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Citations for Roche :
5201 - 5250 of 6323 citations for Rat Myristoylated Alanine Rich C Kinase Substrate MARCKS ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... 6-10ng of immunoprecipitated material was used for ChIP-seq library preparation using the KAPA Hyper prep kit (KAPA Biosystems). Libraries were sequenced on an Illumina HiSeq 2500 platform on SE50 mode.
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bioRxiv - Immunology 2019Quote: PFA fixed zebrafish larvae were used in combination with the In situ Cell Death Detection Kit (TMR and Fluorescein) (Roche). After O/N fixation larvae were washed and permeabilized for 45 min at 37°C using Proteinase K ...
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bioRxiv - Physiology 2019Quote: ... Total RNA was reverse-transcribed to complementary DNA (cDNA) using the Transcriptor First Strand cDNA Synthesis Kit (Roche, Indianapolis, IN). RT-PCR amplification was consisted of an initial denaturation step (95°C for 10 min) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Paraffin was dissolved with xylene and total RNA extracted using the High Pure FFPE RNA Micro Kit (Roche, Indianapolis, IN) or Qiagen miRNeasy FFPE kit ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: Antiviral azaBINOL B#24 was assessed for inhibition of HIV-1 reverse transcriptase using a commercially available colorimetric reverse transcriptase assay kit (Roche) according to the manufacturer’s instructions ...
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Dual functionality of the TasA amyloid protein in Bacillus physiology and fitness on the phylloplanebioRxiv - Microbiology 2019Quote: ... subtilis strains at the different time points were evaluated via terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) using the In-Situ Cell Death Detection Kit with fluorescein (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... The crosslinked chromatin was lysed in 130 uL of Buffer B (LowCell# ChIP kit, diagenode) supplemented with Complete protease inhibitor (Roche) and 20mM Na-butyrate (Sigma) ...
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bioRxiv - Plant Biology 2019Quote: ... Illumina Paired-End libraries were constructed for 280 accessions using the KAPA Hyper Prep Kit (Kapa Biosystems, Wilmington, Massachusetts, USA) following the manufacturer’s instructions (KR0961 – v5.16) ...
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bioRxiv - Molecular Biology 2020Quote: ... TUNEL staining was performed prior to secondary antibody incubation using the Biotin-based in situ cell death detection kit (Roche) and following the manufacturer instructions ...
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bioRxiv - Cell Biology 2019Quote: ... cells were washed with ice-cold DPBS and scraped in ice-cold kit lysis buffer (1 mL/107 cells) containing a cocktail of protease inhibitors (cOmplete, EDTA-free; Roche). Cells were then lysed in a Potter-Elvehjem homogenizer (~50 strokes) ...
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bioRxiv - Bioengineering 2019Quote: ... the entire miniprepped volume was used as template in a PCR reaction using the KAPA HiFi PCR Kit (Kapa Biosystems). A ten-fold dilution of this PCR product was used as template in a barcoding PCR adding Illumina adapter sequences and dual barcodes ...
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bioRxiv - Microbiology 2019Quote: ... And 1.0 μg total RNA was reverse-transcribed into cDNA using the transcriptor first-strand cDNA synthesis kit (Roche, China). qPCR was conducted using FastStart Universal SYBR Green Master (Rox ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: The cDNA encoding for the A33 construct was obtained by total RNA extraction from the HT-29 colorectal carcinoma cell line (High Pure RNA Isolation Kit, Roche), reverse transcription (Transcriptor reverse transcriptase ...
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bioRxiv - Genomics 2021Quote: ... were used to prepare single-end (SE) libraries with an insert size of 300 bp using a PCR-free workflow with the KAPA HyperPrep Kit (Roche) following manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... The library was prepared by the Genome Technologies core facility at JAX using the KAPA mRNA HyperPrep Kit (KAPA Biosystems), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... TUNEL staining was performed on testis sections according to the instructions provided with the cell death detection kit (11684795910, Roche). To reduce experimental variations ...
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bioRxiv - Immunology 2021Quote: Strand-specific ribosomal RNA depleted RNA-seq libraries were prepared from 1 µg of total RNA using the KAPA Stranded RNA-seq Kit with Ribo-Erase (Roche). Samples were prepared in at least triplicate for analysis ...
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bioRxiv - Microbiology 2021Quote: ... The 32P-radiolabeled probe was generated from a plasmid containing the entire HPV16 genome by radiolabeling using a Random Prime labeling kit (Roche).
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bioRxiv - Neuroscience 2020Quote: ... Pooled libraries were cleaned using a 0.6:1 ratio of Ampure beads and quantified on Qubit using HS DNA reagents and with the KAPA Library Quantification Kits for Illumina (Roche). Samples were sequenced on two separate rapid-runs on HiSeq2500 (Illumina) ...
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bioRxiv - Developmental Biology 2021Quote: ... were generated cloning each enhancer from the genomic zebrafish DNA DNA using KAPA Long Range HotStart PCR kit (Kapa Biosystems) and cloned into the E1b:GFP:AC-DS vector (102417 Addgene (Chong-Morrison et al. ...
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bioRxiv - Cell Biology 2021Quote: ... Further quantification of the adapter ligated fragments and confirmation of successful P5 and P7 adapter incorporations were assessed with the KAPA universal library quantification kit for Illumina (Roche) run on a ViiA7 real-time PCR system (Applied Biosystems) ...
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bioRxiv - Neuroscience 2021Quote: The RNA-Seq libraries were prepared from total RNA using KAPA Stranded mRNA-Seq Kit Illumina Platforms (Roche-Kapa Biosystems) with minor modifications ...
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bioRxiv - Neuroscience 2021Quote: The RNA-Seq libraries were prepared from total RNA using KAPA Stranded mRNA-Seq Kit Illumina Platforms (Roche-Kapa Biosystems) with minor modifications ...
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bioRxiv - Evolutionary Biology 2020Quote: RNA was extracted from 200 μl swab rectal samples or fecal pellets with the High Pure Viral RNA Kit (Roche) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Viral transport media was added to each pool at a 1:1 ratio for nucleic acid extraction performed on the Roche MagNA Pure 24 platform using the MagNA Pure 24 Total NA Isolation kit (Roche). Elution volume was set to 50 ul to concentrate viral RNA ...
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bioRxiv - Systems Biology 2020Quote: ... strand-specific RNA libraries were prepared using a KAPA Stranded RNA-Seq Library Preparation Kit (KAPA Biosystems, catalog no. KK8401) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... was extracted from inactivated samples (200 μL) using the MagNA Pure Compact instrument and MagNA Pure Compact Nucleic Acid Isolation Kit I (Roche Molecular Systems Inc ...
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bioRxiv - Microbiology 2021Quote: ... The tissues were processed for immunohistochemistry using the Discovery Ultra automated stainer (Ventana Medical Systems) with a ChromoMap DAB kit (Roche Tissue Diagnostics cat#760–159) (Roche Diagnostics Corp. ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then treated with the TUNEL reaction mixture according to the protocol (In situ Cell Death detection Kit, TMR red; Roche) and incubated for 1 h at 37°C in the dark ...
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bioRxiv - Neuroscience 2021Quote: RNA was reverse transcribed using the High Capacity RNA-to-cDNA kit and qPCR performed using a Roche LightCycler480 with SYBR Green I Master Mix (Roche) and the primers listed in Table S2 ...
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bioRxiv - Neuroscience 2020Quote: ... All qPCR reactions were performed in 10 μl volume in triplicates with 1× LightCycler 480 SYBR Green I Master kit (Roche) and primers listed in Supplementary Table 1 on LightCycler® 480 PCR instrument II (Roche) ...
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bioRxiv - Neuroscience 2021Quote: ... Detection and quantification of apoptosis in cell cultures was determined using a In Situ Cell Death Detection Kit (Roche, 11684795910) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Libraries were prepared by the Van Andel Genomics Core from 300 ng of total RNA using the KAPA RNA HyperPrep Kit with RiboseErase (v1.16; Kapa Biosystems). RNA was sheared to 300-400 bp ...
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bioRxiv - Physiology 2021Quote: ... After the concentration of DNA was evaluated by qPCR using the KAPA Library Quantification kit (KAPA Biosystems, Wilmington, MA, USA), the libraries were loaded onto an Illumina MiSeq sequencer and then sequenced using MiSeq Reagent kits v2 500 cycles (Illumina ...
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bioRxiv - Bioengineering 2021Quote: ... the probes were detected with anti-digoxigenin and anti-fluorescein antibodies conjugated with alkaline phosphatase (AP) and horseradish peroxidase (HRP) using AP detection kit (Roche) and tyramide signal amplification (TSA ...
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bioRxiv - Microbiology 2020Quote: ... DNA probes for 5S rRNA and SPA0079 were amplified with PCR using primers described in Table S2 and PCR DIG Probe Synthesis Kit (#11636090910, Roche). The reaction was performed with a total volume of 50 μl containing 0.2 μM of forward primer ...
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bioRxiv - Plant Biology 2021Quote: ... first strand cDNA synthesis was performed using mRNA with an oligo-dT primer using Transcriptor High Fidelity cDNA Synthesis Kit (Roche), according to the manufacturer′s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... The amplified fragments were then used to produce digoxigenin (DIG)-labeled sense and antisense RNA probes using a DIG RNA Labeling Kit (Roche). Whole-mount in situ hybridization was performed following (39) ...
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bioRxiv - Microbiology 2020Quote: ... gRNA encoding DNA sequences were amplified in a two-step nested PCR using KAPA HiFi HotStart ReadyMixPCR Kit (Kapa Biosystems) and sequenced on an Illumina NextSeq (High Output ...
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bioRxiv - Plant Biology 2021Quote: ... Gene transcription was quantified by qPCR using a LightCycler 480 SYBR Green I Master kit and LightCycler 480 (Roche, Switzerland). The PCR conditions were 95 °C for 10 min followed by 45 cycles of 95 °C for 10 s ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was reverse-transcribed to cDNA using a Transcriptor First Strand cDNA Synthesis kit (Roche Diagnostics BV, Almere, Netherlands). Viral genomic RNA was quantified by real-time quantitative RT-PCR specific for the RdRp gene of SARS-CoV-2 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Library-quality was assessed using Fragment Analyzer and libraries were quantified by qPCR using the Kapa Library Quantification Kit (Roche). Sequencing was performed on a NovaSeq S4 lane (Illumina ...
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bioRxiv - Biochemistry 2021Quote: ... A total of 1 μg of total RNA was reverse-transcribed with oligo dT primers using the Transcriptor High Fidelity cDNA Synthesis Kit (Roche) according to the manufacturer’s protocols ...
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bioRxiv - Genomics 2021Quote: ... RNA-seq libraries were constructed with removal of ribosomal RNA using the KAPA Stranded RNA-Seq kit with RiboErase (Roche). The RNA extraction and library preparation were performed by the same technician to minimize confounding effects ...
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bioRxiv - Microbiology 2020Quote: ... previously published assay (“one-step real-time RT-PCR E assay”) using the One-Step RT-qPCR kit (Roche Diagnostics). For each run ...
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bioRxiv - Microbiology 2019Quote: ... Sendai Virus (SeV) and Modified vaccinia Ankara (MVA)-gfp viral stocks were extracted using the High Pure Viral Nucleic Acid Kit (Roche). When indicated ...
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bioRxiv - Molecular Biology 2021Quote: Serum creatinine was determined using the creatininase/creatinase specific enzymatic method described by Bömer using a commercial kit (Alpha Laboratories Ltd. Eastleigh, UK) adapted for use on a Cobas Fara centrifugal analyser (Roche Diagnostics Ltd ...
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bioRxiv - Microbiology 2021Quote: Total nucleic acid was extracted from 253 resuspended vaginal swab samples using the MagNA Pure Compact Nucleic Acid Isolation Kit (Roche) [33] ...
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bioRxiv - Plant Biology 2021Quote: ... Purified DNA amplicons with flanking T7 and SP6 promoters were used for in vitro synthesis of digoxigenin (DIG)-UTP-labelled single-stranded RNA sense and anti-sense probes by using T7 and SP6 polymerases from a DIG RNA Labelling Kit (Roche). The non-complementary sense probe was used as negative control for each gene ...
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bioRxiv - Microbiology 2021Quote: ... Library preparation and sequencing data processing were performed according to Nilsen et al (2020) except the library was quantified with the KAPA library quantification kit (universal; Roche) in a CFX96 Touch Real-Time PCR Detection System (Bio-Rad ...