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Citations for Roche :
551 - 600 of 608 citations for Dioctanoyl phosphatidic acid sodium salt since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... DNA extraction were carried out using MagNA Pure LC total Nucleic acid isolation kit on the automated MagNA Pure LC2.0 platform (Roche) from two to five colonies picked up from fresh cultured plates.
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bioRxiv - Microbiology 2024Quote: ... the nucleic acid pellet was resuspended in 38μL of DNase buffer and 2μL of 10U/μL DNase I (Roche, #04716728001), gently mixed ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... titrated to pH 8.1 with phosphoric acid) with protease and phosphatase inhibitors added (Roche, CO-RO and PHOSS-RO) and then sonicated ...
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bioRxiv - Microbiology 2024Quote: ... Viral RNA was purified with High Pure Viral Nucleic Acid Kit according to the manufacturer’s instruction (ROCHE, Mannheim, Germany). Viral RNA was amplified by LightMix® Modular Sarbecovirus E-gene (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... 1% Triton X-100, 150mM NaCl, 50mM Tris pH 7.5, 10mM MgCl2, 5mM NaF, 1mM sodium orthovanadate, and Roche EDTA-free protease inhibitor cocktail). Unstimulated control cells were lysed in solution by adding an equal volume of 2x cold lysis buffer for a final volume of 500 μL ...
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bioRxiv - Biochemistry 2023Quote: ... and were resuspended in 30 mL lysis buffer 1 (50 mM sodium phosphate, 300 mM NaCl, pH 8) containing 2 mL of EDTA-free protease inhibitor mixture (Roche Applied Science, Penzberg, Germany). Cells were disrupted by passing the cell suspension three times through a Constant Cell disruption system (TS benchtop ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was diluted 5-fold with load dilution buffer (solubilization buffer supplemented with 1% (w/v) sodium cholate) and loaded on a Ni-NTA affinity column (Roche cOmplete Ni-NTA resin) equilibrated with 10 CVs load dilution buffer ...
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bioRxiv - Cell Biology 2020Quote: ... L6 cells and cardiomyocytes by incubating them in their respective media containing 2% (w/v) fatty acid-free bovine serum albumin (FAF-BSA; Roche) and 0.4 mM sodium palmitate (Sigma ...
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bioRxiv - Genetics 2021Quote: ... For whole-genome sequencing DNA was extracted using the MagNA Pure LC Total Nucleic Acid Isolation Kit (Roche Diagnostics GmbH).
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bioRxiv - Molecular Biology 2021Quote: ... lysate supernatant from cells expressing N-terminal 6-His-tagged proteins were incubated with nickel-nitrilotriacetic acid (Ni-NTA) cOmplete His-tag purification resin (Roche) for 1 h at 4° C ...
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bioRxiv - Microbiology 2020Quote: ... Viral transport media was added to each pool at a 1:1 ratio for nucleic acid extraction performed on the Roche MagNA Pure 24 platform using the MagNA Pure 24 Total NA Isolation kit (Roche). Elution volume was set to 50 ul to concentrate viral RNA ...
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bioRxiv - Microbiology 2021Quote: ... was extracted from inactivated samples (200 μL) using the MagNA Pure Compact instrument and MagNA Pure Compact Nucleic Acid Isolation Kit I (Roche Molecular Systems Inc ...
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bioRxiv - Cell Biology 2020Quote: ... MEFs were seeded subconfluently o/n (unless indicated otherwise) onto coverslips acid-washed and coated with human fibronectin (25 μg/ml, #11051407001, Roche), as described (Dimchev and Rottner ...
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bioRxiv - Neuroscience 2021Quote: ... Two AfeI restriction sites were sequentially added by two rounds of PCR-amplification on each side of individual IDR (see Fig. 1b and S1b for amino acid position) using KAPA HiFi polymerase (Roche). The PCR reaction was DpnI digested (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from 200 μl of another aliquot of lung homogenate supernatant using the MagNA Pure Total Nucleic Acid Isolation protocol and reagents on a MagNA Pure LC 2.0 instrument (Roche Diagnostics). Prior to extraction ...
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bioRxiv - Microbiology 2020Quote: ... whole specimens were processed into head/thorax homogenates for RNA extraction with the High Pure Viral Nucleic Acid Kit (Roche), according to manufacturer’s instructions (Moreira et al. ...
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bioRxiv - Immunology 2020Quote: Genomic DNA was extracted from homogenized tissue of adenovirus vectored vaccine inoculated mice by High Pure Viral Nucleic Acid Kit (Roche). DNA fragments of Sad23L and Ad49L vectors were amplified by nested PCR with primers specific to adenoviral hexon sequences (Table S4 ...
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bioRxiv - Immunology 2023Quote: ... 1 mM EDTA) and then detected with 2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium (ABTS) substrate (Roche Diagnostics) diluted at 1:1000 in H2O2 ...
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bioRxiv - Immunology 2023Quote: ... 1 mM EDTA) and then detected with 2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium (ABTS) substrate (Roche Diagnostics) diluted at 1:1000 in H2O2 ...
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bioRxiv - Microbiology 2023Quote: ... and nucleic acid extracted using the MagNA 96 instrument with DNA/Viral NA small volume kits (Roche, Laval, Quebec, Canada). Ophidiomyces ophidiicola was not detected in these samples by qPCR (Allender et al ...
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bioRxiv - Microbiology 2022Quote: ... Nucleic acids were spotted (for dot blotting) or electro-transferred (for Northern blot) to positively-charged nylon membranes (Roche Diagnostics), and hybridized with DIG-labeled riboprobes as previously described (22).
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bioRxiv - Cell Biology 2023Quote: ... The membrane was blocked with freshly prepared blocking solution (100 mM maleic acid, 150 mM NaCl, pH 7.5, 1% (wt/vol) blocking reagent (Roche, 11096176001)) for 30 min ...
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bioRxiv - Genomics 2022Quote: Cells were lysed in RIPA buffer (Beyotime, China) containing protease inhibitors (Complete Mini Ethylene Diamine Tetraacetic Acid-Free Protease Inhibitor Cocktail, Roche). The protein lysates were centrifuged ...
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bioRxiv - Microbiology 2022Quote: ... lacking the predicted signal peptide (amino acids 1-29) was amplified from genomic DNA (isolated using the High Pure Template kit, Roche) by PCR using the Phusion Hot start II High Fidelity DNA Polymerase (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Nucleic acids were spotted (for dot-blot) or electro transferred (for northern-blot) to positively-charged nylon membranes (Roche Diagnostics), and hybridized with DIG-labeled full-length riboprobes as previously described [8 ...
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bioRxiv - Biochemistry 2023Quote: ... uridine 5’-diphospho-N-acetyl-glucosamine (UDP-GlcNAc) and cytidine-5’-monophospho-N-acetylneuraminic acid (CMP-Neu5Ac) were obtained from Roche Diagnostics [UDP-Gal ...
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bioRxiv - Cell Biology 2023Quote: ... embryos were incubated for 1 hour in TBST and then in Blocking Buffer (2% blocking reagent in maleic acid buffer pH 7.5 (Roche, #11096176001) and 10% sheep serum (Sigma Aldrich ...
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bioRxiv - Zoology 2024Quote: ... nucleic acids were extracted from 200 µL of EDTA-blood using the Magnapure 96 robotic extraction machine (Roche, Basel, Switzerland) in combination with the Magnapure 96 DNA and viral NA small volume kit ...
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bioRxiv - Bioengineering 2024Quote: ... sulfanilamide (Reagent I) and N-(1-Naphthyl)-Ethylenediamine in hydrochloric acid (Reagent II) from the “Nitrite/Nitrate Colorimetric Test” (Roche) kit (#11746081001 ...
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bioRxiv - Developmental Biology 2022Quote: ... 100 µl 10% SDS, 500 µl 20% N-Lauroyl sarsosine sodium, 2 tablets of cOmplete ULTRA Protease Inhibitor Cocktail [Roche Cat.# 05892970001] in 10ml FA buffer). For each IP experiment ...
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bioRxiv - Microbiology 2020Quote: ... DNA extractions performed at the MPI-SHH substituted the column apparatus from the High Pure Viral Nucleic Acid Large Volume Kit (Roche, Switzerland) in place of the custom assembled Zymo-reservoirs coupled to MinElute (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: ... three-to-four 5μm FFPE samples sections were taken for RNA extraction using commercial FFPE nucleic acid isolation kit (Roche Molecular Diagnostics). RNA was quantified using Nanodrop and analyzed for fragment distribution using a bioanalyzer (Agilent) ...
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bioRxiv - Molecular Biology 2022Quote: ... uric acid and phosphate) were analyzed and quantified by the Service de Chimie Clinique du CHUV using a Cobas 8000 (Roche Diagnostics).
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bioRxiv - Biochemistry 2021Quote: ... the amino acid Thr at position 206 was mutated to Trp using KAPA HiFi HotStart ReadyMix PCR Kit (Kapa Biosystems, USA) together with the designed complementary primers (5’- CCTATCTGATTCATGAGCACATGGTTATTTGGGATCGCATTGAAAAC-3’ and 5’- GTTTTCAATGCGATCCCAAATAACCATGTGCTCATGAATCAGATAGG-3’ ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mM β-mercaptoethanol and 10% glycerol) plus 20 mM imidazole and supplemented with ethylenediaminetetraacetic acid (EDTA)-free protease inhibitor cocktail tablets (Roche Diagnostics). Cells were lysed using a cell disrupter (Constant Systems ...
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bioRxiv - Developmental Biology 2023Quote: ... The samples were then washed with 0.1% PBST and incubated in 1% blocking buffer (1% blocking reagent [Roche] in Maleic acid) for 1 h ...
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bioRxiv - Immunology 2022Quote: Viral RNA was extracted using the MagNA Pure 96 DNA and Viral Nucleic Acid kit on the MagNA Pure 96 system (Roche Diagnostics) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... resuspended at 10 mg/mL in AC/BT buffer (1.5M aminocaproic acid and 75mM Bis-Tris/HCl, pH 7.0, supplemented with Complete Mini Protease Inhibitor [Roche Diagnostics, Stockholm, Sweden]), and kept frozen at -80°C until analysis ...
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bioRxiv - Plant Biology 2022Quote: ... The products then transferred from the gel to Hybond N+ Nylon membrane (Amersham Biosciences) and detected using DIG Nucleic Acid Detection Kit (Roche Diagnostics) according to manufacturer’s protocol.
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bioRxiv - Plant Biology 2022Quote: ... The products were then transferred from the gel to the Hybond N+ Nylon membrane (Amersham Biosciences) and detected using DIG Nucleic Acid Detection Kit (Roche Diagnostics), according to the manufacturer’s protocol.
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bioRxiv - Biochemistry 2023Quote: ... 0.6 mL of incubation buffer (citric acid 100 mM and NaCl 250 mM pH 5.5 with inhibitor cocktail (Roche, Rotkreuz, Switzerland) dilution according to the instructions of the manufacturer) ...
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bioRxiv - Bioengineering 2024Quote: ... We purified the AAV genomes from the envelope-AAV samples using the High Pure Viral Nucleic Acid Kit (Roche, Indianapolis, IN). For all AAV-based vectors ...
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bioRxiv - Biophysics 2024Quote: Cells were lysed by sonication in lysis buffer (20 mM Tris, 150 mM NaCl pH 8, with Roche Ethylenediaminetetraacetic Acid (EDTA)-free protease inhibitor cocktail (Sigma-Aldrich Chemie GmbH)) ...
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bioRxiv - Developmental Biology 2022Quote: ... The samples were then washed with 0.1% PBST and incubated in 1% blocking buffer (1% blocking reagent [Roche, 11175041910] in Maleic acid) for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... Total nucleic acid was extracted from the lysate using the Roche MagNA Pure Compact Nucleic Acid Isolation Kit I and the MagNA Pure Compact instrument (Roche, Indianapolis, Indiana). Dual-indexed sequencing libraries were prepared from the lysates using Nextera XT DNA Library Preparation Kit (Illumina FC-131-1096) ...
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bioRxiv - Microbiology 2024Quote: ... Viral RNA isolation was performed using the MagNA Pure LC system (Total nucleic acid isolation kit, Roche Molecular System, Laval, QC, Canada) or the EZ2 Connect system (EZ1&2 Virus Mini Kit v2.0 ...
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bioRxiv - Cell Biology 2024Quote: ... mRNA levels were determined using gene-specific primers by SYBR green nucleic acid labeling (Selleckchem PCR Mastermix, #B21202) in a Lightcycler 480 (Roche, Indianapolis, IN). Fold change was calculated using the delta delta C(t ...
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bioRxiv - Microbiology 2022Quote: ... Samples were transferred to a high pure extender assembly from the High Pure Viral Nucleic Acid Large Volume kit (Roche, Mannheim, Germany) and centrifuged for 8 min with 1,500 rpm at room temperature ...
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bioRxiv - Synthetic Biology 2022Quote: ... 140 mM NaCl, 1mM ethylenediaminetetraacetic acid, 1% Triton X-100, 0.1% Na-Deoxycholate, 1 mM phenylmethylsulfonyl fluoride, 200 µL Roche cOmplete protease inhibitors). 0.5 mm diameter glass beads were added to 1 mm below the meniscus ...
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bioRxiv - Immunology 2024Quote: ... dissolved in Milli-Q water at the manufacturer specified concentration and supplemented with 0.1% fatty acid free bovine serum albumin (Roche Diagnostics, Mannheim, Germany), 1X Glutamax ...