h and purified using a Carbograph Ultra Clean column (Alltech ...
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bioRxiv - Developmental Biology 2022Quote: The In Situ Cell Death Detection Kit Fluorescein (Roche, N°11684-795-910) was used for detecting dying cells in intact Hv_Basel ...
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bioRxiv - Biophysics 2023Quote: ... solubilized with 1 % final concentration n-Dodecyl β-D-maltoside (Roche, Mannheim, Germany) and incubated for 5 min at 25 °C ...
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bioRxiv - Immunology 2023Quote: ... 20 mM N-ethylmaleimide) supplemented with cOmplete Mini EDTA-free protease inhibitors (Roche), and centrifuged at 20,000 x g for 30 min ...
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bioRxiv - Genomics 2020Quote: ... or the KAPA Library Quantification Kit (scRNA-seq libraries only; Roche, Cat. N: 07960298001). Size distribution of the obtained libraries was assessed using Agilent 2100 Bioanalyzer ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.5% N-Lauroylsarcosine) and resuspended in 100 μl complete LB3 (LB3 containing 1x Roche cOmplete Mini ...
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bioRxiv - Microbiology 2020Quote: ... and precB5R-N+GPC by using X-tremeGENE 9 (Roche Diagnostics K.K., Tokyo, Japan). Cells transfected with each plasmid were then infected with m8 at a multiplicity of infection (MOI ...
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bioRxiv - Microbiology 2023Quote: ... 2 % N-lauroylsarcosine (w/v)) supplemented with Protease Inhibitors Cocktail (cOmplete ULTRA Tablets, Roche), DNaseI (1 µg/ml ...
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bioRxiv - Physiology 2024Quote: ... Blocking was performed using a blocking solution (western blocking reagent Roche catalog N°11921673001) containing 0.15% Triton X-100 and 1% BSA in 300 mM Glycine for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.8% CHAPS with a cOmplete™ protease inhibitor cocktail tablet added (Roche, cat n°11697498001) for 10 min on ice and centrifuged for 15 min at 16,000 x g at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... with a primary antibody for α-syn (Novocastra N-ASYN, clone KM51, Roche; 1:25) and a secondary antibody ...
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bioRxiv - Microbiology 2022Quote: ... qPCR was performed using with FastStart Universal SYBR Green Master Mix (Roche, Cat. N° 4385610). Primers (Supplementary Table 2 ...
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bioRxiv - Developmental Biology 2021Quote: ... and incubated O/N in the same solution containing 1:2,500 anti-digoxigenin antibody (Roche). The next day the embryos were washed in TBST ...
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bioRxiv - Cell Biology 2022Quote: ... Remaining lysate was rotated O/N with 25 µl of Protein-A-agarose beads (Roche) and 2 µg of mouse anti-HA antibody (Supplementary Table S1) ...
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bioRxiv - Genomics 2024Quote: ... 0.5% N-Lauroylsarcosine) and resuspended in 100 μl of complete LB3 (LB3 containing 1 × Roche cOmplete Mini Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Molecular Biology 2020Quote: ... 1% n-dodecyl β-D-maltoside [DDM] and 1X cOmplete™ EDTA-free protease inhibitor (Roche)) and incubated at 4°C for 30 mins ...
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bioRxiv - Genomics 2021Quote: ... Second-stage digestion of SUMOylated peptides was performed with 1 μg of Endoproteinase Asp-N (Roche). Digestion was performed overnight ...
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bioRxiv - Plant Biology 2023Quote: ... supplemented with 1% n-dodecyl β-D-maltoside (DDM) and protease inhibitor cocktail (4693116001, Roche, USA). Homogenization was achieved by gently mixing on ice for 20 min ...
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bioRxiv - Microbiology 2024Quote: ... The recognized protein bands were visualized by 3,3 N-Diaminobenzidine tertrahydrochloride (DAB) solution staining (Roche, Germany).
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bioRxiv - Molecular Biology 2024Quote: ... before being spotted in 2.5 mcl drops onto a dry nylon membrane (Hybond-N+, Roche 11417240001). Biotinylated oligo-dT (Promega Z5261 ...
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bioRxiv - Cell Biology 2024Quote: ... 10ng of total RNA were processed using the KAPA mRNA HyperPrep Kit (Roche p/n KK8580) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... using the LightCycler FastStart DNA Master plus SYBR Green I kit (catalog n° 03515869001, Roche, Basel, Switzerland). 40ng of reverse transcribed RNA was used as template for each reaction ...
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bioRxiv - Genomics 2020Quote: ... Transcripts were quantified by using LightCycler® 480 SYBR Green I Master Mix (Roche, Cat. N: 04707516001) with the appropriate primers (see key resource table ...
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bioRxiv - Physiology 2020Quote: ... the boiled samples were supplemented with 0.8% NP-40 and 1 Unit of N-glycosydase F (Roche) in a final volume 50ul and incubated at 37°C overnight ...
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bioRxiv - Biochemistry 2021Quote: ... 0.5% n-dodecyl β-D-maltopyranoside (DDM) and 1 mM EDTA supplemented with protease inhibitor tablets (Roche) and 1 mM PMSF for 1 hour at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... the polyclonal murine anti N-terminal MSPDBL2 serum and a rat monoclonal α-HA antibody (Roche 3F10) were used ...
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bioRxiv - Cancer Biology 2024Quote: ... N-glycopeptides were enzymatically deglycosylated and eluted from the beads using 5 U of PNGase F (Roche) in 200 µl of 100 mM NH4HCO3 at 37 °C overnight ...