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Citations for Roche :
5801 - 5850 of 9754 citations for Monoisodecyl Phthalate 100 Ug Ml In Mtbe Unlabeled Mono 3 7 Dimethyl 1 Octyl Phthalate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... A single-cell solution was obtained by tissue digestion using 1mgml−1 collagenase D and 0.1mgml−1 DNase I (Roche) in HBSS (Lonza ...
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bioRxiv - Neuroscience 2020Quote: ... pH 7.5, 1 mM EGTA, 10% sucrose, 0.8 M NaCl containing sarkosyl (final 1%, w/v) and protease inhibitor (Roche). After ultracentrifugation at 135,000 × g for 20 min at 25 °C ...
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bioRxiv - Genetics 2019Quote: Cells were lysed with modified RIPA (50 mM Tris-HCL, 1% NP40, 0.25% Na-deoxycholate, 150 mM NaCl, and 1 mM EDTA; CompleteTM Protease Inhibitor Cocktail [Roche]), and protein concentrations were determined with the BCA-Kit (Pierce) ...
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bioRxiv - Molecular Biology 2019Quote: ... in a 1:1 volume ratio of Tris-buffered saline (pH 8.0) with added proteinase inhibitors (EDTA-free; Roche). Sample homogenization was confirmed by microscopic inspection ...
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bioRxiv - Cancer Biology 2019Quote: ... 0.1% SDS, 1% deoxycholic acid, 0.5 mM PMSF, 1 mM DTT, 0.1 mM sodium orthovanadate, and Roche protease inhibitors). Nuclear lysates were sonicated with a Branson 250 Sonifier (output 20% ...
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bioRxiv - Developmental Biology 2020Quote: ... Whole cells extract was isolated using RIPA buffer (NaCl 150mM – Tris HCL pH7,35 50mM – DOC 1% – N-P40 1% – H2O) supplemented with protease inhibitor (04 693 116 001, Roche). Isolated protein concentration was determined using Bradford assay (500-0006 ...
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bioRxiv - Molecular Biology 2021Quote: ... 1.5 mM MgCl2, 1 mM EDTA, 1 mM DTT, 0.1% NP-40, and a protease inhibitor cocktail tablet [Roche]). After clarifying lysates by centrifugation at 2,000 rpm for 1 min at 4 °C twice ...
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bioRxiv - Neuroscience 2022Quote: ... previously equilibrated in Wash buffer 1 (50 mM Tris-HCl pH 7.5, 150 nM NaCl, 1% NP-40, 1x protease inhibitors (Roche)) for 3 times ...
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bioRxiv - Cancer Biology 2022Quote: ... whole-cell extracts were prepared by lysing cells in buffer X (50 mM Tris pH 8.5, 250 mM NaCl, 1 mM EDTA, 1% NP-40, protease inhibitor minitablet (Roche)) and quantified using Bradford assay (Bio-Rad) ...
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bioRxiv - Genomics 2022Quote: ... before being re-pelleted by centrifuging for 3 minutes at 4°C at 500g and resuspended in 180µl TMS buffer (10mM Tris-HCl pH 8, 1mM MgCl2, 1% SDS, 1× Complete Protease Inhibitor Cocktail (PIC, Roche)) with 0.4µl benzonase (E1014 ...
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bioRxiv - Immunology 2022Quote: ... the cells were lysed with ChIP-Lysis buffer (10 mM EDTA, 1% [w/v] SDS, 50mM Tris-HCl, pH 7.5, 1 tablet Protease Inhibitor Cocktail [#11836170001; Roche, Mannheim ...
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bioRxiv - Plant Biology 2022Quote: ... 150mM NaCl, 1mM EGTA, 50mM HEPES, pH 7.5, 1 mM PMSF and 1× cOmplete protease inhibitor cocktail from Roche). 30 μl GST beads (Glutathione Sepharose 4 Fast Flow ...
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bioRxiv - Plant Biology 2022Quote: The samples were ground in liquid nitrogen and homogenized in the extraction buffer (100 mM Tris-HCl pH 8.8, 150 mM NaCl, 1 mM EDTA, 20% glycerol, 1 mM PMSF, 1x cOmplete protease inhibitor cocktail from Roche, 1x phosphatase inhibitor cocktail 2 and 3 from Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell pellets were lysed in lysis buffer (150 mM NaCl, 50 mM HEPES pH 7.6, 1 mM EDTA, 2 mM MgCl2, 1% Triton X, 10% Glycerol, 1X Roche cOmplete EDTA-free Protease Inhibitor ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was transferred to a blocking buffer (1 M maleic acid solution pH7.4, 1% nucleotide blocking reagent (Roche)) for 30 minutes and then incubated in antibody-solution (anti-dioxigenin-AP fragments in blocking buffer) ...
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bioRxiv - Molecular Biology 2022Quote: ... was added to the indicated samples in Pull-down Buffer 1 containing 1% (w:v) BSA supplemented with cOmplete EDTA-free protease inhibitor cocktail (Roche) (final volume ...
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bioRxiv - Molecular Biology 2022Quote: ... then resuspended in cytoplasmic lysis buffer (25 mM Tris–HCl pH 7.5, 10 mM NaCl, 1.5 mM MgCl2, 1% IGEPAL CA-630, and 1× protease inhibitor cocktail [“PI”, Roche 11836170001]) at 4°C and incubated on ice for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... the cell pellet was resuspended in 10 mL of column buffer (20 mM Tris-HCl pH 7.5, 200 mM NaCl, 1 mM EDTA, protease inhibitors [1 Roche Mini EDTA-free tablet] ...
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bioRxiv - Plant Biology 2024Quote: ... were ground in liquid N2 and 100 µL extraction buffer added (125 mM Tris-Cl pH 8.8, 1% SDS, 10% glycerol, 1 mM PMSF and Complete Protease Inhibitor [Roche]). Samples were centrifuged at 7000 g for 10 min and supernatant collected ...
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bioRxiv - Plant Biology 2024Quote: ... Root tissues were ground with liquid nitrogen in a mortar before homogenization with the extraction solution (20 mM Tris-HCl, pH 7.0, 1 mM EDTA, 1 mM DTT and a cocktail of protease inhibitors, Roche). The suspension was ultracentrifuged at 105,000 g for 20 min ...
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bioRxiv - Neuroscience 2024Quote: ... they were incubated for 1 h at room temperature (20– 25 °C) in 0.1 M PBS with 1% blocking reagent (Roche) and 0.3% Tween 20 (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2024Quote: ... The cells were harvested and washed thrice with FACS washing buffer (1X PBS + 1 mM EDTA, pH 7.2 + 1 Complete Inhibitor EDTA-free tablet (Roche) per 50 mL buffer) ...
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bioRxiv - Microbiology 2024Quote: ... resuspended in 1 ml cytoplasmic lysis buffer (25 mM Tris–HCl pH 7.5, 10 mM NaCl, 1.5 mM MgCl2, 1% IGEPAL CA-630, and 1× protease inhibitor cocktail [“PI”, Roche 11836170001]) at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were resuspended in a 1:1 ratio (w/v) in PB supplemented with Complete Protease Inhibitor Cocktail (Roche) and afterward dropwise frozen in liquid nitrogen before lysed in 6875D LARGE FREEZER/MILL (SPEX SamplePrep LLC) ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were prepared by resuspending cell pellets in 2 mL NP-40 buffer (50 mM Tris-HCl pH 7.8, 150 mM NaCl, 1% NP-40, 1 mM PMSF, 1x PhosSTOP [Roche]), sonication on ice (5s on/10s off for 2 mins at 40% amplitude) ...
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bioRxiv - Neuroscience 2023Quote: ... cells were incubated in staining solution (1 M Tris-HCl pH 9.5, 5 M NaCl, 1 M MgCl2, NBT-BCIP, H2O) (Roche) in the dark for 10 min at room temperature ...
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bioRxiv - Genomics 2022Quote: ... and incubated overnight with primary antibody in 3% BSA/TBS-T (monoclonal mouse anti-FLAG, 1:1,000; Sigma-Aldrich, #F1804-1MG, RRID:AB_262044 or monoclonal mouse anti-GFP, 1:1,000, Roche #11814460001, RRID:AB_390913) at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Mitochondrial pellets were solubilized in lysis buffer with 1 % digitonin (25 mM HEPES pH 7.5, 150 mM NaCl, 5 % glycerol, 1 mM TCEP, supplemented with PhosStop (Roche), cOmplete protease inhibitor cocktail EDTA-free (Roche) ...
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bioRxiv - Cell Biology 2023Quote: 12-16 hours old embryos were collected and lysed in homogenization buffer (25 mM Hepes pH 7.4; 150mM NaCl; 0.5mM EDTA pH 8.0; 1 mM DTT and 1 tablet of proteinase inhibitor cocktail; Roche 11836170001). The aqueous phase of the lysate was collected after 1 hour of centrifugation at 4°C and centrifuged again for 25 minutes ...
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bioRxiv - Microbiology 2023Quote: ... The pellet was washed twice with 300 μl sodium phosphate buffer (20 mM Na-phosphate pH 6.8, 1 mM PMSF, 1× Mini Protease Inhibitor Cocktail, Roche) with centrifugation at 16,000g for 20 min between washes ...
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bioRxiv - Microbiology 2023Quote: ... and resuspended in lysis buffer (50 mM PBS pH 7.4, 1 mM EDTA, 5% glycerol, 1 mM PMSF and protease inhibitors from Roche). Cell lysis and protein extraction was achieved through mechanical lysis using glass beads and Precellys®24 Homogenizer (3 cycles of 30 seconds at 5500 rpm ...
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bioRxiv - Molecular Biology 2023Quote: ... 140 mM NaC1, 1 mM EDTA, 10% glycerol, 0.1% NP-40, 1 mM PMSF, and 1x protease inhibitor cocktail [Complete; Roche]). The cell pellet was flash frozen in liquid nitrogen with 1.5 mL of lysis buffer and grounded in a Spex Freezer Mill 6775 to a fine powder ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then blocked for 1 h and incubated with an anti-DIG antibody (Roche 11093274910, RRID:AB_514497, 1:2000) diluted in MABT buffer (0.1 M maleic acid pH 7.5 ...
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bioRxiv - Immunology 2024Quote: ... Cells were lysed in RIPA buffer (50 mM Tris HCL, 150 mM NaCl, 1% SDS, 0.5% Na-deoxycholate, 1× Complete protease inhibitor (Roche)) for 30 min on ice ...
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bioRxiv - Cell Biology 2024Quote: ... Coverslips were incubated with CENP-B box probe-Cy3 (PNAbio) 1:1 in hybridization buffer (20mM Tris, pH 7.4, 60% formamide, 0.5% of blocking reagent (Roche 11096176001)) ...
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bioRxiv - Neuroscience 2021Quote: ... Nematodes were resuspended in 8 M urea in 100 mM TRIS-HCl buffer (pH 8) containing cOmplete™ protease inhibitor cocktails (Roche Diagnostic Canada, Laval, QC, Canada) and aliquoted into reinforced 1.5 mL homogenizer tubes containing 500 μm glass bead homogenizer tubes with 25 mg of glass beads ...
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bioRxiv - Developmental Biology 2019Quote: ... 100 µl 10% SDS, 500 µl 20% N-Lavroyl sarsosine sodium, 2 tablets of cOmplete ULTRA Protease Inhibitor Cocktail [Roche Cat.# 05892970001] in 10ml FA buffer). The sample was then sonicated using a Covaris S220 at the following settings ...
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bioRxiv - Developmental Biology 2022Quote: ... 100 µl 10% SDS, 500 µl 20% N-Lauroyl sarsosine sodium, 2 tablets of cOmplete ULTRA Protease Inhibitor Cocktail [Roche Cat.# 05892970001] in 10ml FA buffer). For each IP experiment ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then lysed using 0.4 mL lysis buffer containing 2x protease inhibitors (Complete, Mini, EDTA-free; Roche), 1x phosphatase inhibitors (PhosStop ...
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bioRxiv - Molecular Biology 2021Quote: ... Final DNA concentration of 1μM and increasing concentrations of Stl (0.5-8 μM) were mixed with 1μg/ml poly(d[I-C]) (Roche) in EMSA buffer (50 mM Tris-HCl pH 8 ...
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bioRxiv - Molecular Biology 2022Quote: ... The nuclei were resuspended at 1 million nuclei per mL in wash buffer (20mM HEPES pH 7.9, 150mM NaCl, 0.5mM Spermidine supplemented with 1x Roche cOmplete mini ...
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bioRxiv - Physiology 2022Quote: ... Digestion was continued by adding 200 μL of LiberaseTM TL Research Grade (0.25 mg/mL; Hoffmann-La Roche) per 2 mL of cell isolation solution ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were transferred to PVDF membranes and labelled overnight with anti-HA (0.01 μg/mL, Roche clone 3F10), anti-VE-Cadherin (0.5 μg.ml−1 ...
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bioRxiv - Microbiology 2019Quote: ... Cells were lysed with lysis buffer (final concentration: 50 µg/ml lysozyme, 0.8% NP40, 1X protease inhibitor (Roche), 250 U/ml benzonase and 1 mM MgCl2 in PBS ...
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bioRxiv - Genomics 2019Quote: ... Cell nuclei pellet was re-suspended in 2 mL FACS buffer (0.15 mM spermine, 0.5 mM spermidine, Complete protease inhibitor (Roche) and 20mM Na-butyrate (Sigma ...
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bioRxiv - Cancer Biology 2019Quote: ... Purified carcinoma organoids were dissociated into single cell suspension in 0.05% trypsin with 0.1% EDTA supplemented with 2 U/ml of DNase I (Roche) for 2-3 min ...
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bioRxiv - Immunology 2021Quote: ... Individual intact lung lobes were cut away and additionally kept in a dispase solution (50 U/ml, Roche) for 45 min at room temperature on a rocker at 150 rpm ...
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bioRxiv - Neuroscience 2021Quote: ... Trypsin was aspirated and the digestion was stopped by adding 0.5 mg/mL DNase I (Roche, Basel, Switzerland) in microglia medium [Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Systems Biology 2021Quote: ... the animals were perfused (1.5 minutes, 4.5 ml/min) with ice-cold isotonic saline containing protease inhibitors (Roche cOmplete™ Mini ...
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bioRxiv - Plant Biology 2021Quote: ... and 0.33 M D-sorbitol supplemented with one tablet (per 50 mL) of cOmplete protease inhibitor cocktail (Roche)] using a Waring blender ...