Labshake search
Citations for Roche :
5101 - 5150 of 6565 citations for Estrone ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... RNA from mouse brainstem or primary cell cultures was isolated using the High Pure RNA Isolation Kit (Roche). HiPSC-derived cells were lysed in RLT Plus buffer (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... quantitative PCR (RT-qPCR) with the SensiFAST SYBR No-ROX One-Step Kit in a LightCycler 96 (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... 500 ng of RNA was prepared for sequencing using the KAPA RNA HyperPrep Kit with RiboErase (Kapa Biosystems). Libraries were sequenced on the NextSeq 500 (Illumina) ...
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bioRxiv - Microbiology 2023Quote: ... RNA was re-extracted from original material using the High Pure RNA Isolation Kit (Roche Diagnostics GmbH Mannheim). A multi segment RT-PCR amplification was performed using the Superscript III high-fidelity RT-PCR Kit (Invitrogen ...
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bioRxiv - Genetics 2023Quote: ... Libraries were quantified by qPCR using the Library Quantification Kit for Illumina sequencing platforms (KAPA Biosystems, cat#KK4824) using 7900HT Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Immunology 2023Quote: ... 100 ng total RNA was reverse transcribed into cDNA using Transcriptor First Strand cDNA Synthesis Kit (Roche, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... We quantified libraries by qPCR using the Library Quantification Kit for Illumina sequencing platforms (Kapa Biosystems, cat#KK4824) and the 7900HT Real Time PCR System (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2023Quote: ... The optimal numbers of PCR cycles were pre-determined using the KAPA Real-Time Library Amplification Kit (Roche) as described previously (Tanegashima et al ...
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bioRxiv - Physiology 2023Quote: ... the terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) in situ cell death detection kit (Roche, Basel, Switzerland) was used according to the manufacturer’s instructions (including incubation for 1 h at 37 °C) ...
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bioRxiv - Cancer Biology 2022Quote: ... or PPIA) was performed in duplicates with fast start universal SYBR Green master (ROX) PCR kit (Roche, 04913914001) using QuantStudio7 (Thermofisher) ...
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bioRxiv - Developmental Biology 2022Quote: ... Both probes were labeled with fluorescein or digoxigenin (DIG) using RNA labeling kit (Roche Applied Science, Mannheim, Germany). The heads of fish at 4 mpf were fixed in 4% paraformaldehyde (PFA ...
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bioRxiv - Genetics 2023Quote: ... cDNA was synthesized with Anchored-oligo (dT)18 primers and the Transcriptor First Strand cDNA Synthesis Kit (Roche). cDNA was diluted five-fold with water and 2-4μl of the diluted cDNA served as template for each qRT-PCR as described previously 4 ...
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bioRxiv - Developmental Biology 2023Quote: ... The library pool was quantified by qPCR using a Library Quantification Kit for Illumina sequencing platforms (Kapa Biosystems). Paired-end 75bp sequencing was carried out by clustering 1.5pM of the library pool on a NextSeq 500 sequencer (Illumina).
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bioRxiv - Molecular Biology 2023Quote: ... Libraries preparation were performed with a Kapa Hyper Prep Kits with KAPA Library Amplification Primer Mix (Roche, 07962347001), quantified by Qubit (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 ng of DNA were used to prepare libraries using the KAPA Hyper Prep Kit (Kapa Biosystems KK8504) with 8 cycles of PCR ...
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bioRxiv - Neuroscience 2023Quote: TUNEL staining was performed for paraffin sections using the In Situ Cell Death Detection Kit (Roche, Basel, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... LDH was measured using the Cytotoxicity Detection Kit according to the manufacturer’s instructions (ref: 11644793001, Roche, Basel, Switzerland) and absorbance (ABS ...
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bioRxiv - Plant Biology 2023Quote: ... transferred on nylon membrane and hybridized with DIG-labelled probes obtained by PCR DIG Probe Synthesis Kit (Roche) with specific primers (see table primers list) ...
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bioRxiv - Systems Biology 2023Quote: Libraries were quantified for sequencing using the KAPA Library Quantification Kit (optimized for Roche® LightCycler 480, 07960298001). Sequencing was performed on the Illumina® NovaSeq 6000 and NextSeq 2000 sequencing systems ...
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bioRxiv - Systems Biology 2023Quote: ... combining 80 μL of purified second strand with a final 1x KAPA HiFi Library Amp kit (Roche, #07958960001), 1μM of p5 Fwd primer and p7 indexing Rev primer in a volume of 200 μL ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were pooled in equal molar ratio and quantified with KAPA Library Quantification Kit for Illumina (Kapa Biosystems) and sequenced paired-end 50 on an Illumina NovaSeq 6000 instrument.
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bioRxiv - Microbiology 2023Quote: ZIK1 sense and antisense RNA probes labeled with digoxigenin were synthesized using the DIG Northern Starter Kit (Roche) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Reverse transcription was performed with 1 μg of purified RNA using Transcriptor First Strand cDNA synthesis KIT (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Sequencing library preparation and probe capture enrichment were performed according to the SeqCap EZ Developer Kit (Roche NimbleGen) or the SeqCap RNA Developer Enrichment Kit (Roche NimbleGen) ...
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bioRxiv - Microbiology 2023Quote: ... hybridization capture was performed using the SHS method according to the SeqCap EZ Reagent Kit Plus (Roche NimbleGen) with modifications to the number of hybridization captures and the number of post-capture ligation-mediated PCR (LM-PCR ...
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bioRxiv - Developmental Biology 2023Quote: ... Full length cDNA was then converted into an Illumina sequencing library using the Kapa Hyper Prep kit (Roche). In brief ...
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bioRxiv - Microbiology 2023Quote: ... An HBV RNA probe and GAPDH RNA probe were linearized and synthesized by digoxigenin RNA labeling kit (Roche). Hybridization and detection were performed using the DIG Northern Starter kit (Roche ...
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bioRxiv - Immunology 2023Quote: ... adapter ligation efficiency was confirmed through qPCR quantification using the Kapa Library Quant Kit by Roche (Catalog # 7960336001). All samples were sequenced on the Illumina NextSeq 550 sequencer platform with 150 cycles sequencing kits ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Library quantification was conducted using the Kapa Illumina GA with Revised Primers-SYBR Fast Universal kit (Kapa Biosystems). Fragment average size was determined using the LabChip GXII instrument (PerkinElmer) ...
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bioRxiv - Developmental Biology 2023Quote: ... In vitro transcription of Digoxigenin/Fluorescent-labeled probes was performed using an RNA Labeling Kit (Roche Diagnostics Corporation) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Isolated RNA was reverse-transcribed into cDNA using the Transcriptor First Strand cDNA Synthesis Kit (Roche Diagnostics, Switzerland). Expression of selected Nintedanib target genes was analyzed by SYBR Green quantitative PCR as described in 29 ...
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bioRxiv - Physiology 2024Quote: ... The total RNAs were converted into individually barcoded polyadenylated mRNAseq libraries with the Kapa HyperPrep mRNA kit (Roche). Libraries were barcoded with Unique Dual Indexes (UDI’s ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We prepared uniquely dual-indexed libraries for Illumina sequencing using the KAPA HyperPrep Kit (Roche, Indianapolis, Indiana, USA) using one-quarter reagent volumes ...
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bioRxiv - Microbiology 2024Quote: ... Draining lymph nodes were harvested and preserved in a lysis solution from High Pure DNA Isolation Kit (Roche).
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bioRxiv - Cell Biology 2024Quote: Libraries were constructed on 150 ng of input DNA with the KAPA HyperPlus kit (Roche Sequencing, CA, USA) according to manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2024Quote: AST and ALT levels were determined with a Kit (AST: Cat# 04467493190, Roche; ALT: Cat No 04467388190, Roche) according to International Federation of Clinical Chemistry and Laboratory Medicine (IFCC ...
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bioRxiv - Cell Biology 2024Quote: AST and ALT levels were determined with a Kit (AST: Cat# 04467493190, Roche; ALT: Cat No 04467388190, Roche) according to International Federation of Clinical Chemistry and Laboratory Medicine (IFCC ...
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bioRxiv - Developmental Biology 2024Quote: The RNA probes for detected genes were synthesized using a DIG RNA Labeling Kit (SP6/T7) (Roche, Germany). ISH was performed on 5 μm 4% paraformaldehyde-fixed paraffin-embedded sections from the testicular tissues ...
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bioRxiv - Neuroscience 2024Quote: ... quantified by qPCR using the Kapa Library Quantification Illumina/ABI Prism Kit protocol (KAPA Biosystems, Wilmington, MA, USA). Pooled libraries were paired-end sequenced on a High Throughput Run Mode flow cell with the V4 sequencing chemistry on an Illumina HiSeq 2500 platform (Illumina ...
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bioRxiv - Genomics 2024Quote: ... we constructed PE and SE libraries using the KAPA Hyper Library Preparation Kit (Kapa Biosystems, Woburn, MA, USA) and the Accel NGS 1S plus DNA kit (Swift BioSciences ...
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bioRxiv - Genetics 2024Quote: ... genomic DNA was extracted from mouse tail clippings and PCR amplified using the KAPA Mouse Genotyping Kit (Roche) following manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: The peptidyl-tRNAs in the PTS buffer were isolated by using a High Pure miRNA Isolation Kit (Roche), as described previously (54) ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA (2 µl) was added to the mixture with FastStart SYBR Green Master Mix kit (Roche, Basel, Switzerland) and specific primers SP_F 5’-GAC-CAG-TCG-AAC-GCA-CAT-TG-3’ and SP_R 5’-CGG-AGA-GGG-TTG-TTG-TGT-CT-3’ ...
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bioRxiv - Systems Biology 2024Quote: Paraffin-embedded tissue sections were processed with an in-situ cell-death detection kit (11684795910; Roche, Basel, Switzerland) for terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling ...
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bioRxiv - Molecular Biology 2020Quote: Cryosections (10μM) of ventricular tissue were fixed in 4% paraformaldehyde and stained with In-situ Cell Death Detection kit (Roche). Sections were imaged on a laser-scanning confocal microscope (LSM 510 ...
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bioRxiv - Developmental Biology 2021Quote: ... Digoxigenin-labelled antisense and sense RNA probes were synthesized by in vitro transcription using DIG-labelled UTP according to the manufacturer’s instructions (DIG RNA labelling kit, Roche). Primers used were as follow ...
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bioRxiv - Genomics 2020Quote: ... all multiplexed single-cell libraries (n = 30) were quantified using the KAPA Library Quantification Kit for Illumina Platforms (Roche) and pooled in an equimolar ratio ...
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bioRxiv - Developmental Biology 2021Quote: ... Pax3 digoxigenin (DIG) - labeled antisense riboprobes were transcribed from linearized gene-specific probes (PCR DIG probe Synthesis Kit, Roche). WISH experiment was performed as follows ...
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bioRxiv - Neuroscience 2021Quote: ... qPCR was performed on a Roche 480 LightCycler using the LightCycler 480 DNA SYBR Green I Master kit (Roche). In all cases ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA-seq libraries were generated using the KAPA HyperPrep Kit with RiboErase (HMR) and KAPA dual-indexed adapters (Roche Sequencing and Life Sciences ...