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Citations for Roche :
5601 - 5650 of 7615 citations for 7 Methylimidazo 1 2 a pyridine 3 carbaldehyde since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... cells were lysed in 1% Triton X-100 buffer with phosphatase and protease inhibitors (Roche). Protein was separated by 4-12% Bis-Tris protein gel (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... The cell proliferation was evaluated using the Cell Proliferation Reagent WST-1 (CELLPRO-RO, Roche).
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bioRxiv - Cancer Biology 2023Quote: ... by western blot with HRP-conjugated anti-HA antibody (clone 3F10 Roche 12013819001, 1:1,000); and POU5F1B downregulation upon shRNA transduction in LS1034 was analyzed by quantitative real-time PCR using P3 pair of primers ...
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bioRxiv - Biophysics 2023Quote: ... Cell Proliferation Reagent WST-1 and Cytotoxicity Detection KitPLUS (LDH) were obtained from Roche (Germany). 5× HOT FIREPol® EvaGreen® qPCR Supermix was acquired from Solis BioDyne (Estonia) ...
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bioRxiv - Cancer Biology 2023Quote: ... spun down and treated with 0.5-1 ml of RIPA buffer supplemented with protease (Roche) and phosphatase inhibitor cocktail II (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... Further enzymatic digestion was performed using 0.7 mg ml-1 of Collagenase (Collagenase A, Roche) in DMEM medium at 37 °C for 45 min ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM TCEP and supplemented with EDTA-free complete protease inhibitor tablets (Roche Applied Science). Cells were sonicated and then spun at 12000 rpm for 1h at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... and 1 mM DTT (TNMD buffer) supplemented with cOmplete EDTA-free protease inhibitor cocktail (Roche). The insoluble myrArf1•GTP was solubilized using 20 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2023Quote: ... DNA from 1% agarose gel was capillary transferred to a positively charged nylon membrane (Roche) and cross-linked by UV irradiation by standard procedures ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was then incubated with the primary antibody (anti-HA, Roche, 11583816001, 1:1000) in TBST + 5% non-fat dry milk overnight at 4 °C followed by incubation with secondary antibodies (Goat anti-Mouse IgG ...
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bioRxiv - Plant Biology 2024Quote: ... The following primary and secondary antibodies were used: α-HA (Roche 11867431001; 1: 10,000 dilution), α-Myc (Sigma-Aldrich C3956 ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 μl of extracted DNA was PCR amplified using KAPA HiFi HotStart ReadyMix (Roche 07958927001) and primers that generate a ∼750 bp amplicon surrounding the Hand2 translational start ...
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bioRxiv - Plant Biology 2024Quote: ... and then probed with anti-HA-peroxidase at a dilution of 1:2500 (Roche 12013819001) for 1 h at room temperature.
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bioRxiv - Cancer Biology 2024Quote: ... 0.1% SDS) supplemented with 1 mM DTT and 1X complete protease inhibitor cocktail (Roche, 11697498001). The cells were incubated on ice in lysis buffer for 30 mins before vortexing followed by centrifugation at 20,000 g for 15 mins at 4°C in a microcentrifuge to remove the cell debris ...
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bioRxiv - Microbiology 2024Quote: ... 1 mM β-mercaptoethanol) plus protease inhibitor (Complete Ultra Tablets, Mini, EDTA-free, EASYpack, Roche). Cells were then disrupted by French press cell lysis and centrifugated at 12000 rpm for 1 hour at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were incubated in blocking solution (0.2% Roche block, 10% FBS, 1% DMSO in PBST) for 30 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... 0.5% Triton X-100 and 1 EDTA-free protease inhibitor cocktail tablet (Roche: Cat#05056489001) per 50 mL buffer ...
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bioRxiv - Cell Biology 2024Quote: ... 10% [v/v] glycerol) supplemented with 1× cOmplete EDTA-free protease inhibitor cocktail tablets (Roche). The resuspended cells were incubated on ice for 30 min with 1 mg/mL egg lysozyme and sonicated (50% amplitude ...
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bioRxiv - Plant Biology 2024Quote: ... the non-specific sites were blocked with 1% Blocking Reagent (Roche, Cat No./ID: 11096176001). The slides were incubated with Anti-Digoxigenin-AP Fab fragment (Roche ...
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bioRxiv - Pathology 2024Quote: ... the DRGs are incubated at 37°C with 1 mg/mL collagenase A (10103578001, Roche) for 15 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... briefly, cells were then resuspended in lysis buffer (50μg/ml lysozyme, 1×protease inhibitor(Roche), 250 U/ml benzonase ...
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bioRxiv - Biochemistry 2024Quote: ... Proteolysis was run overnight at 37°C with 1:50 (w/w) recombinant trypsin (Roche). The peptides obtained were fractionated in a 44-min chromatographic run (Evosep One ...
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bioRxiv - Developmental Biology 2024Quote: ... Kidneys were incubated in alkaline phosphatase-conjugated anti-DIG antibody (1:4000, Roche, Cat#11093274910). For in situ detection ...
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bioRxiv - Microbiology 2024Quote: ... Antibodies used in this study for immunoblot detection were rat anti-HA (1:1000, Roche), mouse anti-myc (1:100 ...
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bioRxiv - Immunology 2024Quote: ... followed by staining of CD3 and PD-1 w with a BenchMark Ultra autostainer (Roche). Tim-3 was stained manually after heat-induced epitope retrieval using the “high pressure” program on an electric pressure cooker (Cuisinart CPC-600 ...
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bioRxiv - Cancer Biology 2021Quote: ... tissues were homogenized in EBC-lysis buffer containing 1 mM Complete protease inhibitors cocktail (Roche Diagnostics) and 1 mM protein phosphatase Inhibitor Cocktail I (Sigma Aldrich) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Cultures were pelleted at 1500 g and resuspended in 1 ml Buffer A (15 mM Tris pH 7.5, 80 mM KCl, 0.1 mM EGTA, 0.2 mM spermine, 0.5 mM spermidine, 1 × Roche cOmplete EDTA-free mini protease inhibitors ...
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bioRxiv - Biochemistry 2021Quote: ... 25 mM imidazole) supplemented with 1 mM PMSF and one tablet of complete EDTA free (Roche). After sonication at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Blots were re-probed using rat anti-HA (clone 3F10, Roche cat #11867423001; 1:10,000 dilution) to detect ZMPSTE24 and visualized using goat anti-rat IRDye 680RD.
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bioRxiv - Cell Biology 2020Quote: ... 1 μg RNA samples were reverse transcribed using a Transcriptor First Strand cDNA Synthesis Kit (Roche). RT-PCR was performed using a CFX Connect Real-Time PCR Detection system (Bio-Rad) ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were then treated with DNase-free RNase (Roche; 5 μg.ml-1; 37°C; 30 min) and proteinase K (250 μg.ml-1 ...
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bioRxiv - Molecular Biology 2021Quote: ... The samples were re-suspended in PBS containing 50 μg ml-1 RNase A (Roche Diagnostics) for 30 minutes at 4°C and stained with 50 μg ml-1 PI (Sigma) ...
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bioRxiv - Developmental Biology 2021Quote: ... then incubated in the same buffer for 3h with anti-DIG-peroxidase antibodies (1:500, Roche) and revealed using the TSA-Plus Cyanine-3 kit (Perkin-Elmer) ...
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bioRxiv - Developmental Biology 2020Quote: ... for 30 min at 37°C and additionally with 1 mg/ml of collagenase solution (Roche) for 12-18 hr at 37°C with agitation ...
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bioRxiv - Developmental Biology 2020Quote: ... and sequential incubation with an anti-GFP primary antibody (mouse anti-GFP, 1:200, 11814460001, Roche) and a corresponding secondary antibody (goat anti-mouse Alexa Fluor 488) ...
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bioRxiv - Developmental Biology 2022Quote: ... Total RNA (1 µg) was reversed transcribed with the Transcriptor First Strand cDNA Synthesis kit (Roche). Fragments of fz3 and wg were amplified and used for the templates for synthesizing antisense probes ...
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bioRxiv - Neuroscience 2021Quote: Cell lysates in 1% sarcosyl were digested with 75 µg/ml proteinase K (PK; Roche, Switzerland) for 1 h at 37 °C and subsequently denatured in an equal volume of 2x sample loading buffer at 110 °C ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed in PBS and the nuclei were counterstained with DAPI(1 μg/ml, Roche Diagnostics ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 mM TCEP] supplemented with 1 mM PMSF and 1X cOmplete EDTA-free protease inhibitor (Roche). Sonication was performed on ice for 5 cycles with 1 s on ...
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bioRxiv - Molecular Biology 2020Quote: ... the membrane was blocked for 1 hour at room temperature by x1 blocking reagent (Roche, Germany) dissolved in TBS-T buffer ...
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bioRxiv - Immunology 2019Quote: ... skin samples were digested for 1h at 37°C using 1 U/mL Liberase TM (Roche) and 5 µg/mL DNAse I (Sigma) ...
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bioRxiv - Microbiology 2019Quote: ... and adjusted to 1 x 108 tachyzoites/ml with PBS containing protease inhibitors (cOmplete mini, Roche). Parasites were lysed by five freeze-thaw cycles (liquid nitrogen/37°C) ...
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bioRxiv - Pathology 2019Quote: ... Lysis buffer mix consisting of 100 μL Lysis buffer (Vial 1, Roche FFPET Isolation Kit # 06650775001), 100 μL dH2O ...
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bioRxiv - Biochemistry 2019Quote: ... the membranes were incubated with anti-HA-peroxidase antibody (1:5,000; Roche, Cat# 12013819001, clone 3F10) for 1 h at room temperature followed by washing with PBS-T for three times ...
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bioRxiv - Plant Biology 2019Quote: ... Jas9-VENUS and ACTIN were detected using mouse monoclonal antibodies anti-GFP 1:1,000 (11814460001, Roche) or anti-actin 1:2,000 (A0480 ...
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bioRxiv - Genomics 2019Quote: ... We ethanol precipitated the flow through by adding 1 μl glycogen (20 μg/μl; Roche, #10901393001) and 2.5 volumes 100% ice cold ethanol followed by incubation at - 20 °C for at least 30 min and then 20 min of centrifugation at 15,000 x g and 4 °C ...
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bioRxiv - Immunology 2019Quote: Spleens were injected with 1 ml of PBS supplemented with Liberase TL (0.125 mg/ml, Roche) and DNAse I (100 μg/ml ...
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bioRxiv - Cancer Biology 2019Quote: ... and 1% Nonidet P-40) in the presence of a protease and phosphatase inhibitor cocktail (Roche) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... Western blot analysis was performed using peroxide-conjugated anti-hemaglutinin antibody at 1:250 dilution (Roche), anti-cMYC antibody 9E10 (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2019Quote: ... 0.5 μM of both forward and reverse primers and 1 × FastStart SYBR Green Master mix (Roche). Steady state levels of transcripts were quantified using primers listed in Supplemental Table 2 ...