Labshake search
Citations for Roche :
4951 - 5000 of 5959 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
Convergence, plasticity, and tissue residence of regulatory T cell response via TCR repertoire prismbioRxiv - Immunology 2023Quote: ... and each lobe was cut into small pieces and digested with Collagenase A (Roche, 1mg/mL), DNAse (30 ug/mL ...
-
bioRxiv - Immunology 2023Quote: ... lymph nodes were manually minced into pieces and digested with collagenase A (1 mg/mL; Roche) and DNAse (10 µg/mL ...
-
bioRxiv - Biochemistry 2024Quote: ... 35 mM imidazole) + protease inhibitors (1 tablet / 50 ml Roche Complete EDTA-free, 1 mM AEBSF), lysozyme ...
-
bioRxiv - Cancer Biology 2023Quote: ... Collected media was incubated for 2 hours at 55°C with 20mg/ml proteinase K (Roche). Then ...
-
bioRxiv - Cell Biology 2023Quote: ... mouse ventricular cardiomyocytes were freshly isolated by Langendorff perfusion with Liberase TM (0.225 mg/mL, Roche) in Krebs-Henseleit buffer (135 mM NaCl ...
-
bioRxiv - Cell Biology 2023Quote: ... It was then minced and digested in HBSS containing 10 mg ml-1 Collagenase D (Roche), 3 U ml-1 Dispase II (Roche) ...
-
bioRxiv - Cell Biology 2023Quote: ... Infected cells were cultivated in the presence of IL-2 (10 IU/mL, Roche, Basel, Switzerland). Three days after coinfection cells were sorted based on expression of mouse CD48 and mCD24 (BD biosciences ...
-
bioRxiv - Molecular Biology 2023Quote: ... and filtered prior to loading onto a prepacked 5 mL cOmpleteTM His-Tag purification column (Roche) equilibrated in HBS ...
-
bioRxiv - Immunology 2023Quote: ... kidneys were then incubated for 15 minutes in DMEM containing 1 mg/ml Collagenase D (Roche), 100U/ml DNAse I (Sigma) ...
-
bioRxiv - Microbiology 2023Quote: ... PBS was carefully removed and replaced with 10ml DMEM containing 0.2 mg/ml DNase (Roche 10104159001) and 1 mg/ml Collagenase (Sigma-Aldrich C5138) ...
-
bioRxiv - Cell Biology 2023Quote: ... via in situ pancreas perfusion with 0.8 mg/ml collagenase P (Roche; 11-213-873-001) followed by pancreas digestion and islet purification using Histopaque 1077 and 1119 (Sigma) ...
-
bioRxiv - Cancer Biology 2023Quote: ... the lung pieces were digested in PBS supplemented with 75 µg/ml TH Liberase (Roche 05401127001), 75 µg/ml TM Liberase (Roche #05401151001) ...
-
Extrusion fountains are hallmarks of chromosome organization emerging upon zygotic genome activationbioRxiv - Molecular Biology 2023Quote: 400-600 embryos were homogenized in 2 ml 0.5 % Danieau’s with protease inhibitor cocktail (PIC, Roche) and 1 % (v/v ...
-
bioRxiv - Immunology 2023Quote: ... They were incubated in digestion buffer (1X PBS Ca+Mg+ containing 0.1mg/ml DNase I, Roche 05401127001 ...
-
bioRxiv - Immunology 2023Quote: ... tumors were cut into small pieces and enzymatically digested using 1mg/ml Collagenase D (#11088882001, Roche), and 0.1mg/ml DNase I (#101104159001 ...
-
bioRxiv - Cell Biology 2023Quote: Mouse ventricular myocytes were freshly isolated by Langendorff perfusion with Liberase TM (0.225 mg/mL, Roche) in Krebs-Henseleit buffer (135mM NaCl ...
-
Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... Subsequently 50% glycerol buffer (50ml H2O, 50 ml Ultrapure Glycerol, 1 Tablet protease inhibitor cocktail (Roche)) was added (final concentration 12% ...
-
bioRxiv - Immunology 2023Quote: ... fresh sinonasal tissue samples underwent enzymatic digestion with a mixture of 25mg/mL collagenase D (Roche Diagnostics GmbH ...
-
bioRxiv - Biochemistry 2023Quote: ... The clarified lysate was incubated with 2 ml of pre-equilibrated Protein C affinity resin (Roche) for 2h at 4°C ...
-
bioRxiv - Biophysics 2023Quote: ... and the brains were digested with 2.5 mg/mL Liberase™ (Thermolysin Medium) Research Grade (Roche) in 1X dissection saline and placed on a tube revolver rotator (ThermoFisher Scientific ...
-
bioRxiv - Cancer Biology 2023Quote: ... BM was flushed and dissociated in PBS containing DNase I (10 U/ml, Pulmozyme®, Roche) and Liberase DH (0.025 mg/ml ...
-
bioRxiv - Microbiology 2023Quote: ... The tissue pieces were incubated in a digestion cocktail of 300μg/ml Liberase TM (Roche, UK) and 50U/ml of DNaseI (Sigma-Aldrich ...
-
bioRxiv - Physiology 2023Quote: ... Tissue was enzymatically digested using 0.3 mg/ml collagenase (Type II, Worthington or Type A, Roche) and 1.25 mg/ml pancreatin from porcine pancreas (Sigma ...
-
bioRxiv - Microbiology 2023Quote: ... 240 μL of 80 mg mL−1 DNase I from bovine pancreas (Roche Diagnostics, Mannheim, Germany) was added along with MgCl2 to a final concentration of 30 mM ...
-
bioRxiv - Immunology 2023Quote: ... Total uteri and draining lymph nodes were digested with DNase I 200 units/mL (Roche 10104159001) and Liberase DL 0.3 Wunits/mL (Roche 05466202001 ...
-
bioRxiv - Immunology 2023Quote: Mouse lungs were isolated and placed in RPMI1640 containing Liberase Blendzyme 3 (70 μg/ml; Roche) and DNase I (50 μg/ml ...
-
bioRxiv - Neuroscience 2023Quote: ... DRG were incubated for 1 hour at 37 °C with 1 mg/ml dispase II (Roche) and 2 mg/ml collagenase A (Roche ...
-
bioRxiv - Microbiology 2023Quote: ... 720 μL concentrated solution was treated with 1000U/mL DNase I (37°C, 2h) (Roche, China) before viral DNA extraction ...
-
bioRxiv - Immunology 2024Quote: ... Left lung lobes were dissociated by enzymatic digestion with 1 mg/mL collagenase D (Roche, 11088882001), and 1 mg/mL collagenase IV (Worthington Biochemical ...
-
bioRxiv - Bioengineering 2024Quote: ... resuspended in PBS supplemented with 50 μg/mL DNAse I and EDTA-free protease inhibitors (Roche), and lysed with a French press ...
-
bioRxiv - Immunology 2024Quote: ... samples were incubated for 10 min at RT with DNAse I (50 µg/ml; Roche, 10104159001) and Dasatinib (50 nM ...
-
bioRxiv - Cell Biology 2024Quote: ... The slides were treated with a hybridizing solution (70% formamide, 1 mg/mL blocking reagent (Roche), and 10 mM Tris-HCl pH 7.2 ...
-
bioRxiv - Physiology 2024Quote: ... pH 7.4] and perfused with the same medium containing 120 μg/ml of Liberase (Roche, 05401127001). Thin kidney slices were cut along the corticomedullary axis and incubated at 30°C for 25 minutes in dissection buffer containing 40 μg/ml of Liberase ...
-
bioRxiv - Microbiology 2024Quote: ... then incubated twice with a final concentration of 50 μg/mL DNase I (Roche, Basel, Switzerland) and RNase A (Roche ...
-
bioRxiv - Cell Biology 2020Quote: ... 1% Triton X-100, 150mM NaCl, 50mM Tris pH 7.5, 10mM MgCl2, 5mM NaF, 1mM sodium orthovanadate, and Roche EDTA-free protease inhibitor cocktail). Unstimulated control cells were lysed in solution by adding an equal volume of 2x cold lysis buffer for a final volume of 500 μL ...
-
bioRxiv - Neuroscience 2020Quote: DNA from the patient and her parents was extracted from 100 μl of EDTA-anticoagulated whole blood using MagNA Pure (Roche Diagnostics, West Sussex, UK) and used for subsequent analyses.
-
bioRxiv - Molecular Biology 2021Quote: ... 150 mM NaCl, 1 mM EDTA, 1% v/v Triton X-100, 10% v/v glycerol, 1x cOmplete protease inhibitor cocktail [Roche], and 1x PhosSTOP [Roche]). Cells were scraped off ...
-
bioRxiv - Neuroscience 2020Quote: DNA from the patient and his parents was extracted from 100 μl of EDTA-anticoagulated whole blood using MagNA Pure (Roche Diagnostics, West Sussex, UK) and used for subsequent analyses.
-
bioRxiv - Neuroscience 2021Quote: ... whereas mouse primary cortical cultures and activated T lymphocytes were lysed in lysis buffer (50 mM Tris, 150 mM NaCl, 1% Triton X-100) containing protease and phosphatase inhibitors (Roche, New York, NY, USA). Total protein contents were quantified using the BCA assay (Pierce) ...
-
bioRxiv - Genomics 2022Quote: ... 0.1% Tween-20, 0,5% Triton X−100, 1 mM DTT, 1× protease inhibitors cocktail, complete EDTA-free, Roche and antifoam B, Sigma, 1:5000). The obtained extract was clarified by filtration through 1.6 μm GD/X Glass Microfiber syringe filters (25 mm ...
-
bioRxiv - Plant Biology 2019Quote: ... 300 mM sucrose, 1% Triton X-100) containing 1x protease inhibitor cocktail (Pierce, Rockford, IL) and a phosphatase inhibitor (PhosSTOP, Roche Applied Science, Penzberg, Germany). Protein extracts were centrifuged at 1,500 rpm for 5 min and the supernatant was re-centrifuged at 12,000 rpm for 10 min ...
-
bioRxiv - Cell Biology 2021Quote: Cells were lysed in Tris-Buffered Saline (TBS) + 1% Triton X-100 with EDTA and protease and phosphatase inhibitor cocktails (Roche Diagnostics, Florham Park, NJ). To remove insoluble materials ...
-
bioRxiv - Neuroscience 2024Quote: ... resuspended in 150 μL buffer C (50 mM Tris pH 8, 5 mM EDTA, 1% SDS, 100 mM NaCl, 1x Roche complete mini protease inhibitors) and incubated on ice for 10 min ...
-
bioRxiv - Biochemistry 2024Quote: ... 250 mg of powder was thawed at 4°C followed by resuspension in 1 mL of HIP buffer (40 mM HEPES-KOH pH 7.5, 110 mM KOAc, 2 mM MgCl2, 1% Triton X-100, 0.1% Tween, 1x protease inhibitor cocktail [Roche], 1% solution P, 1 mM DTT). The lysate was next passed through a Whatman 25 mm GD/X Disposable filter (Cat No ...
-
bioRxiv - Cell Biology 2024Quote: ... Nuclei were pelleted at 800g for 10 min at 4°C and washed twice in buffer 1 and once in freezing buffer (1M Tris pH 8, 100% glycerol, 100mM MgAc2, 0.5M EDTA, 1M DTT, 1x PTase inhibitor cocktail Roche, 20U/µl SUPERase In Ambion). Nuclei were resuspended in freezing buffer ...
-
bioRxiv - Plant Biology 2022Quote: ... 1/100 cDNA dilutions were analysed using a LightCycler®480 and LightCycler®480 SYBR Green I Master (Roche, Welwyn Garden City, UK) following manufacturer’s instructions and cycling conditions of pre-incubation 95°C for 5 min ...
-
bioRxiv - Physiology 2024Quote: Total protein was extracted from whole brain samples by homogenizing in 100 µL of 1X RIPA buffer containing protease inhibitors (cOmplete Mini Protease Inhibitor Cocktail, Hoffmann La Roche Limited, Mississauga, ON, Canada) and prepared for Western blotting exactly as previously described (Dindia et al. ...
-
bioRxiv - Cell Biology 2022Quote: ... and lysed in ice-cold lysis buffer (20 mM Tris-HCl, pH 7.4, 150 mM NaCl, 10% Glycerol, 1% Triton X-100, cOmpleteTM protease inhibitor cocktail tablet from Roche and a PhosSTOP tablet from Roche). Lysates containing 500-1000 μg protein (made up to 500 μl by lysis buffer ...
-
bioRxiv - Neuroscience 2021Quote: ... 300 mM NaCl, 30 mM imidazole, 1 M urea, 1% v/v Triton X-100, 5 mM beta-mercaptoethanol, with Roche EDTA-free cOmplete protease inhibitor) at room temperature ...
-
bioRxiv - Immunology 2021Quote: ... concentrations measured in 100 ul of basolateral medium incubated with the reaction mixture of a cytotoxicity detection kit (Sigma-Aldrich, Roche, Saint Louis, MO, USA) following the manufacturer’s instructions ...