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Citations for Roche :
551 - 600 of 6176 citations for HCV 1 e2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... CaCl2 was added to a final concentration of 1.25 mM and incubated with 200 μl Anti-Protein C Affinity Matrix (Roche) for 4 hrs while rotating at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... Whole cell extracts were prepared after transfection and incubated with indicated antibodies together with Protein A/G beads (Roche) overnight ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were harvested and ruptured with a Dounce homogenizer in the buffer A containing cOmplete protein inhibitor cocktail (Roche) and 1mM phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were obtained by lysing cells in PBS containing 0.1% Triton X-100 (PBS/TX) as well as protease and protein inhibitor cocktails (Roche) for 5 min on ice ...
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bioRxiv - Biochemistry 2022Quote: ... The cell lysate was diluted to 0.8 mg/ml total protein concentration and 0.47% NP40 using dilution buffer (PBS plus protease inhibitor cocktail (Roche 04693159001) and precleared using Invitrogen™ Dynabeads™ MyOne™ Streptavidin T1 magnetic beads for 2-4 hours at 4°C during gentle rotation ...
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bioRxiv - Biochemistry 2022Quote: ... EDTA-free TCA buffer was used where phosphorylation of proteins was to be investigated by alkaline phosphatase-treatment (Roche) or Phos-tagTM (NARD Institute ...
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bioRxiv - Neuroscience 2022Quote: ... Lysate was precleared with Pierce™ Protein G Agarose and immunoprecipitated with anti-GFP antibodies (Roche Cat. No. 11814460001) bound on protein G agarose ...
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bioRxiv - Microbiology 2023Quote: ... Expressed proteins in the cell-culture supernatant were purified using a cOmplete His-Tag Purification Resin (Roche, Cat# 5893682001) affinity column ...
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bioRxiv - Neuroscience 2023Quote: ... Protein was eluted with wash buffer containing 10 mM maltose and subsequently bound to c0mplete His tag resin (Roche) for 2 hours ...
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bioRxiv - Cancer Biology 2023Quote: Protein were isolated by using RIPA buffer with protease inhibitor cocktail (cOmplete Protease Inhibitor Cocktail, Roche, Mannheim, Germany, 04693116001), and concentrations were measured using the Qubit Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Pathology 2023Quote: ... Approximately 30-50 μg of proteins were separated by 10% SDS-PAGE and transferred onto a PVDF membrane (Roche). The membrane was blocked with 5% non-fat milk in PBS for 1 h at room temperature and then incubated with the specific primary antibodies at 4°C overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... Whole cellular protein was extracted with RIPA buffer (Serva electrophoresis) containing protease inhibitor cocktail and phosphatase inhibitor cocktail (Roche). Western blots were performed following routine protocols55 ...
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bioRxiv - Microbiology 2023Quote: ... Expressed proteins in the cell-culture supernatant were purified using a cOmplete His-Tag Purification Resin (Roche, Cat# 5893682001) affinity column ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative Western blot analysis was employed to determine concentration of recombinant protein using a recombinant GFP standard (Roche, 11814524001) with a defined concentration of 1 mg/ml ...
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bioRxiv - Cancer Biology 2024Quote: Total proteins were extracted from cultured NB cells using RIPA buffer supplemented with protease and phosphatase inhibitor cocktail (Roche). The cell lysates were pre-cleared with protein A or protein G beads (Santa Cruz Biotechnology ...
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bioRxiv - Biochemistry 2021Quote: ... 137.5 mM NaCl, 1 mM EDTA, 1% Triton X-100, 1 mM sodium fluoride, EDTA-free protease inhibitor cocktail [Roche]). 1 mM orthovanadate was added to the lysis buffer to prevent binding of substrates to the PTP1B trapping mutant ...
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bioRxiv - Developmental Biology 2021Quote: Tissue samples were minced into small pieces (1-3 mm3) using a scalpel and dissociated with collagenase/dispase (1 mg mL-1; COLLDISP-RO, Roche) in the presence of Rock inhibitor (RI ...
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bioRxiv - Cancer Biology 2019Quote: Cells were lysed in PBS-E LB (1x PBS, 1% TX100, 1 mM EDTA, 1 mM DTT, Protease Inhibitor cocktail (Roche) and Phosphatase Inhibitor solution (Sigma)) ...
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bioRxiv - Cell Biology 2021Quote: ... Pellets were lysed in 300 µL FA lysis buffer (50 mM HEPES–KOH pH 8.0, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 1 mM PMSF, Roche protease inhibitor) with ∼1 mL ceramic beads using a Fastprep-24 (MP Biomedicals) ...
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bioRxiv - Cell Biology 2021Quote: ... Heads were homogenised in 1 mL BRB-80 lysis buffer (80 mM PIPES, 1 mM MgCl2, 1 mM EGTA, 1 mM DTT, cOmplete EDTA-free protease inhibitor cocktail [Roche] ...
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bioRxiv - Cell Biology 2021Quote: ... Pellets were lysed in 300 µL FA lysis buffer (50 mM HEPES–KOH pH 8.0, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 1 mM PMSF, Roche protease inhibitor) with ∼1 mL ceramic beads on a Fastprep-24 (MP Biomedicals) ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM MgCl2, 10% glycerol, 1% Tween-20, 20 mM imidazole, 1 mg/ml lysozyme, and 1x protease tablet [Roche]) for thirty minutes on ice with 5 min intervals of 10 s vortexing ...
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bioRxiv - Molecular Biology 2020Quote: Cells were lysed in lysis buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 1% Nonidet P-40, 1 mM sodium orthovanadate and 1× complete protease inhibitor cocktail from Roche) at 4°C for 15 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... 10 mM Imidazole pH 8) and supplemented with PMSF (1:100), protease-inhibitor mix HP Plus (1:500, Serva) and DNaseI (1:300, Roche), lysed by sonication and cleared by centrifugation at 108000g for 45 min ...
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bioRxiv - Molecular Biology 2022Quote: Pellets were thawed and lysed in 300 µl FA lysis buffer (50 mM HEPES–KOH pH 8.0, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 1 mM PMSF, Roche protease inhibitor) with ∼1 ml ceramic beads on a Fastprep-24 (MP Biomedicals) ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatants were diluted 1:4 with ice-cold dilution buffer (1× phos-stop [Roche], 0.5% NP-40, 1 mM DTT) and centrifuged again at 15,000 rpm for 15 minutes at 4°C to precipitate actomyosin components ...
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bioRxiv - Plant Biology 2022Quote: ... 150 mM NaCl, 1 mM EDTA, 10% glycerol, 1% Triton X-100, 1 mM PMSF, 1x cOmplete protease inhibitor cocktail from Roche, 1x phosphatase inhibitor cocktail 2 and 3 from Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... expression vectors were co-transfected into HEK293T cells with the lentiviral packaging constructs psPAX2 and pMD2.G (VSV-G) in a 1:1:1 ratio using X-tremeGENE9 DNA Transfection Reagent (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The remaining extract was centrifuged at 2,000 rpm for 5 minutes and re-suspended in 1 x PBS Lysis buffer (1 x PBS, 0.01% NP-40, 5% Glycerol, 150mM NaCl, 1 x Roche cOmplete), sonicated and stored at -20°C for downstream applications.
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bioRxiv - Cell Biology 2023Quote: Decapsulated testis extract was re-suspended into 1 x PBS Lysis buffer (1 x PBS, 0.01% NP-40, 5% Glycerol, 150mM NaCl, 1 x Roche cOmplete) and sonicated for 20 s at 22% amplitude in cycles of 0.4 s on and 0.2 s off ...
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bioRxiv - Biochemistry 2023Quote: ... α-Ape1 (1:5000) (Hamasaki et al., 2003), α-Prb1 (laboratory stock, 1:5000) and α-GFP (Roche, 11814460001, 1:1000), were used as primary antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... ssDNA deamination activities were measured using WCE prepared using 100 μL HED lysis buffer per 1 million cells (25 mM HEPES, 15 mM EDTA, 10% Glycerol, 1 mM DTT, and 1 protease inhibitor tablet [Roche]). Samples were sonicated in a water bath sonicator to ensure complete lysis ...
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bioRxiv - Cell Biology 2023Quote: ... 50 mM NaF, 1 mM EDTA, 1 mM EGTA, and 1% Triton X-100, supplemented with Complete EDTA-free protease inhibitor [Roche]). Cell samples were rotated for 10 min at 4 °C to achieve lysis ...
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bioRxiv - Developmental Biology 2023Quote: Spinal cords from wt and Sorcs2-/- mice were dissected and lysed on ice with TNE lysis buffer (Tris-base 10 mM, NaCl 150 mM, EDTA 1 mM, NP40 1%, cOmplete 1% (Roche), PhosSTOP 1% (Roche) ...
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bioRxiv - Neuroscience 2023Quote: ... 1% (w/v) sodium deoxycholate and 1% (v/v) Triton X-100) with 1% (v/v) protease inhibitor cocktail (Roche). After homogenization ...
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bioRxiv - Cell Biology 2023Quote: ... 2 M urea, 0.5% SDS, 1 mM EDTA pH8.0, 1 mM DTT, and 1× protease inhibitor cocktail [Roche, cat#4693132001]). Biotinylated RBPs were captured by affinity purification ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mM MgCl2, 0.5 mM β-mercaptoethanol, 1 mM ATP, 1% IGEPAL, 5% glycerol, 3 U/ml Benzonase and Roche Complete EDTA-free protease inhibitor ...
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bioRxiv - Cancer Biology 2024Quote: ... expression vectors were co-transfected into HEK293T cells with the lentiviral packaging constructs psPAX2 and pMD2.G (VSV-G) in a 1:1:1 ratio using X-tremeGENE9 DNA Transfection Reagent (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 1% Triton-x-100 containing 1 EDTA-free protease inhibitor tablet (Roche) and 1% phosphatase inhibitor cocktails 1 and 2 (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM PMSF and 1 Complete Mini Protease Inhibitor Cocktail tablet (Roche) were added to 10 mL buffer ...
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bioRxiv - Microbiology 2020Quote: ... anti-3xHA (1:2000, 12CA5, Roche or 1:4000, C29F4, Cell Signaling) anti-PfALD (1:1000 ...
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bioRxiv - Cell Biology 2019Quote: ... 1° antibodies used: α-GFP 1:1000 dilution (Mouse, Roche, Cat.No. 11814460001), α-Tubulin (TAT1 ...
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bioRxiv - Biochemistry 2021Quote: ... 1 % (v/v) NP40 and 1 x cOmplete Protease inhibitor cocktail (Roche)) by incubating on ice for 30 min ...
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bioRxiv - Genomics 2021Quote: ... with a 1:1 ratio of Kapa Pure beads (Roche, Basel, Switzerland) and eluted in Tris-Cl ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mM EDTA and supplemented with 1 × complete protease inhibitor cocktail (Roche). DDM/CHS detergent (1%/0.1% ...
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bioRxiv - Cell Biology 2022Quote: ... incubated overnight with primary antibodies (1:5000 α-TNY1, 1:10,000 Roche Rat-anti-HA ...
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bioRxiv - Microbiology 2022Quote: ... 10U ml−1 DNase 1 and Complete Protease Inhibitor Cocktail Tablet (Roche)) ...
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bioRxiv - Microbiology 2022Quote: ... DNase 1 10U mL−1 and Complete Protease Inhibitor Cocktail Tablet (Roche)) ...
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bioRxiv - Microbiology 2022Quote: ... 10U mL−1 DNase 1 and Complete Protease Inhibitor Cocktail Tablet (Roche)) ...
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bioRxiv - Immunology 2023Quote: ... 1% Triton X-100) containing 1×EDTA-Free Protease inhibitor cocktail (Roche) and 1 mM PMSF (Sigma-Aldrich) ...