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Citations for Roche :
5751 - 5800 of 6945 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade D UG28R since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: 7-day-old Q28 and Q69 seedlings were lysed in lysis buffer (1% Triton X-100, 50 mM Tris–HCl pH 8.0) supplemented with protease inhibitor cocktail (Roche) and 25 mM N-ethylmaleimide ...
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bioRxiv - Molecular Biology 2022Quote: ... 60 mM KCl, 15 mM NaCl, 1 mM CaCl2, 0.9% Triton X-100, and 13 protease inhibitor cocktail [Roche]). After homogenization ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 mM EDTA, 0.5% NP-40, 1 mM DTT, 1X protease inhibitor [EDTA-free complete Protease Inhibitor Cocktail, Roche, 11873580001] and 1:100 phosphatase inhibitor [Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... cell pellets were lysed in whole-cell extraction buffer (150 mM NaCl, 50 mM Tris pH 7.5, 1% NP-40) supplemented with proteinase and phosphatase inhibitors (Roche). The lysates were cleared by centrifugation at 14,000 rpm for 30 min at 4 °C ...
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bioRxiv - Plant Biology 2022Quote: ... ground in a fine powder with liquid nitrogen and resuspended with 1 ml of Guanidine hydrochloride extraction buffer (6M GuaHCl, HEPES 1 M, EDTA: 5 Mm, adjusted to pH 7.5) supplemented with protease inhibitor cocktail (Roche). Homogenates were filtered through a 100 µm cotton mesh ...
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bioRxiv - Molecular Biology 2023Quote: ... membranes were incubated overnight at 4°C with monoclonal mouse anti-GFP antibody (Roche; Basel, CH; dilution 1:500) or monoclonal rat anti-HA antibody (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... Dissected cortexes were collected in 24 well-plate and cut into small pieces to be digested in 1.5 ml of digestion buffer containing 1 mg/ml of collagenase/dispase cocktail (Roche) and 0.5 mg/ml of DNase I ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were transfected according to the manufacturer’s protocol at a µL lipofectamine: µg plasmid ratio of 3:1 (X-tremeGENE 9, Roche). After 48 hours ...
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bioRxiv - Plant Biology 2023Quote: ... in 100μl GTEN-buffer (10% Glycerol, 25mM TRIS pH 8, 0.5mM EDTA, 150 mM NaCl,1 x protease inhibitors (Roche), 5mM DTT ...
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bioRxiv - Plant Biology 2023Quote: ... A GTEN-based buffer (10% Glycerol, 25mM TRIS pH 8, 0.5mM EDTA, 150 mM NaCl, 1 x protease inhibitors (Roche), 5mM DTT ...
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bioRxiv - Microbiology 2023Quote: ... Cells were resuspended in 1000 µL cold cytoplasmic lysis buffer (10 mM HEPES pH 7.9, 10 mM KCl, 2 mM Mg acetate, 3 mM CaCl2, 340 mM sucrose, 1 mM DTT, Roche protease inhibitor cOmplete tablets EDTA to 1× ...
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bioRxiv - Microbiology 2023Quote: ... cells were resuspended in 1000 µL cold sucrose buffer containing (10 mM HEPES pH 7.9, 10 mM KCl, 2 mM Mg acetate, 3 mM CaCl2, 340 mM sucrose, 1 mM DTT, Roche protease inhibitor cOmplete tablets EDTA to 1× ...
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bioRxiv - Microbiology 2023Quote: ... Supernatants were collected as the cytoplasmic fraction and pellets were resuspended in 200 µL cold nuclear lysis buffer (20 mM HEPES pH 7.9, 100 mM NaCl, 1 mM DTT, Roche protease inhibitor cOmplete tablets EDTA free ...
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bioRxiv - Plant Biology 2022Quote: ... and vortexed vigorously in extraction buffer 1 (50 mM Tris–HCl pH 7.5, 150 mM NaCl, Complete EDTA-free Protease Inhibitor cocktail (Roche), PhosSTOP phosphatase inhibitor cocktail (Roche) ...
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bioRxiv - Immunology 2023Quote: ... the hydrogels were carefully collected using cut 1000-µL pipette tips and incubated 15 minutes at 37°C in a 0.5 mg.mL-1 Collagenase (Roche, 11088866001) solution in DMEM/F12 ...
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bioRxiv - Neuroscience 2022Quote: ... Biotinylated cells were scraped into lysis buffer (10 mM Tris pH 7.6, 1% Triton X-100, 150 mM NaCl, protease inhibitor (complete, without EDTA (Roche), ½ tablet per 20 mL) ...
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bioRxiv - Neuroscience 2022Quote: ... mice brain/cells were lysed in lysis buffer (20 mM Tris pH=7.5, 150 mM NaCl, 1% Triton X-100) containing protease inhibitor cocktail (Roche). Lysate was sonicated and incubated at 4 °C for 30 min while rotating ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclei were resuspended in nuclei lysis buffer (5mM Tris-HCl, pH 8.0, 10mM EDTA, 1% SDS in 1X PBS, 1X Roche Complete protease inhibitors ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... 0.5% Triton X-100) supplemented with fresh protease and phosphatase inhibitors (1 mM PMSF, 0.1 mM Na3VO4 and 1x PIC - Roche). Cell lysates were passed through a 1 ml syringe for mechanical disruption and centrifuged for 10 min at 13.000 rpm ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... 0.5% Triton X-100) supplemented with fresh protease and phosphatase inhibitors (1 mM PMSF, 0.1 mM Na3VO4 and 1x PIC - Roche), followed by three washes with kinase buffer (25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Plant Biology 2022Quote: ... 150 mM NaCl, 1 mM EDTA, 20% glycerol, 0.2% Triton X-100, and 1x cOmplete protease inhibitor cocktail from Roche, 1x phosphatase inhibitor cocktail 2 and 3 from Sigma ...
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bioRxiv - Physiology 2022Quote: ... and resuspended in 600 μL of cold nuclear lysis buffer (10 mM Tris pH 8, 1 mM EDTA, 0.5 mM EGTA, 0.3% SDS, 1X cOmplete™ Protease Inhibitor Cocktail, Roche) for 30 min on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... Triton X-100 in PBS for 20 min and followed by 1 hr DNAse (1,000 U/ml; #04536282001, Roche) treatment at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... cells were lysed in 8 M urea buffer (8 M urea, 1 M Tris pH 7.4, 5 M NaCl) containing protease and phosphatase inhibitors (Roche) followed by sonication at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Pelleted cells were resuspended in Lysis buffer (50mM Tris pH 7.4, 150mM NaCl, 5mM MgCl2 1% Triton X-100, protease inhibitors (Roche Complete Inhibitor cocktail ...
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bioRxiv - Molecular Biology 2024Quote: ... and resuspended in 300 µL cold nuclear lysis buffer (1% SDS, 10 mM EDTA, 50 mM tris-HCl pH8.0, 1x protease/phosphatase inhibitor, Roche) and lysed (10 min on ice) ...
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bioRxiv - Molecular Biology 2024Quote: ... and resuspended in 50 µL cold nuclear lysis buffer (1% SDS, 10 mM EDTA, 50 mM tris-HCl pH8.0, 100U Ribolock inhibitor, Thermo, 1x protease/phosphatase inhibitor, Roche) and lysed (10 min ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed twice with DPBS, resuspended in lysis buffer (1% NP40 in PBS, pH 7.4 with protease inhibitor cocktail (Roche), and 2.5% v/v RNase inhibitor) ...
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bioRxiv - Biochemistry 2024Quote: Homogenized lungs were 10-fold diluted starting 1:10 in infection media (DMEM containing 0.1% BSA (fraction V; Roche), 10 mM HEPES (Corning) ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.1 % SDS, 150 mM NaCl, 10 mM EDTA, 20 mM Tris-HCl pH7.5 and complete protease inhibitor mix, Roche) was used to prepare protein lysates ...
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bioRxiv - Biochemistry 2023Quote: ... after removal of the medium cells were lysed in 1 mL of lysis buffer (50 mM Hepes [pH 8.0], 150 mM NaCl, 1 mM DTT, 5 mM MgCl2, 5 % Glycerol, supplemented with 0.55 % Nonidet P40 substitute [Roche] and cOmpleteTM EDTA-free Protease Inhibitor Cocktail [Roche]) ...
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bioRxiv - Neuroscience 2024Quote: ... and blocked for 1 hour in blocking buffer consisting of 20% sheep serum and 2% blocking reagent (11096176001, Roche) in MABT ...
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bioRxiv - Biochemistry 2023Quote: ... the membrane was incubated with 1 nM DIG (Digoxigenin)-labeled Telomere probes in Hybridization solution (DIG Easy Hyb, Roche) at 42°C ...
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bioRxiv - Biochemistry 2024Quote: ... pooled and resuspended in ice-cold lysis buffer (150 mM NaCl, 50 mM Tris pH 8.0, 1 × cOmplete EDTA-free protease inhibitor (Roche), 1 mg/mL DNase and lysozyme ...
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bioRxiv - Biochemistry 2024Quote: ... Frozen cell pellets were lysed in immunoprecipitation lysis buffer (50 mM Tris [pH = 7.5], 150 mM NaCl, 1% IGEPAL, 10% Glycerol) with cOmplete Protease Inhibitor Cocktail (Roche) and PhosSTOP Phosphatase Inhibitor Cocktail (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... cells were detached using cold PBS and lysed in 350 µl lysis buffer (50 mM Tris pH 8.0, 150 mM NaCl, 1% Triton X-100) containing protease inhibitor (Roche) for at least 20 min on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in 1x PLB (10x PLB: 1 M KCl, 50 mM MgCl2, 100 mM HEPES-NaOH pH 7.5, 5% NP-40, Roche protease and phosphatase inhibitors (1 tab each per 10 mL) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in 1x PLB (10x PLB: 1 M KCl, 50 mM MgCl2, 100 mM HEPES-NaOH pH 7.5, 5% NP-40, Roche protease and phosphatase inhibitors (1 tab each per 10 mL) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in 1x PLB (10x PLB: 1 M KCl, 50 mM MgCl2, 100 mM HEPES-NaOH pH 7.5, 5% NP-40, Roche protease and phosphatase inhibitors (1 tab each per 10 mL) ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were washed twice in ice-cold PBS and resuspended in 600 uL NLB (1xPBS, 300 mM NaCl, 1% TritonX-100, 0.1% Tween-20) freshly supplemented with 1x Complete protease inhibitors (Roche), and 1x PhosSTOP (4906845001 ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK cells were lysed in 1xPBS pH 7.4 containing Protease Inhibitor Cocktail tablet (1 tablet/10 mL) (Roche Diagnostics) and separated in 4–16% gradient Native pre-cast gel (SERVA Electrophoresis GmbH) ...
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bioRxiv - Developmental Biology 2024Quote: ... 5 ng amplified DNA and 1 μM forward and reverse primers and run on a LightCycler 480 machine (Roche) using the following protocol ...
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bioRxiv - Neuroscience 2023Quote: ... The heads were crushed on ice using a pestle in 0.5% NP-40 lysis buffer (50 mM Tris, 150 mM NaCl, 10% glycerol, 1 mM EDTA, pH 7.5) supplemented with protease inhibitors (Roche). The resulting lysates were clarified by centrifugation and the supernatants were collected ...
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bioRxiv - Molecular Biology 2024Quote: ZNF274 expression was induced with 1µg/ml doxycycline and verified via western blot with an anti-HA antibody (1:1000, ref.12013819001, Roche) and anti-beta actin antibody (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... then transferred for 48 hrs in a solution of mouse anti-synaptophysin (1:20, Roche Applied Science, Indianapolis, IN). Sections were then washed in PBS-t 3x10 min ...
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bioRxiv - Molecular Biology 2024Quote: Cells were harvested and protein was extracted using lysis buffer (20mM HEPES pH7.4, 2mM MgCl2, 40mM NaCl and 1% Triton-X) containing complete mini EDTA protease inhibitor (Roche) at 1 in 50 concentration ...
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bioRxiv - Molecular Biology 2024Quote: ... The crude nuclear pellet was resuspended in nuclear lysis buffer (50 mM Tris-HCl, pH 8.0, 10 mM EDTA, 1% SDS, and Complete protease inhibitor; Roche) and sonicated with a Branson sonifier (VWR ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cell viability was determined by using the WST-1 assay according to the manufacturer’s instructions (Roche Diagnostics, Mannheim, Germany). After removing the cell supernatants for cytotoxicity measurements ...
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bioRxiv - Genetics 2024Quote: Tissues were homogenized in 500uL RIPA buffer (10% sucrose, 1% SDS, 5mM HEPES pH 7.9, 10mM sodium butyrate in MilliQ water supplemented by protease inhibitor [Roche 04693124001] ...
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bioRxiv - Developmental Biology 2024Quote: ... ovaries were crosslinked in 250 µL YSS buffer (50 mM pH 8.0 Tris HCl, 50 mM NaCl, 0.1% NP40, 1 U/mL of RNAsin (Roche, 3335402001), protease inhibitor (Thermo Scientific ...