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Citations for Roche :
551 - 600 of 1900 citations for 2 6 Amino 9H purin 9 yl ethanol d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... the embryos are rinsed in TBS/0.1% Tween-20 (TBST) then blocked for 2 h in 2% blocking solution (Roche) and incubated O/N in the same solution containing 1:2,500 anti-digoxigenin antibody (Roche) ...
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bioRxiv - Plant Biology 2023Quote: ... in 100μl extraction buffer (Tris-HCl 50 mM pH 6.8, SDS 2%, DTT 2 mM and 1× protease inhibitors (Roche) and centrifuged for 5 min at 13,000g at 4OC ...
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bioRxiv - Immunology 2023Quote: ... cells were lysed in 100μL or 200μl of 2% SDS lysis buffer (2% SDS, 50mM Tris-HCl pH 7.5, 5mM EDTA, 15U/mL DnaseI (Roche), cOmplete mini EDTA-free protease inhibitor tablet (Roche ...
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bioRxiv - Microbiology 2021Quote: ... monocytes were harvested using ice-cold lysis buffer (1% Triton X-100, 2% SDS, 150 mM NaCl, 10 mM HEPES, 2 mM EDTA containing protease inhibitor cocktail - Roche). Cell lysates were heated at 100 °C for 5 min in the presence of Laemmli buffer (20% β-mercaptoethanol ...
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bioRxiv - Biochemistry 2021Quote: ... 2×106 cells HEK293T cells were transfected with 2 μg plasmid DNA using X-tremeGENE HP DNA Transfection Reagent (Roche) in 10-cm dishes ...
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bioRxiv - Immunology 2023Quote: ... dissociated from the coverslips in 8 M urea lysis buffer (100 mM NaCl, 25 mM Tris, 2% SDS, 0.1% tween 20, 2 mM EDTA, 0.2 mM PMSF, and 1x Roche cOmpleteProtease inhibitor). Lysates were treated with benzonase (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... The parasites were pelleted by centrifugation at 25,000 x g and resuspended in 5 × the pellet volume with hypotonic lysis buffer (1 mM HEPES-NaOH pH 7.4, 2 mM EGTA, 2 mM DTT with protease inhibitor cocktail; cOmplete™, EDTA-free, Roche). The parasite suspension was incubated for 10 min on ice and then lysed by approximately 30 passages through a 1mL syringe fitted with a 27G needle.
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bioRxiv - Plant Biology 2022Quote: ... 150 mM NaCl, 20 mM KCl, 2 mM MgCl2, 1% TX-100, 40U Ribolock ml-2 and protease inhibitor cocktail, Roche) followed by clearing at 17 000 × g for 10 min at +4C° ...
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bioRxiv - Immunology 2024Quote: ... Whole lymph nodes or chopped spleens were then transferred into 15 ml conical tubes containing 2 ml digestion buffer (RPMI containing 2% FBS, 0.1mg/ml DNAse I (Roche, #10104159001), 0.2 m/ml collagenase P (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were transfected with siRNAs or plasmid DNAs using Dharmafect 4 (Dharmacon) or Fugene 6 (Roche Applied Sciences) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Cells were seeded in 10 cm tissue-culture treated dishes and transfected with FuGENE 6® reagent (Roche) for 24 hr ...
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bioRxiv - Biochemistry 2022Quote: (His)6-GST-SNX15 MIT was bound to cOmplete His-Tag purification beads (5 mL, Roche, Germany, 2h) and washed with 2 L wash buffer ...
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bioRxiv - Neuroscience 2022Quote: ... hDAT and Stx1 constructs were transiently cotransfected into these cells (hDAT cells) using Fugene-6 (Roche Molecular Biochemicals) per the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... The mini-genes in the pSPL3b vector were transiently transfected using 6µl of FuGENE 6 Transfection Reagent (Roche) with 2 µg of vector ...
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bioRxiv - Evolutionary Biology 2020Quote: ... five pupae were homogenized in 100uL of homogenization buffer (125 mM Tris pH 6.8, 6% SDS, 2.5X Roche cOmplete protease inhibitor cocktail ...
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bioRxiv - Biochemistry 2024Quote: ... an additional 6-cycle PCR was carried out with the HiFi HotStart Ready Mix (Kapa Biosystems, Wilmington, MA) and primers that preserve the DNA sequence ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant containing chromatin fragments was then treated with 6-10 µg/g cells DNase-free RNase (Roche) at 37°C for 30 minutes and subsequently cleared by centrifugation at 30,000 rcf for 30 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Vectors were co-transfected with packaging-defective helper plasmids into 293T cells using Fugene 6 transfection reagent (Roche). Fibroblasts were plated at a density of 50,000 cells/well on 0.1% gelatin-coated 6-well plates and infected three times with a viral cocktail containing vectors expressing OCT4:SOX2:KLF4:cMYC in a 2:1:1:1 ratio in the presence of 6 µg/ml protamine sulfate (Sigma Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue pieces were then moved to a 6-well plate and incubated with Collagenase D (0.24U/mg, Roche) and DNase I (10U/µl ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 mM EDTA with 1x protease inhibitors (Roche). Protein concentrations were quantified with a BCA Kit (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: ... For cDNA synthesis 2 μg of DNaseI (Roche) treated total RNA was reverse transcribed using M-MuLV reverse transcriptase (Minotech) ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 µl 5x KAPA HiFi buffer (Kapa Biosystems), 0.3 µl 10 mM dNTPs (Kapa Biosystems) ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 mM 2-mercaptoethanol) with protease inhibitors (Roche) and 300 U/L benzonase (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... and/or DAPI (Roche: 10236276001, 2 μg/mL) before mounting to visualize barrel map ...
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bioRxiv - Biophysics 2020Quote: ... supplemented with 100 U/mL Il-2 (Roche). K562 cells were obtained from ATCC (CCL-243 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... containing 2 mg/ml Collagenase A (#10103586001, Roche), 2.4 U/ml Dispase II (#04942078001 ...
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bioRxiv - Biochemistry 2020Quote: ... 5 mM 2-Mercaptoethanol and protease inhibitor (Roche). The lysis proceeded by 3 passages in a French press cell at a pressure of 1500 psi ...
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bioRxiv - Cell Biology 2021Quote: ... containing 2 μg ml−1 collagenase A (Roche), 2.4 U ml−1 dispase I (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... and 2 μg/mL DNAse (11284932001, Roche Diagnostics), and incubated for at least 10 min on ice before sonication (10 cycles of 15 s with 30 s cooling at 8 microns amplitude ...
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bioRxiv - Molecular Biology 2020Quote: ... 2% BSA fraction V (Roche Diagnostics, Mannheim, Germany), 10 mM nicotinamide (Merck Millipore ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 mg/ml protease inhibitor cocktail (cOmplete, Roche)] on ice for 30 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... 2 tablets of the protease inhibitor cocktail (Roche) per 100 mL] ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 tablet of complete protease inhibitor cocktail (Roche) 2mM DTT ...
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... anti-PD-L1 (atezolizumab, Roche, 2 mg/kg), anti-TGF-β (mouse IgG1 clone 1D11 from BioXCell ...
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... anti-PD-L1 (atezolizumab, Roche, 2 mg/kg), or a combination of anti-PD-L1 (2 mg/kg ...
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bioRxiv - Cancer Biology 2020Quote: ... 20 µg/mL Liberase Blendzyme 2 (Roche, 05401020001) and 5 ml of Penicillin/Streptomycin (Gibco Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... and 100 U/ml of interleukin-2 (Roche) (complete IMDM) ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 mM EDTA with 1x protease inhibitors (Roche). 50 ug lysate was used for label free quantification at the Rockefeller University Proteomics Core Facility ...
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bioRxiv - Bioengineering 2021Quote: ... and 2 mg/ml DNase I (Roche, 11284932001) to obtain a single cell suspension.
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bioRxiv - Cell Biology 2020Quote: ... and 2 μg/ml collagenase A (Roche 10103586001). Tissue preparations were incubated at 37 °C in a water bath (in gently shaking and not in immersion ...
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bioRxiv - Immunology 2021Quote: ... then with Collagenase/Dispase (2 mg/mL) (Roche) and DNase I (2 mg/mL ...
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bioRxiv - Immunology 2021Quote: ... containing 2 mg ml-1 collagenase/ dispase (Roche) and 0.2 mg ml-1 DNase I (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 µl 5x KAPA HiFi buffer (Kapa Biosystems), 0.3 µl 10 mM dNTPs (Kapa Biosystems) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 tablets complete EDTA-free protease inhibitor (Roche)) followed by 5 min incubation at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 ml of diluted Liberase TH (Roche, 05401151001) was then added to the plate ...
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bioRxiv - Cell Biology 2023Quote: ... digested in 2 mg/ml Collagenase P (Roche) for 45 min and filtered through sieves with 70 and 40 µm pore size ...
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bioRxiv - Molecular Biology 2023Quote: ... and KAPA HiFi Uracil+ mastermix (Roche, KK2801/2) using the following protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... BSA fraction V (2% wt/vol) (Roche Diagnostics), 2-mercaptoethanol (50 µM) ...
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bioRxiv - Microbiology 2023Quote: ... 2 mg/ml lysozyme and protease inhibitors (Roche) per 1 L of culture ...
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bioRxiv - Neuroscience 2023Quote: ... 2% sodium dodecyl buffer (SDS)] containing protease (Roche) and phosphatase (Thermo Scientific ...