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Citations for Roche :
5701 - 5750 of 6383 citations for PD 1 Mouse HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: ... The final pellet was resuspended in 750 uL nuclear lysis buffer (10 mM EDTA, 1% SDS, 50 mM Tris-HCl at pH 8, 1x cOmplete protease inhibitor cocktails (Roche)) ...
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bioRxiv - Developmental Biology 2024Quote: ... blocked in MAB-Buffer2 (20% sheep serum, MAB-Buffer1) for 1h and incubated with anti-DIG-AP antibody (1:4000, Roche) in MAB-Buffer2 overnight at 4 °C.
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bioRxiv - Genomics 2023Quote: ... and collected in lysis buffer (1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS and protease inhibitor [#11873580001, Roche] in PBS). Chromatin was sonicated with a Covaris E220 sonicator (140PIP ...
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bioRxiv - Immunology 2024Quote: ... Lungs were resected and minced with scissors before incubation in a collagenase buffer containing 1 mg/mL Collagenase-A (Roche), 2000U DNaseI (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... and lysed with sonication in lysis buffer (50mM Hepes, 500mM NaCl, 1% Tween20, 5% Glycerol, half a tablet of cOmplete EDTA-free protease inhibitor cocktail [Roche]). The suspension was sonicated at 150J in intervals of 1.5secs for 20 sec total in ice ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were harvested after 48 hours and lysed in 50 mM Tris pH 7.5, 150 mM NaCl, 1% Triton X-100, protease inhibitor (cOmplete™, Mini, EDTA-free (Roche)) ...
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bioRxiv - Biochemistry 2024Quote: ... the proteins were transferred to a PVDF membrane and immunodetected with an anti-Myc-HRP antibody (Myc-HRP, 1:5000, Roche). The remaining 10% of the immunoprecipitated samples together with 50 μg of the input were subjected to the same procedure but probed with an anti-GFP antibody (JL-8 ...
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bioRxiv - Bioengineering 2024Quote: cDNA from cells was diluted 1:10 and qPCR was performed in triplicate using LightCycler 480 SYBR Green I Mastermix (Roche). Samples containing primers specific for lmna ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were disrupted in 1% NP-40 lysis buffer (140 mM NaCl, 10 mM Tris-HCl pH 8, 1% NP-40) supplemented with proteinase inhibitors (Roche). Proteins were separated by electrophoresis (SDS-PAGE) ...
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bioRxiv - Cancer Biology 2024Quote: ... Membrane lysis was performed in 5Lml LB1 (50LmM HEPES pH 7.5, 140LmM NaCl, 1LmM EDTA, 10% glycerol, 0.5% 1% IPEGAL-CA630, 0.25% Triton X-100, and Roche protease inhibitors 11836170001) for 10 min at 4 °C with rotation ...
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bioRxiv - Immunology 2023Quote: ... The cell pellets were treated with lysis buffer (50 µg/mL digitonin, 75 mM NaCl, 1 mM NaH2PO4, 8 mM Na2HPO4, 250 mM sucrose, and Roche cOmpleteTM protease inhibitor cocktail ...
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bioRxiv - Neuroscience 2024Quote: ... sections were blocked for 30 min and then incubated in an alkaline phosphatase conjugated anti-DIG antibody (1:600, Roche). Slides were then washed and developed overnight in BCIP/NBT chromogen (Perkin Elmer) ...
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bioRxiv - Neuroscience 2023Quote: ... then lysed on ice for 15 minutes in non-denaturing lysis buffer (150 mM KCl, 10 mM MgCl2, 5mM HEPES, 1% IGEPAL) supplemented with protease inhibitors (Roche Complete EDTA-free ...
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bioRxiv - Bioengineering 2024Quote: ... livers were perfused at low flow for about 10 min with digestion buffer (low-glucose DMEM supplemented with 1 mM Hepes) containing freshly added Liberase (32 μg/ml; Roche). Digestion was stopped using isolation buffer (low-glucose DMEM supplemented with 10% FBS) ...
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bioRxiv - Immunology 2023Quote: ... 1 mM EDTA) and then detected with 2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium (ABTS) substrate (Roche Diagnostics) diluted at 1:1000 in H2O2 ...
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bioRxiv - Immunology 2023Quote: ... 1 mM EDTA) and then detected with 2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium (ABTS) substrate (Roche Diagnostics) diluted at 1:1000 in H2O2 ...
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bioRxiv - Neuroscience 2023Quote: ... 2 mM MgCl2 and 1 mM DTT supplemented with DNase I (10 µg/ml) and complete protease inhibitor cocktail tablets (Roche). Complex formation was initiated by adding synthesized Stachel peptide (100 µM ...
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bioRxiv - Pathology 2023Quote: ... Spain) (1:10000) for 1.5h in darkness was followed by the addition of HRP substrate (11112422001, Roche Applied Science, Germany). Absorbance was measured using NanoQuant Infinite M200 Pro multi-plate reader (Tecan ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... in PBS for 30 min and incubated overnight at 4°C with 1:1,500 anti-HA high affinity (Roche, 3F10). After three PBS washes ...
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bioRxiv - Plant Biology 2023Quote: ... The qRT-PCR was set up as a 10μL reaction using SYBR® Green 1 480 Lightcycler® Master (Roche) in a 384-well plate ...
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bioRxiv - Evolutionary Biology 2023Quote: Antisense probes were synthesized using purified PCR products (1 μg per reaction) (supplementary table S16) and DIG RNA labeling Kit (T7) (Roche), following the manufacturer instructions ...
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bioRxiv - Cell Biology 2022Quote: HK cells were lysed using a lysis buffer (50mM Hepes, 130mM NaCl, 1mM DTT 1% NP-40) with complete protease inhibitor cocktail (Roche). Cell lysates were centrifuged at 13,000 rpm for 10 mins at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... the supernatant was centrifuged at 20000 g for 30 min at 4°C and the resulting pellet was solubilized with lysis buffer (20 mM Tris–HCl pH 8, 150 mM NaCl, 1% TritonX100, Complete EDTA-free Protease Inhibitor cocktail (Roche), PhosSTOP phosphatase inhibitor cocktail (Roche) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and then subjected to a transcardial perfusion with calcium-free Tyrode’s solution containing protein phosphatase-inhibitors (+PPIs) (1 tablet PhosSTOP per 10 ml) (Roche 04906845001) followed by Zamboni’s fixative containing 4% paraformaldehyde and 0.2% picric acid in 0.1 M phosphate buffer pH 7.4 +PPIs ...
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bioRxiv - Microbiology 2022Quote: Lungs were perfused with sterile PBS and digested at 37°C for 1 h with 625 μg/mL collagenase D (Roche) and 75 U/mL DNase I (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... cells were washed with ice-cold PBS and scraped into lysis buffer (1 mM EPPS, 8 M urea, protease (complete mini, EDTA-free) inhibitors (Roche) and PhosSTOP phosphatase inhibitor (Roche)) ...
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bioRxiv - Neuroscience 2023Quote: ... Five P2 mouse brains were homogenized in 5 mL of homogenization buffer (5 mM Tris-HCl, 0.32 M sucrose, 1 mM MgCl2) supplemented with EDTA-free protease inhibitors (cOmplete™, Roche), then centrifuged at 1,660 x g for 15 min at 4 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... blots were hybridized with 10 pmol/ml DIG-labeled (CAG)7 (5′-gcAgCagcAgca-3′) at 70°C for 4 h in hybridization buffer (5 × SSC, 1% block solution [Roche, Switzerland] ...
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bioRxiv - Cell Biology 2023Quote: Whole kidneys were homogenized and protein was extracted from 20 mg of tissue in 1 ml RIPA buffer supplemented with protease inhibitors (Roche) for 2 hrs ...
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bioRxiv - Cancer Biology 2022Quote: Frozen bacteria pellets were thawed on ice and loosened in 25 mL lysis buffer (20 mM Tris-HCl, 150 mM NaCl, 1% Triton X100, 10 mM DTT, 1X Protease Inhibitor Cocktail (Roche)) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were pelleted, resuspended in HSB (PBS supplemented with 150 mM NaCl, 1 mM DTT, protease inhibitor cocktail Complete (Roche)) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed once with PBS and resuspend with Tris-HCl containing 4 mM EDTA and 1× complete protease inhibitor cocktail (Roche). Dounce homogenizer is used to fractionate the cell membranes and then 3 min 600 g centrifugation was used to obtain Post-Nuclear Supernatants (PNS) ...
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bioRxiv - Molecular Biology 2023Quote: ... for the detection of the protein A fragment of the TAP tag or anti-HA peroxidase 1/500 (clone 3F10, Roche).
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bioRxiv - Immunology 2022Quote: ... Following 79 μL of 50 mM Tris-HCl was added together with 1 μL of 100x protease inhibitor cocktail (Roche) before the probe sonicated with VibraCell probe (Sonics & Materials ...
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bioRxiv - Molecular Biology 2023Quote: Thawed and washed cell pellets were lysed in 1 mL sterile PBS with 10% (v/v) glycerol and 1x cOmplete mini protease inhibitor cocktail (Roche). Ten micrograms of protein were separated by SDS-PAGE and transferred to a nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... resuspended in 1 ml of the buffer required for each particular application (see below) and supplemented with protease inhibitors (Roche), and then disrupted by sonication ...
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bioRxiv - Neuroscience 2023Quote: ... and then incubated in an anti-digoxigenin antibody (alkaline-phosphatase-coupled anti-digoxigenin; RRID:AB_514497, diluted 1:3,500; Roche Diagnostics; Table 2) for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Five volumes of 50 mM Tris pH 8 was added and proteins were digested over night with trypsin (1:50) (Roche) at 37°C while shaking at 800 rpm ...
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bioRxiv - Immunology 2023Quote: Spleens were injected with HBSS (with Ca2+ and Mg2+) containing 1 mg/mL of collagenase D and 10 μg/mL of DNase I (Roche) and incubated for 20 minutes at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... and the cell pellet was resuspended in 1 ml hypotonic buffer supplemented with 10 µg/ml PMSF and protease inhibitor cocktail (Roche). Cells were incubated on ice for 10 min ...
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bioRxiv - Cell Biology 2023Quote: 293T cells to express FLAG-tagged human RSPH proteins transiently were lysed with 1% Triton X-100 in 1X HEPES buffer with EDTA-free protease inhibitor cocktail (Roche) by rocking at 4 °C for 2 hours ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... kidney (human primary renal proximal tubule epithelial cells, RPTEC), and liver (human hepatocellular carcinoma, HepG2) [45–50] cells were assessed using a standard WST-1 (Roche) cell viability assay ...
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bioRxiv - Biochemistry 2023Quote: ... SRA01/04 cell lysates were obtained using RIPA lysis buffer (Beyotime) supplemented immediately before use with 10 μl ml-1 phosphatase and protease inhibitors (PhosSTOPTM, Roche). The bicinchoninic acid assay (Beyotime ...
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bioRxiv - Immunology 2023Quote: ... tumor tissue was dissected into approximately 1– 5 mm3 fragments and digested with collagenase Type D (2 mg/ml; Roche) and DNase I (1 mg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... 1% Triton X-100, 5 mM EGTA, 1 mM DTT, 2 mM MgCl, and one EDTA-free protease inhibitor cocktail tablet; Roche) and sonicated in an ice bath for 6 minutes ...
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bioRxiv - Microbiology 2023Quote: ... ChIP-seq libraries were prepared by Duke University Sequencing and Genomic Technologies Core Facility (Duke Sequencing Facility) from 1 ng of ChIP DNA using the KAPA HyperPrep Library Kit (Roche) to generate Illumina-compatible libraries ...
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bioRxiv - Cancer Biology 2023Quote: ... Thawed pellets were lysed in LB1 (50 mM HEPES-KOH pH 7.6, 140 mM NaCl, 1 mM EDTA, 10% Glycerol, 0.5% NP- 40, 0.25% Triton X-100, Roche protease inhibitor cocktail) for 20 min with rotation at 4°C and cleared by centrifugation at 300 x g for 5 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were lysed 10 min on ice in 1% (v/v) Triton X-100 in TBS supplemented with protease inhibitor (cOmplete, Roche) and phosphatase inhibitor (PhosStop ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5% Zwittergent 3-12 (N-dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate) and protease inhibitor (Complete, Roche Applied Science)) for 2 h at 0°C (ref A) ...
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bioRxiv - Developmental Biology 2023Quote: ... The total contents of Tie1-HA and Tie1KD-HA were assayed in a parallel run using anti-HA antibody (1:2000, Roche). As a loading control ...