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Citations for Roche :
501 - 550 of 2467 citations for 8 9 5 α CHOLESTEN 24 METHYLENE 3 β OL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... 10 mM β-mercaptoethanol) plus 1× cOmplete™protease inhibitor cocktail (Roche). The lysate was centrifuged at 22 ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mM β-glycerophosphate) and protease inhibitors (Protease Inhibitor Cocktail Tablets, Roche). Protein concentration was determined using a Bradford Protein Assay kit (Biorad) ...
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bioRxiv - Developmental Biology 2023Quote: ... and 1 mM β-glycerophosphate) with protease inhibitor cocktail (Roche, Indianapolis, IN) and 1 mM phenylmethylsulfonyl fluoride ...
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bioRxiv - Microbiology 2020Quote: Primary antibody dilutions were used as follows: rat α-HA antibody (Roche) 1:2,000 ...
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bioRxiv - Microbiology 2019Quote: ... Primary antibodies used were: 1 μg/mL rat α HA (Roche 3F10), 1/2000 rabbit α GFP (Abcam ab290) ...
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bioRxiv - Cell Biology 2019Quote: ... 1° antibodies used: α-GFP 1:1000 dilution (Mouse, Roche, Cat.No. 11814460001), α-Tubulin (TAT1 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated overnight with primary antibody α HA antibodies (Roche 3F10) (1:1000 dilution in 20% blocking solution I ...
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bioRxiv - Cell Biology 2022Quote: ... incubated overnight with primary antibodies (1:5000 α-TNY1, 1:10,000 Roche Rat-anti-HA ...
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bioRxiv - Microbiology 2022Quote: ... as loading control and incubated with a mouse α-GFP antibody (Roche). An HRP-coupled goat α-mouse antibody (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... Fusion proteins were detected with α-Myc monoclonal antibodies (Roche, Stockholm, Sweden). The following constructs were used ...
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bioRxiv - Developmental Biology 2023Quote: ... and 1 μl of Alkaline Phosphatase-linked α-Digoxigenin antibody (Roche, 11093274910) per sample ...
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bioRxiv - Biochemistry 2023Quote: ... blotted onto a PVDF membrane and probed with α-HA tag (Roche, Catalog# 11867431001 ...
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bioRxiv - Genomics 2020Quote: ... Pellets were resuspended in 10 mL of L2 buffer (200mM NaCl 1mM EDTA pH 8 0.5mM EGTA 10mM Tris, pH 8, dH2O, 1 protease inhibitor tablet (Roche Complete cat #1 697 498) per 50ml buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... left to attach for 24 hours and co-transfected using Lipofectamine 3000 (Roche) with (1 ...
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bioRxiv - Microbiology 2019Quote: ... 8 mM EDTA) with Complete™ protease inhibitor cocktail (Roche). The Triton X-100-insoluble material was pelleted twice by centrifugation (10 min at 16,000 g) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and phenol (phenol-Tris saturated, pH 8; Roche, Indianapolis, IN) and chloroform:isoamyl alcohol (American Bioanalytical ...
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bioRxiv - Biochemistry 2022Quote: ... pH=8) supplemented with EDTA-free protease inhibitor mixture (Roche) and 100 mM PMSF ...
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bioRxiv - Microbiology 2023Quote: ... and suspended in 8 M urea containing PhosSTOP (Roche Diagnostics) and Benzonase (Novagen) ...
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bioRxiv - Biophysics 2023Quote: Microchambers were incubated with 8 mg/µl anti-digoxigenin (Roche) at 25°C for 90 min and then the blocking buffer (PBS with 1% α-casein ...
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bioRxiv - Cell Biology 2021Quote: The plasmids FUG-T2A-GFP-Cre and control GFP were purchased from Addgene #66691 and lentiviral particles were produced in HEK-293T cells using X-tremeGene 9 DNA Transfection Reagent (Roche). Upon ultracentrifugation concentrated virus particles were stored at −80°C and used to infect pancreatic ductal organoid cultures as previously described (Huch et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmid and siRNA transfection into HeLa cells were carried out using X-tremeGENE 9 DNA transfection reagent (Roche) and Oligofectamine reagent (Invitrogen) ...
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bioRxiv - Developmental Biology 2019Quote: ... Transfection targeting the AAVS1 locus was performed with X-TremeGene 9 following the manufacturer’s instructions (Roche, Indianapolis, IN).
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bioRxiv - Developmental Biology 2019Quote: ... All three vectors were transfected into DLD-1 TIR1 cells using Xtreme Gene 9 DNA transfection reagent (Roche). Cells were sorted based on their YFP fluorescence and single clones were isolated ...
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bioRxiv - Developmental Biology 2021Quote: ... washing the pellet in PBS and resuspending the cells in ice-cold 10 ml buffer A (10 mM HEPES pH 7.9, 10 mM KCl, 1.5 mM MgCl2, 0.1% NP-40, c0mplete Mini Protease inhibitor EDTA free from Roche) and rotating for 10 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... A pre-amplification step of 9 PCR cycles was performed using the Kapa HiFI Hoststart kit (Kapa Biosystems). The PCR product was purified using AmpureXP beads (Beckman Coulter ...
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bioRxiv - Biochemistry 2023Quote: ... pCW57.1_yeastAOX 32 with the Delta-Vpr packaging plasmids and the VSV-G envelope plasmid (DNA concentration ratio, 1.2: 1: 0.3) into HEK293T cells using X-tremeGENE 9 Transfection Reagent (Roche). Lentivirus-containing media was harvested at 24 hr after fresh media change ...
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bioRxiv - Cell Biology 2023Quote: ... Fig-ure 3D,F,J) or 48 h (for Figure 4H) using X-tremeGENE 9 DNA reagent (Roche) following the manufacturer’s instruc-tions ...
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bioRxiv - Cell Biology 2023Quote: ... HEK-293T cells were transiently transfected using X-tremeGENE™ 9 DNA Transfection Reagent (Roche, Cat. # XTG9-R0) with 6 µL of transfection reagent to 2 µg plasmid for 48 hr ...
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bioRxiv - Cancer Biology 2020Quote: ... All the signals were visualized by adding 3-3′-Diaaminobenzidinetetrahydrochloride (DAB substrate) solution (Roche) to the slides and counterstained with haematoxylin ...
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bioRxiv - Cell Biology 2021Quote: ... 2 mM β-mercaptoethanol) and supplemented with complete EDTA-free cocktail tablets (Roche), 0.01 mg/ml DNase (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... and LacZ (β-D-galactosidase of E. coli (Hoffmann-La Roche, Basel, Switzerland) were used ...
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bioRxiv - Immunology 2020Quote: ... β-galactosidase (lacZ) activity was measured in total cell lysates using CPRG (Roche) as a substrate ...
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bioRxiv - Developmental Biology 2020Quote: ... 0.25 mM Na3VO4 and 50 mM β-glycerolphosphate) plus protease inhibitors (Roche, 04693132001) for 30 min on ice ...
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bioRxiv - Biophysics 2023Quote: ... solubilized with 1 % final concentration n-Dodecyl β-D-maltoside (Roche, Mannheim, Germany) and incubated for 5 min at 25 °C ...
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bioRxiv - Immunology 2023Quote: ... 10 mM β-mercaptoethanol) supplemented with cOmplete Mini EDTA-free protease inhibitors (Roche). After sonication ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were detected with primary antibodies mouse mAb α-GFP (Roche Diagnostics #11814460001), 1:1000 and rabbit α-PfHP1 12 ...
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bioRxiv - Microbiology 2019Quote: ... 1 μg α-AGO1 antibody (Agrisera)/g leaf tissue or 0.1 μg α-HA antibody (3F10, Roche or 12CA5)/g leaf tissue were used ...
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bioRxiv - Cell Biology 2022Quote: ... Antibodies used in Western blot analysis: monoclonal α-GFP (1:1000; mouse; Roche), α-GAPDH (1:3000 ...
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bioRxiv - Microbiology 2022Quote: ... and mouse anti-GFP in a dilution of 1:1000 (α-GFP, ROCHE). For all primary mouse antibodies an anti-mouse-HRP in a dilution of 1:2000 was used (GE Healthcare) ...
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bioRxiv - Microbiology 2023Quote: ... followed by washing and then probing with α-HA-peroxidase (1:1000, Roche).
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bioRxiv - Microbiology 2023Quote: ... GFP- or HA3x-tagged proteins were detected using α-GFP (1:1000; Roche) or α-HA3x (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... cDNA was amplified for 24 cycles using KAPA HiFi HotStart ReadyMix (Kapa Biosystems KK2601). After PicoGreen quantification ...
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bioRxiv - Microbiology 2020Quote: ... In brief: viral RNA was extracted with a MagNA Pure 24 system (Roche, Germany) and quantified with the LightMix Assay SARS-CoV-2 RdRP RTqPCR assay kit (TIB MOLBIOL ...
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bioRxiv - Neuroscience 2021Quote: ... Nematodes were resuspended in 8 M urea in 100 mM TRIS-HCl buffer (pH 8) containing cOmplete™ protease inhibitor cocktails (Roche Diagnostic Canada, Laval, QC, Canada) and aliquoted into reinforced 1.5 mL homogenizer tubes containing 500 μm glass bead homogenizer tubes with 25 mg of glass beads ...
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Evolutionary conserved aspects of animal nutrient uptake and transport in sea anemone vitellogenesisbioRxiv - Evolutionary Biology 2022Quote: ... and 3% Blocking Reagent (Roche) to the hybridization mix during overnight blocking and hybridization of the probe ...
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bioRxiv - Developmental Biology 2023Quote: ... and 3% Blocking Reagent (Roche) to the hybridization mix during overnight blocking and hybridization of the probe ...
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bioRxiv - Cancer Biology 2021Quote: ... 20mM Tris-HCl pH 8) supplemented with protease inhibitors (Roche, 04693116001), followed by a final washing step with TE 1X buffer ...
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bioRxiv - Biochemistry 2019Quote: ... pH 8) containing complete protease inhibitor (in 50ml) cocktail (Roche Molecular) and disintegrated with a French press (1100 psi) ...
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bioRxiv - Cell Biology 2020Quote: ... the specimen was digested in 8 mg/mL Collagenase D (Roche) and 4.8 U/mL Dispase II (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... pH 8] with EDTA-free Protease Inhibitor Cocktail (Roche, Basel, Switzerland) and were clarified by centrifugation ...