Labshake search
Citations for Roche :
6151 - 6200 of 6565 citations for Estrone ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... genomic DNA was fragmented using a Covaris S220 ultrasonicator and libraries were generated using KAPA LTP Library Preparation Kit (Roche, KK8230). To verify that the insertion was present in the genome at the correct location in both transfected lines ...
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bioRxiv - Neuroscience 2023Quote: A total of 1.0 µg of extracted DNA was used for PCR-free library construction using the KAPA HyperPrep PCR-Free Library Prep kit (Roche, KK8505). Mechanical shearing using the Covaris microtube system (Covaris ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 µg of RNA from each sample was processed using the KAPA Stranded mRNA-Seq Kit with mRNA Capture Beads (Kapa Biosystems). Sequencing was performed using NextSeq 500 (Illumina) ...
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bioRxiv - Cell Biology 2023Quote: Libraries were pooled in equimolar amounts and the final library pool was quantified using the KAPA Library Quantification Kit for Illumina Platforms (Roche; KK4824). Libraries were sequenced on the Illumina NextSeq 500 over two NextSeq 500 High Output v2.5 150 cycle kits (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... The RNA was then extracted with the Viral NA Small volume kit (06 543 588 001, Roche Diagnostics GmbH (Mannheim, Germany)) on the MagNA Pure 96 system (Roche Diagnostics GmbH (Manheim ...
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bioRxiv - Molecular Biology 2023Quote: Automated dual indexed FFPE libraries were constructed with 50-250 ng of genomic DNA utilizing the KAPA HTP Library Kit (KAPA Biosystems) on the SciClone NGS instrument (Perkin Elmer ...
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bioRxiv - Neuroscience 2023Quote: ... All purified nucRNA (9-20 ng/sample) was used to construct cDNA sequencing libraries using the KAPA RNA HyperPrep Kit with RiboErase (KK8560, KAPA Biosystems), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The concentration of each library was accurately determined through qPCR utilizing the KAPA library Quantification Kit according to the manufacturer’s protocol (KAPA Biosystems/Roche) to produce cluster counts appropriate for the Illumina NovaSeq6000 instrument ...
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bioRxiv - Cancer Biology 2023Quote: ... and quantified using the qPCR-based quantification in order to ensure only NGS-compatible amplicon was quantified using the Library Quant ROX Low Kit (Kapa Biosystems) on a QuantStudio™ 6 Realtime PCR System (ThermoFisher) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and amplified 6 times (Sets 1 and 2) or 8 times (Set 3) with a KAPA Library Amplification Kit (KAPA Biosystems). The amplification products were cleaned using Agencourt Ampure XP reagent or KAPA Pure Beads ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries were pooled in equimolar amounts and final library quantification performed using the KAPA Library Quantification Kit for Illumina (Roche; KK4824).
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bioRxiv - Genetics 2024Quote: ... along with a single -RT sample for each corresponding biological replicate using 2 μl cDNA and a no template control (NTC) with the LightCycler® 480 SYBR Green I Master kit (Roche). Ct values were measured with the Bio-Rad C1000 CFX96 Real-Time System and Bio-Rad CFX Software (qPCR primers ...
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bioRxiv - Genomics 2024Quote: ... and then by real-time PCR against a standard curve using the KAPA Library Quantification Kit (Kapa Biosystems/Roche, Basel, Switzerland). Libraries were sequenced on an Illumina NovaSeq 6000 platform with S1 ...
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bioRxiv - Genomics 2024Quote: ... and then by real-time PCR against a standard curve using the KAPA Library Quantification Kit (Kapa Biosystems/Roche, Basel, Switzerland). Libraries were sequenced on an Illumina NovaSeq 6000 platform with S1 ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was quantified and poly(A) selected RNA-seq libraries were generated using a KAPA Stranded mRNA-seq kit (KAPA Biosystems) with 500 ng RNA per sample as starting material ...
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bioRxiv - Cancer Biology 2024Quote: ... Relative gene expression was determined using the Quanta SYBR Green Master Mix Kit (Quanta Bio) on a LightCycler 96 (Roche Diagnostics), using GAPDH as the reference gene.
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bioRxiv - Neuroscience 2024Quote: ... and fluorescein (FITC)-labeled mRNA against Dbh using reverse-transcribed mouse cDNA as a template and an RNA DIG or FITC-labeling kits from Roche (Sigma). Primer and probe sequences for the Slc17a7 (Vglut1) ...
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bioRxiv - Molecular Biology 2024Quote: ... Real-time PCR was performed to assess the concentration of target rRNA using SYBR Green Master Mix kit (Roche, Basel, Switzerland) in accordance with the manufacturer’s recommendations on LightCycler® 96 Real-Time PCR System (Roche ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries for RNAseq were prepared for each sample with a KAPA stranded mRNA-seq kit as per manufacturer’s recommendations (Roche, Indianapolis, IN); briefly ...
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bioRxiv - Neuroscience 2024Quote: ... Insert sizes were evaluated via Caliper GX (Perkin-Elmer, USA) and libraries quantitated with Kapa SYBR FAST Universal kit (Roche, USA). Sequencing was performed on the Illumina NovaSeq 6000 platform to generate 100M paired reads per sample at 150 nucleotide read lengths ...
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bioRxiv - Genomics 2024Quote: ... Final library quality was assayed using instrumentation described above as well as qPCR using the Kapa Library quantification kit for Illumina (Roche Inc.), before sequencing with other libraries on a quarter Illumina Novaseq S4 flowcell with 2*150 bp paired-end mode at the Norwegian Sequencing Centre.
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bioRxiv - Microbiology 2024Quote: ... mixed with 15 μL of a PCR mix with 1X LightCycler FastStart DNA Master Hybridisation Probes kit (Roche diagnostics, Mannheim, Germany), 0.5 U of UDG (Roche Diagnostics ...
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bioRxiv - Cancer Biology 2024Quote: ... The number of PCR cycle used for library amplification was determined by KAPA library quantification kit for Illumina platform (KAPA biosystems). Each Hi-C library was sequenced to obtain 500M reads PE100 with biological duplicates to achieve a total of 1 B reads for each condition.
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bioRxiv - Microbiology 2024Quote: ... library preparation for metagenomic analysis was conducted using the KAPA Hyper PCR-free library preparation kit (Kapa Biosystems, Roche, Basel, Switzerland), following the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2024Quote: ... library preparation for metagenomic analysis was conducted using the KAPA Hyper PCR-free library preparation kit (Kapa Biosystems, Roche, Basel, Switzerland), following the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2024Quote: ... all extracted genomes were used as the PCR templates and the sgRNA-coding sequences with iBAR were amplified using KAPA HiFi HotStart ReadyMix PCR kit (Roche, #KK2631). The DNA amplification was performed under the following condition ...
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bioRxiv - Molecular Biology 2024Quote: ... and an equimolecular pool of libraries was titrated by quantitative PCR using the “Kapa-SYBR FAST qPCR kit for LightCycler480” (Kapa BioSystems) and a reference standard for quantification ...
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bioRxiv - Microbiology 2024Quote: ... Plasma creatinine was measured in freshly thawed plasma samples using the CREP2 kit (Catalog Number 03263991190) on a Cobas 311 analyser (Roche, USA). Assays were performed by a commercial laboratory in the UK ...
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bioRxiv - Immunology 2024Quote: ... The concentration of each library was accurately determined through qPCR utilizing the KAPA library Quantification Kit according to the manufacturer’s protocol (KAPA Biosystems/Roche) to produce cluster counts appropriate for the Illumina NovaSeq6000 instrument ...
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bioRxiv - Molecular Biology 2024Quote: ... activity was measured in a kinetic assay,39 using a commercial kit (Sentinel Diagnostics 17234H) adapted for use on either a Cobas Fara or Mira analyser (Roche Diagnostics). Intra-assay and inter-assay precision were CV < 4% and CV < 8% respectively.
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bioRxiv - Cell Biology 2024Quote: ... samples were covered with the staining mixture at 37°C for 1 h following instructions of the In Situ Cell Death Detection Kit (Roche, 11684795910). After the TUNEL reaction ...
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bioRxiv - Genetics 2023Quote: ... qPCR was performed in a final volume of 20 μl containing 20 ng of total DNA using SYBR Fast Universal qPCR Kit (Kapa Biosystems) and analysed using the Quant Studio 6 Flex system (Applied Biosystems) ...
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bioRxiv - Cell Biology 2024Quote: ... Digoxigenin (DIG)-labeled anti-sense mak RNA probe was generated by in vitro transcription from this T7 promoter: mak cDNA fragment as the template using a DIG RNA labeling kit (Roche, 11175025910). Zebrafish embryos from the 1-cell stage to 72 hpf were fixed with 4% PFA at 4°C overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... size and concentration of cDNA libraries were checked using the Standard Sensitivity NGS kit Fragment Analyzer and qPCR (ROCHE Light Cycler 480). Libraries were sequenced using an Illumina Hiseq 2500 sequencer as single-end 125 base reads ...
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bioRxiv - Cell Biology 2019Quote: ... Quantitative real time polymerase chain reactions (qRT-PCR) were performed in 20 μL reactions using the FastStart universal SYBR Green master (Rox) kit (Roche diagnostics Corp.) on an Mx3000P real-time PCR machine (Agilent Technologies Inc. ...
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bioRxiv - Cell Biology 2020Quote: ... 4 µg of total RNA was used to generate libraries using of stranded poly(A) mRNA-Seq library with the KAPA stranded mRNA Library prep kit (KAPA Biosystems, KK8421). Libraries concentration was quantified with Qubit dsDNA HS (High Sensitivity ...
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bioRxiv - Microbiology 2019Quote: ... separated RNA was transferred to positively charged nylon membranes using overnight capillary transfer in 20X saline-sodium citrate (SSC) buffer as described in the DIG Northern Starter Kit manual (Roche, Cat. 12039672910).
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bioRxiv - Developmental Biology 2021Quote: ... One microgram of total RNA was reverse transcribed to cDNA using a Transcriptor First Strand cDNA Synthesis Kit (Roche Diagnostics, Tokyo Japan) using an anchored-oligo(dT)18 primer and random hexamer primer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Digoxigenin (DIG)- and biotin-labelled antisense probes of target IRs were transcribed from linearized pCS2+ vectors with the coding region of the corresponding receptors using the T7 RNA transcription kit (Roche, Mannheim, Germany). Labelled probes were subsequently fragmented to an average length of about 800 bp by incubation in sodium carbonate buffer (80 mM NaHCO3 ...
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bioRxiv - Developmental Biology 2021Quote: ... The digoxigenin (DIG)-labeled sense or antisense RNA probe for full-length Emi2 was synthesized with a DIG-RNA-labeling kit (Roche Molecular Biochemicals). No signal was detected with sense probes ...
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bioRxiv - Developmental Biology 2021Quote: ... The DIG-labeled 3’UTR of Emi2 and cyclin B1 RNA probes were synthesized with a DIG-RNA-labeling kit (Roche Molecular Biochemicals). Two μg of probes was incubated with purified Flag-tagged proteins for 15 min ...
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bioRxiv - Physiology 2022Quote: ... was performed on the cDNA using the Sybr green method of quantification on a Roche Lightcycler 480 using a SYBR FAST quantitative PCR kit (Kapa Biosystems; KK4611). Gene expression was analyzed for each sample in triplicate using the forward and reverse primers indicated on Table 1 ...
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bioRxiv - Neuroscience 2022Quote: ... and PlxnA4 expression levels were measured by qRT-PCR reaction using KAPA SYBR FAST ABI prism qPCR kit (Kapa Biosystems, catalog #KK4605). Primers (5’3’ ...
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bioRxiv - Genomics 2020Quote: ... a total of 100 ng DNA from each bacterial strain was used according to KAPA Hyper Prep kit (KAPA Biosystems, MA, USA) at the Georgia Genomics and Bioinformatics Core (GGBC) ...
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bioRxiv - Genomics 2020Quote: Each of the 10 plate libraries was quality assessed on an Agilent Bioanalyzer 2100 High Sensitivity chip and quantified by qPCR using the Illumina Kapa Library Quantification Kit (Roche, Cat. #KK4824). The libraries were then normalized to a concentration of 4 nM and pooled for sequencing ...
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bioRxiv - Genomics 2020Quote: ... Hybridization of the digoxegenin (DIG)-labelled 31bp oligonucleotide probe and detection was carried out following the DIG High-Prime Labelling and Detection Starter Kit (Roche SKU 11745832910).
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bioRxiv - Microbiology 2020Quote: ... Total RNA was collected 96 hours post-infection (hpi) and RNA was extracted with the High Pure RNA Isolation kit (Roche, cat#11828665001), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... was used to construct DNA libraries for sequencing on the Illumina platform using the KAPA Library Preparation Kit (Kapa Biosystems, Woburn, MA). DNA was fragmented with the Covaris E210 or E220 to ∼200 bp ...
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bioRxiv - Microbiology 2019Quote: The pooled library was prepared with 10 μl of barcoded PCR bead purified product from each individual sample and measured with KAPA qPCR library quantification kit (KAPA Biosystems, USA), before being run on an Illumina Mi-Seq Sequencer using a 600-cycle pair end reagent kit (Mi-Seq Reagent Kits v2 ...
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bioRxiv - Microbiology 2019Quote: ... The DNA from the oral samples and from the skin samples from the back were isolated using the MagnaPure LC DNA Isolation Kit III (Bacteria, Fungi; Roche, Mannheim, Germany) as described by Santigli et al ...