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Citations for Roche :
5801 - 5850 of 8835 citations for FH1 FH2 domain containing protein 1 FHOD1 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The slices were lysed in lysis buffer (50 mM Tris/HCl, 1 mM EDTA, 1% SDS, phosphatase inhibitor cocktail (Roche, Indianapolis, IN, USA), and protease inhibitor cocktail (Roche) ...
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bioRxiv - Microbiology 2023Quote: ... and resuspended in 1 mL of buffer 1 (20 mM K-HEPES pH 7.9, 50 mM KCl, 10% glycerol, and Roche EDTA-free protease inhibitors). Subsequently ...
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bioRxiv - Biophysics 2023Quote: ... 1% Triton X-100, 10% glycerol, 10 mM NaH2PO4, 10 mM NaP2O7, 100 μM ZnCl2, 10 mM NaF, 1 Roche protease inhibitor tablet), lysed by sonication and the supernatant isolated by centrifugation ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 M NaCl, 30 mM imidazole, 1 mM DTT, 1 mM benzamidine, and one EDTA-free complete protease inhibitor cocktail tablet [Roche] per 50 ml). The lysate was clarified by centrifugation at 38,000 g in a fixed-angle rotor ...
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bioRxiv - Cell Biology 2024Quote: ... pH 8, 150 mm NaCl, 1 mm EDTA, 1% Triton X-100, and cOmplete™ EDTA-free Protease Inhibitor Mixture, Roche Applied Science) before clarification by centrifugation and enrichment for FLAG-tagged proteins using anti-FLAG® M2 Affinity Gel (A2220 ...
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bioRxiv - Plant Biology 2024Quote: ... The immunoprecipitated proteins were then separated via 13.5% SDS-PAGE, blotted, and analyzed by immunoblotting using anti-GFP (TransGen, HT801, 1:1,000 dilution) and Anti-HA-Peroxidase (Roche, 3F10, 1:500 dilution) antibodies.
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bioRxiv - Cell Biology 2024Quote: ... 1 x 108 HeLa cells expressing ABHD11-Flag were lysed in 1ml lysis buffer (50mM Tris-HCl pH7.4, 150mM NaCl, 1% Triton-×100, 1× Roche cOmplete protease inhibitor cocktail) and rotated at 4 °C for 1 hr ...
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bioRxiv - Developmental Biology 2021Quote: ... FISH signal was developed with tyramide reaction following O/N incubation of embryos in sheep anti-DIG antibody (Roche; Cat#11222089001) (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... Thee injections of either an anti-GluA2 antibody (clone 15F1, gift from E. Gouaux) or a monoclonal anti-GFP IgG1-K (Roche, 11814460001) were targeted to the L2/3 of S1 (−0.1 to 0.3 mm dorsoventral) ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then cultured with 2 μg/ml of plate-bound anti-CD3 and anti-CD28 monoclonal antibodies (αCD3αCD28 stimulation) (mAbs) (eBioscience) and 25 U/ml of recombinant human interleukin-2 (IL-2; Roche Applied Science) at a concentration of 1.5-2 × 106 cells/ml in RPMI supplemented with 10% heat-inactivated Human Serum (HS ...
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bioRxiv - Microbiology 2022Quote: ... 50 µl of the post-nuclear supernatant was saved as the input lysate and 450 µl was incubated with 5 µg of anti-GFP antibody (Roche 11814460001) for 1 hour at 4°C ...
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bioRxiv - Genetics 2022Quote: ... 10% of cleared lysate was set aside to serve as input samples and the remainder was incubated at 4°C with anti-GFP antibodies (Roche #11814460001) for 3 h under gentle rotation ...
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bioRxiv - Microbiology 2022Quote: ... Immunoreactivity in the lung tissues was assessed using a rabbit anti-SARS-CoV-2 Nucleocapsid pAb as the primary antibody (Sinobiological, China) and further processed using a Ventana Discovery Ultra (Roche, USA) system.
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bioRxiv - Plant Biology 2020Quote: ... by standard SDS-PAGE and detection by chemiluminescence with a monoclonal Anti-GFP antibody (Mouse IgG1K, clones 7.1 and 13.1, Roche Cat. No. 11814460001) and Anti-Mouse secondary antibody HRP-conjugated (Amersham ECL GE Cat ...
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bioRxiv - Physiology 2020Quote: ... The incorporated digoxigenin-labelled nucleotides were detected by anti-digoxigenin-POD conjugated antibodies (Dilution 1.5 IU/ml; Roche, Cat no. 11633716001). Fluorescent signals were obtained by peroxidase-mediated Alexa Fluor 488 tyramide labelling (Alexa fluor 488 Tyramide SuperBoost kit ...
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bioRxiv - Immunology 2020Quote: ... Roche Elecsys® Anti-SARS-CoV-2 Immunoassay for the qualitative detection of antibodies (including IgG) against SARS-CoV-2 was used with a COBAS e411 analyzer (disk system; Roche, Switzerland) according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2022Quote: ... Total NUC/GEN polypeptide abundance was detected by probing duplicate blots with a mouse anti-GFP antibody (#11814460001; Sigma Aldrich (Roche), Darmstadt ...
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bioRxiv - Cancer Biology 2023Quote: ... unconcentrated cell culture supernatants were incubated with a rabbit monoclonal anti-glycodelin antibody (clone 12C9, kindly provided by ROCHE, Penzberg, Germany) for 1 h at room temperature prior to incubation with immune cells ...
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bioRxiv - Cancer Biology 2024Quote: ... The samples were developed by using either secondary antibodies linked to horseradish peroxidase with the UltraView™ Universal DAB Detection Kit (Ventana Medical System, Roche) or secondary antibodies linked to fluorophores ...
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bioRxiv - Cell Biology 2022Quote: ... 25 µL of these samples were resolved by SDS-PAGE and analyzed by western blotting with α-HA3 tag antibody (#3F10, Roche) for western blotting ...
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bioRxiv - Neuroscience 2023Quote: ... we incubated slices with the secondary antibody (Alexa 488 donkey-anti-chicken, Immuno Jackson, 703-545-155) and DAPI (Roche, 10236276001) for 2h at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... 50 µL of the post-nuclear supernatants was saved as the input lysate and 450 µL were incubated with 5 µg of anti-GFP antibody (Roche 11814460001) for 1 hour at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... and a Cy3 filter by detecting fluorescence emission using ImageQuant 800 Fluor CCD imager (Cytiva) followed by western blotting using a α-GFP antibody (Roche).
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bioRxiv - Genetics 2021Quote: ... with 3x protease inhibitors (cOmplete Protease Inhibitor Cocktail EDTA-free - 1 mM phenylmethylsulfonyl fluoride, 4 mM benzamidine, 2 μg/ml leupeptin, and 1 μg/ml pepstatin, Roche Diagnostics, cat. number 1187358001) and 3x phosphatase inhibitors (Millipore Inhibitor Cocktail Set ...
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bioRxiv - Molecular Biology 2021Quote: ... 150 mM NaCl, 1 mM EDTA, 1% v/v Triton X-100, 10% v/v glycerol, 1x cOmplete protease inhibitor cocktail [Roche], and 1x PhosSTOP [Roche]). Cells were scraped off ...
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bioRxiv - Developmental Biology 2020Quote: ... expressing HA-tagged TWIST1 interaction candidates were thawed in 300 μL hypotonic lysis buffer (HEPES 20 mM, MgCl2 1 mM, Glycerol 10%, Triton 0.5 %. DTT 1 mM, 1x Complete protease inhibitor [Roche], Benzo nuclease 0.5 μl/ ml) and incubated at room temperature for 15 mins (for nuclease activity) ...
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bioRxiv - Genetics 2020Quote: ... 0.1 M ammonium bicarbonate, pH 8, 150 mM NaCl, 1 Roche mini protease inhibitor tablet without EDTA/10 ml, ½ Roche phosSTOP tablet/10 ml) using 14 × 1.5 min bursts on a BioSpec mini bead-beater at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Pellets were thawed in one pellet volume of RP buffer (50 mM Tris pH 8.0, 300 mM NaCl, 1 mM MgCl2, 1 mM PMSF, Roche Complete protease inhibitor tablet 1x). Cells were opened using a French press (Constant Systems LTD ...
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bioRxiv - Neuroscience 2020Quote: ... for 30 min at 4°C on a rotator and S1 (1 ml for cerebellum and 1 ml for cerebrum) was incubated with 5 μg of anti-HA mAb (Roche, clone 12CA5, RRID: AB_514505) at 4°C for 60 min on a rotator ...
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bioRxiv - Genomics 2020Quote: ... Pellets were resuspended in 10 mL of L2 buffer (200mM NaCl 1mM EDTA pH 8 0.5mM EGTA 10mM Tris, pH 8, dH2O, 1 protease inhibitor tablet (Roche Complete cat #1 697 498) per 50ml buffer ...
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bioRxiv - Cell Biology 2020Quote: ... The buffers used in each step were supplemented with 1% (v/v) phosphatase inhibitor cocktail-2 and PhosSTOP (Roche: 1 tablet per 10 ml). Imaging of fixed samples was carried out on a Leica TCS SP8 MP microscope using oil immersion objective (HP CL APO CS2 63x/1.40 Oil) ...
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bioRxiv - Biochemistry 2024Quote: ... 50 mM NaCl, 0.1 mM EDTA, 10% [v/v] glycerol, 1% [w/v] digitonin, 2 mM PMSF, 1 x Roche EDTA free protease inhibitor cocktail). After removing the debris by a clarifying spin (12,000 x g ...
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bioRxiv - Plant Biology 2020Quote: ... 1 mM phenylmethylsulfonyl fluoride (PMSF) and protease inhibitor cocktail (Roche)) ...
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bioRxiv - Neuroscience 2021Quote: ... cell nuclei were counterstained with DAPI (1:2000, Roche, 10236276001) and sections embedded in fluorescent mounting medium (Dako ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 mM PMSF and protease inhibitor cocktail (Cat.11873580001, Roche). After pre-cleaned ...
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bioRxiv - Developmental Biology 2021Quote: ... Nuclei were stained using DAPI at 1 μg/mL (Roche) for 3 min.
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bioRxiv - Cell Biology 2020Quote: ... 1 tablet of complete EDTA-free protease cocktail inhibitor (Roche)) ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM β-mercaptoethanol) supplemented with Complete Protease Inhibitor (Roche). After homogenization ...
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bioRxiv - Cell Biology 2020Quote: ... 1% Nonidet P-40] supplemented with protease inhibitor mixture (Roche) for 20 min on ice ...
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bioRxiv - Developmental Biology 2021Quote: ... 1% Nonidet P-40) supplemented with protease inhibitors cocktail (Roche) and 1mM PMSF ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 Complete Mini EDTA-free Protease Inhibitor Cocktail tablet (Roche)) ...
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bioRxiv - Developmental Biology 2020Quote: ... samples were incubated with sheep-anti-DIG (1/1000, Roche) followed by biotinylated donkey-anti-sheep (1/500 ...
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bioRxiv - Developmental Biology 2020Quote: ... and 1 tablet/50 ml protease inhibitor cocktail tablet (Roche). After centrifugation at 13,000 g for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1 x EDTA-free Complete Protease Inhibitor Cocktail (Roche). After brief sonication ...
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bioRxiv - Microbiology 2022Quote: ... an extra 1 µl of 10 mM dideoxynucleoside triphosphate (Roche) was added to replace 1 µl of nuclease-free water ...
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bioRxiv - Neuroscience 2021Quote: ... 1:500 anti-fluorescein-POD (Roche (11 426 346 910)) 1/2000 ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 x Complete ULTRA EDTA-free protease inhibitor mix (Roche), 1 x HALT Phosphatase inhibitor mix (Thermo ...
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bioRxiv - Molecular Biology 2021Quote: ... 1% NP-40 supplemented with COMPLETE protease inhibitor cocktail (Roche) and Benzonase®Nuclease (Sigma ...
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bioRxiv - Plant Biology 2020Quote: ... 1 mM DTT) supplemented with Complete Protease Inhibitor Cocktail (Roche). Cells were disrupted by sonication ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by a 1 hr treatment with DNAse I (Roche) prior to purification of the newly synthesize RNA on Quick Spin Columns (Roche ...