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Citations for Roche :
5201 - 5250 of 10000+ citations for Rat Insulin Like Growth Factor 1 Receptor IGF1R ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: GST-tagged CBX8 was purified by re-suspending thawed cell pellets in 30 ml of lysis buffer (1× PBS, 5 mM DTT, 1× EDTA free protease inhibitor cocktail (Roche Diagnostics, Indianapolis, IN)) per liter of culture ...
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bioRxiv - Biochemistry 2021Quote: ... The pellets of harvested bacteria were resuspended in 5 mL of lysis buffer (20mM NaP pH 7.5, 300mM NaCl, 15mM imidazole, 5% glycerol, 0.5mM TCEP, 1 mg/ml lysozyme, 5 U/ml DNase, 1 Roche protease inhibitor tablet/100mL) per 1 g of wet weight culture ...
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bioRxiv - Biochemistry 2021Quote: ... The pellets were resuspended in 5 mL of lysis buffer (20mM NaP pH 7.5, 300mM NaCl, 15mM imidazole, 5% glycerol, 0.5mM TCEP, 1 mg/ml lysozyme, 5 U/ml DNase, 1 Roche protease inhibitor tablet/100mL) per 1 g of wet weight culture ...
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bioRxiv - Microbiology 2020Quote: NUP153/NSP1-expressing cell pellets were resuspended in lysis buffer (1× PBS, 0.5 mM TCEP, 0.1 mM PMSF, 1× Roche cOmplete protease inhibitors, pH 7.4), and lysed in a homogenizer ...
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bioRxiv - Genetics 2019Quote: ... Sheered chromatin samples were pre-cleared by incubating with the 250ul aliquot of Dynabeads resuspended in 2ml of Dilution Buffer (20mM Tris pH=8, 2mM EDTA, 150mM NaCl, 1% Triton X-100, 1:50 Roche cOmplete protease inhibitor) for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Beads were spun down and washed twice in 1 mL of buffer (50 mM Hepes, pH 7.4, 150 mM NaCl, 1 mM DTT, cOmplete (La Roche Ltd., Basel, Switzerland, 05056489001)) and subjected to sample preparation for MS measurement ...
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bioRxiv - Microbiology 2024Quote: ... 10 mM NaCl, 1.5 mM MgCl2, 1 mM DTT, 100 μg/ml PMSF, 1 μg/ml pepstatin, and Roche Complete Protease Inhibitor Cocktail) with 100 U/ml of OmniCleave nuclease (Epicentre ...
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bioRxiv - Microbiology 2024Quote: ... 1.5 mM MgCl2, 0.1% Triton X-100, 1 mM DTT, 100 μg/ml PMSF, 1 μg/ml pepstatin, and Roche Complete Protease Inhibitor Cocktail), incubated for 20 minutes on ice ...
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bioRxiv - Microbiology 2024Quote: ... 1.5 mM MgCl2, 0.1% IGEPAL Nonidet P-40, 1 mM DTT, 100 μg/ml PMSF, 1 μg/ml pepstatin, and Roche Complete Protease Inhibitor Cocktail) and transferred to a Dounce homogenizer and homogenized with 20 slow strokes ...
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bioRxiv - Evolutionary Biology 2023Quote: ... in 10 ml of extraction buffer (pH 6.7) (250 mM sucrose, 120 mM KCl, 10 mM MOPS, 5 mM MgCl2, 1 mM DTT, 1 Roche protease inhibitor cocktail tablet) (Vought et al ...
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bioRxiv - Microbiology 2023Quote: ... and resuspended in 1 mL of buffer 1 (20 mM K-HEPES pH 7.9, 50 mM KCl, 10% glycerol, and Roche EDTA-free protease inhibitors). Subsequently ...
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bioRxiv - Biophysics 2023Quote: ... 1% Triton X-100, 10% glycerol, 10 mM NaH2PO4, 10 mM NaP2O7, 100 μM ZnCl2, 10 mM NaF, 1 Roche protease inhibitor tablet), lysed by sonication and the supernatant isolated by centrifugation ...
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bioRxiv - Neuroscience 2023Quote: ... 1 mM EDTA, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, protease inhibitor cocktail [Roche], and PhosStop [Roche]; pH 7.5) and sonicated for 30 s ...
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bioRxiv - Neuroscience 2023Quote: ... The slices were lysed in lysis buffer (50 mM Tris/HCl, 1 mM EDTA, 1% SDS, phosphatase inhibitor cocktail (Roche, Indianapolis, IN, USA), and protease inhibitor cocktail (Roche) ...
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bioRxiv - Cell Biology 2024Quote: ... followed by 1 hour incubation with the following primary antibodies diluted in blocking solution: mouse IgG1 mAb α-GFP (1:500, Roche Diagnostics #11814460001), mouse IgG2 mAb α-Centrin clone 20H5 (1:500 ...
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bioRxiv - Developmental Biology 2024Quote: ... and incubated overnight at 4°C with anti-Digoxigenin-AP-conjugated antibody (dilution range: 1:1000 to 1:5000, Roche, catalog no. 11093274910) diluted in the same buffer ...
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bioRxiv - Cell Biology 2024Quote: ... pH 8, 150 mm NaCl, 1 mm EDTA, 1% Triton X-100, and cOmplete™ EDTA-free Protease Inhibitor Mixture, Roche Applied Science) before clarification by centrifugation and enrichment for FLAG-tagged proteins using anti-FLAG® M2 Affinity Gel (A2220 ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 M NaCl, 30 mM imidazole, 1 mM DTT, 1 mM benzamidine, and one EDTA-free complete protease inhibitor cocktail tablet [Roche] per 50 ml). The lysate was clarified by centrifugation at 38,000 g in a fixed-angle rotor ...
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bioRxiv - Microbiology 2020Quote: ... and then labelled using the DIG High Prime DNA Labelling and Detection Starter Kit I (Roche).
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bioRxiv - Microbiology 2020Quote: ... Ziehl-Neelsen staining was performed using automated Benchmark Special Stains instrument and dedicated staining kit (Roche). Photos of immunohistochemical staining and Ziehl-Neelsen staining were taken using AxioCam HRc (Zeiss ...
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bioRxiv - Cancer Biology 2021Quote: ... total RNA was converted into cDNA using the High Capacity cDNA Reverse Transcription Kit (Roche #4368814) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... followed by exome target capturing with the SeqCap EZ exome probes V3 kit (Roche, Basel, Switzerland). Cluster generation was carried out on a cBOT system (Illumina ...
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bioRxiv - Cell Biology 2020Quote: Intracellular ATP levels were determined by an ATP Bioluminescence Assay Kit (Roche Applied Science, Mannheim, Germany), according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2020Quote: ... and 250ng was reverse transcribed into cDNA by Transcriptor First Stand cDNA Synthesis kit (Roche Diagnostics). Quantitative PCR reactions were performed with fast Start Universal Probe Master kit and UPL-probe system in a LightCycler 480 Instrument (Roche Diagnostics ...
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bioRxiv - Cell Biology 2020Quote: Total RNA from AEC bronchial brushings was isolated by High Pure RNA isolation kit (Roche Diagnostics) and 250ng was reverse transcribed into cDNA by Transcriptor First Stand cDNA Synthesis kit (Roche Diagnostics) ...
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bioRxiv - Immunology 2021Quote: ... and reverse transcribed to cDNA using a Transcriptor First Strand cDNA Synthesis Kit (Roche, Mannheim, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... Sequencing libraries were prepared using the LTP library preparation kit for Illumina platforms (Kapa Biosystems, Germany). The libraries were then processed for PCR (7 cycles ...
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bioRxiv - Developmental Biology 2021Quote: ... Digoxigenin- or FITC-labeled antisense probes were synthesized using an RNA Labeling Kit (SP6/T7; Roche). The staining was revealed with NBT/BCIP or INT/BCIP substrate (Roche) ...
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bioRxiv - Genomics 2021Quote: ... Final libraries were PCR-amplified during 5 cycles with Kapa HIFI PCR kit (Roche, Basel, Switzerland) before standard library quality control with standard sensitivity NGS Fragment kit (Agilent ...
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bioRxiv - Cancer Biology 2021Quote: ... The libraries were quantified using the Kapa Library Quantification Kit Illumina Platforms (Kapa Biosystems, Boston, MA). The denatured and diluted libraries were sequenced on a NextSeq 550 System (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... and were immediately subjected to reverse transcription with the Transcriptor First Strand cDNA Synthesis Kit (Roche). Each representative cDNAs of il-6 ...
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bioRxiv - Developmental Biology 2021Quote: ... All indexed libraries were quantitated by qPCR using the KAPA Library Quantification Kit (KAPA Biosystems #KK4854). Samples were sequenced on an Illumina NovaSeq using 100bp paired-end sequencing or an Illumina HiSeq 2500 instrument using 75 bp single-end reads ...
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bioRxiv - Developmental Biology 2021Quote: ... and PCR enriched (8-10 cycles) using the KAPA Hyper Library Preparation kit (KAPA Biosystems, #KK8504). eCLIP libraries were prepared as described above according to (Van Nostrand et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... Apoptotic cells were identified by using an in situ cell death detection kit (Roche, catalog #11684795910) according to the manufactureŕs instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... The digoxigenin-labelled antisense RNA probes were produced using a DIG RNA labeling kit (Roche, #11277073910) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... All 20 dpf libraries were prepared using the KAPA stranded mRNA-seq kit (KAPA Biosystems 2016) at the High Throughput Genomic Sequencing Facility at UNC Chapel Hill and sequenced on one lane of Illumina 150PE Hiseq4000 (McGirr and Martin 2018) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Libraries were constructed from the fragmented DNA using the KAPA HyperPrep Kit (Kapa Biosystems, South Africa) per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Large RNA libraries were constructed using KAPA Stranded RNA-Seq Kits with RiboErase (Cat. # 07962282001, Roche) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: Digoxigenin-labeled antisense Rp-BicC RNA probes were synthesized using the RNA-Dig Labeling kit (Roche). In situ hybridization was carried out in 4% PFA fixed ovaries stored in PBT at 4°C ...
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Planarian CREB-binding protein (CBP) gene family regulates stem cell maintenance and differentiationbioRxiv - Developmental Biology 2020Quote: ... Riboprobes for in situ hybridization were synthesized using the DIG RNA labelling kit (Sp6/T7, Roche) following the manufacturer’s instructions ...
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Neurotransmission and neuromodulation systems in the learning and memory network of Octopus vulgarisbioRxiv - Neuroscience 2021Quote: ... This was carried out using the DIG RNA Labeling Kit (SP6/T7) (Roche; Cat. No. 1175025) according to the manufacturer’s instructions (13µL template ...
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bioRxiv - Neuroscience 2021Quote: ... Labeled S and AS probes were generated using the DIG RNA Labeling Kit (SP6/T7) (Roche) per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The library was prepared by ligation of indexed adapter sequences using the KAPA HyperPrep Kit (Roche). Sequencing was performed using Illumina MiSeq (2 x 300-bp paired-end reads) ...
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bioRxiv - Molecular Biology 2020Quote: ... The concentration of the RNA-seq library was quantified by KAPA Library quantification kit (KAPA BIOSYSTEMS). mRNA sequencing was performed on a HiSeq 2000 platform (Illumina).
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bioRxiv - Molecular Biology 2020Quote: ... The concentration of the ChIP-seq library was quantified by KAPA Library quantification kit (KAPA BIOSYSTEMS). ChIP sequencing was performed on a HiSeq 2000 platform (Illumina).
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bioRxiv - Cell Biology 2022Quote: ... libraries were then quantified by qPCR using the KAPA Library Quantification Kit KK4835 (cat. 07960204001, Roche) prior to amplification with Illumina’s cBot ...
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bioRxiv - Genomics 2020Quote: ... concentrators and purified in large volume columns (High Pure Viral Nucleic Acid Large Volume Kit, Roche) using 10X (2.5 ml ...
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bioRxiv - Genomics 2020Quote: ... the quantification of the prepared libraries was performed using a KAPA Library Quantification Kit (KAPA Biosystems). They were sequenced on a HiSeq 1500 (Illumina ...
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bioRxiv - Immunology 2021Quote: ... also available at addgene (plasmid 15912) (http://www.addgene.org/15912/) using the Roche Rapid ligation kit (Roche). The correct insert orientation was confirmed by NheI and SmadI (NEB ...
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bioRxiv - Immunology 2021Quote: ... Reverse: 3’-GGTCCACCAAACGTAATGCG-5’) were performed using KAPA SYBR FAST ONE-STEP qRT-PCR kits (Roche) according to manufacturer’s instructions on a Lightcycler 480 Instrument-II (Roche).