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Citations for Roche :
551 - 600 of 6811 citations for Recombinant Human Collagen Type II Alpha 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... the cells were harvested from the matrices by digesting the collagen with liberase DL research grade (24 U; Roche, Basel, Switzerland). The cells were then fixed with 4% PFA ...
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bioRxiv - Bioengineering 2024Quote: ... the cells were harvested from the matrices by digesting the collagen with liberase DL research grade (24 U; Roche, Basel, Switzerland). The cells were then fixed with 4% PFA ...
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bioRxiv - Immunology 2021Quote: Secreted twin strep-tagged gp140-soc proteins in the harvested and filtered supernatant (1L) were supplemented with protease inhibitor tablets (Roche Diagnostics) to prevent protein degradation and 5 ml of BioLock biotin blocking solution (iba Life Sciences GmbH ...
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bioRxiv - Genetics 2020Quote: N-terminal HA-tagged PRUNE1 overexpression constructs were transfected into PRUNE1 non-expressing HEK293 cells using X-tremeGENE HP DNA (Roche; 06366236001) transfection reagent ...
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bioRxiv - Immunology 2022Quote: ... filtered conditioned medium was loaded onto a 5 ml cOmplete™ His-tag Purification Column (Roche Diagnostics, Vilvoorde, Belgium). The protein was eluted with 250 mM imidazole in HBS after which the imidazole was removed using a HiPrep™ 26/10 Desalting column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... The clarified cell lysate was bound to 10 mL of cOmplete His-Tag purification resin (Roche, Germany, 30 min), washed with 1 L base buffer ...
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bioRxiv - Biophysics 2020Quote: ... The supernatant was clarified by a 5 µm filter followed by a 0.45 µm filter and loaded on a cOmplete His-Tag purification Column (Roche) with a column volume (CV ...
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bioRxiv - Molecular Biology 2019Quote: ... Purified products were subjected to second strand synthesis using 0.3 µM of second strand biotinylated primer and the KAPA Hi-Fi hot start ready mix (KK2601, Roche). The second strand reaction was carried out at 95 °C for 3 minutes ...
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bioRxiv - Genomics 2020Quote: ... The quality and quantity of the capture Hi-C libraries was tested by Bioanalyzer and KAPA qPCR (Kapa Biosystems). Capture Hi-C libraries were analysed by 75 bp paired-end Next Generation Sequencing on an Illumina NextSeq500 (My-La ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified supernatant was filtered through a 0.45 μM PES syringe filter and incubated with 10 mL of cOmplete His-Tag purification beads (Roche) for 1 h with gentle rocking ...
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bioRxiv - Molecular Biology 2023Quote: ... then subject to second-strand synthesis using 1.5 μl biotinylated oligo (10 μM) and 25 μl KAPA Hi-Fi hot start ready mix 2x (KK2601, Roche). Second strand reactions were incubated at 95°C for 3 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... was purified via Immobilized Metal Affinity Chromatography (IMAC) using a prepacked 5 mL cOmpleteTM His-Tag purification column (Roche) equilibrated in HEPES-buffered saline buffer (HBS ...
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bioRxiv - Neuroscience 2023Quote: ... Protein was eluted with wash buffer containing 10 mM maltose and subsequently bound to c0mplete His tag resin (Roche) for 2 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Cleared lysate was then used in a batch purification protocol by addition of cOmplete His-Tag resin (Roche; 5893682001) for 1 hr at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Expressed proteins in the cell-culture supernatant were purified using a cOmplete His-Tag Purification Resin (Roche, Cat# 5893682001) affinity column ...
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bioRxiv - Microbiology 2023Quote: ... Expressed proteins in the cell-culture supernatant were purified using a cOmplete His-Tag Purification Resin (Roche, Cat# 5893682001) affinity column ...
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bioRxiv - Microbiology 2020Quote: ... 400 μl sonication solution and 3.8 μg/μl of proteinase K (proK, Recombinant PCR Grade [Roche]) for fraction B2 ...
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bioRxiv - Microbiology 2022Quote: DNase treatment was performed according to the manufacturer’s protocols (RNase-free DNase I recombinant, Roche, US) for 1 hour ...
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bioRxiv - Genetics 2021Quote: ... all RNA samples used in this study were treated with recombinant RNase-free DNase I (Roche) (10 U of DNase I per 3 mg of total RNA ...
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bioRxiv - Microbiology 2020Quote: ... the recombinant RBD007 plasmid was transfected using FuGENE 6 transfection reagents (Roche Applied Science, Indianapolis, IN) into CHO-K1 cells precultured in F-12K medium (American Type Culture Collection ...
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bioRxiv - Bioengineering 2021Quote: ... pastoris culture medium containing the recombinant protein was purified by affinity chromatography Ni-NTA agarose (Roche).
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bioRxiv - Cell Biology 2020Quote: ... Chloroform extracted RNA was treated with RNase free recombinant DNase I (047 716 728 001, Roche) for 1 h at 37°C using 2 units/μg of RNA ...
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bioRxiv - Bioengineering 2023Quote: ... coli DH10B recombinant strain was extracted using the High Pure Plasmid Purification Kit (Roche, Basel, Switzerland), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The embryos were incubated in 50 μL of recombinant Proteinase K (Roche Holding AG, Basel, Switzerland) diluted to ∼2 mg/mL in 1 x tris-EDTA (TE ...
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bioRxiv - Cancer Biology 2021Quote: Total cell lysates from human RMS cell lines and human myoblasts were obtained following lysis in RIPA lysis buffer supplemented with protease inhibitors (Roche). Western blot analysis was performed similar to Ignatius et ...
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bioRxiv - Plant Biology 2019Quote: ... (2010) using a LightCycler 480 II instrument (Roche Applied Science). The housekeeping genes EXP (At4g26410 ...
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bioRxiv - Immunology 2019Quote: ... supplemented with 2% FCS and 1mg/mL dispase II (Roche) overnight at 4°C ...
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bioRxiv - Immunology 2019Quote: ... qPCR was carried out on a Lightcycler 480 II (Roche) using Taqman validated FAM-labeled probes and Gene Expression Master Mix (Applied Biosystems) ...
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bioRxiv - Neuroscience 2021Quote: ... DNase I grade II from bovine pancreas (Roche, Cat#10104159001) was dissolved in 1x HBSS at 10 mg/ml (10x ...
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bioRxiv - Neuroscience 2020Quote: ... containing phosphatase inhibitor cocktail II (Roche, Neuilly sur Seine, France) and protease inhibitors cocktail (Roche) ...
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bioRxiv - Cell Biology 2021Quote: ... and a LightCycler® II real-time PCR system (Roche). Dissociation curve analysis of the amplified DNA melting temperature showed that each primer set gave a single and specific product (Sun et al. ...
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bioRxiv - Cell Biology 2021Quote: ... 5 mg DNase I (from Bovine Pancreas grade II, Roche) was added to 50 mL of lysate ...
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bioRxiv - Microbiology 2021Quote: ... All reactions were performed using a LightCycler 4800 II (Roche) with 0.4 µM of each primer and 5 ng of template RNA (10 µl total volume) ...
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bioRxiv - Cell Biology 2022Quote: ... then incubated with 1.2 U/ml dispase II (Roche Diagnostics) in Roswell Park Memorial Institute 1640 medium (RPMI ...
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bioRxiv - Microbiology 2022Quote: ... and counter-stained with Hematoxylin II (Roche cat #790-2208) for 4 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... on a LightCycler 480 II PCR system (Roche, Basel, Switzerland). Expression was normalized to the mean of two housekeeping genes Rps13 (Ribosomal Protein S13 ...
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bioRxiv - Microbiology 2022Quote: ... with a LightCycler 480 System II (Roche Diagnostics, Mannheim, Germany). A serial dilution series of the positive control DNA for each kit were used to generate calibration curves to determine the copy number of the BLV provirus ...
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bioRxiv - Neuroscience 2022Quote: ... RT-qPCR was performed on a LightCycler 480 II (Roche) using LightCycler 480 SYBR Green I Master mix (Roche ...
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bioRxiv - Evolutionary Biology 2019Quote: ... chemistry in combination with the Lightcycler 480 II system (ROCHE). PCR reaction volumes of 10 μL contained ...
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bioRxiv - Immunology 2020Quote: ... deoxyribonuclease I from bovine pancreas grade II (Roche, IN, USA), and trypsin inhibitor type II-S soybean ...
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bioRxiv - Cell Biology 2019Quote: ... and collagenase and dispase II (catalog number: 04942078001, Roche®) solution (100 units/mL and 2 units/mL ...
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bioRxiv - Immunology 2019Quote: ... grade II and 4 mg collagenase A (Roche Applied Science) and placed in a 37°C water bath for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... The Cell Proliferation Kit II (XTT) was supplied from Roche, Inc ...
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bioRxiv - Molecular Biology 2020Quote: ... and measured using the LightCycler® 480 Instrument II (Roche). Gene-specific primers were synthesized according to the sequences in Supplemental Table S5 ...
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bioRxiv - Cancer Biology 2021Quote: ... Real-time PCR was performed (Roche LightCycler® 480 II) using a prime time gene expression master mix (IDT ...
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bioRxiv - Cancer Biology 2020Quote: ... qPCR was performed on the Lightcycler 480 instrument II (Roche) using TaqMan primer/probe sets ...
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bioRxiv - Cancer Biology 2020Quote: ... qPCR was performed on the Lightcycler 480 instrument II (Roche) using primer/probe sets ...
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bioRxiv - Cell Biology 2020Quote: ... digested with dispase II(0.1-0.5 U/ml, Roche, 04942078001) in a 33 □ water bath for 3~8 min to obtain single cells or islet cell clusters ...
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bioRxiv - Cancer Biology 2021Quote: ... with a Light Cycler 480 II instrument (Roche, Basel, Switzerland). The primer sequences are listed in Supplementary Table S2 ...
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bioRxiv - Immunology 2020Quote: ... on a LightCycler® 480 Instrument II (Roche Life Science). Primer sequences are provided in Supplementary Table 7.