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Citations for Roche :
5451 - 5500 of 8781 citations for 6H Imidazo 4 5 e 2 1 3 benzothiadiazole 7 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... 1 mM EDTA) containing 2x Complete protease inhibitor (Roche). Cleared cell lysates were incubated with Streptavidin beads (Pierce ...
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bioRxiv - Biochemistry 2024Quote: ... with 1 mini EDTA-free protease inhibitor tablet (Roche) and 1mM PMSF added per 10mL buffer used ...
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bioRxiv - Neuroscience 2024Quote: ... 1 mM PMSF and 1x Protease Inhibitor Cocktail (Roche), 100 µg/µl RNaseA) ...
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bioRxiv - Neuroscience 2024Quote: ... and 1 µg/ml cOmpleteTM protease inhibitor mixture (Roche). After incubation on ice for 15 min ...
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bioRxiv - Neuroscience 2024Quote: ... 1× cOmplete protease inhibitor cocktail (Roche, Indianapolis, IN, USA), and 0.25% sodium deoxycholate) ...
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bioRxiv - Neuroscience 2024Quote: ... permeabilized and blocked with 1% blocking reagent (Roche 11096176001), 0.5% BSA ...
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bioRxiv - Plant Biology 2024Quote: ... 1 mM PMSF) supplemented with Protease inhibitor cocktail (Roche) and 100 U/ml Ribolock (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2024Quote: ... Nuclei were stained with DAPI (1:500, Roche, 10236276001) and cytoplasm were stained with CellMask Orange (1:5000 ...
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bioRxiv - Immunology 2024Quote: ... and 1 U/ml dispase II (Roche Applied Science) in DMEM and placed on a 100 µm cell strainer ...
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bioRxiv - Cell Biology 2024Quote: ... and 1 μg of nonacetylated bovine serum albumin (Roche). All oligonucleotides were synthesized and provided by Tib-Molbiol (Berlin ...
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bioRxiv - Microbiology 2024Quote: ... 10μl /well cell proliferation reagent WST-1 (Roche 05015944001) was added ...
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bioRxiv - Neuroscience 2024Quote: ... 1% Triton X-100 supplemented with protease inhibitors (Roche). Media was removed from each well and replaced with STEN buffer ...
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bioRxiv - Developmental Biology 2024Quote: ... Incubation with anti-FLUOAP antibody (1:4000, Roche-1142638910) was done at 4°C overnight ...
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bioRxiv - Physiology 2024Quote: ... 1% Triton X100 and a protease inhibitor mixture (Roche, cOmplete™ ...
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bioRxiv - Pathology 2024Quote: ... 1% Triton X-100 with phosphatase inhibitor cocktail (Roche), pH 7.4 ...
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bioRxiv - Cell Biology 2024Quote: ... 1 tablet of protease inhibitors (cOmplete EDTA-free, Roche). The cell extract was clarified by centrifugation at 30,000 rpm in a TLA.103 rotor (Beckman ...
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bioRxiv - Cell Biology 2024Quote: ... 1% IGEPAL CA-630 and protease inhibitor cocktail (Roche)] was added and cells scraped into individual 1.5mL tubes ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 × Complete EDTA-free protease inhibitor (Merck Millipore/Roche), 1 × PhosSTOP (Merck Millipore/Roche)] and homogenized with a Dounce homogenizer on ice ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 × Complete EDTA-free protease inhibitor (Merck Millipore/Roche), 1 × PhosSTOP (Merck Millipore/Roche)] and incubated on ice for 20 min ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-HA antibody (3F10; Merck Millipore/Roche, 1/300), anti-Siwi antibody (1/400) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 × Complete EDTA-free protease inhibitor (Merck Millipore/Roche), 1 × PhosSTOP (Merck Millipore/Roche)] and homogenized with a Dounce homogenizer ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 mM TCEP (supplemented with protease inhibitor cocktail (Roche)) ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-HA (12CA5 mouse monoclonal antibody, Roche, 1:10.000), anti-Ubiquitin (P4D1 mouse monoclonal antibody ...
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bioRxiv - Neuroscience 2024Quote: ... 1% Triton X-100) with protease inhibitor (Roche, 6127000) and homogenized using a Dounce homogenizer ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 x EDTA free proteinase inhibitor (Roche, Indianapolis, IN)) and incubated for 30 minutes on ice ...
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bioRxiv - Developmental Biology 2019Quote: ... 100 µl 10% SDS, 500 µl 20% N-Lavroyl sarsosine sodium, 2 tablets of cOmplete ULTRA Protease Inhibitor Cocktail [Roche Cat.# 05892970001] in 10ml FA buffer). The sample was then sonicated using a Covaris S220 at the following settings ...
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bioRxiv - Developmental Biology 2022Quote: ... 100 µl 10% SDS, 500 µl 20% N-Lauroyl sarsosine sodium, 2 tablets of cOmplete ULTRA Protease Inhibitor Cocktail [Roche Cat.# 05892970001] in 10ml FA buffer). For each IP experiment ...
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bioRxiv - Cell Biology 2020Quote: ... A 2ml Dounce homogenizer was used to individually homogenize each brain in a 1:1 solution of Lysis-R:2X-RIPA buffer solution with protease inhibitors (Roche cOmplete tablets #11836153001). Each sample was sonicated three times for 7 seconds and then centrifuged at 5000g for 5min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Cells were harvested and resuspended in binding buffer (100 mM Tris- Cl pH 8.0, 150 mM NaCl, 1 mM EDTA, complete protease inhibitor cocktail (Roche; 1 tablet/10 mL) before sonicating on ice ...
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bioRxiv - Biochemistry 2021Quote: Bacteria were resuspended in 150 μL lysis buffer (1% Triton X100, 0.5% SDS, 1 tablet cOmplete EDTA-free protease inhibitor cocktail (Roche Diagnostics GmbH, Mannheim, Germany) in 10 mL PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... and sequentially incubated for 1 hour in a serum-based blocking solution and then for 1 hour in blocking solution containing the anti-digoxigenin (Roche, 11093274910; 1/2000) or anti-Fluorescein (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... The final pellet containing the chromatin fraction was resuspended in lysis buffer (10 mM HEPES, 500 mM NaCl, 1 mM EDTA, 1% NP-40, supplemented with protease and phosphatase inhibitor cocktails, Roche, and Benzonase, Millipore), sonicated 3 times at low amplitude ...
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bioRxiv - Molecular Biology 2020Quote: ... 10% Glycerol, 1 % Triton-X-100, 1.5 mM MgCl2, 1 mM EGTA, 10 mM Pyrophosphate, 100 mM NaF, Roche protease inhibitor cocktail 1x), and protein level quantified using the Pierce BCA Assay Kit (Thermo Scientific ...
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bioRxiv - Biophysics 2021Quote: ... the cells were washed with cold 1X-PBS prior to collection with 800μL of chilled lysis buffer (50mM TRIS-HCl [pH=7.4]; 150mM NaCl; 1mM EDTA; 1mM CaCl2; 1mM MgCl2; 1% Triton, 0.5% sodium deoxycholate; protease inhibitor cocktail [Roche] 1 tablet/10mL lysis buffer). The cells were incubated on ice for 30 minutes and then centrifuged at 13,000 rpm/ 15minutes/ 4° C ...
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bioRxiv - Microbiology 2020Quote: NUP153/NSP1-expressing cell pellets were resuspended in lysis buffer (1× PBS, 0.5 mM TCEP, 0.1 mM PMSF, 1× Roche cOmplete protease inhibitors, pH 7.4), and lysed in a homogenizer ...
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bioRxiv - Genetics 2019Quote: ... Sheered chromatin samples were pre-cleared by incubating with the 250ul aliquot of Dynabeads resuspended in 2ml of Dilution Buffer (20mM Tris pH=8, 2mM EDTA, 150mM NaCl, 1% Triton X-100, 1:50 Roche cOmplete protease inhibitor) for 1 hour at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... 10 mM NaCl, 1.5 mM MgCl2, 1 mM DTT, 100 μg/ml PMSF, 1 μg/ml pepstatin, and Roche Complete Protease Inhibitor Cocktail) with 100 U/ml of OmniCleave nuclease (Epicentre ...
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bioRxiv - Microbiology 2024Quote: ... 1.5 mM MgCl2, 0.1% Triton X-100, 1 mM DTT, 100 μg/ml PMSF, 1 μg/ml pepstatin, and Roche Complete Protease Inhibitor Cocktail), incubated for 20 minutes on ice ...
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bioRxiv - Microbiology 2024Quote: ... 1.5 mM MgCl2, 0.1% IGEPAL Nonidet P-40, 1 mM DTT, 100 μg/ml PMSF, 1 μg/ml pepstatin, and Roche Complete Protease Inhibitor Cocktail) and transferred to a Dounce homogenizer and homogenized with 20 slow strokes ...
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bioRxiv - Cell Biology 2024Quote: ... Beads were spun down and washed twice in 1 mL of buffer (50 mM Hepes, pH 7.4, 150 mM NaCl, 1 mM DTT, cOmplete (La Roche Ltd., Basel, Switzerland, 05056489001)) and subjected to sample preparation for MS measurement ...
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bioRxiv - Neuroscience 2023Quote: ... 1 mM EDTA, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, protease inhibitor cocktail [Roche], and PhosStop [Roche]; pH 7.5) and sonicated for 30 s ...
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bioRxiv - Neuroscience 2023Quote: ... The slices were lysed in lysis buffer (50 mM Tris/HCl, 1 mM EDTA, 1% SDS, phosphatase inhibitor cocktail (Roche, Indianapolis, IN, USA), and protease inhibitor cocktail (Roche) ...
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bioRxiv - Microbiology 2023Quote: ... and resuspended in 1 mL of buffer 1 (20 mM K-HEPES pH 7.9, 50 mM KCl, 10% glycerol, and Roche EDTA-free protease inhibitors). Subsequently ...
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bioRxiv - Biophysics 2023Quote: ... 1% Triton X-100, 10% glycerol, 10 mM NaH2PO4, 10 mM NaP2O7, 100 μM ZnCl2, 10 mM NaF, 1 Roche protease inhibitor tablet), lysed by sonication and the supernatant isolated by centrifugation ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 M NaCl, 30 mM imidazole, 1 mM DTT, 1 mM benzamidine, and one EDTA-free complete protease inhibitor cocktail tablet [Roche] per 50 ml). The lysate was clarified by centrifugation at 38,000 g in a fixed-angle rotor ...
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bioRxiv - Cell Biology 2024Quote: ... pH 8, 150 mm NaCl, 1 mm EDTA, 1% Triton X-100, and cOmplete™ EDTA-free Protease Inhibitor Mixture, Roche Applied Science) before clarification by centrifugation and enrichment for FLAG-tagged proteins using anti-FLAG® M2 Affinity Gel (A2220 ...
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bioRxiv - Cell Biology 2024Quote: ... followed by 1 hour incubation with the following primary antibodies diluted in blocking solution: mouse IgG1 mAb α-GFP (1:500, Roche Diagnostics #11814460001), mouse IgG2 mAb α-Centrin clone 20H5 (1:500 ...
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bioRxiv - Molecular Biology 2021Quote: ... 150 mM NaCl, 1 mM EDTA, 1% v/v Triton X-100, 10% v/v glycerol, 1x cOmplete protease inhibitor cocktail [Roche], and 1x PhosSTOP [Roche]). Cells were scraped off ...
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bioRxiv - Developmental Biology 2020Quote: ... expressing HA-tagged TWIST1 interaction candidates were thawed in 300 μL hypotonic lysis buffer (HEPES 20 mM, MgCl2 1 mM, Glycerol 10%, Triton 0.5 %. DTT 1 mM, 1x Complete protease inhibitor [Roche], Benzo nuclease 0.5 μl/ ml) and incubated at room temperature for 15 mins (for nuclease activity) ...
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bioRxiv - Genetics 2020Quote: ... 0.1 M ammonium bicarbonate, pH 8, 150 mM NaCl, 1 Roche mini protease inhibitor tablet without EDTA/10 ml, ½ Roche phosSTOP tablet/10 ml) using 14 × 1.5 min bursts on a BioSpec mini bead-beater at room temperature ...