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Citations for Roche :
5501 - 5550 of 8217 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... pH 7.4 with 1% Triton X100 and 1x Complete Mini Protease Inhibitor Mixture (Roche Applied Science, Indianapolis, IN). Cell lysate was cleared by centrifugation at 15,000x g for 10 min ...
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bioRxiv - Microbiology 2020Quote: Whole-cell extracts were obtained from liver tissue or cell pellets lysed in buffer (0.1%, sodium dodecyl sulfate, 0.1% sodium deoxycholate, 1% IGEPAL) supplemented with Protease and Phosphatase Inhibitor cocktails (Roche). Protein extracts were separated by SDS-polyacrylamide gel electrophoresis then transferred onto polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2021Quote: ... The membranes were blocked using 10% skimmed milk in PBS containing 0.1% Tween 20 (PBST) and then incubated with rat anti-HA high affinity (1:1,000; Roche) and rabbit anti-T ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was aspirated and the pellet was resuspended in RIPA buffer (1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS supplemented with Roche protease and phosphatase inhibitor tablet just before use) ...
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bioRxiv - Neuroscience 2020Quote: ... 1 M NaCl and protease inhibitor cocktail (cOmplete, Mini, Roche; two tablets per 10 mL of lysis buffer). The lysate was sonicated before AHA-labeled proteins were covalently coupled to alkyne-tagged agarose beads using reagents provided in the kit ...
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bioRxiv - Genomics 2020Quote: ... Cells were collected in lysis buffer (1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS and protease inhibitor (#11873580001, Roche) in PBS ...
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bioRxiv - Microbiology 2021Quote: ... 10 mM Tris, pH 7.5, 1 mM EDTA, 0.5% Nonidet P-40, protease and phosphatase inhibitor cocktail [Roche]). Cell lysates were denatured in Laemmli sample buffer and incubated for 5 min at 95°C ...
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bioRxiv - Molecular Biology 2020Quote: ... and washed three times with 1 mL digitonin buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 1x Roche complete protease inhibitors ...
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bioRxiv - Neuroscience 2021Quote: ... Fcgr1 mRNA signal was then detected using sheep anti-DIG antibody (1:500; Roche Diagnostics Corp., Indianapolis, IN) followed by the secondary antibody (Donkey anti-sheep IgG Alexa 488 ...
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bioRxiv - Immunology 2020Quote: ... 1 μM of each primer combination and 4 μL of Lightcycler FastStart DNA MasterPLUS SYBR Green I (Roche) in a total volume of 20 μL ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were collected in lysis buffer (1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS and protease inhibitor (#11873580001, Roche) in PBS)39 ...
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bioRxiv - Immunology 2020Quote: ... The eluate was then treated with RNaseA for 1 h at 37°C and with Proteinase K (Roche) for 1 h at 55°C and the DNA was recovered using a Qiagen PCR purification kit.
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bioRxiv - Microbiology 2021Quote: ... The supernatant was removed and the pellet was dissolved in 500 µl of ice-cold nuclear solubilization buffer (25 mM HEPES [pH 6.8], 1 M sorbitol, 0.5% NP-40, 250 μg/ml PMSF, cOmplete [EDTA-free protease inhibitor cocktail; Roche]). Following incubation for 30 min at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were lysed in lysis buffer (1.5M NaCl, 20 mM Phosphate buffer pH 7.5, 20 mM Imidazole, 10mg/mL lysozyme, 1 tablet of Roche EDTA-free protease inhibitor cocktail Millipore Sigma 11873580001 ...
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bioRxiv - Microbiology 2021Quote: ... and 0.1 mM CaCl2) with or without DNase I (final concentration 160 U mL-1, Roche, cat. # 04716728001) and were incubated at 37°C for 3 h ...
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bioRxiv - Genomics 2022Quote: ... The ConA bound nuclei were then incubated with primary antibody diluted 1:100 in Antibody Buffer (20 mM HEPES pH7.5, 150 mM NaCl, 0.5 mM spermidine, Roche Complete Protease Inhibitor Cocktail ...
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bioRxiv - Cancer Biology 2022Quote: ... For enteric glial cell experiments were performed by hypotonic lysis in 200 µl of buffer A (20 mM HEPES, pH 8.0, 1 mM DTT, 0.1% NP-40 and a proteinase inhibitor cocktail from Roche). The cell lysates were incubated on ice for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... Fab fragments diluted 1:10,000 in the blocking buffer for 30 minutes at room temperature (Roche, Cat#11093274910) following the manufacturer’s protocols ...
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bioRxiv - Microbiology 2022Quote: ... were incubated at 4°C with hypotonic lysis buffer (10 mM Tris–HCl pH 8.0, 1 mM KCl, 1.5 mM MgCl2, with protease inhibitors (ROCHE)) for 2 hours while rotating ...
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bioRxiv - Plant Biology 2022Quote: ... Cell pellets were thawed on ice and resuspended in lysis buffer (20 mM sodium phosphate pH 6.8, 10 mM DTT, 1 mM EDTA, and 0.25× protease inhibitor cocktail [Roche]). Cells were sonicated on ice and centrifuged for 10 min at 500 g and 4 °C to remove intact cells and cell debris ...
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bioRxiv - Epidemiology 2020Quote: ... Real-time PCR assays were performed with LightCycler® 480 Probe Master Mix 1× (Roche Applied Science, Germany), using 200 nM of primers and probes in a final volume of 12 µl and 2 µl of control DNA were added ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Whole cell extracts were prepared in HEPES whole cell lysis buffer (20 mM HEPES, pH 7.4, 1% TX-100,1x phosphoSTOP complete inhibition cocktail tablets [Roche] ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated for 1 h with rat anti-HA high affinity monoclonal antibodies (Roche Applied Science, Penzberg, Germany) at 1:500 ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in 1ml of cold Lysis Buffer 1 (10mM Tris pH 8.0, 10mM NaCl, 0.2% IGE-PAL, Protease inhibitor (Roche)) and incubated on ice for 15 minutes ...
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bioRxiv - Physiology 2020Quote: ... and 1 mM phenylmethylsulfonyl fluoride (PMSF)) supplemented with EDTA-free protease inhibitor cocktail and phosphatase inhibitors cocktail (Roche). Tissue lysates were centrifuged at 14,000 rpm for 20 min at 4°C twice before using the supernatant for western blot studies ...
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bioRxiv - Developmental Biology 2021Quote: ... and incubated overnight at 4 °C with alkaline phosphatase–conjugated anti-digoxigenin antibodies in TBTX (1:2000; Roche). Following extensive washing in TBTX ...
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bioRxiv - Microbiology 2021Quote: ... Plasma viral load in ART-treated PLWH was measured using the Amplicor HIV-1 monitor ultrasensitive method (Roche).
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bioRxiv - Molecular Biology 2020Quote: ... 2.5 mM MgCl2) supplemented with 0.5% Triton X-100 and 1 pill Complete® protease inhibitor cocktail (Roche). The cell lysates were subjected to two 5 sec pulses of sonication at the lowest settings (Branson Sonifier 250 ...
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bioRxiv - Microbiology 2021Quote: ... and polymerized by UV light prior to labeling with anti-HA antibody (Roche, clone 3F10, 1:40 dilution) and 10nm gold bead conjugated goat anti-rat (Electron Microscopy Sciences ...
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bioRxiv - Genetics 2020Quote: ... then resuspended in 600 µl of ChIP lysis buffer (50mM HEPES, pH 7.5, 140mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, one tablet Roche cOmplete mini EDTA-free Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells are pelleted and resuspended in 1 mL of Lysis Buffer (200 mM NaCl, 25 mM HEPES, 1 mM MgCl2, 0.2 mM EDTA, 0.5% NP-40, 1X Roche complete inhibitor ...
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bioRxiv - Molecular Biology 2021Quote: Lysates for Western-blot were made in a 1% triton buffer including cOmplete protease inhibitor cocktail (Roche 11697498001) and Western-Blot and flow cytometric staining was performed as described previously (41),(42) ...
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bioRxiv - Microbiology 2020Quote: ... 0.05 μL of 20 μg/μL (1 μg BSA/reaction) of nonacetylated bovine serum albumin (BSA, 10711454001, Roche), 1 μL SuperScript™ III RT/Platinum Taq Mix and 1.85 μL of primer/probe mix (2019-nCoV CDC EUA Kit ...
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bioRxiv - Microbiology 2021Quote: ... A 1 uL aliquot was used to amplify the V1-V3 region using HiFi HotStart ReadyMix (KAPA BioSystems) and the Illumina PCR protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Detection of hybridized probes was performed with anti-DIG antibodies AP fragments (Roche, Basel Switzerland; dilution 1:1000) overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... and lysed by resuspension in lysis buffer (150 mM NaCl, 50 mM Tris-HCl pH 7.4, 1 mM EDTA, 0.5% NP-40, and protease inhibitor [Roche]) with rotation at 4°C for 30 min ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell proliferation was assessed by WST-1 assay as per manufacturer’s protocol (Roche Applied Science, Indianapolis, IN, USA). Colony formation assay was performed with MethoCult methylcellulose-based media as per manufacturer’s protocol (Stemcell Technologies ...
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bioRxiv - Genetics 2020Quote: ... 200 μl lysis buffer (8M Urea, 1% SDS, 50 mM Tris pH 8.5, Roche protease and phosphatase inhibitor) were added to the pellet and samples were vortexed and sonicated on ice ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM benzamidine) supplemented with 0.1 mg/mL lysozyme and one Complete EDTA-free protease inhibitor tablet (Roche) per 100 mL ...
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bioRxiv - Genomics 2021Quote: ... 250 nL of protease mix was added (1× NEB CutSmart buffer, 1.0 mg/mL Proteinase K (Roche, 000000003115836001)) ...
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bioRxiv - Biochemistry 2020Quote: ... 250 mM imidazole) and incubated after addition of 1 or 10 mM phenylmethanesulfonyl fluoride (PMSF) (Roche, Basel, Switzerland), or 1 mM H2O2 (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: Cell proliferation in LNCaP IL1B overexpression and vector cells was assessed using Cell Proliferation Reagent WST-1 (Roche), per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 10 ml of DMEM media and 1 mg/ml collagenase D (Sigma-Aldrich, COLLD-RO Roche, #11088866001). The tubes were placed on a gentleMACS Dissociator (MACS Miltenyi Biotec ...
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bioRxiv - Microbiology 2020Quote: ... qPCR was carried out using LightCycler® 480 SYBR Green 1 Master reaction mix (Roche Diagnostics, Mannheim, Germany) following the manufacturer’s temperature program with 62°C as the primer hybridization and elongation temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were lysed in lysis buffer (1.5M NaCl, 20 mM Phosphate buffer pH 7.5, 20 mM Imidazole, 10mg/mL lysozyme, 1 tablet of Roche EDTA-free protease inhibitor cocktail Millipore Sigma 11873580001 ...
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bioRxiv - Immunology 2021Quote: ... minced thymuses from fetuses and postnatal mice were digested with 0.5 and 1 unit/ml Liberase TM (Roche) in the presence of 0.01% DNase I (Roche) ...
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bioRxiv - Developmental Biology 2021Quote: ... wild-type or transgenic animals in an equal volume of Pedro’s buffer (30mM HEPES, pH 7.5, 100mM KOac, 10mM EDTA, 10% glycerol, 1 mM DTT, 1x Roche complete mini protease inhibitors ...
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bioRxiv - Immunology 2021Quote: ... and digested for 20 min at 37 °C in medium supplemented with 1 mg/mL Collagenase D (Roche) and 2000 U/mL DNase I (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... In situ hybridization signals were detected with sheep anti-digoxigenin-AP Fab fragments (1:10000; Roche Diagnostics, Germany) conjugated with alkaline phosphatase ...
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bioRxiv - Cell Biology 2023Quote: ... 1% Triton X-100) complemented with final concentration of 1X EDTA-free Complete protease inhibitor cocktail (34044100, Roche) and 1 mM PMSF (78830 ...