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Citations for Roche :
5051 - 5100 of 6383 citations for PD 1 Mouse HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... Beads were washed with Low Salt buffer (50 mM HEPES pH 7.5, 140 mM NaCl, 1% Triton X-100, 1x protease (Roche) and phosphatase inhibitors (Sigma-Aldrich)) ...
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bioRxiv - Genomics 2020Quote: ... Isolated nuclei were then lysed with lysis buffer (50mM Tris-HCl, pH 8.1, 10 mM EDTA, pH 8, 1% SDS, 1X protease inhibitor cOmplete EDTA-free cocktail, Roche). Nuclei were sonicated using a Bioruptor ultrasonic cell disruptor (Diagenode ...
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bioRxiv - Cancer Biology 2020Quote: ... pelleted at 500 × g for 10 min and lysed in TNTE buffer (1:10 wt:vol) supplemented with protease inhibitor cocktail (Roche) by homogenization using motorized Teflon-glass homogenizer (Thomas Scientific ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... 150mM NaCl, 10% glycerol, 5mM MgCl2, 0.1% Tween20, 15mM EGTA, 10μM MG132, and 1× cOmplete™ Protease Inhibitor Cocktail [Roche]) and incubated for 30 min at 8 rpm in the cold room ...
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bioRxiv - Molecular Biology 2020Quote: ... The sections were then rinsed with 2× SSC and 0.2× SSC and treated with alkaline phosphatase– conjugated anti-digoxigenin (anti-DIG, 1:500; Roche) for 2 h at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.1% Triton X-100) supplemented with Complete® protease inhibitor cocktail (Roche, 1 pastel per 10 mL lysis buffer) for 5 min ...
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bioRxiv - Neuroscience 2020Quote: ... pH 7.5, 150 mM NaCl, 5 mM EDTA, 2 mM ATP, 1 mM dithiothreitol and protease inhibitor cocktail tablets; Roche) using Bead Beater ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and lysed by sonication in prenylation buffer (50 mm HEPES, pH 7.2, 50 mm NaCl, 2 mm MgCl2, 100 μm GDP, 1× Roche complete EDTA-free protease inhibitor cocktail) ...
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bioRxiv - Biochemistry 2020Quote: ... bacterial pellets were resuspended using a high salt buffer (25 mM Tris pH7.5, 1 M NaCl, 10 mM imidazole, 5% glycerol) supplemented with protease inhibitor tablets (Roche), 0.5 mM PMSF ...
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Receptor-like role for PQLC2 amino acid transporter in the lysosomal sensing of cationic amino acidsbioRxiv - Cell Biology 2020Quote: ... 1% Triton X-100 plus protease and phosphatase inhibitor cocktail tablets (Complete mini, EDTA-free, Phos-STOP from Roche). Insoluble cell material was rejected by centrifugation ...
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bioRxiv - Synthetic Biology 2021Quote: ... 0.1% SDS) and resuspension of the nuclei in 0.9 mL Covaris shearing buffer (with 1× protease inhibitors complete mini (Roche)) ...
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bioRxiv - Physiology 2021Quote: ... we collected feces of 90 adults that had fed on Arabidopsis wild type leaves for one day in a total volume of 1 mL 20 mM MES buffer pH 6.5 containing protease inhibitors (cOmplete, EDTA-free, Roche). After homogenization with metal beads for 3 min at 25 Hz in a TissueLyzer II and centrifugation at 4 °C for 10 min at 16,000 × g ...
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bioRxiv - Microbiology 2021Quote: ... 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl PH8.0, 150 mM NaCl, complete protease inhibitor [Roche]), high salt (0.01% SDS ...
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bioRxiv - Molecular Biology 2021Quote: ... 1.2ml of Buffer NUN2 (300mM NaCl, 7.5mM MgCl2, 1% NP-40, 1M Urea supplemented with complete EDTA free protease inhibitor (Roche) and PhoSTOP (Roche ...
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bioRxiv - Neuroscience 2019Quote: ... and harvested in 200 μl lysis buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1% Triton-X100, 1X Complete protease inhibitor cocktail [Roche] ...
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bioRxiv - Plant Biology 2020Quote: ... Pellets were resuspended in buffer (50mM Tris-HCl pH 7.0, 100 mM NaCl, and 1 tablet anti-protease cocktail (Roche) for 25 mL) ...
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bioRxiv - Molecular Biology 2019Quote: ... The supernatants were removed and aggregated proteins were washed twice with 600 μl wash buffer (20 mM Na-phosphate pH 6.8, 1 mM PMSF, 1x protease inhibitor cocktail (Complete EDTA-free, Roche), 2% NP-40 ...
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bioRxiv - Molecular Biology 2019Quote: ... washed twice in ice-cold acetone then resuspended in 200 μl ice-cold extraction buffer (50 mM Tris-HCl pH 7.5, 1 mM EDTA, 1x protease inhibitors (Complete EDTA-free, Roche), 1% SDS) ...
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bioRxiv - Developmental Biology 2020Quote: ... 2003) except that 1 to 600 dilution of anti-DIG-HRP conjugated and anti-FLU-HRP conjugated antibodies (Roche 11207733910 and 11426346910 ...
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bioRxiv - Molecular Biology 2019Quote: For the generation of mass spectrometry samples the beads were washed 4 times with EBC-2 buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1 mM EDTA, and protease inhibitor cocktail (Roche)) and 2 times with 50 mM ammonium bicarbonate followed by overnight digestion using 2.5 µg trypsin at 37 °C under constant shaking ...
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bioRxiv - Cancer Biology 2020Quote: ... were resuspended in 50 μl PBS following the addition of 50 μl 1% SDS in 100 mM Hepes/NaOH pH8.5 supplemented with protease inhibitor cocktail EDTA-free (Roche). Samples were heated to 95°C for 5 min ...
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bioRxiv - Neuroscience 2020Quote: ... The brains were transferred to a 1.5 ml Eppendorf tube containing 500 microliters 1 mg/ml Liberase DH (Roche, prepared according to the manufacturer’s recommendation ...
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bioRxiv - Neuroscience 2019Quote: ... one series of frozen brain slices was homogenized in 1 ml PBS containing complete protease inhibitor (Roche Applied Science) and 1 mM AEBSF (Sigma ...
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bioRxiv - Neuroscience 2020Quote: Cultured cells were lysed in RIPA buffer containing protease inhibitor cocktail (1 tablet/15 mL) (Roche Diagnostics, Mannheim, Germany), sonicated and stored at −80°C until analysis ...
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bioRxiv - Molecular Biology 2019Quote: ... 200-500 mg of flower buds pooled from at least 4 individual plants were ground in liquid nitrogen or directly in 2 ml ice-cold lysis buffer (50 mM Tris HCL pH 7.5, 25 or 50 mM NaCl, 1% Triton, protease inhibitors (Complete - EDTA, Roche). After removal of cell debris by centrifugation (2 times 5 min ...
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bioRxiv - Cell Biology 2019Quote: Cells were harvested and lysed in ice-cold lysis buffer (20 mM Tris pH 8, 200 mM NaCl, 1 mM EDTA, 0.5% NP40) with protease inhibitor cocktail (Roche). Protein content of extracts was estimated using Pierce 660 nm Protein Assay Reagent (Thermo Fisher ...
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bioRxiv - Cancer Biology 2019Quote: ... 0,25 % NP-40 and supplemented with a protease inhibitor cocktail (1 tablets to 10 ml of lysis buffer, Roche) for 10 minutes at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... Q-RT pcr experiments were performed either by SYBR green or TaqMAN assay probes (Details provided in Supplementary table 1) according to manufacturer’s protocol using Lightcycler 480 II (Roche).
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bioRxiv - Molecular Biology 2019Quote: ... 150 nM each of PaaDlp-2_R1 and R2 in 1 × KAPA HiFi HotStart ReadyMix (KAPA Biosystems, Wilmington, MA, USA), and 3 µL sample eDNA ...
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bioRxiv - Genetics 2020Quote: ... Southern blot was performed according to the manufacturer’s protocol of DIG HIGH prime DNA labeling and detection starter kit 1 (Roche). 10 µg aliquot of genomic DNA was digested overnight with DraIII ...
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bioRxiv - Genetics 2021Quote: ... 0.1% SDS) and resuspension of the nuclei in 0.9 mL Covaris shearing buffer (with 1 × protease inhibitors complete mini (Roche)) ...
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bioRxiv - Cell Biology 2021Quote: ... 10 mM Tris HCl [pH 8.0], 10 mM MgCl2, 1 mM EGTA, and 1X protease-inhibitor cocktail [11697498001, Roche]), and incubated for 30 min at 4°C with gentle rocking ...
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bioRxiv - Cell Biology 2019Quote: ... Hi5 cells were harvested by centrifugation and resuspended in binding buffer (50 mM Tris, 300 mM NaCl, pH 8.0) with 1 mM PMSF and protease inhibitors (Complete, Roche). The cells were lysed by sonication and pelleted for 20 min at 21,000g using a 4°C Eppendorf 5424 centrifuge ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were dissociated in 1-2 ml of digestion mix (D-PBS, 250ug/ml Liberase TL (Roche; cat: 5401020001), 0.1 mg/ml DNaseI (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... The cell pellet was resuspended in 40 mL lysis buffer (50 mM NaH2PO4 pH 7.4, 300 mM NaCl, 1 mM PMSF and Complete protease inhibitors without EDTA (Roche)) and sonicated on ice ...
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bioRxiv - Microbiology 2021Quote: ... HEK293T cells were cotransfected with 4 μg of Env-deficient HIV-1 proviral plasmid (Q23ΔEnvGFP) and 2 μg of HIV-1 Env clone of interest using Fugene 6 transfection reagent (Roche) following manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... 50 mM NaCl; 20 mM Tris-HCl [pH 7.5]; 1 mM EDTA, 10% glycerol; complete protease inhibitor cocktail [Roche]). Samples were mixed (v/v ...
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bioRxiv - Cell Biology 2020Quote: ... and cells were lysed with TNE-lysis buffer (50 mM Tris, pH 7.4, 150 mM NaCl, 2 mM EDTA, 1% NP40, and complete protease inhibitors, Roche) and prepared for SDS-polyacrylamide gel electrophoresis (SDS-PAGE).
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bioRxiv - Developmental Biology 2021Quote: ... 2011) or Tyramide Signal Amplification (TSA) after incubation with anti-digoxigenin-AP or anti-FITC-POD (Roche, 1:300) antibodies ...
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bioRxiv - Developmental Biology 2021Quote: ... and resuspended in SDS lysis buffer (50 mM Tris-HCl [pH 8.1], 1% SDS, 10 mM EDTA) with complete protease inhibitor cocktail (Roche). Cell extracts were sonicated with a Bioruptor TOS-UCW-310-EX (output ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated overnight at 4°C in B1 buffer with alkaline phosphatase-conjugated anti-DIG (1/2000; Roche 11633716001). After three washes in B1 buffer and one wash in B3 buffer (100 mM Tris–HCl pH 9 ...
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bioRxiv - Genomics 2021Quote: ... Samples were then incubated overnight at 4°C with a rabbit anti-DIG HRP-conjugate antibody (1:500, Roche). Then ...
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bioRxiv - Neuroscience 2022Quote: ... Brain tissues were homogenized using mortar/pestle with liquid nitrogen and then lysed in ice-cold RIPA buffer (50mM Tris-HCL pH=7.4, 150mM NaCl, 1% Triton X-100, 0.1% SDS) (Beyotime, P0013B) freshly supplemented with protease inhibitor (Roche, 11836145001) and phosphatase inhibitor (Roche ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were harvested by centrifugation and resuspended in lysis buffer (50 mM HEPES pH7.5, 2M NaCl, 5 mM MgCl2, 1 tablet of Complete Inhibitor cocktail EDTA Free (Roche) per 50◻ml ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 30 animals per group were dissected in CMFB (CMF containing 0.5% BSA) and dissociated using 1:50 Liberase (2.5mg/mL, Roche 5401135001) in CMFB at 30°C with agitation at 300 rpm for 30 minutes on an Eppendorf ThermoMixer ...
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bioRxiv - Developmental Biology 2020Quote: Transfected HEK cells were lysed in cold immunoprecipitation buffer (1% NP-40, 50 mM Tris-HCl, pH 8.0, 150 mM NaCl) with protease inhibitors (Roche), and centrifuged for 10 minutes and 3,000 rpm at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... embryos were incubated overnight at 4°C with 1:2000 alkaline phosphatase-conjugated anti-DIG antibodies (Sigma/Roche, 11093274910). After washes and prior to development ...
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bioRxiv - Developmental Biology 2021Quote: ... 20% heat-inactivated lamb serum in Tris-buffered saline with 0.1% Tween-20 (TBST) and incubated with alkaline phosphate-conjugated anti-DIG antibody (1:2000, Roche) in blocking buffer overnight at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... for 2hr at room temperature and then incubated in blocking solution containing anti-Digoxigenin-AP antibody (1:2000; Roche) overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... 50 ng of nick translated probe per coverslip was combined with 12 μg of human Cot-1 DNA (Roche), 10 μg salmon sperm ssDNA (Sigma-Aldrich ...