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Citations for Roche :
6251 - 6300 of 8748 citations for 7 Chloro 1 3 dihydro 5 phenyl 2H 1 4 benzodiazepin 2 thione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... cells were washed in ice-cold PBS and lysed in lysis buffer (100 mM TrisHCl pH 7.5, 150 mM NaCl, 1% Triton X-100, protease inhibitors (Roche)) for 30 minutes on ice ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were lysed in lysis buffer (20 mM HEPES pH 7.5, 500 mM NaCl, 10% glycerol, 1 mM DTT and complete EDTA-free protease inhibitors cocktail [Roche]) supplemented with 0.02% NP40 and 1 mM PMSF ...
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bioRxiv - Microbiology 2019Quote: ... Pellets were lysed in 5 pellet volumes of RIPA buffer (25 mM Tris, 150 mM NaCl, 1 % Triton X-100, 0.5 % Sodium deoxycholate, 0.1 % SDS, pH 7.5, 1x Roche Protease Inhibitors) supplemented with 50 units BaseMuncher (Expedeon ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were then vigorously resuspended in 1ml of fresh ice-cold lysis buffer (50mM Tris pH 8, 150mM NaCl, 0.5% NP-40, 1 tablet of complete protease inhibitor (04693159001, Roche)) and transferred to 9 ml of pre-chilled lysis buffer and incubated for 20 min on ice ...
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bioRxiv - Molecular Biology 2019Quote: ... Every 10 million cells were lysed by 300μL 1% SDS include freshly made Protease Inhibitor (PI) cocktail (Roche #4693132001), sonicated with Diagenode Bioruptor Pico and diluted with 2.7mL PBA (1x PBS + 0.5% BSA ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.15 μM each of PaaDlp-2_R1 and R2 in 1 × KAPA HiFi HotStart ReadyMix (KAPA Biosystems, Wilmington, WA, USA) and a 2-μL sample of eDNA ...
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bioRxiv - Microbiology 2019Quote: ... The solution was then supplemented with 20 mM MgSO4 and treated with 50 μg mL-1 RNase A (Roche) and 10 μg mL-1 DNase (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... the proteins were blotted onto a nitrocellulose membrane and probed with anti-GFP (1:1000; Sigma-Aldrich and Roche), anti-dgt6 (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... before lysing in lysis buffer ((1% Triton X100, 50 mM HEPES, 150 mM KCl, 1 mM MgCl2, pH 7.4, with 1x EDTA-free protease inhibitors, Roche) at 4 °C for 2 hrs ...
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LIR-dependent LMX1A/LMX1B autophagy crosstalk shapes human midbrain dopaminergic neuronal resiliencebioRxiv - Cell Biology 2019Quote: ... The samples were incubated on ice for 10min and then lysates were diluted 1:2 with CNBr IP wash buffer (50 mM Tris–HCl pH 7.5, 150mM NaCl, 1mM EDTA, 1/50 protease inhibitor tablets [Roche]). The homogenates were incubated on ice for 15min and were cleared by centrifugation at 12,000g for 15min at 4°C ...
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bioRxiv - Genetics 2021Quote: Neuronal cultures were lysed in 20 µl of NP40/SDS buffer (1% Nonidet P40/0.01% SDS lysis buffer and protease inhibitors (Roche) per well ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated for one hour in donkey anti-mouse FITC antibody (1:200) in 0.3% Triton-X100 supplemented with 1X blocking buffer (Roche). After washing with PBS for 5 min ...
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bioRxiv - Immunology 2021Quote: ... Pellets of cross-linked cells were resuspended in shearing buffer (10 mM Tris-HCl pH 8, 0.1% SDS, 1 mM EDTA) supplemented with Complete Protease Inhibitor Cocktail (Roche), and chromatin was sheared to an average fragment size of 200-250 bp ...
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bioRxiv - Genetics 2020Quote: ... Cells pellets were combined and lysed in a denaturing urea buffer (8 M urea, 0.1 M ammonium bicarbonate, pH 8, 150 mM NaCl, 1 Roche mini protease inhibitor tablet without EDTA/10 ml ...
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bioRxiv - Cell Biology 2021Quote: ... A total of ∼ 8×107 cells were lysed in 2 mL lysis buffer (20 mM Tris HCl pH 7.4, 150 mM NaCl, 1% Triton-X 100 supplemented with protease inhibitor Complete, Roche). Lysates were rotated at 4°C for 20 minutes and subsequently centrifuged at maximum speed for 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... coated with H-2Kb-OVA and ICAM-1 at a 1:1 ratio for various times at 37 ºC and immediately lysed in 2× cold lysis buffer containing phosphatase inhibitors (1 mM NaF and 0.1 mM Na3VO4) and protease inhibitors (cOmplete mini cocktail, EDTA-free, Roche). Activation of PI3K and MAP kinase signaling was assessed by immunoblot for pAkt (Phospho-Akt (Ser473 ...
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bioRxiv - Immunology 2021Quote: Single-cells were sorted into PCR tubes containing 1μl of cell lysis solution (1:10 Cell Lysis buffer(Roche), 10U/μl Rnasin plus Ribonuclease inhibitor (Promega ...
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bioRxiv - Cancer Biology 2021Quote: GSDMB2-HA expression was analyzed by immunohistochemistry in 5µm-thick tissue sections using rat anti-HA (1:200; 3F10, ROCHE) or mouse monoclonal anti-GSDMB (1:10 ...
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bioRxiv - Biochemistry 2020Quote: ... The medium was discarded and the cells were resuspended in 200 µL of ice-cold 50 mM HEPES buffer pH 7.4 containing 1 x protease inhibitor cocktail (Roche) and 1 mM phenylmethylsulfonyl fluoride ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were harvested 4 hours after induction at 37°C and lysed by sonication in 1x PBS pH 7.5 with 1 tablet protease inhibitor cocktail (Roche). After cell lysis and centrifugation at 30,000g ...
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bioRxiv - Neuroscience 2021Quote: ... 1x PBS; Invitrogen, 0.5% RNase free BSA;Serva, 1:200 RNaseIN plus inhibitor; Promega, 1x EDTA-free protease inhibitor; Roche) and filtered through 40 μm filter (BD falcon ...
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bioRxiv - Neuroscience 2020Quote: ... The ipsi- and contralateral cortices were immediately transferred to lysis buffer (50 mM Tris, pH 7.6, 150 mM NaCl, 1% Triton X-100, CompleteMini Protease Inhibitor Mixture, Roche). The cortices were homogenized and incubated on ice for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... cells were washed with ice-cold 1x PBS added with 1.5 mM Na3VO4 and 50mM NaF before being lysed in lysis buffer (1X PBS, 1% Tx-100, 1.5 mM Na3VO4, 50mM NaF, PhosSTOP (Roche) and cOmplete protease inhibitors (Roche)) ...
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bioRxiv - Biochemistry 2021Quote: ... Mitochondria were resuspended in SEM buffer (250 mM sucrose, 1 mM EDTA, 10 mM MOPS-KOH, pH 7.2, containing 1X protease inhibitor cocktail from Roche).
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bioRxiv - Cell Biology 2021Quote: ... in lysis buffer (150 mM NaCl, 1% NP-40, 50mM Tris pH 8.0, 1x Roche protease inhibitor tablet (11697498001)) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were washed with ice-cold 1x PBS added with 1.5 mM Na3VO4 and 50mM NaF before being lysed in lysis buffer (1X PBS, 1% Tx-100, 1.5 mM Na3VO4, 50mM NaF, PhosSTOP (Roche) and cOmplete protease inhibitors (Roche)) ...
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bioRxiv - Microbiology 2020Quote: Adherent macrophages in 6-well plates were washed with PBS containing 1 mM sodium orthovanadate and 10 mM 1,10-phenanthroline (Roche) on ice prior to lysis ...
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bioRxiv - Plant Biology 2020Quote: ... and homogenized in 100 μl ice-cold homogenization buffer (1% [v/v] SDS, 25 mM Tris/HCl, pH 7.5, 150 mM NaCl, 10 mM DTT and a Roche Complete protease inhibitor 1 tablet/10 ml) ...
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bioRxiv - Plant Biology 2021Quote: ... and 250 µl extraction buffer (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 1 % Nonidet P40, proteinase inhibitor cocktail (Roche)) per 200 mg tissue powder was added ...
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bioRxiv - Genomics 2021Quote: ... 1 mL of lung dissociation medium (100 µg/mL of DNAse I, Roche; 1.25 mg/mL of Pronase, Roche; 9.2 µg/mL of Elastase ...
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bioRxiv - Genomics 2020Quote: ... Nuclei were washed and re-suspended in nuclei lysis buffer (50mM Tris-HCl pH7.5, 10mM EDTA, 1% SDS, 1x Complete Proteinase Inhibitor (#11697498001, Roche)) ...
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bioRxiv - Neuroscience 2021Quote: Cells were lysed in ice cold 1% Triton lysis buffer (20mM Tris-HCl pH 8.0, 10mM EDTA, 1% Triton X-100 and 1x protease inhibitor cocktail (5892970001, Roche)) and protein concentration was measured using Bio-Rad BSA protein assay (5000116 ...
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bioRxiv - Physiology 2021Quote: ... Blood glucose was monitored before and after 1-hour of glucose administration using Accu-Chek glucometer (Roche Diagnostics, India). Fasting and post glucose load PBMCs were isolated from freshly obtained whole blood from the donor mice using density gradient separation with Histopaque-1083 ...
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bioRxiv - Microbiology 2021Quote: ... pH 8 containing 150 mM NaCl, 1% Nonidet-P40, 0.1% SDS, 10 mM NaF, and EDTA-free protease inhibitor cocktail [Roche]). Cellular debris and bacteria were removed by centrifugation and collected as the bacterial fraction ...
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bioRxiv - Developmental Biology 2021Quote: ... 20% heat-inactivated goat serum and then incubated overnight with anti-DIG-AP antibody (Roche – 11093274910; 1:2,500 dilution) at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... The cell pellets were resuspended in 1 ml ZAP buffer containing a protease inhibitor (50 mM Tris-HCl pH 7.5, 200 mM NaCl, 1 complete tablet per 50 ml buffer (Roche)) and cells disrupted three times for 45 sec at 6 m/s using an MP Biomedicals™ Fastprep-24 machine ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were incubated with an anti-DIG antibody conjugated with horse radish peroxidase (HRP) (1/500, Roche Diagnostics) for 6 hours at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: Whole-cell lysates from cell lines were prepared using NP40 lysis buffer [20 mM Tris-Cl pH 7.8‚137 mM NaCl and 1% NP-40 supplemented with 1X protease inhibitor cocktail (Roche)].The tissue lysate was prepared from mouse ...
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bioRxiv - Cell Biology 2021Quote: ... pH 7.9, 10 mM KCl, 0.1 mM EDTA, 0.3 % NP-40 and 1 x protease and phosphatase inhibitors, Roche, USA) by passing through a 23G needle and syringe 30 times and then left to stand on ice for 5 min ...
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bioRxiv - Cell Biology 2021Quote: ... 25 mM NAF and 10 μM ZnCl2 which was supplemented with 1 x cOmplete protease inhibitor cocktail (Roche, 11836170001), 1 mM PMSF (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... After addition of the extraction solution containing 1% acetic acid and Complete Mini protease inhibitor cocktail (Roche, Basel, Switzerland) in 1:2 w/v proportion ...
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bioRxiv - Biophysics 2020Quote: ... a 500 mL cell pellet of Sf9 cells was thawed in 50 mL lysis buffer (25 mM HEPES pH 7.4, 1 M KCl, 10% glycerol, Roche protease inhibitor ...
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bioRxiv - Cancer Biology 2021Quote: Cell pellets and tumor-bearing mammary glands were homogenized in 1% SDS solution containing protease (cat# 11836153001, Roche AG) and phosphatase inhibitor cocktail (cat# 04906845001 ...
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bioRxiv - Cell Biology 2021Quote: ... transferred to PVDF membranes (Amersham Hybond) and probed with following antibodies: mouse anti-HA-HRP (1:1000) (12013819001, Roche) rabbit anti-EMC6 (1:300 ...
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bioRxiv - Cell Biology 2021Quote: ... The washed beads were resuspended in 200 μL 50 mM ammonium bicarbonate (pH 8.3) and on-bead trypsin digest (rolling with 1 μg of trypsin (Roche) for 16 h at 37°C followed by a second trypsin digest with 0.5 μg trypsin for 2 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were lysed in 1 % NP40 buffer (50mM Tris-HCl pH 7.4, 150 mM NaCl, 1 % NP40) with 2x protease inhibitor cocktail (Complete, Roche), sonicated ...
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bioRxiv - Cell Biology 2021Quote: ... Frozen pellets were thawed into 10 volumes of ice-cold hypotonic lysis buffer (0.1% SDS, 1% Triton X-100, 1x cOmplete protease inhibitor cocktail (Roche)) ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was retrieved into a new 2 ml Eppendorf tube and diluted 10 times with RIPA buffer 0% SDS (1X PBS, 1% IGEPAL-C630, 0.5% sodium deoxycholate, protease inhibitor cocktail (Roche)) to obtain a concentration of 0.1% sodium dodecyl sulfate ...
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bioRxiv - Developmental Biology 2022Quote: ... and then homogenized with a glass tissue homogenizer in lysis buffer (50 mM Tris pH 7.5, 150mM NaCl, 1% NP-40, 0.5% sodium deoxycholate) containing protease inhibitors (Roche, 11697498001). Supernatant fractions were collected by centrifugation and boiled with 2× Laemmli sample buffer (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... HA-epitope tagged recombinant proteins were detected using a rat-derived monoclonal anti-HA antibody (dilution 1:200, Roche) followed by a secondary anti-rat antibody coupled to AlexaFluor 488 fluorophores (dilution 1:200 ...