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Citations for Roche :
6201 - 6250 of 7422 citations for 6 Bromo 2 trifluoromethylimidazo 1 2 a pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: Cell line lysates were made in NP40 lysis buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1% NP40) supplemented with protease inhibitors (Roche). Next ...
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bioRxiv - Developmental Biology 2022Quote: ... Transfer membranes were then incubated with a 1:10,000 dilution of primary mouse anti-6xHis antibody (Roche, cat. #11922416001) for 1 h ...
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bioRxiv - Cell Biology 2022Quote: ... Neurons were harvested on DIV14 and lysed by addition of lysis buffer (50 mM Tris pH 8.0 and 1% SDS containing cOmplete Mini Protease Inhibitor (Roche)) and boiling at 95°C for 5 min ...
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bioRxiv - Cell Biology 2022Quote: HEK293T lysates were prepared using IP lysis buffer (150 mM NaCl, 25mM Tris-HCl pH=7.5, 1% Triton-X and protease cocktail inhibitor from Roche). Rabbit reticulocyte lysates were obtained from Promega ...
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bioRxiv - Neuroscience 2022Quote: ... Digoxigenin probes were made by standard protocols and were detected using the anti-DIG POD antibody (Roche, 1:1000) and stained using Cy3-tyramide substrate (Perkin Elmer ...
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bioRxiv - Genomics 2022Quote: ... 100 ng cDNA from each sample was subjected to quantitative real-time PCR (qPCR) following the manufacturer’s instructions of PerfectStart Green qPCR SuperMix (TransGen Biotech, #1) on LightCycle480 Instrument II (Roche). Relative mRNA expression was normalised to RPLP0 using standard statistics method of 2-ΔΔCT.
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bioRxiv - Cell Biology 2022Quote: ... and 100 µL protein breakage buffer (50 mM Tris-HCl pH 8.0, 1 mM EDTA, 20 mM Tris pH 9.5, 3 mM DTT, 1X cOmplete EDTA-free inhibitor cocktail [Roche]) were added to the samples ...
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bioRxiv - Cell Biology 2022Quote: ... diluted 10 times and 1 μl was used in 5-μl PCR reaction on a LightCycler 480 (Roche Diagnostics) with Luna Master Mix (New England Biolabs ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... pH 8.0) augmented with protease and phosphatase inhibitors (1:100 HALT PI cocktail, Thermofisher Sci Cat# 78440; or Roche cOmplete ULTRA [Cat# 05892970001] plus phosStop [Cat# 4906845001] tablets) ...
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bioRxiv - Cell Biology 2022Quote: ... 10mM Tris-HCl pH 8, 1 mM DTT, 0.2% NP-40, 1x protease inhibitor [Roche], 1x phosphatase inhibitor [Roche]), sonicated and centrifugated for 10 minutes at 14,000 RPM at 4°C ...
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bioRxiv - Neuroscience 2019Quote: Microdissections were triturated in 100 μl of lysis buffer (150 mM NaCl, 1% Triton, 100 mM Tris, pH 7.4, 10 mM EDTA, 1X protease/phosphatase inhibitor (Roche)) per 10 mg of tissue and incubated 10 min on ice ...
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bioRxiv - Developmental Biology 2020Quote: ... The resulting organoids were further digested with warm 0.25% trypsin for 1 min with constant pipetting followed by treatment with 5 mg/mL Dispase (Roche) for 5 min in at 37°C ...
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bioRxiv - Biochemistry 2019Quote: ... After 24 additional hours cells were lysed in mild detergent (10% glycerol 25mM NaCl, 20mM HEPES pH 7.0, 1% DDM, + 1x protease inhibitor (Roche)) ...
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bioRxiv - Biochemistry 2019Quote: Cells were lysed in native lysis buffer (10% glycerol, 25mM NaCl, 20mM HEPES pH 7.0) + 1%DDM and protease inhibitor (Roche) and solubilized by rotating 30m at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... and rat monoclonal anti-HA High Affinity for 60 minutes at room temperature (clone 3F10; 1:50 dilution, Roche). The sections were rinsed in PBS before incubation for 30 minutes with secondary Immunotech biotinylated antibody and 45 minutes with the Streptavidine-Peroxidase Complex (universal HRP immunostaining ...
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bioRxiv - Molecular Biology 2019Quote: ... 25 mM Tris-HCl [pH 7.5], 10 mM NaCl, 1% IGEPAL CA-630, 1mM DTT, 1.5mM MgCl2, 1X Protease inhibitor [PI, Roche # 11836170001]) and incubated on ice for 30 min ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were lysed by bead beating in FA lysis buffer + 0.5% SDS + 1 mM PMSF + EDTA-free protease inhibitor cocktail (Roche). The resulting pellets were sonicated in a Bioruptor machine ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were washed 3x with PBS and then lysed in 1 ml TX-100 lysis buffer (1% Triton X-100, 150mM NaCl, 50 mM Tris-HCl, pH 7.4) supplemented with protease inhibitor cocktail (Roche). Cell lysates were cleared by centrifugation at 10,000 × g for 10 mins at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... were incubated 15 min in lysis buffer (20 mM HEPES buffer, pH 7.0; 100 mM NaCl; 5 mM MgCl2; 1% Triton X100; protease inhibitors (Roche)) and centrifuged for 15 min at 14 000 rpm in a refrigerated microfuge ...
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bioRxiv - Bioengineering 2019Quote: ... 5% glycerol and 1 mM TCEP) and for final concentration of 0.5 mM and one complete protease inhibitor tab (Roche) was added ...
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bioRxiv - Cell Biology 2019Quote: Cells were lysed in Triton X-100 lysis buffer (1% Triton X-100 in TBS, pH 7.5) containing 10 μM Leupeptin (Roche) and 1 mM Pefabloc SC (Roche) ...
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bioRxiv - Plant Biology 2019Quote: ... cell pellets were resuspended in column buffer (20mM Tris-HCl, pH 7.5, 200 mM NaCl, 1 mM DTT, and Complete Easy protease inhibitor cocktail (Roche), lysed for 5 minutes in a water bath sonicator ...
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bioRxiv - Genomics 2019Quote: ... were lysed in 100 ul Buffer-B (50 mM Tris-HCl, pH 8.0, 10 mM EDTA, 1%SDS, 1x protease inhibitors -Roche) and sonicated in a microtube (Covaris ...
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bioRxiv - Molecular Biology 2020Quote: ... Released nuclei were washed (10 mM Tris-HCl pH 8, 200 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 1x PIC (Roche)) for 5 minutes at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... A 1 µg RNA was used to synthesize cDNA using a first-stand cDNA synthesis kit (Roche Diagnostics, Switzerland). The cDNA was normalized to 100 ng µl−1 and used for PCR analysis in a 10 µl reaction with gene-specific primers (5‘-CGTGGGATACCCTTACATGG and 5‘-CCCGATTATCTTCCACCAGA for ZmOMT1 ...
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bioRxiv - Molecular Biology 2019Quote: ... Beads were washed with Low Salt buffer (50 mM HEPES pH 7.5, 140 mM NaCl, 1% Triton X-100, 1x protease (Roche) and phosphatase inhibitors (Sigma-Aldrich)) ...
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bioRxiv - Genomics 2020Quote: ... Isolated nuclei were then lysed with lysis buffer (50mM Tris-HCl, pH 8.1, 10 mM EDTA, pH 8, 1% SDS, 1X protease inhibitor cOmplete EDTA-free cocktail, Roche). Nuclei were sonicated using a Bioruptor ultrasonic cell disruptor (Diagenode ...
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bioRxiv - Cancer Biology 2020Quote: ... pelleted at 500 × g for 10 min and lysed in TNTE buffer (1:10 wt:vol) supplemented with protease inhibitor cocktail (Roche) by homogenization using motorized Teflon-glass homogenizer (Thomas Scientific ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... 150mM NaCl, 10% glycerol, 5mM MgCl2, 0.1% Tween20, 15mM EGTA, 10μM MG132, and 1× cOmplete™ Protease Inhibitor Cocktail [Roche]) and incubated for 30 min at 8 rpm in the cold room ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.1% Triton X-100) supplemented with Complete® protease inhibitor cocktail (Roche, 1 pastel per 10 mL lysis buffer) for 5 min ...
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bioRxiv - Biochemistry 2020Quote: ... bacterial pellets were resuspended using a high salt buffer (25 mM Tris pH7.5, 1 M NaCl, 10 mM imidazole, 5% glycerol) supplemented with protease inhibitor tablets (Roche), 0.5 mM PMSF ...
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Receptor-like role for PQLC2 amino acid transporter in the lysosomal sensing of cationic amino acidsbioRxiv - Cell Biology 2020Quote: ... 1% Triton X-100 plus protease and phosphatase inhibitor cocktail tablets (Complete mini, EDTA-free, Phos-STOP from Roche). Insoluble cell material was rejected by centrifugation ...
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bioRxiv - Synthetic Biology 2021Quote: ... 0.1% SDS) and resuspension of the nuclei in 0.9 mL Covaris shearing buffer (with 1× protease inhibitors complete mini (Roche)) ...
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bioRxiv - Physiology 2021Quote: ... we collected feces of 90 adults that had fed on Arabidopsis wild type leaves for one day in a total volume of 1 mL 20 mM MES buffer pH 6.5 containing protease inhibitors (cOmplete, EDTA-free, Roche). After homogenization with metal beads for 3 min at 25 Hz in a TissueLyzer II and centrifugation at 4 °C for 10 min at 16,000 × g ...
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bioRxiv - Molecular Biology 2021Quote: ... 1.2ml of Buffer NUN2 (300mM NaCl, 7.5mM MgCl2, 1% NP-40, 1M Urea supplemented with complete EDTA free protease inhibitor (Roche) and PhoSTOP (Roche ...
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bioRxiv - Neuroscience 2019Quote: ... and harvested in 200 μl lysis buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1% Triton-X100, 1X Complete protease inhibitor cocktail [Roche] ...
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bioRxiv - Plant Biology 2020Quote: ... Pellets were resuspended in buffer (50mM Tris-HCl pH 7.0, 100 mM NaCl, and 1 tablet anti-protease cocktail (Roche) for 25 mL) ...
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bioRxiv - Molecular Biology 2019Quote: ... The supernatants were removed and aggregated proteins were washed twice with 600 μl wash buffer (20 mM Na-phosphate pH 6.8, 1 mM PMSF, 1x protease inhibitor cocktail (Complete EDTA-free, Roche), 2% NP-40 ...
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bioRxiv - Molecular Biology 2019Quote: ... washed twice in ice-cold acetone then resuspended in 200 μl ice-cold extraction buffer (50 mM Tris-HCl pH 7.5, 1 mM EDTA, 1x protease inhibitors (Complete EDTA-free, Roche), 1% SDS) ...
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bioRxiv - Developmental Biology 2020Quote: ... 2003) except that 1 to 600 dilution of anti-DIG-HRP conjugated and anti-FLU-HRP conjugated antibodies (Roche 11207733910 and 11426346910 ...
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bioRxiv - Cancer Biology 2020Quote: ... were resuspended in 50 μl PBS following the addition of 50 μl 1% SDS in 100 mM Hepes/NaOH pH8.5 supplemented with protease inhibitor cocktail EDTA-free (Roche). Samples were heated to 95°C for 5 min ...
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bioRxiv - Neuroscience 2020Quote: ... The brains were transferred to a 1.5 ml Eppendorf tube containing 500 microliters 1 mg/ml Liberase DH (Roche, prepared according to the manufacturer’s recommendation ...
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bioRxiv - Neuroscience 2019Quote: ... one series of frozen brain slices was homogenized in 1 ml PBS containing complete protease inhibitor (Roche Applied Science) and 1 mM AEBSF (Sigma ...
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bioRxiv - Neuroscience 2020Quote: Cultured cells were lysed in RIPA buffer containing protease inhibitor cocktail (1 tablet/15 mL) (Roche Diagnostics, Mannheim, Germany), sonicated and stored at −80°C until analysis ...
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bioRxiv - Molecular Biology 2019Quote: ... 200-500 mg of flower buds pooled from at least 4 individual plants were ground in liquid nitrogen or directly in 2 ml ice-cold lysis buffer (50 mM Tris HCL pH 7.5, 25 or 50 mM NaCl, 1% Triton, protease inhibitors (Complete - EDTA, Roche). After removal of cell debris by centrifugation (2 times 5 min ...
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bioRxiv - Cell Biology 2019Quote: Cells were harvested and lysed in ice-cold lysis buffer (20 mM Tris pH 8, 200 mM NaCl, 1 mM EDTA, 0.5% NP40) with protease inhibitor cocktail (Roche). Protein content of extracts was estimated using Pierce 660 nm Protein Assay Reagent (Thermo Fisher ...
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bioRxiv - Cancer Biology 2019Quote: ... 0,25 % NP-40 and supplemented with a protease inhibitor cocktail (1 tablets to 10 ml of lysis buffer, Roche) for 10 minutes at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... Q-RT pcr experiments were performed either by SYBR green or TaqMAN assay probes (Details provided in Supplementary table 1) according to manufacturer’s protocol using Lightcycler 480 II (Roche).
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bioRxiv - Molecular Biology 2019Quote: ... 150 nM each of PaaDlp-2_R1 and R2 in 1 × KAPA HiFi HotStart ReadyMix (KAPA Biosystems, Wilmington, MA, USA), and 3 µL sample eDNA ...
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bioRxiv - Genetics 2020Quote: ... Southern blot was performed according to the manufacturer’s protocol of DIG HIGH prime DNA labeling and detection starter kit 1 (Roche). 10 µg aliquot of genomic DNA was digested overnight with DraIII ...