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Citations for Roche :
6251 - 6300 of 6945 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade D UG28R since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Cells were incubated with 8-keto-NeuAc derivatives for 24 h and proliferation measured using WST-1 (Roche Applied Science) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μg of total RNA from each sample was converted into cDNA with First Strand cDNA Synthesis Kit (Roche, #04897030001). Primers used in the RT-qPCR are listed in Supplementary Table S1 ...
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bioRxiv - Molecular Biology 2023Quote: ... upon which cells were washed with 2x ice-cold PBS and lysed in RIPA buffer with 1× EDTA-free protease inhibitor cocktail (Roche). Lysates were then separated on a 17.5% acrylamide gel for 6h at 150V and transferred onto a 0.22µM nitrocellulose membrane using semi-dry transfer ...
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bioRxiv - Molecular Biology 2023Quote: ... and each was lysed by trituration in 1 mL ice-cold PBS with 0.25% Triton-X containing cOmplete™ mini EDTA-free protease inhibitor cocktail tablet (Roche) at a concentration of 10% w/vol followed by a 15-minute incubation on ice ...
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bioRxiv - Neuroscience 2022Quote: ... The cells were washed in chilled 1X PBS and subjected to dounce homogenization in lysis buffer (5mM HEPES, 85mM KCl, 1% IGEPAL CA-630 in 1X PBS, 1X Roche Complete protease inhibitors ...
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bioRxiv - Neuroscience 2022Quote: ... were transfected into HEK293 cells at a ratio of 3:3:1 (hGluN1/hGluN2A/EGFP) using X-tremegene HP (Roche) at a 1:100 ratio with optiMEM ...
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bioRxiv - Molecular Biology 2022Quote: ... cell pellets were resuspended in 1 mL Equilibration Buffer B containing protease-inhibitor mix (Complete EDTA-free; 11 873 580 001, Roche) and 0.2 mg/mL lysozyme ...
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bioRxiv - Neuroscience 2022Quote: ... Cell pellets were homogenized in lysis buffer (7 M urea, 2 M thiourea, 1% CHAPS, 70 mM DTT, and Roche EDTA-free complete protease inhibitor cocktail ...
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bioRxiv - Plant Biology 2022Quote: ... with Horse Radish Peroxidase (HRP)-conjugated antibody (HRP-conjugated anti-HA antibody, 1/1000 [50 mg/mL], cat. no. 12013819001, Roche; HRP-conjugated anti-FLAG antibody ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were diluted 1:3 in ddH2O on ice in a 45 μL total volume before analysis in the Cobas c111 Analyzer (Roche). The Cobas c111 Analyzer is a platform for clinical chemistry testing of human samples but it can also be used to analyze mouse samples ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 mM EDTA, 0.1% NP-40, 1 mM DTT, 10 mM NaF, and EDTA-free protease inhibitor cocktail, Roche, Germany), and centrifugation at 20,000 × g for 3 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Sonicated samples were centrifuged and the supernatant (soluble fraction) was incubated for 1 h at 4°C with beads crosslinked to anti-GFP antibody (11814460001; Roche) or to M2 anti-FLAG antibody (F1804 ...
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bioRxiv - Cancer Biology 2022Quote: A total of 20-50 mg of snap-frozen melanoma tissues were pulverized by Geno/Grinder for 2 min at 1500 rpm and then fixed with 1% methanol-free formaldehyde plus protease inhibitor cocktails (Roche) for 10 min at room temperature and quenched by 125 μM glycine for 5 min at room temperature ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cells were resuspended in lysis buffer (100 mM Pi pH 7, 100 mM NaCl, 1 mM DTT, C0mplete protease inhibitor tablet, Roche) and lysed by sonication for 8 min (Vibracell ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was sonicated in homogenization buffer (1% SDS, 50 mM Tris pH 7.4, 2 mM EDTA, protease and phosphatase inhibitors (Roche 11873580001; Roche 04906837001), heated at 95° C for 5 minutes ...
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bioRxiv - Plant Biology 2024Quote: ... First-strand complementary DNA synthesis was performed using 1 µg of total RNA using an anchored oligo (dT) and Transcriptor Reverse Transcriptase (Roche) following the manufacturers’ protocol ...
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bioRxiv - Neuroscience 2024Quote: ... co-cultures were treated every 48 h from the start of co-culture with either 1 µM LGMN inhibitor (Roche) or a DMSO control for three weeks.
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bioRxiv - Systems Biology 2024Quote: ... The lungs were perfused through the pulmonary artery and inflated via the airway with DMEM containing 1 mg/ml Collagenase/Dispase (Roche), 3 U/ml Elastase (Worthington) ...
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bioRxiv - Molecular Biology 2024Quote: ... and twice with TE wash buffer (10 mM Tris-HCl pH 8, 1 mM EDTA, 1x Roche protease inhibitor mixture).
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bioRxiv - Cell Biology 2023Quote: ... cells were washed twice with PBS and lysed using a lysis buffer (50mM Tris, pH7.4; 150mM NaCl; 1% NP-40) supplemented with protease (Roche, #11836153001) and phosphatase inhibitors (Roche ...
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bioRxiv - Microbiology 2024Quote: ... and 1 μL of the reverse transcrip-tion mix from the Titan One Tube RT-PCR System kit (Roche, Switzerland) was added ...
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bioRxiv - Cell Biology 2024Quote: ... All lysis buffers were supplemented with phosphatase inhibitors NaF (10 mM) and Na3VO4 (1 mM) along with 1X protease inhibitor cocktail (ROCHE cOmplete Protease Inhibitor Cocktail Cat # 1169498001 ...
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bioRxiv - Cancer Biology 2024Quote: Cell lysates were prepared using a 1% triton X-100 containing lysis buffer (CST) supplemented with protease inhibitor cocktail (Roche) on ice for 15 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Prehybridization for 60 min and overnight hybridization were carried out with a DIG-labeled probe (1 µg) at 65 °C with DIG Easy Hyb Granules (Roche). A chemiluminescent assay was used to visualize the probe on the membrane ...
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bioRxiv - Immunology 2023Quote: ... minced with scissors and razor blade in the presence of 1 ml of digest media (2 mg/ml collagenase IV (Roche), 1 mg/ml hyaluronidase (Worthington) ...
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bioRxiv - Pathology 2024Quote: ... cDNA product was 1 to 40 diluted and real-time PCR was performed using FastStart SYBR Green Master Mix (Roche) on QuantStudio 5 (ThermoFisher ...
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bioRxiv - Microbiology 2024Quote: ... The gDNA levels corresponding to the viral genes and the housekeeping wasp gene (elongation factor (ELF-1)) were determined using the LightCycler 480 System (Roche). The cycling conditions involved heating at 95°C for 10 min ...
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bioRxiv - Microbiology 2024Quote: ... and lysed by incubation with nondenaturing lysis buffer (300 mM NaCl, 100 mM Tris-HCl [pH 7.4], 2% Triton X-100, and 1×EDTA-free protease inhibitor cocktail [Roche Complete]) for 30 min on ice ...
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bioRxiv - Molecular Biology 2024Quote: ... 15 µl of IPed proteins and 10 µl of WCE were submitted to SDS-PAGE and analyzed by immunoblotting using anti-HA antibody (1:250, ref.12013819001, Roche), anti-Flag antibody (1:500 ...
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bioRxiv - Microbiology 2024Quote: ... The bacterial pellets were then resuspended in 20 ml of lysis buffer pH 8.0 (50 mM NaH2PO4, 300 mM NaCl, 5 mM Imidazole) containing 1× cOmplete EDTA-free protease inhibitor cocktail (Roche, Germany) and 1 mg/ml lysozyme ...
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bioRxiv - Neuroscience 2024Quote: ... colorimetric reaction was performed for 1-2 days at room temperature in a solution containing NBT (nitro-blue tetrazolium chloride, Roche) and BCIP (5-bromo-4-chloro-30-indoly phosphate p-toluidine salt ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were harvested and stored at -80°C in ICD lysis buffer (300 mM NaCl, 50 mM Tris/HCl, 1 mM EDTA, 1x Protease Inhibitor (Roche), pH 7.4) ...
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bioRxiv - Cell Biology 2024Quote: ... The membranes were blocked in 5% non-fat milk in TBST for 1 h and immunoblotted with an antibody against HA directly conjugated to HRP (anti-HA-HRP; Roche) for 1 h at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Different dilutions of the enzyme preparation were assayed for 1 hour and analyzed with a luciferin-luciferase coupled reaction (76) using the CLSII-Bioluminiscent ATP Assay Kit (Roche).
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bioRxiv - Molecular Biology 2024Quote: ... whole cell lysate samples were prepared by resuspension and trituration of cell pellets in a lysis buffer (20 µM Tris-HCl pH 7.4, 1 µg/mL DNaseI, 1X cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche)) ...
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bioRxiv - Developmental Biology 2024Quote: ... Phosphorylated and annealed oligos were diluted 1:10 and a Golden Gate reaction was setup with 1x rapid ligase (Roche), 10 units of BbsI enzyme ...
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bioRxiv - Microbiology 2023Quote: Cells were washed in PBS and harvested in lysis buffer (1% NP-40, 2mM EDTA, 10% glycerol, 150mM NaCl, 50mM Tris-HCl, protease inhibitor (Roche complete mini EDTA-free protease inhibitor) ...
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bioRxiv - Plant Biology 2023Quote: ... Detection of alkaline phosphatase was carried out for ∼1 h using NBT/BCIP ready-to-use stock solution (La Roche) diluted in 1× washing buffer.
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bioRxiv - Neuroscience 2023Quote: ... the supernatant replaced by 1ml PBS-Glucose supplemented with 50 µl Collagenase/Dispase (Worthington CLS-1 345U/mg; Dispase II Roche 1.2U/mg ...
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bioRxiv - Plant Biology 2024Quote: ... The transformants were screened on MS plants with 1% sucrose and 0.8% agar containing 40mg/L hygromycin (Roche, no. 10843555001). For each construct ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA (1 μg) was reverse transcribed to cDNA using the Transcriptor First Strand cDNA Synthesis Kit (catalog no. 04379012001, Roche), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mM EDTA and 1% Triton X-100) containing 2 mM Na3VO4 (Sodium Orthovanadate) and 1 mM pmsf (phenylmethylsulfonyl fluoride) and 1x protease inhibitor cocktail tablet (Roche). Whole cell lysates were centrifuged at 12,000 rpm at 4 0C for 10 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were permeabilised by 10 minutes incubation with 0.1% Triton X-100 on ice and incubated with blocking buffer (10% FBS in 1X PBS) for 90 minutes and then probed with the primary antibody (anti-HA rat 1:100 (Roche) for 2 hours on rotor and after three washing with 1x PBS cells were probed with anti-rat Alex Flour-488 (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... membranes were incubated with blocking solution (5% skimmed milk in 1X PBS) followed by overnight incubation with respective primary antibodies (anti HA rat 1:1000 (Roche), anti BiP rabbit 1:5000 in cold room and 1 hr incubation with respective secondary antibodies (1:10000 ...
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bioRxiv - Cell Biology 2023Quote: Frozen liver tissues that were crushed using a Multi-beads Shocker (Yasui Kikai, Japan) or cultured cells were dissolved in RIPA buffer containing 1× cOmplete proteinase Inhibitor Cocktail (Roche). Protein concentration in the lysates was determined using a BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were then resuspended in cold lysis buffer (10 mM Tris, pH 7.5, 10 mM NaCl, 5 mM MgCl2, 1 mM EGTA, Protease Inhibitor (Roche, 04693159001)) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The final pellet was resuspended in 750 uL nuclear lysis buffer (10 mM EDTA, 1% SDS, 50 mM Tris-HCl at pH 8, 1x cOmplete protease inhibitor cocktails (Roche)) ...
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bioRxiv - Developmental Biology 2024Quote: ... blocked in MAB-Buffer2 (20% sheep serum, MAB-Buffer1) for 1h and incubated with anti-DIG-AP antibody (1:4000, Roche) in MAB-Buffer2 overnight at 4 °C.
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bioRxiv - Genomics 2023Quote: ... and collected in lysis buffer (1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS and protease inhibitor [#11873580001, Roche] in PBS). Chromatin was sonicated with a Covaris E220 sonicator (140PIP ...
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bioRxiv - Immunology 2024Quote: ... Lungs were resected and minced with scissors before incubation in a collagenase buffer containing 1 mg/mL Collagenase-A (Roche), 2000U DNaseI (Sigma) ...