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Citations for Roche :
501 - 550 of 883 citations for VEGF C Human 196a.a HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and the right inferior lung lobes were digested at 37°C with 630 µg/mL collagenase D (Roche) and 75 U/mL of DNase I (Sigma–Aldrich ...
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bioRxiv - Immunology 2022Quote: ... Skin samples were excised and incubated at 37°C for 90 min in dispase (2.5 mg/ml; Roche) or in liberase (0.25 mg/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... and incubated overnight at 4 °C with alkaline phosphatase–conjugated anti-digoxigenin antibodies in TBTX (1:2000; Roche). Following extensive washing in TBTX ...
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bioRxiv - Cancer Biology 2021Quote: ... and digested for 30 minutes at 37 °C in DMEM + 2 mg/mL Collagenase A (Roche, Basel, Switzerland) + DNase (Roche ...
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bioRxiv - Genetics 2019Quote: ... Samples were then incubated at 55 °C for 1h after addition of 40 μg of Proteinase K (Roche) and ...
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bioRxiv - Genomics 2019Quote: ... Probes were bound to 2 µg nuclear extracts pre-incubated with 1 µg poly d(I-C) (Roche) or 100-750ng YY1 full-length recombinant protein (31332 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... followed by a 20-min incubation at 37°C in CSS containing 1.5 mg/ml Collagenase D (Roche), 0.6 mM EDTA ...
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bioRxiv - Plant Biology 2019Quote: ... and incubated with anti-c-Myc primary antibody solution (GenScript, 1: 2,000 dilution in 1×TBS-T with 0.2% Blocking Reagent [Roche]) overnight at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... triglycerides and high-density lipoprotein cholesterol (HDL-c) concentrations using a Cobas C111 analyzer (Roche Diagnostics, Basilea, Sw). The intra- and inter-assay variation coefficients were less 5% in all determinations.
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bioRxiv - Evolutionary Biology 2020Quote: ... at 10 µg/ml for 40 minutes at 37° C before applying the TUNEL reaction mix (Roche Inc.) for 90 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... was used to remove excess antibody and amplified C-circles were detected with CDP-Star® kit (Roche). Membranes were imaged with the Odyssey® Fc Imager (LI-COR ...
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bioRxiv - Immunology 2021Quote: ... and digested for 20 min at 37 °C in medium supplemented with 1 mg/mL Collagenase D (Roche) and 2000 U/mL DNase I (Sigma-Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... they were digested for 30 minutes at 37°C in PBS 1X containing 1mg/mL Collagenase D (Roche), 100 U/mL DNAse I (Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: Primary adipocytes were isolated from epi-WAT after digested at 37°C for 1 hr with collagenase (Roche) in KRB supplemented with 3 mM glucose and 1% FA-free BSA ...
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bioRxiv - Neuroscience 2022Quote: ... and fixed at 50°C for 1 h before treatment with 1 ug/ml proteinase K (Roche; 3115828001) in lysis buffer (50 mM Tris HCl pH 8 ...
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bioRxiv - Neuroscience 2022Quote: ... at 95°C for 60 minutes or with a medium concentration of protease (Protease 1; Roche Tissue Diagnostics) for 4 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... and hybridized overnight at 40°C with digoxigenin (DIG)-labeled DNA probes in DIG Easy Hyb solution (Roche). After low stringency washes (washing twice with 2× SSC/0.1% SDS at room temperature ...
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bioRxiv - Genomics 2023Quote: ... Supernatant was removed and chromatin was extracted overnight at 4°C in 0.5X PBS (67.5 mM NaCl) with a protease inhibitor cocktail (Roche) on an end-over-end rotator ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitation was performed at 4 °C for 1 h with pre-conjugated anti-HA affinity matrix (Roche 11815016001) or Anti-GFP Agarose (RQ2 ...
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bioRxiv - Biophysics 2023Quote: ... 1a) in a 27°C water bath for 15 minutes and then quenched with 0.5 mM PMSF (Roche). Samples were analyzed on a Coomassie stained SDS-PAGE PAGE gel ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions were incubated at 37°C for 3-4 h before adding DNase I (0.1 U/µl) (Roche) and incubating for another 30 min at 37°C ...
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bioRxiv - Genomics 2023Quote: ... treated at 55°C and then pre-cleared with 1:2 KAPA Pure beads (Roche Cat. No: 07983298001). The quality and quantity of fragmented DNA were verified using agarose gel (1.5% ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell suspension was then chemically digested by incubation at 37°C for 30 min with collagenase (Roche #11088866001), dispase (Stemcell Technologies #07923 ...
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bioRxiv - Cell Biology 2023Quote: ... or MDCK-Ecad Res cells were pre-treated with Mitomycin C at a concentration of 10μg/ml (Roche) for a duration of 1 hour to inhibit cell proliferation ...
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bioRxiv - Immunology 2023Quote: ... followed by a subsequent incubation at 95L°C in pre-formulated Cell Conditioning 1 solution (Roche Diagnostics, Canada). Following this ...
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bioRxiv - Physiology 2024Quote: ... cut in small pieces and digested at 37°C with collagenase A at 1mg/kg (Sigma-Roche, #10103578001) in Krebs - 1% BSA buffer during roughly 20 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Both were fragmented at 94°C for 6 min and ligated with KAPA Unique Dual-Indexed adaptors (Roche). Library quality was checked on an AATI (now Agilent ...
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bioRxiv - Immunology 2021Quote: ... PBMC were seeded at 1 × 106 cells/mL in erythroid differentiation-promoting medium based on StemSpan™ Serum-Free Expansion Medium (SFEM) supplemented with human recombinant EPO (2 U/ml, Roche), human recombinant stem cell factor (25 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... The normalization of DNA amount was performed by quantifying albumin gene copies with qPCR using human genomic DNA (20 ng/µl) standard (Roche, #11691112001) as we previously described16.
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bioRxiv - Microbiology 2019Quote: ... while all other specific primers were designed to be compatible with the Human Universal Probe Library set (90 probes, octamer, Roche Diagnostics) (Supplementary Table 9 ...
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bioRxiv - Immunology 2019Quote: ... the wells were washed five times and incubated with 100 µl/well goat anti-human IgG conjugated with alkaline phosphatase (Roche Diagnostics) diluted 1 in 5,000 for 30 min at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then cultured with 2 μg/ml of plate-bound anti-CD3 and anti-CD28 monoclonal antibodies (αCD3αCD28 stimulation) (mAbs) (eBioscience) and 25 U/ml of recombinant human interleukin-2 (IL-2; Roche Applied Science) at a concentration of 1.5-2 × 106 cells/ml in RPMI supplemented with 10% heat-inactivated Human Serum (HS ...
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bioRxiv - Cell Biology 2021Quote: ... adapter-ligated libraries were prepared with the KAPA Hyper Prep Kit and sequencing libraries were constructed using SeqCap EZ Human Exome Library v3.0 (Roche, Basel, Switzerland). Cluster generation was performed with the HiSeq PE Cluster Kit v4 (Illumina ...
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bioRxiv - Cell Biology 2022Quote: ... then incubated for 30 min in Ringer solution before plating on fibronectin-coated glass coverslips (human plasma fibronectin at 10 µg/ml, Roche 10838039001).
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bioRxiv - Microbiology 2021Quote: ... and washed once in cell lysis buffer supplemented with 20U of human placental RNase inhibitor and cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche) prior to processing lysates ...
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bioRxiv - Microbiology 2021Quote: ... falciparum 3D7 or HEK 293F cells were suspended in 1× pellet volume of lysis buffer supplemented with 20U of human placental RNase inhibitor and cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche). Resuspended parasites were then transferred to a prechilled nitrogen cavitation chamber (Parr Instrument Company ...
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bioRxiv - Cancer Biology 2022Quote: ... Human samples were analyzed using a combination of gene-specific primers and Universal Probe Library (UPL) hydrolysis probes (Roche Life Science). Threshold cycle (Ct ...
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bioRxiv - Neuroscience 2020Quote: Total proteins from mouse cerebella or human cerebellar cortex were obtained by lysis in RIPA buffer containing protease inhibitors (Complete, Roche Diagnostics), followed by sonication and centrifugation using an established protocol in the laboratory31 ...
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bioRxiv - Neuroscience 2023Quote: ... prior to transfection with 2 μg pcDNA3.1 encoding N-terminally HA-tagged human 1N3R tau or 1N4R tau (14) using X-tremeGENE 9 (Roche Life Sciences), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... the T cells were activated in X-VIVO 15 containing 150 U/mL of human IL-2 (#Ro 23-6019, Roche, Switzerland), 10 ng/mL of recombinant IL-7 (#200-07 ...
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bioRxiv - Cancer Biology 2021Quote: ... paraffin-embedded human intestinal tissues was carried out on a Discovery Ultra automated slide stainer using a rabbit anti-human monoclonal antibody for CEA (Clone T84.66, Roche Glycart AG, Switzerland) at 2.23 µg/ml after antigen retrieval with Cell Conditioning 1 (CC1 ...
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bioRxiv - Microbiology 2020Quote: ... and 3-4 mice/group subcutaneously received 25ng/g of pegylated-human interferon α (Peg-hIFNα-2a) (Hoffmann La Roche, Basel, Switzerland). To activate IFN-1 signaling ...
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bioRxiv - Developmental Biology 2021Quote: ... Wing discs of L3 larvae were hybridized with probes overnight at 56 °C using standard procedures and visualized using anti-Dig-AP (1:1,000; Roche). Primers used for generating PCR templates are listed in Key Resources Table.
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bioRxiv - Genetics 2021Quote: ... Then they were incubated overnight at 4°C with primary antibodies diluted in a dilution buffer (0.5% blocking reagent (Roche), 2% fetal bovine serum ...
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bioRxiv - Microbiology 2021Quote: ... Epidermal and dermal sheets were prepared after incubation overnight at 4°C with 4 U/ml dispase II (Roche) in PBS by gently separating dermis and epidermis each as intact sheets (Rahn et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... Antisense DigoxigeninUTP-labeled RNA probes were synthesized at 37°C using RNA DIG labeling mix per manufacturer’s instructions (Roche) using RNA polymerase ...
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bioRxiv - Developmental Biology 2021Quote: ... Skin was cut into 1mm sized pieces and digested for 45 minutes at 37°C by 0.25 mg of Liberase DL (Roche) per mL of EHAA media and DNAse (Worthington) ...
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bioRxiv - Developmental Biology 2020Quote: ... they were hybridized overnight at 65°C for three nights and incubated overnight with 1/2,000 alkaline-phosphatase conjugated with anti-DIG Fab fragment (Roche) at 4°C for three nights ...
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bioRxiv - Developmental Biology 2020Quote: ... for 1 h and then incubated overnight at 4 °C in block solution with diluted DIG-antibodies (1:5,000) conjugated with alkaline phosphatase (AP) (Roche). To visualize WISH signal ...
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bioRxiv - Developmental Biology 2021Quote: ... treatment for 1 hour at 37°C and cDNAs were amplified with KAPA HiFi Hotstart Ready Mix (Roche, KK2602). PCR reactions were conducted as follow ...