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Citations for Roche :
551 - 600 of 10000+ citations for Glutathione S Transferase GST Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... 5 mM MgCl2, 1 mM EDTA, 1 mM EGTA, 1 mM DTT, 1 mM PMSF, 3 mM ATP, 10% sucrose and Roche protease inhibitors). For sS1 constructs ...
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bioRxiv - Biochemistry 2024Quote: ... 20 mM MgCl2, 1 mM EDTA, 1 mM EGTA, 10% sucrose, 3 mM ATP, 1 mM DTT, 1 mM PMSF and Roche Protease Inhibitors), 1 ml per dish ...
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bioRxiv - Cell Biology 2023Quote: ... 150 mM NaCl, 1 mM EDTA, 1mM EGTA, 1% Triton X-100, 0.4% SDS, 1% NP-40, 1× Roche protease inhibitor cocktail), one time with wash buffer B (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2019Quote: ... 1 mM PMSF with 1 X protease inhibitors (Roche) and incubated with 5-10 μg of purified MBP-tagged anillin or GST-tagged importin-β protein on beads at 4°C overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 X protease and 1 X phosphatase inhibitors (Roche), 10 % v/v glycerol) ...
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bioRxiv - Molecular Biology 2022Quote: ... HA tag (Roche 11666606001, 1:1,000 or 1:3,000), His tag (Abcam ab18184 ...
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bioRxiv - Neuroscience 2020Quote: ... 1 mg.mL−1 SDS) with Complete protease inhibitors (Roche). Equal amounts of protein (BCA protein assay ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 1 mg ml-1 collagenase/dispase (Roche) and 0.1 mg ml-1 DNase I (Roche ...
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bioRxiv - Plant Biology 2022Quote: ... 1 mM DTT and 1 × protease inhibitor cocktail (Roche)) ...
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bioRxiv - Cancer Biology 2023Quote: ... CD68 (clone KP-1 at 1:20, Ventana- Roche), FOLR2 (OTI4G6 at 1:200 ...
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bioRxiv - Immunology 2023Quote: ... supplemented with collagenase D (1 mg ml−1, Roche) and DNase I (0.25 mg ml−1 ...
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bioRxiv - Genomics 2019Quote: ... using the DNA 1000 kit and quantified by qPCR using the KAPA Library quantification kit (Roche, ref. KK4824). The library was sequenced on one lane of Hiseq2500 in single read 100nt mode using the clustering and SBS v3 kit following the manufacturer’s instructions.
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bioRxiv - Bioengineering 2021Quote: ... Microcarriers were subjected to total RNA extraction using a commercially available kit (High Pure kit, Roche, Indianapolis, IN). Total RNA was then used to make complementary DNA (cDNA ...
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bioRxiv - Cancer Biology 2021Quote: ... The detection kit for the antibodies is the UltraView DAB detection Kit (Ventana Medical Systems Inc./ Roche Diagnostic). A counter-staining of the nuclei was used for 12 minutes by Hematoxylin ...
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bioRxiv - Epidemiology 2019Quote: ... An accurate library quantification was determined using the Library Quantification Kit – Illumina/Universal Kit (KAPA Biosystems, Wilmington, MA). The amplicon pool was sequenced on the Illumina MiSeq generating paired end 300 bp reads ...
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bioRxiv - Cell Biology 2021Quote: ... RNA sequencing libraries were prepared using a stand specific kit RNA-seq kit with ribosome depletion (KAPA Biosystems) and NEBNext Adapters and Multiplex Oligos for Illumina ...
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bioRxiv - Physiology 2024Quote: ... TUNEL staining was performed using a commercial kit (in situ Cell Death Detection Kit, TMR red – Roche, Switzerland). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... DRGs were then triturated in a 1:1 mixture of 1 mg/mL trypsin inhibitor (Roche) and bovine serum albumin (BioPharm Laboratories) ...
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bioRxiv - Molecular Biology 2020Quote: ... genes were determined in 10 μL reaction mixtures of 96 wells clear Light Cycler 480 Multi well plate (#05102413001, Roche, USA). Triplicate 10 μL reaction mixtures were prepared that contained 4 μL either of 10 ng cDNA for gene of interest ...
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bioRxiv - Plant Biology 2021Quote: ... Amplification involved cDNA (2 μl, 5 ng/μl) in optical 384-well plates (Roche Light Cycler 480; Roche Diagnostics, Mannheim, Germany) with SYBR Green I dye and gene-specific primers (Supplemental Table S8) ...
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: Cells from one confluent well of a six-well plate were lysed in RIPA buffer plus c0mplete EDTA-free protease inhibitor tablets (Roche 11873580001) and spun for 10 minutes at 21,000xg at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were harvested from frozen plates by scraping into RIPA buffer containing cOmplete Mini EDTA-free Protease Inhibitor Cocktail (Roche 11836170001) and PhosSTOP Phosphatase Inhibitor Cocktail Tablets (Roche 04906845001 ...
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bioRxiv - Cancer Biology 2020Quote: Real-time measurements of cell migration and invasion were performed using the CIM-Plate 16 module of an xCELLigence System RTCA DP real-time cell analyzer (Roche, UK). For invasion measurement ...
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Interdependent Iron and Phosphorus Availability Controls Photosynthesis Through Retrograde SignalingbioRxiv - Plant Biology 2021Quote: ... Real-time quantitative reverse-transcription PCR (qRT-PCR) was performed as described in33 using 384-well plates with a LightCycler 480 Real-Time PCR System (Roche diagnostics). The Ubiquitin 10 mRNA (UBQ10 ...
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bioRxiv - Biophysics 2022Quote: ... A total of 24 μL of the qPCR mastermix was aliquotted in each well of a 96-well lightcycler plate (Roche 96) and 1 μL of RT reaction was aliquoted in each well ...
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bioRxiv - Microbiology 2021Quote: ... in 6 well plates were transfected with HSIV-vif plasmids using Fugene 6 or X-tremeGENE 9 DNA transfection reagent (Roche/Sigma). At 48 hour post-transfection ...
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bioRxiv - Cancer Biology 2020Quote: ... Individual sample mRNA levels were analysed in triplicate in a 96-well plate using an LC480 light cycler instrument (Roche Diagnostics). Gene expression levels were normalised against 18S.
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bioRxiv - Microbiology 2019Quote: ... Each reaction contained a 5μl of a 1 in 10 dilution of genomic DNA and was carried out in quadruplicate in a volume of 15 μl in a 384-well LightCycler® 480 PCR plates (Roche), sealed with LightCycler® 480 sealing foil (Roche) ...
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bioRxiv - Cell Biology 2020Quote: ... All reactions were performed in technical triplicate using 384-well plates on the LightCycler 480 System using the thermal cycling conditions as per the manufacturer’s instructions (Roche Diagnostics GmbH). The 2^DDCt method was used to calculate relative changes in gene expression and normalization to the arithmetic mean of both B2M and TBP.
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bioRxiv - Genetics 2021Quote: ... and the internal control vector TK (25 ng per well for 48-well plate) were co-transfected into the cells by using the X-tremeGene HP DNA transfection reagent (ROCHE, 6366236001). The transfected HEK293T cells were harvested in 65 μL passive lysis buffer (Promega ...
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bioRxiv - Cancer Biology 2021Quote: 1 μl sample from Denaturation was transferred to the qPCR plate well containing the qPCR Mix (Nuclease-free water + KAPA SYBR FAST qPCR Master Mix (KAPA Biosystems) + cDNA Primers (from Visium Reagent kit)) ...
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bioRxiv - Molecular Biology 2022Quote: ... Reactions were loaded into 96-well plates and were performed at 63 °C during 35 min using a LightCycler 96 Real-Time System (LC96) (Roche Diagnostics). One melting cycle was performed at 0.1 °C/s from 63 °C up to 97 °C for validation of the specificity of the amplified products ...
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bioRxiv - Microbiology 2022Quote: ... incubated overnight at 32°C and replica-plated on a YES agar plate containing 100 mg/L hygromycin B (Roche, #10843555001). The hygromycin plates were incubated for 1-2 days at 32°C until colonies had formed ...
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Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeatsbioRxiv - Molecular Biology 2023Quote: ... each well of cells from 24-well plates was lysed with 120 μL of RIPA buffer with protease inhibitor (Roche, 11836153001). All protein lysates were denatured with 12% β-mercapto-ethanol in 6x SDS-loading dye ...
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bioRxiv - Genomics 2023Quote: The 19.5 μl eluate was placed in one microwell of a 96-well plate preloaded with a PCR reaction mixture containing 25 μl of 2x KAPA HiFi HotStart Uracil+ ReadyMix (Roche, KK2801), 1.5 μl of 25 μM N5XX primer (ordered from IDT with the sequence shown in Supplementary Fig ...
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bioRxiv - Cell Biology 2023Quote: ... Transfection was performed on six-well plates or confocal dishes for different purposes with plasmids using X-tremeGENE HP DNA Transfection Reagent (Roche, 6366236001) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... using Kapa SYBR Fast Universal Kit (Roche) with an annealing temperature of 60°C and with primers listed in Supplementary Table S1 ...
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bioRxiv - Developmental Biology 2021Quote: ... using Library Quantification Kit (KK4854, Kapa Biosystems), and multiplexed by pooling equimolar amounts of each library prior to sequencing ...
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bioRxiv - Plant Biology 2022Quote: ... using SYBR Fast Universal kit (Kapa Biosystems). The average threshold cycle (Ct ...
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bioRxiv - Molecular Biology 2020Quote: ... or Kapa Library Quantification kit (Kapa Biosystems). One hundred cycle paired end sequencing was performed on an Illumina HiSeq 4000 (single copy strains ...
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bioRxiv - Pathology 2020Quote: ... DAB Map detection kit (Roche Diagnostics KK) was used for visualization ...
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bioRxiv - Genomics 2020Quote: ... using a nick translation kit (Roche Diagnostics).
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bioRxiv - Cell Biology 2021Quote: ... using KAPA Hyperprep mRNA Library Kit (Roche) and Illumina adapters (New England Biolabs ...
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bioRxiv - Cancer Biology 2020Quote: ... and Kapa Library Quantification Kit (Kapa Biosystems). Finally ...
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bioRxiv - Plant Biology 2021Quote: ... using KAPA SYBR FAST qPCR Kits (Roche) and the gene-specific primer sets shown in Supplementary Table ## ...
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Interdependent Iron and Phosphorus Availability Controls Photosynthesis Through Retrograde SignalingbioRxiv - Plant Biology 2021Quote: ... and the KAPA Library Quantification Kit (Roche). Pooled libraries were then sequenced using the NextSeq 500 System at the Stanford Functional Genomics Facility (Stanford ...
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bioRxiv - Genomics 2021Quote: ... Transcriptor High Fidelity cDNA Synthesis Kit (Roche) was used for cDNA synthesis with OligodT primers ...
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bioRxiv - Evolutionary Biology 2022Quote: ... KAPA SYBR Fast qPCR Kit (Roche, USA), Tn5-1789F and Tn5-1879R primer sets were used for quantification ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cytotoxicity detection kits were purchased from Roche Diagnostics Ltd (West Sussex ...
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bioRxiv - Immunology 2022Quote: Cytotoxicity Detection LDH Kit (Roche, Indianapolis, USA) was used on cell supernatants according to manufacturer’s instructions ...