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Citations for Roche :
551 - 600 of 879 citations for Mouse ONECUT3 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Transfections and antibody staining were performed as follows: two micrograms plasmid DNA was transfected using Xtremegene HP transfection reagent (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were transfected with 1 ug of shRNA/cDNA and 1 ug of viral packaging plasmids (250ng pMD2.G and 750ng psPAX2) using 6 ul of Xtreme Gene 9 transfection reagent (Roche). After 24 hr ...
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bioRxiv - Genetics 2021Quote: Overexpression plasmids were respectively transfected into U251-APP cells by using the X-tremeGene HP DNA transfection reagent (ROCHE, 6366236001). Cells grown in 6-well plate were transfected by overexpression plasmid of the target gene or its corresponding control plasmid (each 2 μg ...
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bioRxiv - Biochemistry 2021Quote: For transient transfection cells were seeded and after 18 h cells were transfected with plasmid DNA using X-tremeGENE HP DNA transfection reagent (Roche), according to the manufacturer’s protocol (for 12-well plate format ...
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bioRxiv - Biochemistry 2021Quote: ... and pPB-tetIRB-tetON-BSD plasmid bearing CLPTM1L-mEGFP or pPB-tetON-BSD-tightFF plasmid bearing CLPTM1L-FLAG-6His using X-tremeGENE 9 DNA transfection reagent (Roche), and incubated at 37 °C for 2 days ...
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bioRxiv - Biochemistry 2021Quote: ... 2×106 cells HEK293T cells were transfected with 2 μg plasmid DNA using X-tremeGENE HP DNA Transfection Reagent (Roche) in 10-cm dishes ...
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bioRxiv - Bioengineering 2020Quote: ... Hela cells were plated one day before in 6-well plate at 50% confluency and transfected with 1 μg plasmid and 3 μl XtremeGeneHP transfection reagent (Roche) during 24 hours ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids were digested in 50-100 μl total volumes with restriction enzymes and their appropriate buffer (ether Roche or NEB) in accordance with the manufacturers recommendations (typically 1 μg of DNA was digested with 1 unit of enzyme for 1 hour at 37°C) ...
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bioRxiv - Microbiology 2021Quote: ... and transfected with plasmid pIIIH5red-SARS-2-S DNA using X-tremeGENE HP DNA Transfection Reagent (Roche Diagnostics, Penzberg, Germany) according to the manual ...
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bioRxiv - Developmental Biology 2021Quote: PCR products were cloned in pBlueScript KS+ vector and digoxigenin-labelled probes were generated from linearized plasmids using RNA-labeling mix (Roche). Whole-mount chromogenic in situ hybridization was performed as described previously (Marchal 2009 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then transiently transfected with MaMTH bait (MMD or MBOAT7) and prey (MMD, MBOAT7 or PEX7 negative control) plasmids using X-tremeGENE9 DNA transfection reagent (Roche) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Both Caco2 and HepG2 cells were transfected with 1 µg of pooled plasmid using X-tremeGENE9 Transfection Reagent (Roche:6365779001) following manufacturer recommendations ...
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bioRxiv - Molecular Biology 2022Quote: ... Each dish was transfected with 400 ng of the reporter plasmid using X-tremeGENE HP DNA transfection reagent (Roche, 6366236001), at a ratio of 400 ng DNA to 40 µL 37 °C serum-free DMEM to 1.6 µL reagent per dish being transfected ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were transfected with 1 µg of shRNA/cDNA and 1 µg of viral packaging plasmids (250 ng pMD2.G and 750 ng psPAX2) using 6 µl of Xtreme Gene 9 transfection reagent (Roche). After 24 hours of transfection ...
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bioRxiv - Neuroscience 2022Quote: ... Antisense and sense RNA probes were synthesized from the linearized plasmid template by in vitro transcription using a DIG RNA Labeling Kit (Roche). In situ hybridization was performed on cryo-sections of telencephalon as previously described (Masai et al. ...
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bioRxiv - Genomics 2024Quote: ... Cells were then transfected with the Tet-pLKO-puro plasmids targeting either UHRF1 or DNMT1 transcripts and the accompanying lentiviral packaging (psPAX2) and envelope (pMD.2G) plasmids with Opti-MEM and Xtreme HP Gene transfection reagent (Roche) per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... grown for 24 hours and transfected with 8 μg of plasmids and 8 μl of X-tremeGENE HP DNA Transfection Reagent (Roche). The cells were grown for 48 hours post-transfection and collected for downstream analyses.
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bioRxiv - Developmental Biology 2023Quote: ... Purified plasmids were used to prepare digoxigenin-labelled sense and anti-sense riboprobes using T7 and SP6 RNA polymerases (Roche). Probes were purified using ethanol precipitation and resuspended in 1:1 volume of DEPC-treated water:formamide.
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bioRxiv - Bioengineering 2023Quote: ... The construct was synthesized by GenScript® and the plasmid was used as template for PCR amplification (Kapa Hifi Hotstart Ready Mix, Cat#F-530-L, Roche). The PCR amplicon was purified with NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel ...
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bioRxiv - Cell Biology 2023Quote: Parental and Haspin knockout cells stably expressing GFP-TAF5 were obtained by cotransfection with pcDNA5/FRT/TO/GFP-TAF5 (Antonova et al., 2018) and a pPGKpuro resistance plasmid (in a 9:1 ratio) using Xtreme Gene 9 (Roche). After 48 h ...
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bioRxiv - Developmental Biology 2023Quote: ... 1μg linearized plasmid was used for In Vitro transcription to make DIG labelled RNA probe (DIG RNA Labeling Mix-11277073910 Roche). Whole mount RNA in situ hybridization was performed by following the standard zebrafish protocol (http://zfin.org/ZFIN/Methods/ThisseProtocol).
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bioRxiv - Developmental Biology 2022Quote: ... Plasmids were linearized and probes were prepared as described in Wilkinson & Nieto (1993) using digoxigenin-conjugated nucleotides (Roche, Basel, Switzerland). Sense probes were used to confirm specificity of the antisense probes ...
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bioRxiv - Cell Biology 2024Quote: ... RPE1 cells were transfected with PB-Tet-LAP-MAP3K1 and PB-Transposase plasmid (kindly provided by N. Dimitrova) using X-tremeGENE9 reagent (Roche) and after 48 hours cells were selected with G418 ...
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bioRxiv - Microbiology 2023Quote: ... a 2.3 kb-homologous DNA fragment was cloned into a unique SpeI site of a non-replicative plasmid (pIJ10700) using T4 DNA ligase (Roche). The fragment was amplified using Q5® DNA polymerase (NEB ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK-293T and MEF cells (except for p62 KO MEF cells) were transfected with plasmids using X-tremeGENE 9 (Roche) as described as previously (41–43) ...
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bioRxiv - Developmental Biology 2024Quote: ... Digoxigenin (DIG)-labeled antisense and sense RNA probes for whole-mount in situ hybridization (WISH) were transcribed from these plasmids using T7 or SP6 polymerases (Roche).
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bioRxiv - Developmental Biology 2024Quote: ... and 2.28 μg of the transfer plasmid was vortexed with 11.9uL X-tremeGENE™ HP DNA Transfection Reagent (Roche; 6366244001) and 360 μl OptiMEM (GIBCO ...
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bioRxiv - Synthetic Biology 2021Quote: ... and then incubated with mouse anti-GFP monoclonal antibody(1:1000; Roche, Indianapolis, IN) as well as rabbit anti-alpha-tubulin polyclonal antibody (1:1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... Tail genomic DNA was extracted using a KAPA mouse genotyping Kit (KAPA Biosystems KK7352), and PCR was performed using primers as described in Supplementary Table 1 ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibody dilutions for immunostaining were mouse anti-GFP (Roche IgG1κclones 7.1 and 13.1) 1:200 ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA was extracted from tail tissue with a KAPA Mouse Genotyping Kit (KAPA Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... and anti-rabbit/mouse IgG secondary antibody (1:2000; Roche, Cat#12015218001, Basal, Switzerland) prior to fluorescent imaging ...
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bioRxiv - Cell Biology 2019Quote: Immunoprecipitation was performed as previously described using mouse monoclonal anti-GFP antibodies (Roche, 11814460001) that had been pre-conjugated with Protein-G magnetic beads (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: The following antibodies were used for immunofluorescence staining: mouse anti-HA (1:500, Roche), and goat-anti-mouse Alexa 488 (1:400 ...
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bioRxiv - Cell Biology 2019Quote: ... Primary antibodies including rat anti-tubulin (MCA78G; Serotec) and mouse anti-Myc (11667149001; Roche) were used at a dilution of 1:5000 and 1:200 ...
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bioRxiv - Cell Biology 2021Quote: We immunoprecipitated EGFP-labeled imaginal disc nuclei with mouse anti-EGFP (Roche, cat. #11814460001). For immunofluorescence ...
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bioRxiv - Neuroscience 2021Quote: ... cells were isolated from mouse brains with Collagenase D (0,5 mg/ml, #11088866001, Roche), DNase I (10μg/ml ...
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bioRxiv - Plant Biology 2021Quote: ... GFP-tagged proteins were detected with a mouse anti-GFP antibody (1:5000; Roche). Immunoblot results were quantified using Image J software (v1.8.0).
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bioRxiv - Microbiology 2021Quote: ... Western-blot antibody detection was used using antibodies from Roche Diagnostics Mannheim Germany (Anti-HA, mouse monoclonal primary antibody (12CA5 Roche, 5 mg/ml) at a dilution of 1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... The primary antibodies used in this study were mouse anti-HA (1:2000, Roche) and anti-GAPDH (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... Mouse tail epidermis was separated from dermis by 5 mg/ml Dispase II (Roche) digestion in 37°C for 1 hr ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit polyclonal anti- GFP (gift from Oliver Gruss) and mouse monoclonal anti-GFP (Roche), mouse monoclonal anti-β actin (Sigma) ...
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bioRxiv - Biochemistry 2020Quote: ... GFP-tagged proteins were detected with a primary mouse antibody IgG1K Anti-GFP (Roche) diluted to 1:1000 in 5 % (w/v ...
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bioRxiv - Genetics 2022Quote: ... Primary antibodies used in cytological analyses were: mouse anti-GFP 1:500 (Roche, #11814460001), mouse anti-HA 1:600 (Biolegend ...
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bioRxiv - Immunology 2019Quote: Mouse lungs were homogenized in HEPES buffer with Liberase Blendzyme 3 (70ug/ml; Roche) and DNaseI (30ug/ml ...
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bioRxiv - Molecular Biology 2019Quote: ... and a Cy3-labeled goat anti-mouse IgG secondary antibody (Roche; 1:100 dilution). Samples were mounted using SlowFade Diamond Antifade with DAPI mounting media (Invitrogen ...
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bioRxiv - Genetics 2019Quote: ... Cells were lysed and cleared extracts incubated with Mouse anti-MYC (9E10) antibodies (Roche) to immunoprecipitate MYC epitope tagged proteins as previously described (Bloom et al. ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies included mouse monoclonal anti-GFP (1:200; Roche Applied Science, Cat#11814460001), and rabbit polyclonal anti-Ds-red (1:200 ...
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bioRxiv - Pathology 2020Quote: ... DNA was extracted from tail tissue using the KAPA Mouse Genotyping Kit (KAPA Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Antibodies were used at the following concentrations: mouse anti-GFP monoclonal (1:100; Roche), rabbit anti-Pav polyclonal (1:250 ...