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• Transcription factor-like 5 is a potential DNA/RNA-binding protein essential for maintaining male fertility in mice

  Weiya Xu, et al., bioRxiv - Cell Biology 2021

  Quote: ... The pellet was washed in buffer A three times and then homogenized in 400 ul of buffer B (250 mM sucrose, 10 mM Tris HCl [pH 8.0], 10 mM MgCl2, 1 mM EGTA, 1X protease-inhibitor cocktail [11697498001, Roche], 0.1% Triton X-100, and 0.25% NP-40) for 10 min at 4°C ...