Labshake search
Citations for Roche :
551 - 600 of 3871 citations for 4F2 Cell Surface Antigen Light Chain SLC7A5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... Amplification involved cDNA (2 μl, 5 ng/μl) in optical 384-well plates (Roche Light Cycler 480; Roche Diagnostics, Mannheim, Germany) with SYBR Green I dye and gene-specific primers (Supplemental Table S8) ...
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bioRxiv - Plant Biology 2020Quote: ... and 6.8 μL ddH2O were used and the RT-qPCR was performed using a Light Cycler 96 Real-Time PCR Machine (Roche, Germany). Expression levels were calculated using the 2-ΔCT equation (Kilambi et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... for 40 cycles at 95°C for 5 s and 60°C for 30 s on a Light Cycler 480 Real-Time PCR System (Roche, Switzerland). All primer sequences used in this study are listed in Supplementary materials (Supplementary Table S1).
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bioRxiv - Cancer Biology 2020Quote: ... Individual sample mRNA levels were analysed in triplicate in a 96-well plate using an LC480 light cycler instrument (Roche Diagnostics). Gene expression levels were normalised against 18S.
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bioRxiv - Physiology 2021Quote: ... Gene expression of the target genes fshb and lhb was quantified by qPCR using the Light Cycler 96 (Roche, Basel, Switzerland) thermocycler and SYBR Green I master (Roche ...
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bioRxiv - Plant Biology 2021Quote: ... and NADP-ME3 (Zm00004b015828) were quantified via qRT-PCR using a Roche Light Cycler 480 II using Roche SYBR green I (Roche 04707516001). The delta cT method was used to quantify relative gene expression of PEPCK1 and NADP-ME3 over time ...
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bioRxiv - Microbiology 2022Quote: ... amplification was performed in a 20-μl reaction mixture containing 1× Light Cycler Fast Start DNA master hybridization probes (Roche Diagnostics), 4 mM MgCl2 ...
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bioRxiv - Cell Biology 2022Quote: ... Real-time PCR was carried out with the Light Cycler Fast Start DNA Master SYBR Green Kit (Roche Applied Science, Germany) using LightCycler (Roche Applied Science ...
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bioRxiv - Microbiology 2022Quote: ... The total HIV-1 DNA was amplified by quantitative real-time PCR using the Light Cycler instrument (Roche Diagnostics, Meylan, France). Amplification was performed in a 20 μl reaction mixture containing 1× Light Cycler Fast Start DNA master hybridization probes (Roche Diagnostics) ...
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bioRxiv - Microbiology 2022Quote: ... Amplification was performed in a 20 μl reaction mixture containing 1× Light Cycler Fast Start DNA master hybridization probes (Roche Diagnostics), 3 mM MgCl2 ...
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bioRxiv - Cancer Biology 2023Quote: ... along with a panel of 3 reference genes (PUM1, RPL13A, ACTB) was performed using TaqMan qPCR chemistry on the Light Cycler 480 II (Roche Diagnostics). According to previously established methods 7 ...
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bioRxiv - Genetics 2023Quote: ... We performed copy number qPCR using genomic DNA from three humanized and three wild type individuals using Light Cycler 480 SYBR Green I Master Mix (Roche #04707516001). The biological replicates for each individual were run in triplicate and error bars show the standard deviation from these technical replicates ...
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bioRxiv - Microbiology 2023Quote: ... qPCR standard curves were determined using linearized plasmids as DNA templates and run on a Light Cycler 480 system (Roche Diagnostics) in duplicates ...
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bioRxiv - Molecular Biology 2023Quote: ... The same proportion of cDNA products of each sample was used as template for the quantitative RT-PCR reaction with Light Cycler 480 SYBR Green I Master Mix (Roche, 04707516001). The Ct value was used to represent the absolute amount of EU-RNAs in each sample ...
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bioRxiv - Neuroscience 2024Quote: ... titer was determined at a concentration of 1.1013 gcp/ml by quantitative real-time PCR using the Light Cycler 480 SYBR green master mix (Roche, cat # 04887352001) with primers specific for the AAV2 ITRs (fwd 50-GGAACCCCTAGTGATG GAGTT-3’ ...
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bioRxiv - Cancer Biology 2021Quote: ... size and concentration of cDNA libraries were checked using the Standard Sensitivity NGS kit Fragment Analyzer and qPCR (ROCHE Light Cycler 480). Libraries were sequenced using an Illumina Hiseq 2500 sequencer as single-end 125 base reads ...
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bioRxiv - Genomics 2019Quote: ... Adapters-ligated cDNAs were then amplified by PCR and libraries were validated on Fragment Analyzer with a Standard Sensitivity NGS kit (Advanced Analytical Technologies, Inc) and quantified by qPCR with a Light Cycler 480 thermal cycler (Roche Molecular diagnostics).
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bioRxiv - Plant Biology 2021Quote: ... cDNA synthesis and qPCR was performed as described previously (Pal et al., 2017) on Roche Light Cycler 96 (Roche Diagnostics, Mannheim, Germany). Zea mays elongation factor (ZmEF α1 ...
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bioRxiv - Microbiology 2021Quote: ... A single sample (one mouse) was assessed on each array plate on a Roche Light Cycler 480 II real time PCR thermal cycler (Roche, Basel, Switzerland). All data were analyzed with Qiagen’s Gene Globe online analysis application ...
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bioRxiv - Immunology 2020Quote: ... CD206 and CD64 was performed by Fast Start DNA Masterplus SYBR Green I kit on the Light Cycler 480 (Roche, Applied Science). Relative RNA expression levels were normalized to the geometric mean of two reference genes RPL37A and ACTB ...
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bioRxiv - Genetics 2022Quote: ... Quantitative PCR was performed in 4 replicates in final volume of 10 µL using Light SYBR Green PCR Master Mix (Roche, Applied Science) on a LightCycler 480 Real-Time PCR System (Roche ...
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bioRxiv - Molecular Biology 2023Quote: We designed a multiplex quantitative PCR (qPCR) with four parallel assays to be run on the Roche 480 light cycler (Roche, Basel, Switzerland). Compatible fluorescent dyes (FAM ...
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bioRxiv - Developmental Biology 2023Quote: ... Average library size and quality control were performed using a Fragment Analyzer (High Sensitivity NGS kit) and qPCR (Roche Light Cycler 480). CUT&RUN libraries were sequenced on a NovaSeq 6000 (Illumina ...
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bioRxiv - Plant Biology 2024Quote: ... RT-qPCR was performed using gene-specific primers (Supplemental Table S3) using Promega Go Taq® qPCR Master Mix on a Light Cycler 480 II® (Roche) thermocycler ...
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bioRxiv - Plant Biology 2024Quote: ... Templates were amplified in KOD SYBR qRT-PCR Mix (TOYOBO) using a Light Cycler Nano Real-time PCR Detection System (Roche Applied Science). The PCR was performed according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... size and concentration of cDNA libraries were checked using the High Sensitivity NGS kit Fragment Analyzer and qPCR (ROCHE Light Cycler 480). Libraries were sequenced using an Illumina Novaseq 6000 sequencer as single-end 100 base reads ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were incubated overnight at 4 °C with rat anti-HA antibody (1:500 - Roche) and then with secondary goat anti-rat IgG antibody coupled to Alexa 568 (Invitrogen/Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated for 10 min at 37°C with anti-HA antibody (3F10; Roche) (1:3000 ...
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bioRxiv - Microbiology 2021Quote: ... cells were labeled beforehand for 30 minutes with an anti HA antibody (3F10, Roche 11867423001) diluted at 1:200 in PBA ...
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bioRxiv - Cell Biology 2019Quote: ... One μl of each eluted supernatant-DNA sample was added to a reaction mixture containing 2X Light Cycler480 SYBR Green I master mix (Roche Applied Science, Germany) and 0.25 μM of forward and reverse primers (ALU-115 and ALU-247 ...
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bioRxiv - Genetics 2021Quote: ... The RT-PCR reaction was carried out in a Light Cycler 480 fluorescence quantitative meter (Fritz Hoffmann-La Roche Co. Ltd., Basel, Switzerland).
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bioRxiv - Cell Biology 2021Quote: ... using 2 μL of the diluted cDNAs and the KAPA SYBR ® FAST qPCR Kit Optimized for Light Cycler ® 480 (KAPA biosystems). Differences between samples and controls were calculated based on the 2−ΔCT method ...
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bioRxiv - Cell Biology 2020Quote: ... 2ul of DNA (20 ng of DNA) was added at 10ul of a reaction buffer (Light Cycler Fast Start DNA Master SYBR Green Kit, Roche Diagnostics, Basilea, SWI) and 1% SDS ...
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bioRxiv - Plant Biology 2020Quote: ... Real-time quantitative PCR assays were performed using the KOD SYBR® qPCR Mix (TOYOBO) on the Light Cycler 96 system (Roche Diagnostics, Germany). ΔCt method was used for estimating transcript abundance ...
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bioRxiv - Developmental Biology 2022Quote: ... QPCR was performed using the LightCycler® FastStart DNA Master PLUS SYBR Green I kit and a light cycler 2.0 (Roche Life Science, France) as previously reported 7
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bioRxiv - Pathology 2023Quote: ... The RT-qPCR reactions were performed as recommended by the kit protocol and run on a Roche Light Cycler (Roche, Indianapolis, IN, USA). The limit for cycle of quantification was defined at less than 29 ...
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bioRxiv - Genomics 2023Quote: ... Bank validation was done with the quantification of the complementary DNA with the Standard Sensitivity NGS kit on Fragment Analyzer and with qPCR (ROCHE Light Cycler 480). The sequencing was done on NovaSeq 6000 (Illumina ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates (0.5 mg) were incubated with anti-HA antibody-coupled agarose beads (Roche, Basel, Switzerland) for 16 h at 4 °C ...
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bioRxiv - Biophysics 2021Quote: ... Monoclonal cell lines were identified by immunocytochemistry using a rat anti-HA antibody (Roche Applied Science). HEK293 cells stably overexpressing rat sACt were as described (58).
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bioRxiv - Microbiology 2020Quote: ... qRT-PCR reactions (10 µl) were set up in Light cycler 480 plates using the LightCycler 480 probe master mix (Roche, Welwyn Garden City UK) according to the manufacturer’s instructions (Tables 2) ...
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bioRxiv - Biophysics 2022Quote: ... Antibodies used: Mouse anti-GFP primary antibody (Roche) at 1:3,000 dilution ...
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bioRxiv - Microbiology 2021Quote: ... Samples were considered not infected when no Cp value was calculated using Absolute quantification/2nd derivative Max analysis on the Light Cycler® 480 (Roche, Software release 1.5.1.62 SPS).
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bioRxiv - Cell Biology 2020Quote: ... the cells were incubated with the anti-HA high affinity antibody (3F10) (1:500 dilution, Roche – cat. 11867423001), followed by incubation with an anti-rat secondary antibody conjugated to Alexa Fluor 488 (1:2000 dilution ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then incubated with primary antibodies used at the following dilutions: mouse-anti-HA (Roche, 1:300), rabbit-anti-γ-H2A.X (Cell signaling ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were incubated in PEMBALG containing monoclonal anti-HA antibody produced in rat (1:1000 dilution, Roche) for one hour ...
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bioRxiv - Cell Biology 2022Quote: ... cell lysate was incubated with agarose beads conjugated with either mouse IgG or mouse anti-GFP antibodies (Roche) for 3h at 4°c under gentle agitation ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then incubated with 40 µL of primary antibody (1:1,000 rat anti-HA mAb 3F10, Roche) at 4°C overnight in a solution containing 3% BSA in PBS ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were then stained for HMGXB4-HA and HMGXB4SUMO -HA using a HA tag antibody (Roche Applied Science) and Fibrillin with Anti-Fibrillin 1 antibody (abcam ...
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bioRxiv - Microbiology 2020Quote: ... IFAs were carried out on methanol-fixed cells using primary antibodies mouse mAb α-GFP (Roche Diagnostics #11814460001), 1:100 and rabbit α-PfHP1 12 ...
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bioRxiv - Immunology 2023Quote: ... sorted cells were washed with PBS and resuspended in Antibody Buffer (1X eBioscience Perm/Wash Buffer, 1X Roche cOmplete EDTA-free Protease Inhibitor ...