Labshake search
Citations for Roche :
5701 - 5750 of 7579 citations for 2 1H Imidazol 1 yl 6 methyl 4 pyrimidinemethanamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... 1% NP-40 complemented with PIC (Complete EDTA-free Proteinase inhibitor cocktail tablets (11836170001, Roche). The lysates were incubated for 1 hour at 4°C with rotation and then centrifuged for 20 min at 16300 g at 4°C ...
-
bioRxiv - Cell Biology 2024Quote: ... 10% [v/v] glycerol) supplemented with 1× cOmplete EDTA-free protease inhibitor cocktail tablets (Roche). The resuspended cells were incubated on ice for 30 min with 1 mg/mL egg lysozyme and sonicated (50% amplitude ...
-
bioRxiv - Plant Biology 2024Quote: ... the non-specific sites were blocked with 1% Blocking Reagent (Roche, Cat No./ID: 11096176001). The slides were incubated with Anti-Digoxigenin-AP Fab fragment (Roche ...
-
bioRxiv - Pathology 2024Quote: ... the DRGs are incubated at 37°C with 1 mg/mL collagenase A (10103578001, Roche) for 15 minutes ...
-
bioRxiv - Biochemistry 2024Quote: ... briefly, cells were then resuspended in lysis buffer (50μg/ml lysozyme, 1×protease inhibitor(Roche), 250 U/ml benzonase ...
-
bioRxiv - Biochemistry 2024Quote: ... Proteolysis was run overnight at 37°C with 1:50 (w/w) recombinant trypsin (Roche). The peptides obtained were fractionated in a 44-min chromatographic run (Evosep One ...
-
bioRxiv - Developmental Biology 2024Quote: ... Kidneys were incubated in alkaline phosphatase-conjugated anti-DIG antibody (1:4000, Roche, Cat#11093274910). For in situ detection ...
-
bioRxiv - Microbiology 2024Quote: ... Antibodies used in this study for immunoblot detection were rat anti-HA (1:1000, Roche), mouse anti-myc (1:100 ...
-
bioRxiv - Immunology 2024Quote: ... followed by staining of CD3 and PD-1 w with a BenchMark Ultra autostainer (Roche). Tim-3 was stained manually after heat-induced epitope retrieval using the “high pressure” program on an electric pressure cooker (Cuisinart CPC-600 ...
-
bioRxiv - Neuroscience 2024Quote: ... Cells were then permeabilized in 1 % bovine serum albumin (BSA; Roche Diagnostics GmbH, Germany, 10735078001) in PBS-Triton 0.1 % solution for 5 min and blocked in 1 % BSA in PBS at room temperature for 1 h ...
-
bioRxiv - Neuroscience 2024Quote: ... were used to identify dendrites and anti-DIG to recognize probes (1:1000, Roche, 11207733910). TSA Plus Fluorescein Systems from PerkinElmer (TSA plus Cyanine detection kit ...
-
bioRxiv - Neuroscience 2024Quote: Cells were harvested using a lysis buffer (TBS with 1% Triton X-100, 1x Roche EDTA free cOmplete protease inhibitor ...
-
bioRxiv - Immunology 2024Quote: ... Cells were then washed twice with Buffer 1 (1X eBioscience Perm/Wash Buffer, 1X Roche cOmplete EDTA-free Protease Inhibitor ...
-
bioRxiv - Cell Biology 2024Quote: ... 0.5% Triton X-100 and 1 EDTA-free protease inhibitor cocktail tablet (Roche: Cat#05056489001) per 50 mL buffer ...
-
bioRxiv - Neuroscience 2024Quote: ... pH 7.25 with 0.5M KOH with addition of 1 U/μl recombinant RNase inhibitor (Roche) immediately prior to experiment and final osmolarity of 315–320 mOsm ...
-
bioRxiv - Neuroscience 2024Quote: Cells were lysed with 200 µL lysis buffer (1 % SDS + protease inhibitor cocktail tablet Roche) per well in 6-well plates and the lysate was collected by scraping ...
-
bioRxiv - Genomics 2024Quote: ... 0.5% N-Lauroylsarcosine) and resuspended in 100 μl of complete LB3 (LB3 containing 1 × Roche cOmplete Mini Protease Inhibitor Cocktail (Roche ...
-
bioRxiv - Bioengineering 2024Quote: ... resuspended into 1 mL of the same buffer supplemented with cOmplete™ protease inhibitor (Roche) and moved into a 2 mL screw-cap tube with 400 μL of 0.5 mm diameter glass beads ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cells/collagen discs were digested in HBSS adding 1:50 10mg/ml Collagenase P (Roche) for ∼30 minutes ...
-
bioRxiv - Cell Biology 2024Quote: ... 50 mM NaF and 1 mM β- mercaptoethanol) supplemented with protease inhibitor cocktail (Roche, 11836170001). Immunoprecipitations were performed using the indicated primary antibody and Dynabeads™ protein G beads (10004D ...
-
bioRxiv - Cell Biology 2024Quote: ... Embryos were dechorionated prior to metronidazole (MTZ) ablation using 1 mg/mL pronase (Roche, 165921). Starting at 2 dpf ...
-
bioRxiv - Cell Biology 2024Quote: ... and then overnight at 37°C in 1 mg/ml collagenase P (Roche, Basel, Switzerland) prepared in 10%CM (same composition as above ...
-
bioRxiv - Cell Biology 2024Quote: ... Primary antibodies used in this study: mouse monoclonal anti-GFP at 1:250 (Roche, 1814460), chicken polyclonal anti-GFP 1:40 (Abcam Ab13970) ...
-
bioRxiv - Developmental Biology 2024Quote: ... Then they were incubated with Anti-Digoxigenin alkaline phosphatase antibody (1:5000) (Roche, Cat. 11093274910) overnight at 4°C following by BM Purple (Roche ...
-
bioRxiv - Developmental Biology 2024Quote: The following antibodies and staining reagents were used: rat anti-HA (1:500; Roche, 3F10), mouse anti-Calnexin99A (1:10 ...
-
bioRxiv - Cancer Biology 2021Quote: ... tissues were homogenized in EBC-lysis buffer containing 1 mM Complete protease inhibitors cocktail (Roche Diagnostics) and 1 mM protein phosphatase Inhibitor Cocktail I (Sigma Aldrich) ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Cultures were pelleted at 1500 g and resuspended in 1 ml Buffer A (15 mM Tris pH 7.5, 80 mM KCl, 0.1 mM EGTA, 0.2 mM spermine, 0.5 mM spermidine, 1 × Roche cOmplete EDTA-free mini protease inhibitors ...
-
bioRxiv - Biochemistry 2021Quote: ... 25 mM imidazole) supplemented with 1 mM PMSF and one tablet of complete EDTA free (Roche). After sonication at 4°C ...
-
bioRxiv - Cell Biology 2020Quote: ... Blots were re-probed using rat anti-HA (clone 3F10, Roche cat #11867423001; 1:10,000 dilution) to detect ZMPSTE24 and visualized using goat anti-rat IRDye 680RD.
-
bioRxiv - Cell Biology 2020Quote: ... 1 μg RNA samples were reverse transcribed using a Transcriptor First Strand cDNA Synthesis Kit (Roche). RT-PCR was performed using a CFX Connect Real-Time PCR Detection system (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Samples were then treated with DNase-free RNase (Roche; 5 μg.ml-1; 37°C; 30 min) and proteinase K (250 μg.ml-1 ...
-
bioRxiv - Molecular Biology 2021Quote: ... The samples were re-suspended in PBS containing 50 μg ml-1 RNase A (Roche Diagnostics) for 30 minutes at 4°C and stained with 50 μg ml-1 PI (Sigma) ...
-
bioRxiv - Developmental Biology 2021Quote: ... then incubated in the same buffer for 3h with anti-DIG-peroxidase antibodies (1:500, Roche) and revealed using the TSA-Plus Cyanine-3 kit (Perkin-Elmer) ...
-
bioRxiv - Developmental Biology 2020Quote: ... for 30 min at 37°C and additionally with 1 mg/ml of collagenase solution (Roche) for 12-18 hr at 37°C with agitation ...
-
bioRxiv - Developmental Biology 2020Quote: ... and sequential incubation with an anti-GFP primary antibody (mouse anti-GFP, 1:200, 11814460001, Roche) and a corresponding secondary antibody (goat anti-mouse Alexa Fluor 488) ...
-
bioRxiv - Developmental Biology 2022Quote: ... Total RNA (1 µg) was reversed transcribed with the Transcriptor First Strand cDNA Synthesis kit (Roche). Fragments of fz3 and wg were amplified and used for the templates for synthesizing antisense probes ...
-
bioRxiv - Neuroscience 2021Quote: Cell lysates in 1% sarcosyl were digested with 75 µg/ml proteinase K (PK; Roche, Switzerland) for 1 h at 37 °C and subsequently denatured in an equal volume of 2x sample loading buffer at 110 °C ...
-
bioRxiv - Neuroscience 2021Quote: ... Sections were washed in PBS and the nuclei were counterstained with DAPI(1 μg/ml, Roche Diagnostics ...
-
bioRxiv - Molecular Biology 2021Quote: ... 0.5 mM TCEP] supplemented with 1 mM PMSF and 1X cOmplete EDTA-free protease inhibitor (Roche). Sonication was performed on ice for 5 cycles with 1 s on ...
-
bioRxiv - Molecular Biology 2020Quote: ... the membrane was blocked for 1 hour at room temperature by x1 blocking reagent (Roche, Germany) dissolved in TBS-T buffer ...
-
bioRxiv - Genetics 2021Quote: ... transferred on iBlot PVDF Membranes that were probed with anti-GFP (1:1000, Roche ref:11814460001) and custom-made anti-Sir3 (1:10000;(Ruault et al ...
-
bioRxiv - Developmental Biology 2020Quote: ... and then incubated in a solution of anti-Digoxigenin-POD Fab Fragments (Roche, Mannheim, 1:1000). The samples were rocked gently overnight at 4 □ ...
-
bioRxiv - Microbiology 2021Quote: Cellular proliferation was evaluated by the colorimetric test WST-1 according to the manufacturer’s instructions (Roche). Cellular viability was assessed by staining the cells with the LIVE/DEAD Fixable Near-IR Dead Cell Stain (Thermo Fisher ...
-
bioRxiv - Neuroscience 2020Quote: ... followed by incubation with 1:2000 dilution of anti-DIG-alkaline phosphatase-coupled antibody (Roche, Switzerland) in 10% sheep serum overnight ...
-
bioRxiv - Cancer Biology 2021Quote: ... superoxide dismutase activity was measured using a water-soluble tetrazolium salt (WST-1) (purchased from Roche) after drug treatment for 3 days ...
-
bioRxiv - Biophysics 2021Quote: ... The following primary antibodies were used at the indicated dilutions: mouse anti-Myc (Roche, 1:3000), goat anti-p75NTR (Neuromics ...
-
bioRxiv - Cancer Biology 2021Quote: ... Western blots were performed by standard methods using rat anti-HA (1:1000; clone 3F10, ROCHE), rabbit anti-GFP (1:2000 ...
-
bioRxiv - Biochemistry 2020Quote: ... 5% (v/v) glycerol and 1 mM TCEP containing an EDTA-free protease inhibitor tablet (Roche). The cell suspension was sonicated on ice and clarified by centrifugation at 27,000g for 15 min ...
-
bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with alkaline phosphatase (AP)-conjugated anti-DIG antibody (1:1000, 11093274910; Roche) at 4°C for two days ...
-
bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies were used in this study: mouse monoclonal anti-GFP (1:2’000; Roche), anti-HA (clone 16B12 ...