Labshake search
Citations for Roche :
5001 - 5050 of 5471 citations for Rat Malonyl Coenzyme A Decarboxylase MLYCD ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Libraries were constructed by amplifying 500 ng of total RNA using 9 PCR cycles with the KAPA mRNA HyperPrep Kit (KAPA Biosystems). cDNA libraries were validated using the Bioanalyzer 2100 on a High Sensitivity DNA assay and quantified using the KAPA Library Quantification Kit for Illumina Platforms on a Roche Lightcycler 480 ...
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bioRxiv - Cancer Biology 2023Quote: The cellular metabolic viability was measured by NADH-dependent mitochondrial dehydrogenase activity using Cell Proliferation Kit II (XTT) (Roche, Basel, Switzerland) according to the manufacturer’s protocol by measuring the absorbance at 492nm in BioTek Synergy HTX multimode reader ...
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bioRxiv - Immunology 2023Quote: ... Apoptosis also was detected using a terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP)-biotin nick end-labeling (TUNEL) assay with Cell Death Detection Kit (Roche, Germany). Nuclei were stained with 4-6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Immunology 2023Quote: ... One step real time RT-PCR was performed using 100 ng of total RNA and KAPA SYBR FAST One-Step qRT-PCR Kits (Kapa Biosystems) according to the directions provided by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... to estimate the quantity and validate the size distribution and were then quantified by qPCR using the KAPA Library Quantification Kit KK4835 (Roche; #07960204001) before the amplification with Illumina’s cBot ...
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bioRxiv - Cancer Biology 2023Quote: ... in addition to qPCR on the Applied Biosystems QuantStudio 5 Real-Time PCR System using the Roche KAPA Library Quantification Kit (Roche, KK4824) to determine the concentration of adapter-ligated libraries ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were prepared from 2.5 ng of each sample using either the KAPA HyperPlus Kit (replicate 1: Roche/KAPA Biosystems, KK8512) or NEBNext Ultra II DNA Library Prep Kit for Illumina (replicate 2 ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were prepared from 2.5 ng of each sample using either the KAPA HyperPlus Kit (replicate 1: Roche/KAPA Biosystems, KK8512) or NEBNext Ultra II DNA Library Prep Kit for Illumina (replicate 2 ...
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bioRxiv - Neuroscience 2023Quote: ... The libraries were quantified by fluorometry on a Qubit instrument (LifeTechnologies) and by qPCR with a Kapa Library Quant kit (Kapa Biosystems-Roche) prior to sequencing ...
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bioRxiv - Microbiology 2023Quote: We used genomic DNA extracted by physical disruption for shotgun library construction with a KAPA Hyper Prep kit for Illumina (KAPA Biosystems)69 ...
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bioRxiv - Immunology 2023Quote: ... The concentration of each library was accurately determined through qPCR utilizing the KAPA library Quantification Kit according to the manufacturer’s protocol (KAPA Biosystems/Roche) to produce cluster counts appropriate for the Illumina NovaSeq6000 instrument ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... RNA libraries for sequencing were prepared using a KAPA Stranded mRNA Sample Preparation Kit (KK8401-07962169001, Kapa Biosystems, Wilmington, MA, USA). The libraries were sequenced after onboard cluster generation using HiSeq Rapid SBS Kit v2 (200 cycles ...
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bioRxiv - Evolutionary Biology 2023Quote: ... A total of 600 µL of serum or CSF was used for qPCR according to the standard protocol of the COMBAS TaqMan 96 HBV Test V2.0 Kit (Roche, IN, USA), and qPCR was performed with the Roche COBAS Ampliprep PCR system (Roche ...
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bioRxiv - Genomics 2023Quote: ... mRNA was enriched by poly-A capture and mRNA-seq libraries were generated using KAPA Stranded mRNA-seq Kit (KAPA Biosystems). Libraries were sequenced using the Illumina HiSeq 1500 platform.
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bioRxiv - Microbiology 2023Quote: ... Southern blotting was conducted to confirm the correct deletion using the digoxigenin (DIG) high prime DNA labeling and detection starter Kit I (11745832910 Roche Germany). The Myb gene complementation vectors were constructed by cloning the entire length of the target gene with the native promoter region (about 1.5-kb ...
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bioRxiv - Cell Biology 2023Quote: Library preparation was performed with 500 ng of total RNA where the ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems), fragmented in reverse transcriptase buffer and heated to 94°C for 8 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative real-time PCR (hereafter called qPCR) was performed by using the KAPA SYBR FAST qPCR Kit Master Mix (2×) Universal (KAPA Biosystems) with StepOne plus (Applied Bio systems) ...
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bioRxiv - Cancer Biology 2022Quote: ... The pooled samples with different indices for multiplex sequencing were measured using the library quantification kit (Kapa Biosystems, Wilmington, MA, USA) to obtain molar concentrations ...
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bioRxiv - Systems Biology 2022Quote: ... CRISPR gRNA encoding DNA sequences were amplified in a two-step nested PCR using KAPA HiFi HotStart ReadyMixPCR Kit (Kapa Biosystems). In the first PCR step ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The libraries were diluted 1:10 000 and 1:20 000 and quantified in triplicate using the KAPA Library Quantification Kit (Kapa Biosystems). Equimolar samples were pooled ...
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bioRxiv - Neuroscience 2023Quote: ... 2 µg of RNA was reverse-transcribed using the Transcriptor First Strand cDNA Synthesis Kit with random hexamer primers (Roche, 04379012001), and a 370-bp fragment (nucleotides 1304- 1673 of NM_010610.3 ...
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bioRxiv - Genomics 2023Quote: ... and Illumina PCR-free libraries were prepared from 700 ng DNA using the KAPA Hyper prep kit and unique dual-indexed adapters (5 µL of a 15 µM stock) according to the supplier’s protocol (Roche, Basel, Switzerland). The library concentration and size distribution were assessed on a Bioanalyzer (Agilent Technologies ...
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bioRxiv - Neuroscience 2023Quote: Plasmids were obtained from Transomic Technologies and the antisense mRNAs were synthesized using the Digoxigenin RNA Labeling Kit (SP6/T7; Roche, 11175025910). DIG RNA in situ hybridization (ISH ...
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bioRxiv - Neuroscience 2023Quote: ... Libraries were then quantified at the Genome Quebec Innovation Centre (Montreal, Quebec) using a KAPA Library Quantification kit (Kapa Biosystems, USA), and average fragment size was determined using a LabChip GX (PerkinElmer ...
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bioRxiv - Cell Biology 2023Quote: ... libraries were prepared in an automated fashion using an Agilent Bravo robot with a KAPA Standard mRNA-Seq Kit (KAPA BIOSYSTEMS). In house adaptors were ligated to 100-300 bp fragments of dsDNA ...
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bioRxiv - Immunology 2023Quote: ... DNA was incubated for 10 minutes at 65°C before performing RT qPCR using the KAPA SYBR FAST qPCR Kit from KAPA Biosystems. Primers used for real-time qPCR can be found in Supplementary Table 1.
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bioRxiv - Developmental Biology 2023Quote: TUNEL staining was performed according to the manufacturer’s protocol with minor modifications (In Situ Cell Death Detection Kit, TMR red; catalogue number 12156792910; Roche, Mannheim, Germany). Briefly ...
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bioRxiv - Zoology 2023Quote: The PCR products amplified from both IHHNV-309 and IHHNV-389 methods were used as templates to prepare digoxigenin (DIG)-labeled IHHNV probes using a PCR DIG-labeling kit (Roche, Germany). After preparation ...
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bioRxiv - Cell Biology 2023Quote: DNA was extracted from a cell suspension containing approximately 5x 105 cell/ ml using MagNA Pure 96 DNA and Viral NA Small Volume Kit in Magna pure 96 as instructed by the manufacture (Roche, Germany). DNA concentration was measured by NanoDrop 2000 instrument (Thermo Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... Digoxigenin-labeled sense and antisense RNA probes based on the sequence of TaABI3-A1 were synthesized using a DIG northern Starter Kit (Roche, 11277073910), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA sequencing (RNA-seq) libraries were prepared from 4 µg of total RNA using the KAPA stranded mRNA-seq kit (Roche, KK8421) according to manufacturer’s specifications ...
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Dysregulated expanded endocannabinoid system as therapeutic targets of amyotrophic lateral sclerosisbioRxiv - Neuroscience 2024Quote: ... The libraries were quantified using KAPA Library Quantification kits for Illumina Sequencing platforms according to the qPCR Quantification Protocol Guide (KAPA BIOSYSTEMS) and qualified using the TapeStation D1000 ScreenTape (Agilent Technologies) ...
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bioRxiv - Systems Biology 2024Quote: ... Shotgun metagenomic sequencing libraries were prepared using a miniaturized version of the Kapa HyperPlus Illumina-compatible library prep kit (Kapa Biosystems). DNA extracts were normalized to 5 ng total input per sample using an Echo 550 acoustic liquid handling robot (Labcyte Inc) ...
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bioRxiv - Molecular Biology 2024Quote: ... The V4–V5 regions of 16S rRNA gene were amplified using specific primers and libraries were constructed using the Hyper Library Preparation Kit from Kapa Biosystems (Roche Diagnostics ...
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bioRxiv - Microbiology 2023Quote: ... Indexed libraries that meet appropriate cut-offs for both are quantified by qRT-PCR using a commercially available kit (KAPA Biosystems) and insert size distribution determined with the LabChip GX or Agilent Bioanalyzer ...
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bioRxiv - Microbiology 2023Quote: ... Library prep included fragment end treatment, A-tailing, and ligation of Illumina-compatible adapters (IDT, Inc.) using the Kapa-HyperPrep kit (Kapa Biosystems). DNA was then sequenced 2 x 150 bp on the Illumina NovaSeq 6000 platform using S4 flow cells ...
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bioRxiv - Microbiology 2023Quote: RNA from specimens from Cyprus underwent automated total nucleic acid extraction using the MagNA Pure 96 DNA AND Viral NA Small Volume kit (Roche Diagnostics). RT-PCR for FCoV was performed at Laboklin Bad Kissingen ...
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bioRxiv - Microbiology 2023Quote: ... genomic DNA was fragmented using a Covaris S220 ultrasonicator and libraries were generated using KAPA LTP Library Preparation Kit (Roche, KK8230). To verify that the insertion was present in the genome at the correct location in both transfected lines ...
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bioRxiv - Neuroscience 2023Quote: A total of 1.0 µg of extracted DNA was used for PCR-free library construction using the KAPA HyperPrep PCR-Free Library Prep kit (Roche, KK8505). Mechanical shearing using the Covaris microtube system (Covaris ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 µg of RNA from each sample was processed using the KAPA Stranded mRNA-Seq Kit with mRNA Capture Beads (Kapa Biosystems). Sequencing was performed using NextSeq 500 (Illumina) ...
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bioRxiv - Cell Biology 2023Quote: Libraries were pooled in equimolar amounts and the final library pool was quantified using the KAPA Library Quantification Kit for Illumina Platforms (Roche; KK4824). Libraries were sequenced on the Illumina NextSeq 500 over two NextSeq 500 High Output v2.5 150 cycle kits (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... The RNA was then extracted with the Viral NA Small volume kit (06 543 588 001, Roche Diagnostics GmbH (Mannheim, Germany)) on the MagNA Pure 96 system (Roche Diagnostics GmbH (Manheim ...
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bioRxiv - Molecular Biology 2023Quote: Automated dual indexed FFPE libraries were constructed with 50-250 ng of genomic DNA utilizing the KAPA HTP Library Kit (KAPA Biosystems) on the SciClone NGS instrument (Perkin Elmer ...
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bioRxiv - Neuroscience 2023Quote: ... All purified nucRNA (9-20 ng/sample) was used to construct cDNA sequencing libraries using the KAPA RNA HyperPrep Kit with RiboErase (KK8560, KAPA Biosystems), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The concentration of each library was accurately determined through qPCR utilizing the KAPA library Quantification Kit according to the manufacturer’s protocol (KAPA Biosystems/Roche) to produce cluster counts appropriate for the Illumina NovaSeq6000 instrument ...
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bioRxiv - Cell Biology 2023Quote: ... 5 ng of DNA from each sample was analyzed using the Kapa Cyber Fast Q-PCR Kit (Kapa Biosystems, Wilmington, MA). The following rDNA primers (designed using Primer3Plus software ...
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bioRxiv - Cancer Biology 2023Quote: ... and quantified using the qPCR-based quantification in order to ensure only NGS-compatible amplicon was quantified using the Library Quant ROX Low Kit (Kapa Biosystems) on a QuantStudio™ 6 Realtime PCR System (ThermoFisher) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and amplified 6 times (Sets 1 and 2) or 8 times (Set 3) with a KAPA Library Amplification Kit (KAPA Biosystems). The amplification products were cleaned using Agencourt Ampure XP reagent or KAPA Pure Beads ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries were pooled in equimolar amounts and final library quantification performed using the KAPA Library Quantification Kit for Illumina (Roche; KK4824).
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bioRxiv - Genetics 2024Quote: ... along with a single -RT sample for each corresponding biological replicate using 2 μl cDNA and a no template control (NTC) with the LightCycler® 480 SYBR Green I Master kit (Roche). Ct values were measured with the Bio-Rad C1000 CFX96 Real-Time System and Bio-Rad CFX Software (qPCR primers ...